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低密度脂蛋白促进腹膜间皮细胞转分化及细胞外基质蓄积

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中华肾脏病杂志 2013年第1期29卷 44-49页

作者：房艳辉蒋兰萍陈丽萌李雪梅李学旺中国医学科学院北京协和医院肾内科北京100730

目的探讨高糖作用下低密度脂蛋白（LDL）对人腹膜间皮细胞（HPMC）转分化及细胞外基质（ECM）蓄积的影响。方法（1）将HPMC随机分为对照组、LDL组（100mg／L）和LDL（100mg／L）＋LDL受体阻断剂乳铁蛋白（100mg／L）组，共培养24h。细... 详细信息

目的探讨高糖作用下低密度脂蛋白（LDL）对人腹膜间皮细胞（HPMC）转分化及细胞外基质（ECM）蓄积的影响。方法（1）将HPMC随机分为对照组、LDL组（100mg／L）和LDL（100mg／L）＋LDL受体阻断剂乳铁蛋白（100mg／L）组，共培养24h。细胞免疫荧光法观察HPMC细胞上LDL受体的表达；油红O染色法观察HPMC对LDL的摄取情况。（2）将HPMC分别加入不同浓度LDL（0、25、50、100mg／L）培养24h，倒置相差显微镜下观察细胞形态的改变，细胞免疫荧光法观察细胞内OL平滑肌肌动蛋白（α-SMA）表达。（3）将静止细胞分为对照组（5．6mmol／L葡萄糖）、甘露醇组（M，2．18％甘露醇）、低糖组（LG，30mmol／L葡萄糖）、高糖组（HG，120mmol／L葡萄糖）和高糖＋LDL组（HG＋LDL，120mmol／L葡萄糖＋100mg／LLDL），共培养48h。实时定量PCR法检测***、E，cadherin和1型纤溶酶原激活物抑制物（PA1-1）mRNA的表达；Western印迹法检测α-SMA蛋白表达；EliSA法检测细胞培养上清中I型胶原（Col I）和PA1-1蛋白含量。结果（1）免疫荧光观察到LDL可以刺激HPMC细胞表达LDL受体。油红O染色法观察到HPMC能摄取LDL进入细胞内，加入乳铁蛋白能抑制LDL进入细胞内。（2）倒置相差显微镜下观察到，随LDL浓度增高，细胞问连接逐渐趋于松散，细胞呈现明显梭形成纤维细胞样形态；胞质内α-SMA荧光强度逐渐增强。与对照组相比，HG＋LDL组α-SMAmRNA和蛋白表达显著上调（均P〈0．05），E-cadherinmRNA表达显著下调（P〈0．05）。上述指标在HG组与HG＋LDL组、HG组与对照组之间表达量差异无统计学意义。（3）EliSA法检测结果显示，与HG组和对照组相比，HG＋LDL组细胞上清中Col I蛋白含量[（19．27±0．17）μg/L比（14．09±0．30）μg／L、（14．81±0．91）μg／L，均P〈0．05]及PAI-1含量[（498．24±76．91）ng／L比（342．19±30．43）μg／L、（220．39＋33．82）ng/L，均P〈0．051均显著上升；同时PAI-1mRNA水平亦升高，与对照组差异有统计学意义（P=0．022）。结论HPMC通过LDL受体摄取LDL后，能在高糖作用下诱导HPMC发生转分化，通过上调PAI-1表达抑制ECM降解，导致ECM蓄积。

关键词：脂蛋白类 LDL 细胞转分化细胞外基质人腹膜间皮细胞

The Chinese Society of Clinical Oncology (CSCO): Clinical guidelines for the diagnosis and treatment of gastric cancer, 2023

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Cancer Communications 2024年第1期44卷 127-172页

