检索结果-南通市图书馆

Bone marrow mesenchymal stem cell-induced autophagy ameliorates TNBS-induced experimental colitis by downregulating the NLRP3 inflammasome

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

BIOCELL 2023年第12期47卷 2627-2639页

作者： JINJIN FU XIAOYUE FENG JUAN WEI XIANG GENG YU GONG FENGDONG LI SHAOHUA ZHUANG JIN HUANG fangyu wang Department of Gastroenterology The Affiliated Changzhou No.2 People’s HospitalNanjing Medical UniversityChangzhouChina Department of Gastroenterology Jinling HospitalNanjing Medical UniversityNanjingChina

This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells(BM-MSCs)may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid(TNBS),specifically through autophagy ***:BM-MSCs were collected from BALB/c mice for subsequent *** study employed cell counting kits(CCK-8)to investigate the impact of the MSC-conditioned medium(M medium)on the proliferation of RAW264.7 *** GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic *** gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction(qRT-PCR).Western blot analysis was employed to determine the presence of a binding interaction between NOD-like receptor protein 3(NLRP3)and ***,a colitis mouse model was established by TNBS *** disease activity score was assessed regularly,and histological and morphometric analyses were performed on colonic *** serum cytokines were identified using an enzyme-linked immunosorbent ***:BM-MSCs significantly promoted the proliferation of *** vitro lipopolysaccharide(LPS)-stimulated RAW264.7 cells,treated with BM-MSCs,triggered autophagy and inhibited cytokine mRNA ***,in LPS-induced RAW264.7,BM-MSCs enhanced the Beclin1 protein expression and the microtubule-associated protein 1 light chain 3(LC3)-II to LC3-I ratio while suppressing the protein levels of NLRP3 and apoptosis-associated speck-like protein(ASC).Nevertheless,3-methyladenine(3-MA),an inhibitor of autophagy,prevented the impact of BM-MSCs by reducing the levels of NLRP3 and ASC proteins,suggesting that autophagy triggered the inhibition of the NLRP3 *** comparison to the mice in the TNBS group,the mice in the TNBS+MSC group displayed a more acute form of colitis,and the IL1βand IL18 cytokines in their serum were

关键词： RAW264.7 3-Methyladenine(3-MA) IL1β IL18

Protective and Adverse Roles of DDX3X in Different Cell Types in Nonalcoholic Steatohepatitis Progression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research 2024年第3期6卷 561-574页

作者： Suzhen Yang Lin Zhou Tianming Zhao Hanlong Zhu Tingting Luo Kang Jiang Xiaoxiao Shi Chunyan Chen Han Zhang Si Zhao Xiaoping Zou Yuzheng Zhuge fangyu wang Lei wang Mingzuo Jiang Bing Xu Department of Gastroenterology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolJiangsuNanjing210002China. Department of Gastroenterology and Hepatology the Affiliated Jinling Hospital of Nanjing University Medical SchoolJiangsuNanjing210002China School of Medicine Northwest UniversityShaanxiXi’an710069China. Department of Gastroenterology Nanjing Drum Tower HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeJiangsuNanjing210008China.

Persistent hepatic cellular metabolic stress and liver inflammatory stimuli are key signatures of nonalcoholic steatohepatitis(NASH).DDX3X is a vital molecule involved in cell fate decisions in both pro-survival stress granule(SG)and pro-death NOD-like receptor family pyrin domain containing 3(NLRP3)inflammasome assembly in response to stress ***,the role of DDX3X in NASH remains *** characterized the cell type-specific roles of DDX3X in *** liver tissues from NASH patients and normal control subjects were collected to assess DDX3X expression and *** steatohepatitis models were constructed by feeding macrophage-specific DDX3X knockout(DDX3^(XΔMφ)),hepatocyte-specific DDX3X knockout(DDX3X^(Δhep)),and wild-type control(DDX3X^(fl/fl))mice a high-fat and high-cholesterol(HFHC)diet,a methionine-and choline-deficient(MCD)diet,and a high-fat/high-iron/high-fructose/high-cholesterol,low-methionine,and choline-deficient(HFHIHFHC-MCD)*** study demonstrated that DDX3X was predominantly expressed in macrophages and hepatocytes in control liver tissues,and its expression was down-regulated in patients or mice with *** to DDX3X^(fl/fl) littermates,DDX3^(XΔMφ)mice showed improved liver histology in nutritional steatohepatitis *** of macrophage DDX3X inhibited NLRP3 inflammasome-mediated pyroptosis,causing anti-inflammatory M2 polarization and alleviating hepatocyte steatohepatitic ***3X^(Δhep) mice developed marked steatohepatitis in multiple nutritional steatohepatitis models compared to DDX3X^(fl/fl) ***3X-deleted hepatocytes showed impaired SG assembly,leading to increased sensitivity and intolerance to metabolic stimulation and resultant *** conclusion,DDX3X plays opposite roles in different cell types during the progression of NASH.A better understanding of the cell-specific differences in the crosstalk between SG formation and NLRP3 activation is crucial for developing pr

