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Analysis of F_1 hybrid and BC_1 monosomic alien addition line plants from Brassica oleracea × Sinapis alba by GISH

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Chinese Science Bulletin 2006年第23期51卷 2872-2877页

作者： WEI Wenhui ZHANG Sufeng LI Jun WANG Lijun CHEN Bo fang xiaoping WANG Zhuan LUO Lixia Institute of Oil Crops the Chinese Academy of Agricultural Sciences Wuhan 430062 China College of Life Sciences Xinyang Normal College Xinyang 464000 China

Sterile and semi-fertile F_1 plants were obtained by intergeneric sexual hybridization between paternal Brassica oleracea var. alboglabra (genome CC, 2n=18) and maternal Sinapis alba (genome SS, 2n=24), BC_1 plants were obtained by backcrossing between paternal B. oleracea and ma-ternal semi-fertile F_1 plants. Genomic in situ hybridization (GISH) combined with dual-colour fluorescence in situ hybridization (dcFISH) showed that sterile F_1 plants contained 21 chromosomes consist-ing of one B. oleracea chromosome set and one S. alba chromosome set, belonging to expected hybrids, and semi-fertile F_1 plants contained 30 chromosomes consisting of two B. oleracea chromosome sets and one S. alba chromosome set. It is obvious that the semi-fertile F_1 plants belong to unexpected hybrids. 1―3 trivalents were detected at meiotic metaphase I of semi-fertile F_1 pollen mother cells (PMCs). Different separation ratios of S chromosomes were detected at anaphase I. A monosomic alien addition line (MAAL) was identified by GISH-dcFISH from BC_1 plants; it contained 19 chromosomes consisting of 18 C chromosomes and 1 S chromosome. At meiotic metaphase I, 9 divalents from B. oleracea and one univalent from S. alba could be detected. Sometimes, one putative C-S trivalent could also be detected. The achievement of B. oleracea-S. alba monosomic alien addition lines lays a foundation for gene introgression, location and cloning.

关键词：染色体芸苔 GISH 生物化学

A Barefoot Doctor’s Manual as a“Medical Bible”:Medical Politics and Knowledge Transmission in China

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Chinese Annals of History of Science and Technology 2019年第2期3卷 166-194页

作者： fang xiaoping Nanyang Technological University 639818 Singapore

This paper examines the origin,compilation,and circulation of A Barefoot Doctor’s Manual(Chijiao yisheng shouce赤脚医生手册),exploring the relationship between medical politics and knowledge transmission in China,and its impact on the promotion of Chinese medicine across the *** doctors were a special group of rural medical practitioners active in a very special socio-political *** editions of barefoot doctor manuals and textbooks were published across China after the first publication of the Manual in *** publication of these manuals and textbooks became an indelible hallmark of the“Cultural Revolution”(1966–1976),when political publications *** Manual was not only a guide for barefoot doctors in their daily study and practice,but also a primary source of medical knowledge for ordinary *** the middle of the 1970s,the Manual was translated into many languages and published *** paper argues that the publication of A Barefoot Doctor’s Manual embodied a public-oriented mode of knowledge transmission that emerged and was adopted during a very specific era,and though it was eventually substituted by a mode of training embedded in the formal medical education system,it demonstrated the impact of politics on medicine and health in the context of resource scarcity and low *** in China’s geopolitical status,the West’s pursuit of alternative approaches to medicine and health,and the World Health Organization’s(WHO’s)concern over health universality and equity all contributed to the translation and circulation of the Manual,facilitating the dissemination of Chinese medicine *** paper thus presents empirical and theoretical contributions to research on the relationship between medical politics and knowledge transmission in China.

关键词： A Barefoot Doctor’s Manual medical politics knowledge transmission circulation Chinese medicine

Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes

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Journal of Genetics and Genomics 2010年第5期37卷 333-340页

作者： fang Kong Cailin Ge xiaoping fang Rod J. Snowdon Youping Wang College of Bioscience and Biotechnology Yangzhou University Yangzhou 225009 China Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 China Institute for Plant Breeding and Crop Science I Justus Liebig University Heinrich-Buff-Ring 26-32 Giessen 35392 Germany

The diploid species Brassica rapa （genome AA） and B. oleracea （genome CC） were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins （32.0%） and specific proteins （39.8%） of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.