作者： Feng-Hua Wang Xiao-Tian Zhang Lei Tang Qi Wu Mu-Yan Cai Yuan-fang li xiu-Juan Qu Hong Qiu Yu-Jing Zhang Jie-Er Ying Jun Zhang ling-Yu Sun Rong-Bo lin Chang Wang Hao liu Miao-Zhen Qiu Wen-Long Guan Sheng-Xiang Rao Jia-Fu Ji Yan Xin Wei-Qi Sheng hui-Mian Xu Zhi-Wei Zhou Ai-ping Zhou Jing Jin Xiang-lin Yuan Feng Bi Tian-Shu liu Han liang Yan-Qiao Zhang Guo-Xin li Jun liang Bao-Rui liu lin Shen Jin li Rui-Hua Xu Department of Medical Oncology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Gastrointestinal Oncology Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education)Peking University Cancer HospitalBeijingP.R.China Department of Radiology Peking University Cancer HospitalBeijingP.R.China Department of Endoscopy Center Peking University Cancer HospitalBeijingP.R.China Department of Pathology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center of Cancer MedicineGuangzhouGuangdongP.R.China Department of Gastric Surgery Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Medical Oncology The First Hospital of China Medical UniversityShenyangLiaoningP.R.China Department of Medical Oncology Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and TechnologyWuhanHubeiP.R.China Department of Radiotherapy Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Medical Oncology Zhejiang Cancer HospitalHangzhouZhejiangP.R.China Department of Medical Oncology Ruijin HospitalShanghai Jiaotong University School of MedicineShanghaiP.R.China Department of Surgical Oncology The Fourth Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiangP.R.China Department of Medical Oncology Fujian Cancer HospitalFuzhouFujianP.R.China Tumor Center The First Hospital of Jilin UniversityChangchunJilinP.R.China Department of General Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China Department of Radiology Zhongshan HospitalFudan UniversityShanghaiP.R.China Department of

The 2023 update of the Chinese Society of Clinical Oncology(CSCO)Clini-cal Guidelines for Gastric Cancer focuses on standardizing cancer diagnosis and treatment in China,reflecting the latest advancements in evidence-based medicine,healthcare resource availability,and precision *** updates address the differences in epidemiological characteristics,clinicopatho-logical features,tumor biology,treatment patterns,and drug selections between Eastern and Western gastric cancer *** revisions include a structured template for imaging diagnosis reports,updated standards for molecular marker testing in pathological diagnosis,and an elevated recommendation for neoadju-vant chemotherapy in stage III gastric *** advanced metastatic gastric cancer,the guidelines introduce new recommendations for immunotherapy,anti-angiogenic therapy and targeted drugs,along with updated management strategies for human epidermal growth factor receptor 2(HER2)-positive and deficient DNA mismatch repair(dMMR)/microsatellite instability-high(MSI-H)***,the guidelines offer detailed screening recommendations for hereditary gastric cancer and an appendix listing drug treatment regimens for various stages of gastric *** 2023 CSCO Clinical Guidelines for Gastric Cancer updates are based on both Chinese and international clinical research and expert consensus to enhance their applicability and relevance in clinical practice,particularly in the heterogeneous healthcare landscape of China,while maintaining a commitment to scientific rigor,impartiality,and timely revisions.

关键词： Chinese Society of Clinical Oncology(CSCO) gastric cancer diagnosis surgery neoadjuvant adjuvant radiotherapy chemotherapy targeted therapy immunotherapy

高糖诱导成肌细胞应激的低强度单色光调节的实验研究

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体育科学 2012年第8期32卷 40-48,54页

作者：李方晖刘承宜杨海平韦恩秀刘延莹华南师范大学体育科学学院激光运动医学实验室和民族体质与健康研究中心广东广州510006 肇庆学院体育与健康学院广东肇庆526061

背景和目的:低强度单色光(liL)疗法广泛应用于运动损伤防护。研究liL对高糖(HG)诱导的成肌细胞应激的调节作用及其机制。方法:C2C12成肌细胞在不同浓度的葡萄糖中培养,其中22.5和90.0mmol/L浓度分别作为正糖组(NG)和HG组。随后用强度为I... 详细信息

