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Insect Science 2017年第2期24卷 222-234页

作者： zhi-Wei Kang fang-Hua Liu Hong-gang Tian Meng Zhang Shan-Shan guo Tong-Xian Liu State Key Laboratory of Crop Stress Biology for the Arid Areas and Key Laboratory of Northwest Loess Plateau Crop Pest Managementof Ministry of Agriculture and Northwest A&F University Yangling Shaanxi China

The green peach aphid, Myzus persicae Sulzer （Hemiptera, Aphididae）, is an important cosmopolitan pest. Real time qRT-PCR has been used for target gene expression analysis on M. persicae. Using real time qRT-PCR, the expression levels are normalized on the basis of the reliable reference genes. However, to date, the stability of available reference genes has been insufficient. In this study, we evaluated nine candidate reference genes from M. persicae under diverse experimental conditions. The tested candidate genes were comprehensively ranked based on five alternative methods （RefFinder, geNorm, Normfinder, BestKeeper and the comparative ACt method）. 18s, Actin and ribosomal protein L27 （L27） were recommended as the most stable reference genes for M. persicae, whereas ribosomal protein L27 （L27） was found to be the least stable reference genes for abiotic studies （photoperiod, temperature and insecticide susceptibility）. Our finding not only sheds light on establishing an accurate and reliable normalization of real time qRT-PCR data in M. persicae but also lays a solid foundation for further studies of M. persicae involving RNA interference and functional gene research.

关键词： Myzus persicae real-time qRT-PCR reference gene stability treatment

TEM Study on the Formation Process of TiO_2 Nanotubes

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Chinese Chemical Letters 2003年第4期14卷 419-422页

作者： jing Wei ZHANG Xin Yong guo Zhen Sheng JIN Shun Li ZHANG jing fang ZHOU zhi Jun ZHANG Lab of Special Functional Materials Henan UniversityKaifeng 475001

The process, that the polycrystalline TiO2 powders were converted into TiO2 nanotubes, was observed with transmission electron microscope. The results obtained indicated that in concentrated NaOH aqueous solution, an... 详细信息

关键词： TiO2 nanotubes anisotropic swelling TEM.

Atmospheric Corrosion of Q235 Carbon Steel and Q450 Weathering Steel in Turpan,China

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Journal of Iron and Steel Research International 2016年第10期23卷 1061-1070页

作者： Qiang YU Chao-fang DONG Yue-hua fang Kui XIAO Chun-yun guo gang HE Xiao-gang LI Corrosion and Protection Center Key Laboratory for Corrosion and Protection University of Science and Technology Beijing Beijing 100083 China Xinjiang Turpan Natural Environmental Test Research Center Turpan 838200 Xinjiang China

Q235carbon steel and Q450 weathering steel were exposed to the hot and dry environment of Turpan,China for three *** corrosion rates of both steels were calculated and *** morphologies of the rust layer products were observed by optical microscopy and scanning electron *** of the rust layers were performed by X-ray photoelectron spectroscopy,X-ray powder diffraction,and Raman spectroscopy,and analysis results indicate that the compositions of rust are main iron rich oxide such as FeOOH,Fe_3O_4,and Fe_2O_*** iron oxide layer content proportion was calculated through a semi-quantitative *** resistance elements（Cr,Ni,and Cu）enhanced the resistance properties of the Q450 weathering steel ***,the resistance elements increased the proportion of goethite crystals in the corroded rust layer.

关键词： atmospheric corrosion Q235 carbon steel Q450 weathering steel rust layer

Initiation of Acupoint Molecular Mechanisms for Manual Acupuncture Analgesia—Nuclear FactorκB Signaling Pathway

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Chinese Journal of Integrative Medicine 2021年第12期27卷 933-939页

作者： ZHANG Kuo ZHAO xue YANG Tao ZHANG Yan-fang YAN Ya-wen XU zhi-fang GONG Yi-nan LIU Yang-yang guo Yong-ming guo Yi Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjin(300072)China Research Center of Experimental Acupuncture Science Tianjin University of Traditional Chinese MedicineTianjin(301617)China Affiliated Hospital of Municipal Institute of Traditional Chinese Medicine of Changzhi City Shanxi Province(046000)China Sanhuai Road Community Health Service Center Tanggu StreetBinhai New AreaTianjin(300451)China School of Traditional Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin(301617)China