关键词： Brassica rapa Brassica oleracea proteomics molecular marker

Suppressive effect of dexamethasone on the neutrophil expression of CD18 in rats with radiation induced brain edema

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Neural Regeneration Research 2006年第1期1卷 36-39页

作者： Laixing Wang Yibin fang xiaoping Zhou Xiaowu Hu Jianmin Liu Department of Neurosurgery Changhai Hospital Second Military Medical University of Chinese PLA Shanghai 200433China

BACKGROUND： Stereo-tactic radiation therapy （SRT） is widely used to treat intracranial diseases, but some patients suffered from radiation induced brain edema after SRT. Once radiation induced brain edema occurs, the treatment is quite difficult, and it always leads to a poor outcome. Dexamethasone has certain therapeutic effect on traumatic brain edema, but the biological mechanism is still unclear. OBJECTIVE ： To observe the effect of dexamethasone on the neutrophil expression of CD18.DESIGN ： A randomized control observation.SETTING： Changhai Hospital of the Second Military Medical University of Chinese PLA. MATERIALS ： The experiment was carried out in Changhai Hospital of the Second Military Medical University of Chinese PLA from January 1999 to December 1999. Twenty SD rats （male and female each in half） weighing （250±50） g were used. METHODS： Twenty SD rats were divided into four groups at random. ① Blank control group （n=5）： The rats were not treated without dexamethasone or irradiation;② Irradiation group （n=5）： The rats were given irradiation but no dexamethasone treatment; ③ Irradiation＋1 mg/kg dexamethasone group （n=5）; The rats were treated with irradiation and dexamethasone of 1 mg/kg; ④Irradiation＋5 mg/kg dexamethasone group （n=5）： The rats were treated with irradiation and dexamethasone of 5 mg/kg. The heads of the rats were irradiated with 10 MeV X-ray （30 Gy）, and brain tissue was removed after 2 weeks to observe the pathological changes. Blood samples were taken from the carotid artery, gradient centrifugation was used, and neutrophile layer was obtained, the level of neutrophile expression of CD18 mRNA and quantity of membrane proteins in blood were detected with Northern blot and flow cytometry respectively. MAIN OUTCOME MEASURES： ① Blood cell count; ② Pathological results; ③ level of neutrophile expression of CD18 mRNA and quantity of membrane proteins. RESULTS ： All the 20 SD rats were involved in the analysis of results without

关键词： Suppressive effect of dexamethasone on the neutrophil expression of CD18 in rats with radiation induced brain edema Figure CD

中国的霍乱流行:防疫应急机制与“紧急规训国家”(1961—1965)

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中共党史研究 2022年第1期 96-99页

作者： [澳]方小平澳大利亚莫纳什大学文学院

和世界上其他国家一样,在中国历史上,战争、叛乱、社会政治动荡以及贸易往来造成的流离失所和人口流动常常导致致命性的急性传染病暴发和流行。随着19世纪初期全球化进程的加速,帝国主义扩张、贸易、政治暴力、军事冲突和宗教朝圣带来... 详细信息

和世界上其他国家一样,在中国历史上,战争、叛乱、社会政治动荡以及贸易往来造成的流离失所和人口流动常常导致致命性的急性传染病暴发和流行。随着19世纪初期全球化进程的加速,帝国主义扩张、贸易、政治暴力、军事冲突和宗教朝圣带来的频繁的人类迁移和互动,史无前例地加速了疾病的传播,并使其蔓延至中国。

关键词：政治暴力霍乱流行军事冲突应急机制政治动荡人类迁移 19世纪初期致命性

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基于K-means聚类组合模型的公交线路客流短时预测

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华南理工大学学报（自然科学版） 2019年第4期47卷 83-89,113页

作者：陈维亚潘鑫方晓平中南大学交通运输工程学院

预测公交线路短时客流是实现公交动态调度的关键技术.文中通过分析客流特性,构建了基于K-means聚类算法的组合预测模型.首先利用K-means算法将短时客流数据按照时变特征的相似度划分为不同聚类,然后为每类客流数据分别建立最小二乘支持... 详细信息

预测公交线路短时客流是实现公交动态调度的关键技术.文中通过分析客流特性,构建了基于K-means聚类算法的组合预测模型.首先利用K-means算法将短时客流数据按照时变特征的相似度划分为不同聚类,然后为每类客流数据分别建立最小二乘支持向量机、BP神经网络、自回归滑动平均模型,并考虑天气因素的影响,用遗传算法优化模型参数,对比预测结果,从中选择每个聚类的最佳预测模型构成组合模型.最后以长沙市104路公交客流数据作为实例进行预测分析,结果显示:客流数据时变特征对模型具有选择性,K-means聚类组合模型能够更好地根据不同时段客流数据的时变特征进行分类,因而有利于提高预测绩效;考虑了天气因素的K-means聚类组合模型能进一步提高公交线路的短时预测绩效.