背景和目的:低强度单色光(liL)疗法广泛应用于运动损伤防护。研究liL对高糖(HG)诱导的成肌细胞应激的调节作用及其机制。方法:C2C12成肌细胞在不同浓度的葡萄糖中培养,其中22.5和90.0mmol/L浓度分别作为正糖组(NG)和HG组。随后用强度为I mW/cm2的发光二极管640±15nm红光(RLED)照射N天(I*N)(I=0.035,0.067;N=1,2,3)(每天15min)。细胞用浓度为1～100μmol/L的白藜芦醇(RES)在NG中预处理细胞M h(RES1-100/M)(M=6,12,24),随后更换为NG/HG继续培养72h。细胞的各个指标用常规方法检测。结果:1)NG组细胞的增殖速率最快。2)0.035*3不能调节NG组细胞增殖,但I*N(I=0.035,0.067;N=1,2,3)分别在第3和4天显著促进HG组细胞增殖。3)RES5-100/6、RES1-100/12和RES1-30/24显著促进HG组细胞增殖,且RES10/12显著促进NG组细胞增殖。4)0.035*3显著地增加了sirtuin 1(SIRT1)、锰超氧化物歧化酶(MnSOD)和肌源性胰岛素样生长因子(mIGF-1)mRNA转录,降低了叉头框蛋白3a(FOXO3a)、p27和细胞死亡介导因子(BIM)mRNA转录;RES10/12显著增加SIRT1和Mn-SOD mRNA转录,降低了p27mRNA转录,但对FOXO3a和BIM mRNA转录没有明显影响。5)HG在第4和72h分别增加和减少烟酰胺腺嘌呤二核苷酸辅酶与其还原形式比值(NAD+/NADH)及SIRT1蛋白表达,但分别被0.035*1和0.035*3所康复。结论:22.5mmol/L葡萄糖浓度组的C2C12成肌细胞处于增殖特异的内稳态,不能被红光调节,但可以被白藜芦醇促进。红光通过激活mIGF-1/SIRT1/FOXO3a信号通路促进90.0mmol/L浓度组C2C12成肌细胞增殖特异的内稳态建立。

关键词：弱光治疗成肌细胞细胞增殖白藜芦醇内稳态葡萄糖

Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients

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Chinese Medical Journal 2013年第12期126卷 2276-2280页

作者： ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing liN Hong-li liU Jian XIE Ru-juan WANG li-ning NI Zhao-hui liU Fu-you YIN Ai-ping XING Chang-ying WANG li SHI Wei liU Jian-she HE Ya-ni DING Guo-hua li Wen-ge WU Guang-li MIAO li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI yun-kai luO hui-min liN Shan CHEN meng-hua GONG li yang Yi-bin yang Xiao-ping li Ying WAN Jian-xin WANG Nian-song li Hai-ying XI Chun-sheng HAO li XU Yan fang Jing-ai liU Bi-cheng li Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng liU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG liang-bao Department of Nephrology State key Laboratory of Kidney Disease Chinese PLA General Hospital Beijing 100853 China Nephrology Institute of the Chinese People's liberation Army Chinese People's Liberation Army Key Laboratory of Nephrology Beijing 100853 China Department of Nephrology West China Hospital of Sichuan University Chengdu Sichuan 610041 China Department of Nephrology First Affiliated Hospital School of Medicine Zhejiang University Hangzhou Zhejiang 310003 China Department of Nephrology Zhongshan Hospital Fudan University Shanghai 200032 China Department of Nephrology First Affiiated Hospital of Sun Yat-sen University Guangzhou Guangdong 510080 China Department of Nephrology First Affiliated Hospital of Dalian Medical University Dalian Liaoning 116011 China Department of Nephrology First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang 830054 China Department of Nephrology First Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150001 China Department of Nephrology First Afftiated Hospital of China Medical University Shenyang Liaoning 110001 China Department of Nephrology Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200025 China Department of Nephrology Second Xiangya Hospital Central South University Changsha Hunan 410000 China Department of Nephrology First Affiliated Hospital of Medical College Xi'an Jiaotong University Xi'an Shaanxi 710061 China Department of Nephrology First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital) Nanjing Jiangsu 210029 China Department of Nephrology Sichuan Provincial People's Hospital Chengdu Sichuan 610072 China Department of Nephrology Guangdong Provincial People's Hospital Guangzhou Guangdong 510030 China Department of Nephrology Affliated Xiehe Hospital Tongji Medical College Huazhong Science and Technology University Wuhan Hubei 430022 China Department of Nephrology Daping Hospital Third Military Medi

