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盖杨叶片高频植株再生体系建立

安徽农业科学 2016年第11期44卷 209-211页

作者：冯连荣辽宁省杨树研究所辽宁盖州115213

[目的]利用生物技术手段提高盖杨抗寒性并扩大其栽培区域。[方法]以盖杨叶片为外植体,研究不同激素组合对盖杨叶片分化及生根的影响,建立盖杨叶片高频植株再生体系。[结果]盖杨不定芽分化最适培养基配方为MS+6-BA 0.5 mg/L+NAA 0.20 mg... 详细信息

[目的]利用生物技术手段提高盖杨抗寒性并扩大其栽培区域。[方法]以盖杨叶片为外植体,研究不同激素组合对盖杨叶片分化及生根的影响,建立盖杨叶片高频植株再生体系。[结果]盖杨不定芽分化最适培养基配方为MS+6-BA 0.5 mg/L+NAA 0.20 mg/L+蔗糖25 g/L+琼脂6.0 g/L,p H 6.5,不定芽再生频率为96.67%,平均分化芽数20.3个;最适生根培养基配方为1/2MS+IBA0.3 mg/L+蔗糖20 g/L+琼脂6.0 g/L,p H 6.0,生根率可达100%,平均生根数15.8条。[结论]该研究建立了盖杨高效组织培养再生系统,为该杨树品种的快速无性繁殖和基因的遗传转化提供了依据。

关键词：盖杨叶片再生体系

Complete response of gallbladder cancer treated with gemcitabine and cisplatin chemotherapy combined with durvalumab:A case report and review of literature

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 226-232页

作者： Kai Chen Xu feng Yuan Shi Xin-Lin Li Zheng-rong Shi Xiang Lan Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Gallbladder cancer(GBC)is the most common and aggressive subtype of biliary tract cancer(BTC)and has a poor prognosis.A newly developed regimen of gemcitabine,cisplatin,and durvalumab shows promise for the treatment of advanced ***,the efficacy of this treatment for GBC remains *** SUMMARY In this report,we present a case in which the triple-drug regimen exhibited marked effectiveness in treating locally advanced GBC,thus leading to a long-term survival benefit.A 68-year-old man was diagnosed with locally advanced GBC,which rendered him ineligible for curative *** three cycles of therapy,a partial response was *** one year of combined therapy,a clinical complete response was successfully *** maintenance therapy with durvalumab monotherapy resulted in a disease-free survival of 9 months for the *** patient experienced tolerable toxicities of reversible grade 2 nausea and *** adverse events were observed in the patient throughout the entirety of the *** The combination of gemcitabine and cisplatin chemotherapy with durvalumab was proven to be an effective treatment approach for advanced GBC,with manageable adverse *** research is warranted to substantiate the effectiveness of the combined regimen in the context of GBC.

关键词： Gallbladder cancer Chemotherapy Immunotherapy Durvalumab Complete response Case report

2种启动子驱动下的VaCBF3基因植物表达载体构建

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沈阳农业大学学报 2011年第5期42卷 559-564页

作者：冯连荣王占斌宋立志辽宁省杨树研究所辽宁盖州115200 东北林业大学林学院哈尔滨150040

为了探索山葡萄CBF3基因(VaCBF3)对杨树的遗传转化的影响,及组成型启动子和诱导型启动子对转基因植株生长的影响,分别构建由2种类型启动子调控的植物表达载体。根据GenBank上公布的rd29A基因序列设计特异性引物,利用PCR方法从拟南芥基因... 详细信息

为了探索山葡萄CBF3基因(VaCBF3)对杨树的遗传转化的影响,及组成型启动子和诱导型启动子对转基因植株生长的影响,分别构建由2种类型启动子调控的植物表达载体。根据GenBank上公布的rd29A基因序列设计特异性引物,利用PCR方法从拟南芥基因组DNA中克隆rd29A基因启动子片段,利用限制性内切酶通过酶切、连接构建由组成型启动子35S和诱导型启动子rd29A基因启动子驱动的VaCBF3基因植物表达载体,利用冻融法完成重组质粒对根癌农杆菌LBA4404的转化。试验结果表明,所克隆的rd29A基因启动子片段序列分析表明该启动子片段长度为945bp,与GenBank中基因序列同源性达99%,具有DRE、ABRE、TATA box和CAAT box4个完整的顺式作用元件,具有启动子功能。经过酶切、连接成功地将目的基因与载体质粒连接,并将重组质粒导入至根癌农杆菌LBA4404中,为下一步利用农杆菌介导法对杨树的遗传转化及研究2种启动子的启动效率奠定了一定的基础。