Objective To identify the prominent molecular signaling in acupoints and explore their roles in initiating the analgesia effect of manual acupuncture(MA).Method A three-step study was conducted,the experiment 1 was a genome-wide analysis of the tissue at acupoint Zusanli(ST 36),including 12 Wistar rats which were divided into control,control+MA1,and control+MA7 *** the experiment 2,the paw withdrawal latency(PWL),immunohistochemistry and Western blot analysis of phospho-nuclear factor kappa B(NFκB)p65(p-p65),phospho-NFκB p50(p-p50)at ST 36 were performed on rats of saline,saline+MA,and complete Freund’s adjuvant(CFA)+MA groups(n=6).In experiment 3,24 rats were divided into saline+DMSO,CFA+DMSO,CFA+DMSO+MA,and CFA+BAY 11–7082+MA groups,the PWL and immunofluorescence assay of NFκB p65 at ST 36 was ***(1)The gene:inhibitor of NFκB(Nfkbia),interleukin-1β(Il1b),interleukin-6(Il6),chemokine c-x-c motif ligand 1(Cxcl1),monocyte chemoattractant protein-1(MCP-1/Ccl2)expressions in the control+MA7 group were significantly increased(P<0.05 or P<0.01),and the expression of NFκB p65(Rela),NFκB p50(Nfkb1)were increased in the control+MA7 group(P<0.05).(2)CFA+MA groups showed increased PWL from day 1 to 7(P<0.01 ***),and the Western blot results were consistent with immunohistochemistry,the expression of NFκB p-p65 and NFκB p-p50 were significantly increased in the MA-related groups compared with control and CFA groups(P<0.05).(3)Compared with the CFA+DMSO+MA group,the PWL of the CFA+BAY 11–7082+MA group decreased significantly and continued until day 5 and 7(P<0.05 and P<0.01,respectively),and the NFκB p65 expression of CFA+BAY 11–7082+MA was significantly reduced compared with CFA+DMSO+MA(P<0.01).Conclusion Local NFκB signaling cascade in acupoint caused by MA is an important step in initiating the analgesic effect,which would provide new evidence for the initiation of MA-effect and improve the understanding of the scientific basis of acupuncture analgesia.

关键词： manual acupuncture acupoint analgesia nuclear factor kappa B

Microstructural refinement and enhanced mechanical properties of suction-cast NiTi-Al alloy for structural use

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Rare Metals 2022年第8期41卷 2739-2744页

作者： Li-jing Zheng Ling guo fang-fang Wang Ming-Zhen Ma Hu Zhang Key Laboratory of Aerospace Advanced Materials and Performance of Ministry of Education School of Materials Science and EngineeringBeihang UniversityBeijing100191China School of Materials Science and Engineering Yanshan UniversityQinhuangdao066004China

A significant refinement of the microstructure of structural NiTi alloy was achieved through a combination of Al addition and fabrication by suction *** is attributed to the change from a layer-by-layer to volume solidification mode with Al content increasing,which causes a change in grain shape from columnar to ***,when compared with conventional casting,suction casting optimizes the microstructure of the alloy by refining the grain size and ensuring a homogeneous distribution of Ti2 Ni and Ni_(2)TiAl *** high cooling rate of suction casting also delays the formation ofβ'-Ni_(2)TiAl ***,this fine microstructure and uniform distribution of intermetallic phases result in the ultimate compressive strength of up to 2463.7 MPa,representing an improvement of 370 MPa over conventionally cast NiTi-Al alloy.