Background Data on the epidemiology of hypertension in Chinese non-dialysis chronic kidney disease （CKD） patients are *** aim of the present study was to investigate the prevalence,awareness,treatment,and control of hypertension in the non-dialysis CKD patients through a nationwide,multicenter study in *** The survey was performed in 61 tertiary hospitals in 31 provinces,municipalities,and autonomous regions in China （except Hong Kong,Macao,and Taiwan）.Trained physicians collected demographic and clinical data and measured blood pressure （BP） using a standardized *** was defned as systolic BP ≥140 mmHg and/or diastolic BP ≥90 mmHg,and/or use of antihypertensive *** 〈140/90 mmHg and 〈130/80 mmHg were used as the 2 thresholds of hypertension *** multivariate logistic regression with adjustment for sex and age,we analyzed the association between CKD stages and uncontrolled hypertension in non-dialysis CKD *** The analysis included 8927 non-dialysis CKD *** prevalence,awareness,and treatment of hypertension in non-dialysis CKD patients were 67.3%,85.8%,and 81.0%,*** hypertensive CKD patients,33.1% and 14.1% had controlled BP to 〈140/90 mmHg and 〈130/80 mmHg,*** successive CKD stages,the prevalence of hypertension in non-dialysis CKD patients increased,but the control of hypertension decreased （P〈0.001）.When the threshold of BP 〈130/80 mmHg was considered,the risk of uncontrolled hypertension in CKD 2,3a,3b,4,and 5 stages increased 1.3,1.4,1.4,2.5,and 4.0 times compared with CKD 1 stage,respectively （P〈0.05）.Using the threshold of 〈140/90 mmHg,the risk of uncontrolled hypertension increased in advanced stages （P〈0.05）.Conclusions The prevalence of hypertension Chinese non-dialysis CKD patients was high,and the hypertension control was *** successive CKD stages,the risk of uncontrolled hypertension increased.

关键词： non-dialysis chronic kidney disease chronic kidney disease stages hypertension epidemiology

miR-342-5p Regulates Neural Stem Cell Proliferation and Differentiation Downstream to Notch Signaling in Mice

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miR-342-5p Regulates Neural Stem Cell Proliferation and Diff...

内蒙古自治区某三级医院住院患者常见医院感染的直接经济损失

中国神经科学学会第十二届全国学术会议

作者： fang Gao Yu-Fei Zhang Zheng-ping Zhang luo-An Fu xiu-li Cao Yi-Zhe Zhang Chen-Jun Guo Xian-Chun Yan Qin-Chuan yang Yi-yang Hu Xiang-hui Zhao Ya-Zhou Wang Sheng-Xi Wu Gong Ju Min-Hua Zheng Hua Han Institute of Neurosciences Department of Neurobiology Collaborative Innovation Center for Brain Science School of Basic Medicine Department of Medical Genetics and Developmental Biology Fourth Military Medical University Department of Spinal Surgery Honghui Hospital Xi’an Jiaotong University College of Medicine

Notch signaling is critically involved in neural development, but the downstream effectors remain incompletely understood. In this study, we cultured neurospheres from Nestin-Cre-mediated conditional Rbp-j knockout（Rbp-j c KO） and control embryos and compared their miRNA expression profiles using microarray. Among differentially expressed miRNAs, mi R-342-5 p showed upregulated expression as Notch signaling was genetically or pharmaceutically interrupted. Consistently, the promoter of the mi R-342-5 p host gene, the Ena-vasodilator stimulated phosphoproteinlike（Evl）, was negatively regulated by Notch signaling, probably through HES5. Transfection of mi R-342-5 p promoted the differentiation of neural stem cells（NSCs） into intermediate neural progenitors（INPs） in vitro and reduced the stemness of NSCs in vivo. Furthermore, mi R-342-5 p inhibited the differentiation of neural stem/intermediate progenitor cells into astrocytes, likely mediated by targeting GfaP directly. Our results indicated that mi R-342-5 p could function as a downstream effector of Notch signaling to regulate the differentiation of NSCs into INPs and astrocytes commitment.

