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Identification and Function of MicroRNAs Encoded by Herpesviruses

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Virologica Sinica 2008年第6期23卷 459-472页

作者： Zhi-qiang Bai Xiu-fen lei Lin-ding Wang Shou-jiang Gao Tumor Virology Group State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Tumor Virology Program Greehey Children＇s Cancer Research Institute and Department of Pediatrics The University of Texas Health Science Center at San Antonio San Antonio Texas USA

MicroRNAs (miRNAs) play important roles in eukaryotes,plants and some viruses. It is increasingly clear that miRNAs-encoded by viruses can affect the viral life cycle and host physiology. Viral miRNAs could repress the innate and adaptive host immunity,modulate cellular signaling pathways,and regulate the expression of cellular and viral genes. These functions facilitate viral acute and persistent infections,and have profound effects on the host cell survival and disease progression. Here,we discuss the miRNAs encoded by herpesviruses,and their regulatory roles involved in virus-host interactions.

关键词： MicroRNA (miRNA) Herpesviruses Latency and replication

The Status and Associated Factors of Successful Aging among Older Adults Residing in Longevity Areas in China

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Biomedical and Environmental Sciences 2016年第5期29卷 347-355页

作者： SHI Wen Hui ZHANG Hong Yan ZHANG Juan LYU Yue Bin Melanie Sereny Brasher YIN Zhao Xue LUO Jie Si HU Dong Sheng fen lei SHI Xiao Ming Division of NCD Control and Community Health Chinese Center for Disease Control and Prevention Beijing 102206 China Department of Epidemiology and Health Statistics College of Public Health Zhengzhou University Zhengzhou 450001 Henan China Department of Sociology and Anthropology Department of Human Development and Family Studies University of Rhode Island Kingston RI02881 USA Department of Preventive Medicine Shenzhen University School of Medicine Shenzhen Guangdong 518060 Guangzhou China Department of Psychological Medicine Yong Loo Lin School of Medicine National University of Singapore 119077 Singapore Institute of Environment Health and Related Product Safety Chinese Center for Disease Control and Prevention Beijing 100020 China

Objective This study aims to assess the status of successful aging （SA） in longevity areas in China and explore multiple factors associated with SA among the young-old and oldest-old. Methods A total of 2296 elderly people aged 65 and older were interviewed in the longevity areas sub-sample of the Chinese Longitudinal Healthy Longevity Survey （CLHLS） in 2012. Baseline assessments included a researcher-administered questionnaire, physical examination, and laboratory testing. A logistic regression model was used to identify factors associated with SA. Results The prevalence of SA was 38.81% in the CLHLS in 2012. There were significant differences between ages groups, with SA compromising 56.85% among _〉65 years group and 20.31% among 〉100 years group （X2trend=126.73, P〈O.01）. The prevalence of SA among females was 33.59%, which was significantly lower than that among males （45.58%）（X2gender=33.65, P〈0.05）. In the regression analysis, having anemia ~OR=0.744, 95% CI： 0.609-0.910）, poor lifestyle （OR=0.697, 95% CI： 0.568-0.854）, poor sleep quality （OR=0.558, 95% CI： 0.456-0.682）, and central obesity （OR=0.684, 95% CI： 0.556-0.841） were the main factors associated with SA. The promoting SA rate decreased as age increased, and the group of 65-79 years had higher odds than the other age group. Conclusion Preventing central obesity, improving sleep quality and promoting healthy lifestyle may contribute to achieve SA among the elderly.

关键词： Successful aging Older adults Evaluation Associated factors

Effect and safety of sorafenib in patients with intermediate hepatocellular carcinoma who received transarterial chemoembolization: A retrospective comparative study

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World Journal of Clinical Cases 2018年第5期6卷 74-83页

作者： Xue-fen lei Yang Ke Tian-Hao Bao Hao-Ran Tang Xue-Song Wu Zhi-Tian Shi Jie Lin Zhi-Xian Zhang Hou Gu Lin Wang Department of Medical Oncology The Second Affiliated Hospital of Kunming Medical University Department of Hepatobiliary Surgery The Second Affiliated Hospital of Kunming Medical University The Mental Health Center of Kunming Medical University Department of Hepatobiliary Surgery The Second Affiliated Hospital of Kunming Medical University Department of Gastro- enterological Surgery The Second Affiliated Hospital of Kunming Medical University