关键词： NiTi-Al intermetallic compound Suction casting Microstructure Compressive strength

Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers

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Chinese Journal of Polymer Science 2024年第11期42卷 1699-1709,I0008页

作者： Xiao-Ya guo zhi-Yu Yang Hua-Pan fang Dan-Hua Zhou Xuan Pang Hua-Yu Tian xue-Si Chen Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei230026China State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen UniversityXiamen361005China Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM) Xiamen361005China

Polyethyleneimine(PEI),as a widely used polymer material in the field of gene delivery,has been extensively studied for modification and shielding to reduce its ***,research aimed at preparing degradable PEI is *** this work,the hydrogen peroxide(H_(2)O_(2))oxidation method was used to introduce degradable amide groups in the PEI and a series of oxidized PEI22k(oxPEI22k)with different degrees of oxidation were synthesized by regulating the dosage of H_(2)O_(2).The relationship between the oxidation degree of oxPEI22k and the gene transfection efficiency of oxPEI22k was studied in detail,confirming that the oxPEI22k with oxidation degrees of 16.7%and 28.6%achieved improved transfection efficiency compared to unmodified *** oxPEI22k also proved reduced cytotoxicity and improved ***,this strategy was extended to the synthesis of low-molecular-weight *** oxPEI1.8k with suitable oxidation degree also achieved improved transfection efficiency and reduced *** brief,this work provided high-efficiency and low-cytotoxicity degradable gene delivery carriers by regulating the oxidation degree of PEI,which was of great significance for promoting clinical applications of PEI.

关键词： Polyethyleneimine Gene delivery Gene transfection Degradable gene carriers

Phase composition, morphology and element contents of micro-arc oxidation ceramic coatings on Ti–6Al–4V alloy under different calcination conditions

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Rare Metals 2016年第11期35卷 836-840页

作者： guo-Dong Hao xue-long Hao Zu-fang Zhu Department of Chemistry Mudanjiang Normal University National Center of Analysis and Testing for Nonferrous Metals and Electronic Materials General Research Institute for Nonferrous Metals

Compound ceramic coatings with the main crystalline of Al_2TiO_5（in the as-prepared coating without treatment） were prepared in situ on the surface Ti-6Al-4V alloy by means of pulsed bipolar micro-arc oxidation in Na AlO_2 solution. For the purpose of studying the antioxidation properties of the samples, the coated samples treated in argon and the as-coated samples were calcined in air at 1000 °C. And the related characteristics were investigated by X-ray diffraction（XRD）, scanning electron microscopy（SEM） and X-ray fluorescence（XRF） spectroscopy, respectively. The results show that, when it was calcined in air for 1 h, Al_2TiO_5in the as-prepared coating decomposed and transformed into α-Al_2O_3 and rutile TiO_***, after almost 4 h in argon, Al_2TiO_5in the asprepared coating decomposed and the final coating surface contents are completely α-Al_2O_3, and those of the middle interface are mainly Al_2O_3 and Ti_2O_3. The morphologies of the coatings after calcination in argon and air are ***-temperature oxidation occurred violently in the alloy substrate without coatings. Furthermore, the weight gain curves of the as-prepared samples and the coated samples treated in argon both show a parabolic shape.

关键词： Micro-arc oxidation Ti-6AI-4V Argon High-temperature oxidation

Dry eye disease in systemic lupus erythematosus: a cross sectional study

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1255-1261页

作者： Sui-fang Kang Ying-xue Wang Qi-Chen Zhang zhi-Lun Wang guo-Ling Chen Department of Ophthalmology the Second Hospital of Shandong UniversityJinan 250033Shandong ProvinceChina Department of Rheumatology the Second Hospital of Shandong UniversityJinan 250033Shandong ProvinceChina