关键词： Neural Stem Cell Neural progenitor Cell miR-342-5p downstreams

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中国感染控制杂志 2019年第12期18卷 1099-1105页

作者：刘卫平邢慧敏郭天慧许彬彬赵宇平杨永芳任伟杨丽芳杨慧君王萌内蒙古自治区人民医院医院感染控制科

目的对住院患者常见医院感染造成的直接经济损失进行研究,为增强医务人员医院感染防控意识,提升医院感染防控能力提供参考依据。方法回顾性调查2017年1月1日-12月31日内蒙古自治区某三级医院发生呼吸道医院感染、血流医院感染、消化道... 详细信息

目的对住院患者常见医院感染造成的直接经济损失进行研究,为增强医务人员医院感染防控意识,提升医院感染防控能力提供参考依据。方法回顾性调查2017年1月1日-12月31日内蒙古自治区某三级医院发生呼吸道医院感染、血流医院感染、消化道医院感染、手术部位感染、泌尿道医院感染的住院患者,按照配对标准1:1配对病例组(医院感染)和对照组(非医院感染),采用配对资料Wilcoxon检验分析医院感染造成的直接经济损失。结果最终匹配成功262对,每例患者由医院感染所造成的直接经济损失为31300元,其中重症监护病房(ICU)医院感染直接经济损失最大(100857元)。两组患者住院总费用、住院日数比较,差异均有统计学意义(均P<0.05);两组患者床位费、护理费、西药费、手术费、治疗费、化验费、检查费比较,差异均有统计学意义(均P<0.05),各项费用中以西药费最高(14378元)。两组患者不同医院感染部位造成的平均住院总费用和住院日数比较,差异均有统计学意义(均P<0.05)。不同医院感染部位的平均住院总费用由高至低依次为手术部位(47469元)、血液(42498元)、泌尿道(34598元)、消化道(33296元)、呼吸道(31058元)。病例组患者延长平均住院日数7 d,两组患者平均住院日数比较,差异有统计学意义(P<0.05)。结论住院患者发生医院感染后可导致医疗费用增加,平均住院日延长,给患者带来了较大损失。

关键词：医院感染直接经济损失病例对照研究住院日数

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利用呼吸舱测定肉鸡氨气排放的研究

畜牧兽医学报 2014年第2期45卷 249-254页

作者：张晓迪卢庆萍张宏福孟丽辉唐湘方中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室北京100193

本试验旨在利用呼吸舱测定白羽肉鸡氨气排放率(ER),并研究其排放趋势。选取1日龄、健康的爱拔益加肉鸡300只随机分配在3个呼吸舱中(公母各半),采用网上平养一次性清粪的饲养方式饲喂至42日龄。试验期间实时监测通风量和进气口、排气口... 详细信息

本试验旨在利用呼吸舱测定白羽肉鸡氨气排放率(ER),并研究其排放趋势。选取1日龄、健康的爱拔益加肉鸡300只随机分配在3个呼吸舱中(公母各半),采用网上平养一次性清粪的饲养方式饲喂至42日龄。试验期间实时监测通风量和进气口、排气口处的氨气浓度,计算氨气排放量。试验结果为:白羽肉鸡生长42d内平均每只鸡的氨气排放量为(2 777.78±55.58)mg,平均氨气排放率为(66.14±1.32)(mg·只-1)·d-1,上市体重为(2 530.95±57.74)g。肉鸡氨气排放在1~18d维持平稳,之后随日龄增加而增大。

关键词：肉鸡氨气排放

含贝达喹啉方案治疗44例痰菌阳性耐多药/广泛耐药肺结核24周疗效分析

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中国防痨杂志 2021年第11期43卷 1139-1145页

作者：裴异高静韬黄云辉何芳封文军杨晓云胡羽萌雷丽萍施利南华大学附属长沙中心医院肺六科 410007 首都医科大学附属北京胸科医院/北京市结核病胸部肿瘤研究所临床中心

目的评估含贝达喹啉(bedaquiline,Bdq)方案治疗菌阳耐多药肺结核(MDR-PTB)/准广泛耐药肺结核(pre-XDR-PTB)/广泛耐药肺结核(XDR-PTB)的有效性和安全性,为中国耐药肺结核患者使用Bdq提供临床数据支持。方法纳入2018年3月至2020年3月就诊... 详细信息