AIM To evaluate the safety and efficacy of sorafenib plus transarterial chemoembolization(TACE) treatment for intermediate hepatocellular carcinoma(HCC).METHODS Sixty-seven patients with intermediate-stage [Barcelona Clinic liver cancer stage B(BCLC-B)] HCC who were treated with sorafenib plus TACE or TACE alone between 2009 and 2011 were included in the study. Follow-up was until 2014 or patient death. Two groups were defined in the experiment: The experimental group, treated with sorafenib plus TACE, and the control group, treated with standard TACE *** The Kaplan-Meier survival analysis showed that the median overall survival(m OS) of the experimental group was 35.2 mo, while that of the control group was 22.0 mo(P fenib plus TACE showed higher incidence rates of rash, hand-foot syndrome(HFS), and hypertension(P fenib plus TACE treatment for BCLC-B HCC significantly prolonged the m OS of patients compared to TACE treatment alone. The most common toxicities with sorafenib were rash(31.6%), HFS(39.5%) and hypertension(31.6%), but there were no intolerable adverse events. The Cox multivariate analysis showed that the survival of patients with BCLC-B HCC depended on the Child-Pugh classification, tumor diameter, and treatment with sorafenib plus TACE compared to TACE alone.

关键词： Sorafenib Hepatocellular carcinoma Transarterial chemoembolization Overall survival Adverse reaction

Preparation,Characterization of Hydrophobic Drug in Combine Loaded Chitosan/Cyclodextrin/Trisodium Citrate Nanoparticles and in vitro Release Study

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Chemical Research in Chinese Universities 2012年第1期28卷 166-170页

作者： JI Jin-gou, ZHANG Jing-fen, HAO Shi-lei, WU Dan-jun, LIU Li and XU Yi College of Chemistry and Chemical Engineering, University of Chongqing, Chongqing 400030, P. R. China College of Chemistry and Chemical Engineering University of Chongqing Chongqing 400030

Chitosan/cyclodextrin/trisodium citrate（CS/CD/TSC） nanoparticles with ibuprofen（IBU） loaded were prepared via the ionic cross-linking method, with trisodium citrate selected as the cross-linking agent. The drug-loading capacity, particle size, zeta potential and surface morphology of the obtained nanoparticles were inves tigated. The results show a good drug-loading capacity. The prepared nanoparticles were spherical in shape with an average size of 293.7 nm and a zeta potential of ＋30.72 mV. The in vitro release studies show that the controlled re lease of IBU from the nanoparticles was followed. The drug release from CS/β-CD/TSC nanoparticles followed non-Fickian or anomalous diffusion.

关键词： Chitosan Cyclodextrin Nanoparticle Ibuprofen(IBU)

一株深海来源苏云金芽孢杆菌SWJS07所产蛋白酶的分离纯化及性质研究

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现代食品科技 2015年第8期31卷 165-170页

作者：赵谋明郇惠杰雷芬芬崔春华南理工大学轻工与食品学院广东广州510640

利用超滤、硫酸铵盐析、DEAE Sepharose Fast Flow阴离子交换柱层析、Sephadex G-75分子筛柱层析对一株南海深海来源菌苏云金芽孢杆菌(***)SWJS07所产蛋白酶进行分离纯化,纯化后经SDS-PAGE鉴定达到电泳纯,相对分子质量为37.0 k Da,酶的... 详细信息

利用超滤、硫酸铵盐析、DEAE Sepharose Fast Flow阴离子交换柱层析、Sephadex G-75分子筛柱层析对一株南海深海来源菌苏云金芽孢杆菌(***)SWJS07所产蛋白酶进行分离纯化,纯化后经SDS-PAGE鉴定达到电泳纯,相对分子质量为37.0 k Da,酶的比活力提高了6.39倍,回收率为37.14%。研究其酶学性质表明,该蛋白酶最适催化温度为55℃,在30℃~45℃下稳定性较高,保温300 min残留酶活在80%以上;最适p H 6.5,在p H 6.0~9.0蛋白酶稳定,4℃放置24 h残留酶活在80%以上;2 m M Ca2+、Mn2+对蛋白酶有不同程度的激活作用,而Hg2+、Cd2+、Al3+则强烈地抑制蛋白酶活;当在蛋白酶中添加2 m M Ca2+、Mn2+时,其最适催化温度分别为60℃和55℃,蛋白酶活分别提高了32.86%和28.35%,60℃保温30 min相对酶活基本保持不变,与纯酶(相对酶活残留21.02%)相比蛋白酶的热稳定性显著提高;EDTA-Na2可强烈抑制蛋白酶活,推测该蛋白酶属于金属蛋白酶。

关键词：苏云金芽孢杆菌SWJS07 蛋白酶分离纯化酶学性质

A Comparison of the Cultural Connotations between Chinese and English Animal Idioms and Translation

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芜湖职业技术学院学报 2010年第2期12卷 56-60页