关键词：启动子 rd29A 35S VaCBF3基因植物表达载体

人工神经网络阻抑动力学光度法同时测定对苯二胺和间苯二胺

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分析试验室 2010年第4期29卷 45-48页

作者：傅应强田丽陈宁生冯连荣安徽工程科技学院生物化学工程系芜湖241000 安徽工程科技学院电气工程系芜湖241000

在硫酸性介质中,Fe(Ⅲ)能够催化H2O2氧化甲基红褪色反应,对苯二胺和间苯二胺都能阻抑该催化氧化褪色反应的速度,两者对Fe(Ⅲ)催化H2O2氧化甲基红褪色反应阻抑作用不具有加和性,根据这一现象,用人工神经网络处理非线性体系的优势进行数... 详细信息

在硫酸性介质中,Fe(Ⅲ)能够催化H2O2氧化甲基红褪色反应,对苯二胺和间苯二胺都能阻抑该催化氧化褪色反应的速度,两者对Fe(Ⅲ)催化H2O2氧化甲基红褪色反应阻抑作用不具有加和性,根据这一现象,用人工神经网络处理非线性体系的优势进行数据处理,从而建立了一种新的测定对苯二胺和间苯二胺混合物的人工神经网络阻抑动力学光度法。对6组混合样品进行测定,回收率均在95%～105%之间。该方法运用于实验室水样的分析。

关键词：人工神经网络对苯二胺间苯二胺阻抑动力学光度法

Correlations of the expression of Cx43,SCFFBXW7,p-cyclin E1(Ser73),p-cyclin E1(Thr77)and p-cyclin E1(Thr395)in colon cancer tissues

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 207-213页

作者： rong-Gang Luan Ming-Da Liu Zi-feng Deng Cong-Lan Lu Mei-Ling Yu Ming-Yu Zhang rong Liu Ran An You-liang Yao Dong-Bei Guo Yong-Xing Zhang Lei Zhao Department of Gastrointestinal Surgery Air Force Hospital of Eastern TheaterAnhui Medical UniversityNanjing 230032Jiangsu ProvinceChina State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthXiamen UniversityXiamen 361102Fujian ProvinceChina Trauma Surgery Air Force Hospital of Eastern TheaterAnhui Medical UniversityNanjing 230032Jiangsu ProvinceChina Department of Orthopaedics Air Force Hospital of Eastern TheaterAnhui Medical UniversityNanjing 230032Jiangsu ProvinceChina

BACKGROUND Previous cellular studies have demonstrated that elevated expression of Cx43 promotes the degradation of cyclin E1 and inhibits cell proliferation through ***,reduced expression results in a loss of this capacity to facilitate cyclin E *** ubiquitination and degradation of cyclin E1 may be associated with phosphorylation at specific sites on the protein,with Cx43 potentially enhancing this process by facilitating the phosphorylation of these critical *** To investigate the correlation between expression of Cx43,SKP1/Cullin1/F-box(SCF)FBXW7,p-cyclin E1(ser73,thr77,thr395)and clinicopathological indexes in colon *** Expression levels of Cx43,SCFFBXW7,p-cyclin E1(ser73,thr77,thr395)in 38 clinical colon cancer samples were detected by immunohistochemistry and were analyzed by statistical methods to discuss their *** Positive rate of Cx43,SCFFBXW7,p-cyclin E1(Ser73),p-cyclin E1(Thr77)and p-cyclin E1(Thr395)in detected samples were 76.32%,76.32%,65.79%,5.26%and 55.26%*** expressions of these proteins were not related to the tissue type,degree of tissue differentiation or lymph node ***43 and SCFFBXW7(r=0.749),p-cyclin E1(Ser73)(r=0.667)and p-cyclin E1(Thr395)(r=0.457),SCFFBXW7 and p-cyclin E1(Ser73)(r=0.703)and p-cyclin E1(Thr395)(0.415)were correlated in colon cancer(P<0.05),and expressions of the above proteins were positively correlated in colon *** Cx43 may facilitate the phosphorylation of cyclin E1 at the Ser73 and Thr195 sites through its interaction with SCFFBXW7,thereby influencing the ubiquitination and degradation of cyclin E1.