AIM:To explore ocular surface manifestations of dry eye disease(DED)and its influencing factors in systemic lupus erythematosus(SLE)***:Ophthalmological examinations were conducted in SLE patients(n=43)and controls(n=41),including Ocular Surface Disease Index(OSDI),objective scatter index(OSI),tear meniscus height(TMH),lipid layer thickness(LLT),non-invasive Keratograph tear breakup time(NIKBUT),corneal fluorescein score(CFS),Schirmer I *** was diagnosed according to the Tear Film and Ocular Surface Society Dry Eye Workshop II *** patients were further divided into DED group and non-DED group,the disease activity,clinical manifestations and laboratory investigations were compared between the two *** disease activity was evaluated by Systemic Lupus Erythematosus Disease Activity Index 2000(SLEDAI-2K).Receiver operative characteristic(ROC)curve and multiplefactor binary logistic regression were ***:SLE patients showed higher OSDI[9.1(2.8-15.9)vs 6.3(2.2-7.5),P=0.035],higher OSI[1.67(1.09-2.60)vs 0.96(0.87-1.60),P=0.001],higher CFS[1(0-2)vs 0(0-1),P=0.001],lower LLT[65(42-100)vs 100(79.5-100),P=0.010],and lower NIKBUT[8.03(4.02-9.73)vs 9.67(5.26-12.71),P=0.030]than *** 32.6%of SLE patients had DED,which was higher than 12.2%of healthy *** group showed higher SLEDAI-2K score[9.7±6.1 vs 5.4±3.4,P=0.025],higher anti-cardiolipin antibody(ACL)[8.7(3.5-13.2)vs 3.6(2.0-6.9),P=0.035],and higher proportion of patients with cutaneous eruption[42.9%vs 6.9%,P=0.015]than non-DED *** to multiple-factor binary logistic regression analysis,the SLEDAI-2K score(OR=1.194,P=0.041)and cutaneous eruption(OR=7.094,P=0.045)could be consider as risk factors for DED in SLE *** ROC curve of the combined factors including age,disease duration,SLEDAI-2K score,ACL,and cutaneous eruption was analyzed,with a sensitivity of 0.786,a specificity of 0.793,and an area under curve of ***:Ocular surface affec

关键词： autoimmune disease systemic lupus erythematosus dry eye disease

应用KANO模型优化门诊护理服务的效果研究

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中国护理管理 2025年第1期25卷 11-15页

作者：汤赫男牛雅薇郭放吉林大学第一医院门诊部长春市130021 吉林大学第一医院透析中心长春市130021 吉林大学第一医院护理部长春市130021

目的:分析KANO模型在门诊护理服务中的应用效果,为提升门诊护理服务质量提供参考。方法:采用便利抽样法,从2022年10月至2023年4月来我院门诊就诊的患者中,筛选1200例患者作为研究对象,按就诊顺序,以优化前600例患者作为对照组,给予常规... 详细信息

目的:分析KANO模型在门诊护理服务中的应用效果,为提升门诊护理服务质量提供参考。方法:采用便利抽样法,从2022年10月至2023年4月来我院门诊就诊的患者中,筛选1200例患者作为研究对象,按就诊顺序,以优化前600例患者作为对照组,给予常规门诊护理服务,优化后600例患者作为实验组,以KANO模型为指导优化门诊护理服务,比较两组护理服务效果。结果:实验组患者预约到院后平均等待时间明显短于对照组,疾病知识普及率、预检分诊正确率和对护理服务的满意度均高于对照组(P<0.05)。结论:以KANO模型为指导开展门诊护理服务,可以显著缩短患者的等待时间,提高预检分诊正确率,赢得更多患者的满意,极大提升护理服务水平。

关键词：门诊护理服务 KANO模型应用效果就医体验

Black Hole Mass and Optical Radiation Mechanism of the Tidal Disruption Event AT 2023clx

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Research in Astronomy and Astrophysics 2025年第1期25卷 334-345页

作者： Shiyan Zhong Xian Xu Xinlei Chen Helong guo Yuan fang guowang Du Xiangkun Liu Xiaowei Liu South-Western Institute for Astronomy Research Yunnan University

We present the optical light curves of the tidal disruption event AT 2023clx in the declining phase,observed with *** our light curve with the ASAS-SN and ATLAS data in the rising phase,and fitting th composite multi-band light curves with MOSFiT,we estimate black hole mass for AT 2023clx is between 105.67and 105.82M⊙.This event may be caused by either a full disruption of a 0.1 M⊙star,or a partial disruption of0.99 M⊙star,depending on the data adopted for the rising *** on those fit results and the non-detection o soft X-ray photons in the first 90 days,we propose that the observed optical radiation is powered by stream-stream *** speculate that the soft X-ray photons may gradually emerge in 100–600 days after the optical peak when the debris is fully circularized into a compact accretion disk.

关键词： (galaxies:) quasars: supermassive black holes galaxies: nuclei radiation mechanisms: thermal