目的评估含贝达喹啉(bedaquiline,Bdq)方案治疗菌阳耐多药肺结核(MDR-PTB)/准广泛耐药肺结核(pre-XDR-PTB)/广泛耐药肺结核(XDR-PTB)的有效性和安全性,为中国耐药肺结核患者使用Bdq提供临床数据支持。方法纳入2018年3月至2020年3月就诊于南华大学附属长沙中心医院痰分枝杆菌培养阳性的MDR-PTB、pre-XDR-PTB和XDR-PTB患者44例,其中男性31例,女性13例,中位年龄38.2(26,49)岁,25例并发空洞。给予含贝达喹啉方案治疗,分析24周痰分枝杆菌培养阴转率和肺部空洞闭合率评估方案的有效性,分析不良事件(adverse event,AE)发生情况,尤其是QT间期延长的不良事件评估方案的安全性。结果 44例患者中,38例痰菌阴转成功,2例痰菌阴转失败,2例死亡,1例退组,1例失访,良好转归占比86.4%(38/44)。完成24周治疗患者第2、4、8、12、24周痰分枝杆菌培养阴转率分别为45.5%(20/44)、72.5%(29/40)、95.0%(38/40)、100.0%(40/40)和95.0%(38/40),痰菌阴转的中位时间为22 (18,59)d。并发空洞的25例患者中,23例完成24周治疗,12周和24周末空洞闭合率分别为39.1%(9/23)和82.6%(19/23)。29例患者(65.9%,29/44)累积报告了81次AE,72.8%(59/81)为1级或2级,与Bdq相关的AE主要为QT间期延长(18例,40.9%)。结论 MDR-PTB、pre-XDR-PTB和XDR-PTB患者使用含Bdq的治疗方案24周痰菌阴转率和空洞闭合率较高,安全性及耐受性良好。

关键词：结核肺泛耐药结核病结核抗多种药物性贝达喹啉临床方案

骨复合材料结合生物膜即刻修复兔下颌骨缺损(英文)

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中国组织工程研究与临床康复 2009年第21期13卷 4101-4104页

作者：方利华平金良孟祥勇温辉民湖州市中心医院口腔科浙江省湖州市313000 湖州市中心医院病理科浙江省湖州市313000 湖州师范学院医学院口腔系浙江省湖州市313000

背景:近年来虽然有用不同方法处理过的异体骨、异种骨及各种组织工程材料骨问世,但临床上对颌骨缺损后骨量不足的治疗仍然没有较理想的解决方法。目的:通过动物实验观察较大下颌骨缺损及伴牙脱位的下颌骨缺损后骨复合材料结合生物膜即... 详细信息

背景:近年来虽然有用不同方法处理过的异体骨、异种骨及各种组织工程材料骨问世,但临床上对颌骨缺损后骨量不足的治疗仍然没有较理想的解决方法。目的:通过动物实验观察较大下颌骨缺损及伴牙脱位的下颌骨缺损后骨复合材料结合生物膜即刻修复的效果。设计、时间及地点:对比观察动物实验,于2006-03/07在浙江大学动物实验室完成。材料:Bio-oss骨代材料与自体骨骨粉混合的比例为1∶1,重组人骨形成蛋白2冻干粉溶于自体新鲜血液的比例为0.25mg∶1mL。将骨粉混合物用含人骨形成蛋白的血液湿润,以容易粘到药匙上方便塑形为度。方法:取10只新西兰大白兔,在双侧下颌骨体部下缘各造成15mm×6mm×5mm连续性骨缺损,随机选取一侧植入骨复合材料加Bio-gide膜,该侧作为骨复合材料加Bio-gide膜组。另一侧缺损直接拉拢缝合作为空白对照组。其余30只新西兰大白兔为骨复合材料加Bio-gide膜及再植牙组,在一侧下颌骨下缘上方造成15mm×6mm×8mm的骨缺损伴牙脱位,植入骨复合材料加Bio-gide膜,并将脱位牙齿再植于原处。主要观察指标:大体观察植入材料部位和材料结合情况及有无成骨、再植牙松动等。X射线摄片和组织学观察骨缺损处新骨形成情况。结果:术后12周空白对照组下颌骨缺损处形成一较原截骨范围略小的骨缺损,骨复合材料加Bio-gide膜组下颌骨缺损区基本由新生骨组成,X射线观察骨缺损区密度接近正常骨组织,组织学观察骨植入物基本形成板状骨,骨复合材料加Bio-gide膜及再植牙组动物自体再植牙有17只无明显松动,X射线观察有13只根尖无透射区,组织学观察有13只出现替代性吸收。结论:该种骨复合材料结合生物膜即刻修复较大下颌骨缺损效果良好,在其上自体牙再植近期效果尚可。

关键词：下颌骨缺损骨复合材料 Bio-gide生物膜自体牙再植