关键词： NLRP3 impaired inhibited

Resolving the lineage relationship between malignant cells and vascular cells in glioblastomas

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2023年第2期14卷 105-122页

作者： fangyu wang Xuan Liu Shaowen Li Chen Zhao Yumei Sun Kuan Tian Junbao wang Wei Li Lichao Xu Jing Jing Juan wang Sylvia MEvans Zhiqiang Li Ying Liu Yan Zhou Department of Neurosurgery Zhongnan hospital of Wuhan UniversityFrontier Science Center for Immunology and MetabolismMedical Research InstituteThe RNA InstituteCollege of Life SciencesWuhan UniversityWuhan 430071China Department of Neurology The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430014China Skaggs School of Pharmacy Department of MedicineDepartment of PharmacologyUniversity of California at San DiegoLa JollaCA 92093USA

Glioblastoma multiforme(GBM),a highly malignant and heterogeneous brain tumor,contains various types of tumor and non-tumor *** GBM cells can trans-differentiate into non-neural cell types,including mural cells or endothelial cells(ECs),to support tumor growth and invasion remains *** we generated two genetic GBM models de novo in immunocompetent mouse brains,mimicking essential pathological and molecular features of human ***-tracing and transplantation studies demonstrated that,although blood vessels in GBM brains underwent drastic remodeling,evidence of trans-differentiation of GBM cells into vascular cells was barely ***,GBM cells could promiscuously express markers for mural cells during ***,single-cell RNA sequencing showed that patterns of copy number variations(CNVs)of mural cells and ECs were distinct from those of GBM cells,indicating discrete origins of GBM cells and vascular ***,single-cell CNV analysis of human GBM specimens also suggested that GBM cells and vascular cells are likely separate *** than expansion owing to trans-differentiation,vascular cell expanded by proliferation during ***,cross-lineage trans-differentiation of GBM cells is very unlikely to occur during *** findings advance understanding of cell lineage dynamics during gliomagenesis,and have implications for targeted treatment of GBMs.

关键词： glioblastoma mural cells endothelial cells trans-differentiation lineage tracing single-cell sequencing copy number variation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

糖尿病脑病小鼠中胆汁酸代谢及信号通路研究

糖尿病脑病小鼠中胆汁酸代谢及信号通路研究

作者： fangyu wang 天津大学

学位级别：硕士

目的随着人们对代谢性疾病的广泛关注,已有诸多研究表明在一些代谢性疾病中,胆汁酸以及胆汁酸信号通路已经成为潜在的治疗靶点,例如:糖尿病、肥胖以及胆结石病。糖尿病脑病是糖尿病的一种并发症,是一种复杂的代谢性疾病,伴随着中枢神经... 详细信息

