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Differentiation of human bone marrow precursor cells into neuronal-like cells after transplantation into canine spinal cord organotypic slice cultures

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Chinese Medical Journal 2012年第22期125卷 4049-4054页

作者： fei zhi-qiang, xiong jian-yi chen lei shen hui-yong ngo stephanie wang jeffrey wang da-ping shenzhen Second Peoples Hosp Dept Orthopaed Shenzhen Key Lab Tissue Engn Res Ctr Shenzhen 518035 Guangdong Peoples R China Sun Yat Sen Univ Mem Hosp Dept Orthopaed Guangzhou 510120 Guangdong Peoples R China Univ Calif Los Angeles UCLA Spine Ctr Santa Monica CA 90404 USA

Background Treatments to regenerate different tissue involving the transplantation of bone marrow derived mesenchymal precursor cells are anticipated. Using an alternative methods, in vitro organotypic slice culture method, would be useful to transplant cells and assessing the effects. This study was to determine the possibility of differentiating human bone marrow precursor cells into cells of the neuronal lineage by transplanting into canine spinal cord organotypic slice cultures. Methods Bone marrow aspirates were obtained from posterior superior iliac spine （PSIS） of patients that had undergone spinal fusion due to a degenerative spinal disorder. For cell imaging, mesenchymal precursor cells （MPCs） were pre-stained with PKH-26 just before transplantation to canine spinal cord slices. Canine spinal cord tissues were obtained from three adult beagle dogs. Spinal cords were cut into transverse slices of 1 mm using tissue chopper. Two slices were transferred into 6-well plate containing 3 ml DMEM with antibiotics. Prepared MPCs （lx104） were transplanted into spinal cord slices. On days 0, 3, 7, 14, MPCs were observed for morphological changes and expression of neuronal markers through immunofluorescence and reverse transcription-polymerase chain reaction （RT-PCR）. Results The morphological study showed： spherical cells in the control and experiment groups on day 0; and on day 3, cells in the control group had one or two thick, short processes and ones in the experiment group had three or four thin, long processes. On day 7, these variously-sized processes contacted each other in the experiment group, but showed typical spindle-shaped cells in the control group. Immunofluorescence showed that PKH-26（＋） MPCs stained positive for NeuN （＋） and GFAP（＋） in experimental group only. Also RT-PCR showed weak expression of β-tubulin III and GFAP. Conclusions Human bone marrow mesenchymal precursor cells （hMPCs） have the potential to differentiate into the neuronal like cells i

关键词： differentiation mesenchymal precursor cell organotypic slice culture spinal cord

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The first data release(DR1) of the LAMOST regular survey

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Research in Astronomy and Astrophysics 2015年第8期15卷 1095-1124页