作者：何雷玢北京化工大学北京100029

自人类文明诞生以来,与人类的物质生活息息相关的动物不但进入了人类的语言世界,并且逐步形成了与实践经验相关的文化含义。在不同的文化背景中,同样的动物却具有不同的文化含义。只有不断加深异质文化的相互理解,这种文化含义的差异才... 详细信息

自人类文明诞生以来,与人类的物质生活息息相关的动物不但进入了人类的语言世界,并且逐步形成了与实践经验相关的文化含义。在不同的文化背景中,同样的动物却具有不同的文化含义。只有不断加深异质文化的相互理解,这种文化含义的差异才能得到认识。

关键词：文化背景文化含义差异语言

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产耐盐蛋白酶深海细菌的分离鉴定

现代食品科技 2015年第3期31卷 50-54页

作者：赵谋明舒会崔春雷芬芬唐雪鹭华南理工大学轻工与食品学院广东广州510640

本文采用高盐富集、复筛发酵及耐盐稳定性实验的方式,从深海海泥中筛选产耐盐蛋白酶的深海海洋细菌。从深海海洋微生物中共筛选出25株耐盐菌,在这25株菌株中有14株在酪蛋白平板上能产生较大的水解圈;通过发酵复筛及耐盐稳定性实验,筛选... 详细信息

本文采用高盐富集、复筛发酵及耐盐稳定性实验的方式,从深海海泥中筛选产耐盐蛋白酶的深海海洋细菌。从深海海洋微生物中共筛选出25株耐盐菌,在这25株菌株中有14株在酪蛋白平板上能产生较大的水解圈;通过发酵复筛及耐盐稳定性实验,筛选得到一株酶活性能高且耐盐稳定性好的菌株,将其编号为SWJSS3;对菌株SWJSS3进行16SrDNA的鉴定并初步研究了盐浓度对产酶情况和酶稳定性的影响。菌株SWJSS3在0-10%（m/V）的盐浓度条件下均能生长,在0-10%的盐浓度下,菌液OD600的吸光值范围为0.08-1.98;通过发酵复筛其所产生的蛋白酶酶活在盐浓度为1%时达最高,为233.56±2.16U/mL;所产蛋白酶在终浓度为15%（m/V）的NaCl溶液下,混匀4℃保存1h,残余酶活为初始酶活的40.70±2.06%,继续存放一段时间到第9h,酶活基本保持不变;通过16SrDNA鉴定其为铜绿假单胞菌,与PseudomonasaeruginosaRP2816SrDNA的相似性达到99%以上。

关键词：深海海洋细菌蛋白酶耐盐稳定性分离鉴定

15000Nm钻机试验台测试系统设计与应用

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煤田地质与勘探 2008年第4期36卷 74-77页

作者：代粉蕾国家安全生产西安勘探设备检测检验中心

由于煤矿坑道钻机钻进能力的提高,原有的钻机试验台已不能满足测试转矩超过6 000 Nm钻机的需要,为此,新建了煤矿坑道钻机试验台。新系统包括硬件和软件2部分,其最大测试转矩达到15 000 Nm,最大给进力/起拔力达到300 kN;采用智能化仪器,... 详细信息

由于煤矿坑道钻机钻进能力的提高,原有的钻机试验台已不能满足测试转矩超过6 000 Nm钻机的需要,为此,新建了煤矿坑道钻机试验台。新系统包括硬件和软件2部分,其最大测试转矩达到15 000 Nm,最大给进力/起拔力达到300 kN;采用智能化仪器,减少了计算量,提高了工作效率;软件系统具有扩展性强、复用性高、可进行灵活控制的特点;系统具有断电保护功能。新测试台与原试验台的比对试验表明,2个试验台得出的结果一致。

关键词：钻机试验台测试系统设计

从“健康体检”到“健身行动”——学校层面运用事实与数据提升管理品质的实践探索

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江苏教育研究（实践）（B版） 2017年第2期 23-28页

作者：张蕾芬南京外国语学校仙林分校

"3-6年级学生学习状况的调查"是一种大规模的行动研究,也是管理工作的新探索。调查聚焦学生需求,以学生为中心,让学习更加贴近学生需要。调查数据及分析诊断让老师关注和反思自身日常教育,改进教育行为;让学校的教育管理基于数据与事实... 详细信息

"3-6年级学生学习状况的调查"是一种大规模的行动研究,也是管理工作的新探索。调查聚焦学生需求,以学生为中心,让学习更加贴近学生需要。调查数据及分析诊断让老师关注和反思自身日常教育,改进教育行为;让学校的教育管理基于数据与事实,提高了管理效益,提升了学校教育质量。

关键词：学习状况调查数据分析教育管理