目的随着人们对代谢性疾病的广泛关注,已有诸多研究表明在一些代谢性疾病中,胆汁酸以及胆汁酸信号通路已经成为潜在的治疗靶点,例如:糖尿病、肥胖以及胆结石病。糖尿病脑病是糖尿病的一种并发症,是一种复杂的代谢性疾病,伴随着中枢神经和脑功能损伤,表现为认知功能障碍,使得患老年痴呆的风险增加。对糖尿病脑病的发病机制以及临床上的治疗方法已有很多报道,主要包括对脑组织中的各种神经细胞及神经递质的研究,以及临床上采用的中枢神经刺激的治疗方法。但是,至今仍没有胆汁酸代谢在糖尿病脑病的发生和发展过程中的作用的相关报道。所以,本课题的研究目的是在糖尿病脑病这种复杂的疾病模型中探索胆汁酸代谢及其信号通路。有文献表明,糖尿病的发生会导致迷走神经的病变,而迷走神经对于胆汁酸的代谢和信号通路的影响尚没有系统的研究。作为寻找糖尿病脑病的潜在预防治疗靶点的第一步,我们试着探寻迷走神经在胆汁酸体内平衡中的调节作用。方法为了定量检测生物组织和体液中的胆汁酸浓度,进而对生物体内的胆汁酸代谢进行分析,首先建立了简单有效的液相色谱-质谱联用(LC-MS)方法。该方法经过了线性、稳定性、准确度、精密度、检测线(LOD)、定量限(LOQ)、回收率、基质效应等验证。生物体内组织和体液中胆汁酸的提取采用简便的四步提取法:1.乙腈沉淀蛋白;2.震荡提取;3.离心后上清干燥;4.50%甲醇-水(双蒸水)复溶,样品前处理完成后等待进样检测。糖尿病脑病动物模型的建立采用C57BL/6小鼠,实验组小鼠长期喂养高脂饮食,糖尿病组和糖尿病脑病组小鼠经单次注射链脲霉素(STZ)诱导。模型构建后期,用水迷宫试验(Morris water maze test)判断小鼠疾病阶段,将小鼠分成四组,分别为正常组(NC)、高脂饮食组(HFD)、糖尿病组(unDCD)及糖尿病脑病组(DCD)。模型建立成功后,将小鼠麻醉并收集小鼠肝脏、肠道及结肠。用液相色谱-质谱联用方法(LC-MS)定量测定肝脏和肠道中胆汁酸的浓度;并采用逆转录聚合酶链式反应实时荧光定量技术(RT-PCR)和蛋白免疫印迹技术(Western Blot)检测肝脏和肠道中与胆汁酸相关的基因表达情况;采用定量PCR技术检测分析结肠内容物中细菌总量的变化和各类细菌百分比含量的变化。为了探究迷走神经对胆汁酸代谢以及信号通路的调控作用,本研究继而建立了大鼠(Wistar)膈下迷走神经切断模型,模型构建成功后,对大鼠进行麻醉,收集胆汁、血液、肝脏、肠道以及结肠。采用液相色谱-质谱联用技术定量测定胆汁、肝脏以及血清中胆汁酸浓度。并采用逆转录聚合酶链式反应实时荧光定量技术和蛋白免疫印迹技术检测肝脏和肠道中胆汁酸相关的基因表达情况。结果糖尿病脑病小鼠模型建立过程中,观察到高脂饲料喂养持续五周后,四组小鼠中的体重差异显现,高脂饮食组(HFD)小鼠体重相比于正常饮食组(NC)小鼠体重显著增加了19%,然而糖尿病(unDCD)和糖尿病脑病(DCD)组小鼠体重却有所减轻,尤其糖尿病脑病组小鼠体重比正常组小鼠显著下降了12%。饲养第六周时,三组实验组小鼠的空腹血糖水平相比正常对照组升高,糖尿病组和糖尿病脑病组小鼠血糖相比于高脂喂养组小鼠血糖均显著升高了约1.3倍。血脂检测中,实验组小鼠的总胆固醇含量相比正常组显著升高;甘油三酯的含量在糖尿病脑病小鼠中显著升高,在糖尿病小鼠中升高但无统计学意义;相比正常组,低密度脂蛋白含量在糖尿病和糖尿病脑病小鼠中均显著性升高,高密度脂蛋白在糖尿病小鼠中显著升高,在脑病小鼠中增加不明显(约44%)。在用于观察小鼠学习及记忆能力的水迷宫实验中,得到如下结果:在为期五天的观察中,从第三天开始小鼠的记忆能力差异显现,糖尿病脑病组小鼠逃避潜伏时间显著长于其它三组小鼠;首次到达平台的时间显著高于正常组,相比糖尿病组小鼠慢了约3s;穿越平台的次数相比高脂饮食组和糖尿病组减少一次,相比正常组小鼠减少四次。糖尿病脑病模型研究中胆汁酸浓度定量检测的结果显示,同正常组(NC)以及高脂饮食组(HFD)相比,在糖尿病(unDCD)和糖尿病脑病(DCD)小鼠的肝脏和肠道中总胆汁酸的含量均明显升高,尤其在糖尿病脑病小鼠中总胆汁酸的升高存在统计学差异。糖尿病小鼠肝脏中总胆汁酸的升高主要是由于如下几类胆汁酸含量的增加引起的,TMCA增加了259%,TMDCA增加了153%,TCDCA增加了342%,然而几种非结合型胆汁酸显著降低,CA降低60%,βMCA降低38%,DCA降低80%,导致肝脏中非结合型胆汁酸总量降低了55%。糖尿病脑病小鼠肝脏中不仅总胆汁酸含量升高,初级胆汁酸和结合型胆汁酸的总量也分别升高,有几种胆汁酸浓度变化明显,TMCA增加了212%,TCA增加了102%,TCDCA增加了279%,CA增加了39%,CDCA增加了46%,isoCDCA增加了403