作者： A-Li Luo yong-Heng Zhao Gang Zhao Li-Cai Deng Xiao-Wei Liu yi-Peng Jing Gang wang Hao-Tong Zhang jian-Rong Shi Xiang-Qun Cui Yao-Quan Chu Guo-ping Li Zhong-Rui Bai Yue Wu Yan Cai Shu-Yun Cao Zi-Huang Cao jeffrey L.Carlin Hai-Yuan chen jian-Jun chen Kun-Xin chen Li chen Xue-lei chen Xiao-Yan chen ying chen Norbert Christlieb Jia-Ru Chu chen-Zhou Cui yi-Qiao Dong Bing Du Dong-Wei Fan lei Feng jian-Ning Fu Peng Gao Xue-fei Gong Bo-Zhong Gu Yan-Xin Guo Zhan-Wen Han Bo-Liang He Jin-Liang Hou yong-hui Hou Wen Hou Hong-Zhuan Hu Ning-sheng Hu Zhong-Wen Hu zhi-ying Huo lei Jia Fang-Hua jiang Xiang jiang zhi-Bo jiang Ge Jin Xiao Kong Xu Kong Ya-Juan lei Ai-Hua Li Chang-Hua Li Guang-Wei Li Hai-Ning Li jian Li Qi Li Shuang Li Sha-Sha Li Xin-Nan Li Yan Li yin-Bi Li Ye-ping Li Yuan Liang Chien-cheng Lin Chao Liu Gen-Rong Liu Guan-Qun Liu zhi-Gang Liu Wen-zhi Lu Yu Luo yin-Dun Mao Heidi Newberg Ji-Jun Ni Zhao-Xiang Qi yong-Jun Qi Shi-yin shen Huo-Ming Shi Jing Song yi-Han Song Ding-qiang Su Hong-Jun Su Zheng-Hong Tang Qing-sheng Tao Yuan Tian dan wang da-Qi wang Feng-fei wang Guo-Min wang Hai wang Hong-Chi wang jian wang Jia-Ning wang jian-Ling wang jian-ping wang Jun-Xian wang lei wang Meng-Xin wang Shou-Guan wang Shu-Qing wang Xia wang Ya-Nan wang You wang Yue-fei wang You-Fen wang Peng Wei Ming-zhi Wei Hong Wu Ke-fei Wu Xue-Bing Wu Yu-Zhong Wu Xiao-Zheng Xing Ling-Zhe Xu Xin-Qi Xu Yan Xu Tai-sheng Yan De-Hua Yang Hai-Feng Yang hui-Qin Yang Ming Yang Zheng-Qiu Yao yong Yu hui Yuan Hai-Bo Yuan Hai-Long Yuan Wei-Min Yuan Chao Zhai En-Peng Zhang Hua-Wei Zhang jian-Nan Zhang Li-Pin Zhang Wei Zhang yong Zhang Yan-Xia Zhang Zheng-Chao Zhang Ming Zhao Fang Zhou Xu Zhou Jie Zhu yong-Tian Zhu Si-cheng Zou Fang Zuo National Astronomical Observatories Chinese Academy of Sciences Department of Astronomy Peking University Shanghai Astronomical Observatory Chinese Academy of Sciences Nanjing Institute of Astronomical Optics & Technology National Astronomical ObservatoriesChinese Academy of Sciences University of Science and Technology of China Department of Physics Applied Physics and AstronomyRensselaer Polytechnic InstituteTroyNY 12180USA Department of Physics and Astronomy Earlham CollegeRichmondIN 47374USA Universitt Heidelberg Zentrum für AstronomieLandessternwarteKnigstuhl 1269117 HeidelbergGermany Department of Astronomy Beijing Normal University Yunnan Observatories Chinese Academy of Sciences Purple Mountain Observatory Chinese Academy of Sciences

The Large sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST） general survey is a spectroscopic survey that will eventually cover approximately half of the celestial sphere and collect 10 million spectra of stars, galaxies and QSOs. Objects in both the pilot survey and the first year regular survey are included in the LAMOST DR1. The pilot survey started in October 2011 and ended in June 2012, and the data have been released to the public as the LAMOST Pilot data Release in August 2012. The regular survey started in September 2012, and completed its first year of operation in June 2013. The LAMOST DR1 includes a total of 1202 plates containing 2 955 336 spectra, of which 1 790 879 spectra have observed signalto-noise ratio（SNR） ≥ 10. All data with SNR ≥ 2 are formally released as LAMOST DR1 under the LAMOST data policy. This data release contains a total of 2 204 696 spectra, of which 1 944 329 are stellar spectra, 12 082 are galaxy spectra and 5017 are quasars. The DR1 not only includes spectra, but also three stellar catalogs with measured parameters： late A,FGK-type stars with high quality spectra（1 061 918 entries）, A-type stars（100 073 entries）, and M-type stars（121 522 entries）. This paper introduces the survey design, the observational and instrumental limitations, data reduction and analysis, and some caveats. A description of the FITS structure of spectral files and parameter catalogs is also provided.

关键词： techniques: spectroscopic survey—data release—catalog

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