关键词： Colon cancer Cx43 SCFFBXW7 Phosphorylation of cyclin E1 Sites of cyclin E1 Correlation analysis

Kangfuxin solution alleviates esophageal stenosis after endoscopic submucosal dissection:A natural ingredient strategy

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World Journal of Gastroenterology 2025年第1期31卷 110-125页

作者： Xin Zhou Dan Ma Yi-Xiang He Jing Jin Hong-lian Wang Yun-feng Wang Fan Yang Jian-Qin Liu Jie Chen Zhi Li Department of Spleen and Stomach Diseases the Affiliated Traditional Chinese Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina The Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Digestive System Diseases of Luzhou City The Affiliated Traditional Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Gastroenterology Changhai HospitalNaval Medical UniversityShanghai 20082China Research Center of Integrated Chinese and Western Medicine The Affiliated Traditional Chinese Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina School of Integrated Traditional Chinese and Western Clinical Medicine North Sichuan Medical CollegeNanchong637100Sichuan ProvinceChina

BACKGROUND Esophageal stricture ranks among the most significant complications following endoscopic submucosal dissection(ESD).Excessive fibrotic repair is a typical pathological feature leading to stenosis after *** To examine the effectiveness and underlying mechanism of Kangfuxin solution(KFX)in mitigating excessive fibrotic repair of the esophagus *** Pigs received KFX at 0.74 mL/kg/d for 21 days after esophageal full circumferential *** examinations occurred on days 7 and 21 *** vitro,recombinant transforming growth factor(TGF)-β1(5 ng/mL)induced a fibrotic microenvironment in primary esophageal fibroblasts(pEsF).After 24 hours of KFX treatment(at 1.5%,1%,and 0.5%),expression ofα-smooth muscle actin-2(ACTA2),fibronectin(FN),and type collagen I was *** signaling was analyzed,including TGF-β1,Smad2/3,and phosphor-smad2/3(p-Smad2/3).RESULTS Compared to the Control group,the groups treated with KFX and prednisolone exhibited reduced esophageal stenosis,lower weight loss rates,and improved food tolerance 21 d after *** treatment,Masson staining revealed thinner and less dense collagen fibers in the submucosal ***,the expression of fibrotic effector molecules was notably ***,KFX downregulated the transduction levels of fibrotic functional molecules such as TGF-β1,Smad2/3,and p-Smad2/*** vitro,pEsF exposed to TGF-β1-induced fibrotic microenvironment displayed increased fibrotic activity,which was reversed by KFX treatment,leading to reduced activation of ACTA2,FN,and collagen *** 1.5%KFX treatment group showed decreased expression of p-Smad 2/3 in TGF-β1-activated *** KFX showed promise as a therapeutic option for post-full circumferential esophageal ESD strictures,potentially by suppressing fibroblast fibrotic activity through modulation of the TGF-β1/Smads signaling pathway.

关键词： Kangfuxin solution Natural component Endoscopic submucosal dissection Esophagus stricture Fibrosis

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根癌农杆菌介导杨树遗传转化的影响因素

西北林学院学报 2015年第3期30卷 120-126页

作者：冯连荣宋立志张妍彭儒胜赵大根辽宁省杨树研究所辽宁盖州115200

针对根癌农杆菌介导杨树遗传转化过程中杨树受体系统的建立、质粒转化载体的构建、目的基因转化和转化体选择4部分影响因子进行综述。影响因子包括杨树基因型、外植体类型等内部因素,培养基、培养条件、vir基因诱导物、抗生素等外部因... 详细信息

针对根癌农杆菌介导杨树遗传转化过程中杨树受体系统的建立、质粒转化载体的构建、目的基因转化和转化体选择4部分影响因子进行综述。影响因子包括杨树基因型、外植体类型等内部因素,培养基、培养条件、vir基因诱导物、抗生素等外部因素。总结了在实际操作中应该注意的问题,为更好地利用根癌农杆菌介导法开展杨树遗传转化,提高转化效率提供借鉴和参考。

关键词：根癌农杆菌杨树遗传转化影响因素

微血管阻塞形态与急性心肌梗死临床预后相关性

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临床军医杂志 2022年第5期50卷 463-469页

作者：高光仁冯连荣李毅张军天津医科大学沧州市中心医院心内科河北沧州061000 天津医科大学沧州市中心医院神经内科河北沧州061000 北部战区总医院心血管内科辽宁沈阳110016