关键词：胆汁酸肠道菌糖尿病脑病迷走神经

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2017年第4期9卷 302-314页

作者： Yamu Li Wen wang fangyu wang Qiushuang Wu Wei Li Xiaoling Zhong Kuan Tian Tao Zeng Liang Gao Ying Liu Shu Li Xiaobing Jiang Guangwei Du Yan Zhou Hubei Key Laboratory of Cell Homeostasis College of Life Sciences at Wuhan University Wuhan 430072 China Department of Neurosurgery The Tenth Affiliated Hospital Tongji University Shanghai 200072 China Department of Neurosurgery Fujian Medical University Union Hospital Fuzhou 350001 China Medical Research Institute Wuhan University Wuhan 430072 China Department of Neurosurgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Integrative Biology and Pharmacology University of Texas Health Science Center at Houston Houston TX 77225 USA

GUoblastoma multiforme （GBM） is a highly invasive brain tumor with limited therapeutic means and poor prognosis. Recent stud- ies indicate that glioma-initiating ceUs/gUoma stem ceils （GICs/GSCs） may be responsible for tumor initiation, infiltration, and recurrence. GICs could aberrantly employ molecular machinery balancing self-renewal and differentiation of embryonic neural precursors. Here, we find that paired related homeobox 1 （PRRX1）, a homeodomain transcription factor that was previously reported to control skeletal development, is expressed in cortical neural progenitors and is required for their self-renewal and proper differentiation. Further, PRRX1 is overrepresented in gUoma samples and labels GlCs. Gtioma celts and GlCs depleted with PRRX1 could not propagate in vitro or form tumors in the xenograft mouse model. The GIC self-renewal function regulated by PRRX1 is mediated by dopamine D2 receptor （DRD2）. PRRX1 directly binds to the DRD2 promoter and transactivates its expression in GICs. Blockage of the DRD2 signaling hampers GIC self-renewal, whereas its overexpression restores the propagating and tumorigenic potential of PRRXl-depleted GlCs. Finally, PRRX1 potentiates GICs via DRD2-mediated extracetlutar signal-related kinase （ERK） and AKT activation. Thus, our study suggests that therapeutic targeting the PRRX1-DRD2-ERK/AKT axis in GICs is a promising strategy for treating GBMs.

关键词： paired related homeobox 1 dopamine D2 receptor glioma-initiating cells glioblastoma

Bimetallic Pd-Ni core-shell nanoparticles catalysts for the Suzuki reaction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2014年第9期7卷 1337-1343页

作者： Ji Xiang Peng Li Hanbao Chong Li Feng fangyu Fu Zhuang wang Shilin Zhang Manzhou Zhu School of Chemistry and Chemical Engineering Anhui University Hefei 230601 China

A simple and efficient solution-based method for the synthesis of Pd-Ni bimetallic nanoparticles （NPs） has been developed. A series of Pd-Ni bimetallic NPs were readily achieved by reduction of PdC12 and Ni（acac）2 （acac = acetyl- acetonate） in the presence of oleylamine （OAm）, oleic acid （OA） and benzyl alcohol. Furthermore, by using high-resolution transmission electron microscopy （HRTEM）, energy-dispersive spectrometry （EDS） mapping and X-ray diffraction （XRD）, we demonstrate that the as-prepared Pd-Ni bimetallic NPs have core-shell structures with a Pd-rich core and a Ni-rich shell. In addition, the as-obtained Pd-Ni bimetallic NPs with varying compositions show excellent catalytic activities in the Miyaura-Suzuki reaction. When the nickel molar percentage was 0.23 to 0.65, the conversion with the as-obtained Pd-Ni bimetallic catalysts was above 90%. It is believed that this strategy can be employed to produce a variety of other well-defined core-shell type multimetallic nanostructures.