关键词：公交线路客流短时预测 K-means聚类算法组合预测模型

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多对多越库配送绿色车辆路径问题及算法研究

铁道科学与工程学报 2022年第8期19卷 2202-2210页

作者：周果季彬方晓平中南大学交通运输工程学院湖南长沙410075

面向越库配送网络中供应商与客户之间多对多供需匹配场景,研究多对多越库配送绿色车辆路径问题,旨在优化越库配送模式下车辆配送路线,有效降低企业配送成本。以车辆运营成本、碳排放成本以及油耗成本之和最小化为目标,构建多对多越库配... 详细信息

面向越库配送网络中供应商与客户之间多对多供需匹配场景,研究多对多越库配送绿色车辆路径问题,旨在优化越库配送模式下车辆配送路线,有效降低企业配送成本。以车辆运营成本、碳排放成本以及油耗成本之和最小化为目标,构建多对多越库配送绿色车辆路径问题的数学优化模型。针对供需关系、复杂耦合特征以及离散组合方案巨大的特点,采用自适应大邻域算法框架并设计移除算子和修复算子对该问题进行高效求解。通过不同问题特征的测试算例对模型和算法进行测试,分析供应商-客户节点分布情况、越库中心位置、供应商-客户供需比等特征对调度方案的影响。车辆路径方案和算法收敛曲线表明了模型的有效性,提出的算法能够高效、稳定地求解多对多越库配送绿色车辆路径问题。数值仿真结果显示,构建的模型平均总成本比以车辆运营成本最小为目标时减少2.87%,比以碳排放和油耗成本最小为目标时减少0.11%,表明该模型能够有效地兼顾车辆运营成本、碳排放成本以及油耗成本。此外,供应商-客户供需比为1时相对于供需比为2和3时总成本分别减少41%和170%,表明较小的供应商-客户供需比利于减少配送总成本;越库配送中心不同选址方案的结果表明,将越库中心建设于配送网络中心位置有利于节省配送总成本。

关键词：车辆路径问题越库配送自适应大邻域搜索油耗和碳排放

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基于TPB的低碳交通出行方式研究

铁道科学与工程学报 2019年第3期16卷 804-811页

作者：方晓平周倩然中南大学交通运输工程学院湖南长沙410075 中国铁路广州局集团公司广东广州511400

以计划行为理论(TPB)为基础构建低碳出行行为模型,利用结构方程模型分析影响出行者交通行为决策的各因素,并收集整理我国大中型城市出行者数据进行验证。研究结果表明:所有的TPB因子均为有效影响因素,引入预期后悔和行为习惯可以增加TP... 详细信息

以计划行为理论(TPB)为基础构建低碳出行行为模型,利用结构方程模型分析影响出行者交通行为决策的各因素,并收集整理我国大中型城市出行者数据进行验证。研究结果表明:所有的TPB因子均为有效影响因素,引入预期后悔和行为习惯可以增加TPB对出行行为的解释力,出行意向对出行行为的效应最大。最后,基于各因素的作用路径提出加大宣传力度、完善公交系统和健全政策法规等政策建议。

关键词：低碳出行计划行为理论社会心理因素结构方程模型

数据不完备下基于特征识别的公交客流短时预测

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华南理工大学学报（自然科学版） 2020年第4期48卷 114-122页

作者：方晓平林美陈维亚潘鑫中南大学交通运输工程学院∥轨道交通大数据湖南省重点实验室湖南长沙410075

识别公交客流特征是提高短时预测质量的关键。但由于设备故障、数据收集受限等原因,客流数据属性往往是不完备的,这给特征识别和客流预测带来了挑战。文中以缺少乘客属性数据的长沙市104路公交卡数据为样本,利用卡号与出行时间的关联性... 详细信息

识别公交客流特征是提高短时预测质量的关键。但由于设备故障、数据收集受限等原因,客流数据属性往往是不完备的,这给特征识别和客流预测带来了挑战。文中以缺少乘客属性数据的长沙市104路公交卡数据为样本,利用卡号与出行时间的关联性识别乘客出行频次,以此作为区分出行特征的变量,将客流总集划分为不同的特征子集,依据子集规模、方差确定变量最佳取值,推断客流特征。与直接预测客流总集相比,文中为每类子集建立季节性差分自回归滑动平均(SARIMA)模型分别进行预测,整合后得出的样本外平均绝对误差改善了36.11%,依据乘客出行特征建立的预测模型拟合度为0.95,可有效识别公交客流特征。

关键词：公交客流短时预测不完备数据出行特征 SARIMA模型