目的探讨微血管阻塞(MVO)形态与急性心肌梗死(AMI)患者临床预后的相关性。方法选取自2018年10月至2020年12月于天津医科大学沧州市中心医院首次诊断为AMI且发病14 d内行心脏磁共振检查提示有MVO的92例患者为研究对象。收集患者的年龄、... 详细信息

目的探讨微血管阻塞(MVO)形态与急性心肌梗死(AMI)患者临床预后的相关性。方法选取自2018年10月至2020年12月于天津医科大学沧州市中心医院首次诊断为AMI且发病14 d内行心脏磁共振检查提示有MVO的92例患者为研究对象。收集患者的年龄、性别、实验室检查指标、介入指标等临床资料。以GE后处理工作站测量,获得最大MVO角度(maxMVOA)、最大厚度(maxMVOT)、距心外膜(或室间隔右室侧)最小深度(minMVOD)及心肌厚度(TM),计算MVO厚度指数(MTI)。研究终点为发生主要不良心脑血管事件(MACCE),包括全因死亡、再发心肌梗死、再次冠状动脉血运重建、因心力衰竭再入院及卒中的复合终点。结果随访时间534(262,775)d。随访期间,58例患者未发生MACCE事件(无MACCE组);34例(37.0%)患者发生MACCE事件(MACCE组),其中,2例(2.2%)发生全因死亡,2例(2.2%)发生再发心肌梗死,3例(3.3%)发生再次血运重建,2例(2.2%)发生卒中,30例(32.6%)因心力衰竭再入院;无失访。MACCE组梗死面积[IS,以%左室容积(%LV)表示]、maxMVOA及MVO(%LV)高于无MACCE组,差异均有统计学意义(P0.05)。多因素Cox回归分析结果显示,maxMVOA是发生MACCE(风险比=1.010,95%可信区间1.001~1.019,P=0.026)的独立预测因子。受试者工作特征曲线下面积(AUC)显示,maxMVOA、MVO(%LV)及MVO(%IS)对MACCE的预测价值,AUC分别为0.77、0.63和0.61,maxMVOA优于其余两个指标。结论maxMVOA对合并MVO的急性心肌梗死患者发生MACCE有良好的预测价值,可以作为常规测量MVO的指标。

关键词：心肌梗死微循环阻塞形态预后

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H型高血压对脑白质疏松症严重程度的影响

中国实验诊断学 2020年第9期24卷 1435-1438页

作者：冯连荣高光仁王磊董爱勤沧州市中心医院神经内科河北沧州061000 沧州市中心医院心内科河北沧州061000

目的评价H型高血压对脑白质疏松症(LA)严重程度的影响。方法前瞻性顺序入选2016年3月至2017年3月沧州市中心医院神经内科脑白质疏松症患者,按照脑白质疏松程度Wahlund分级将患者分为轻中度脑白质疏松组和重度脑白质疏松组,比较两组患者... 详细信息

目的评价H型高血压对脑白质疏松症(LA)严重程度的影响。方法前瞻性顺序入选2016年3月至2017年3月沧州市中心医院神经内科脑白质疏松症患者,按照脑白质疏松程度Wahlund分级将患者分为轻中度脑白质疏松组和重度脑白质疏松组,比较两组患者一般临床资料,以二元Logistic回归模型分析影响脑白质疏松严重程度的危险因素。随访1年,以全因死亡及新发脑血管病为主要研究终点。结果共计入选患者864例,其中男性535人(62%),平均年龄(68.3±3.2)岁,失访12人,共计852(98.6%)人完成最终随访。重度脑白质疏松组的年龄,同型半胱氨酸,痴呆史,高血压史,H型高血压史,吸烟史显著高于轻中度脑白质疏松组(P<0.05)。年龄、痴呆史及H型高血压是脑白质疏松的独立危险因素。随访1年发现重度脑白质疏松组全因死亡率显著高于轻中度脑白质疏松组,而两者新发缺血性脑血管病方面无显著差异。结论H型高血压是脑白质疏松症的独立危险因素,与脑白质疏松症严重程度相关。

关键词： H型高血压脑白质疏松症高同型半胱氨酸血症