关键词： Pd Ni bimetallic NPs catalytic activities

A Study of Diagnosis METHOD for Turbocharger Abnormal Noise

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Study of Diagnosis METHOD for Turbocharger Abnormal Noise

The application value of functional magnetic resonance in the disease evaluation of patients with ulcerative colitis

2019中国汽车工程学会年会

作者： Qiu Yongjin wang fangyu Ren Dong Dongfeng Motor Corporation Technical Center Xiangyang Technician College

Basedon the problem of abnormal noise of a turbocharger model in acceleration condition,the study starts from the spectrum characteristics of the abnormal *** with subjective evaluation and analysis of transmission paths and noise and vibration frequency of various components of engine compartment,this paper elaborates on the excitation characteristics of the *** to the generation mechanism of abnormal noise,considering factors such as spatial layout,performance matching and cost constraints,a pneumatic buffer has been set reasonably to eliminate or reduce the influence of turbocharger abnormal noise on riding comfort during *** integrating principle,analysis and improvement,a set of diagnosis methods for abnormal noise of turbocharger is *** being improved and verified,the proposed methods have been applied.

关键词： turbocharger abnormal noise diagnosis method pneumatic buffer acceleration

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The application value of functional magnetic resonance in th...

Effect of Different Proportions of Leymus chinensis in Diet on Plasma Enzymes Activities of Wulong Geese

2019中国中西医结合学会消化内镜学专业委员会第一届第四次学术交流会

作者： Chang Liu Yifei Zhou fangyu wang Jinling Hosp Dept Gastroenterology Nanjing UnivSch Med

Background : We have discovered that patients who suffered from ulcerative colitis(UC) may also accompany with anxiety and depression, which affect the quality life. According to previous researches, anxiety and depression are usually along with brain functional changes. In order to observe the relationship between brain functional changes and anxiety, depression especially in UC patients, a functional magnetic resonance imaging(f-MRI) technology was applied to conduct the study. The purpose of this study was to investigate the spontaneous brain activities and influence factors in UC patients compared with healthy people. Methods: 27 patients diagnosed as UC were recruited from jinling hospital, 17 of them and 17 matched healthy controls with similar gander, age and years of education were underwent a functional magnetic resonance imaging(f-MRI) 3.0 T scan. Regional homogeneity(ReHo) was used to measure the synchronism of regional brain activities. T test, Chi-square test, pearson correlation were used to analyse the data. Results: On one hand, a significant decreased ReHo values in Parietalnf(except supramarginal gyrus and angular convolution) and Parietalup were found in UC patients compared with healthy controls. Moreover, there were significant negative correlations between ReHo values and SAS scores(Pearson correlation, p=0.019,0.05). On the other hand, there is statistical significance in SAS(42.72±12.543) and SDS(38.75(33.75-53.13)) scores between UC patients and healthy controls. Moreover, years of education revealed statistical significance in SAS scores(c2=9.047,p=0.009<0.05) between UC patients and healthy controls. No statistical significance was found in SDS scores between several factors in UC patients and healthy people. Conclusion: Abnormal brain functional changes exists in UC patients and they are more likely to be anxio

关键词： Brain-gut-microbiota axis Ulcerative colitis Functional magnetic resonance imaging ReHo Anxiety Depression

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Northeast Agricultural University(English Edition) 2008年第1期15卷 28-33页

作者： wang Baowei wang Yachao ZHANG Qian YANG Zhigang ZHANG Xuhui LONG fangyu wang Lei High Quality Waterfowl Research Institute Qingdao Agricultural University Qingdao 266109 Shandong China

Eight-month-old Wulong geese were fed with the diet with different proportions of Leymus chiesensis at the same energy and protein level. The activities of plasma Alkaline Phosphatase （AKP）, Acid Phosphatase （ACP）, Choline Esterace （CHE）, Lactic Dehydrogenase （LDH）, Glutamicoxalacetic Transaminase （GOT） and Glutamatepyruvate Transaminase （GPT） were determined and then their correlations with fiber digestibility and the apparent digestibility of Ca, R Met and Cys were analyzed. The results showed that the activity of AKP took on a significant downtrend as the increasing of Leymus chinensis proportion （P〈0.05）. There was a negative correlation between the AKP activity and the digestibility of crude fiber （CF）, neutral detergent fiber （NDF） and acid detergent fiber （ADF）（P〈0.05）. However, there was a significant positive correlation between the AKP activity and the apparent digestibility of Ca, Met and Cys （P〈0.05）. Moreover, the activities of the other five enzymes had no significant correlation with the digestibility of CF, NDF, ADF, and the apparent digestibility of Ca, Met and Cys （P〉0.05）.

关键词： Wulong geese Leymus chinensis activities of plasma enzymes digestibility correlation