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Interkingdom signaling in plant-microbe interactions

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Science China(Life Sciences) 2017年第8期60卷 785-796页

作者： Jinhong Kan rongxiang fang Yantao Jia State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China National Plant Gene Research Center Beijing 100101China University of the Chinese Academy of Sciences Beijing 100049China

The widespread communications between prokaryotes and eukaryotes via signaling molecules are believed to affect gene expression in both *** the communication process,the contacted organisms produce and release small molecules that establish communication channels between two kingdoms—this procedure is known as interkingdom *** communications are widespread between pathogenic or beneficial bacteria and their host plants,with diversified outcomes depending on the specific chemical-triggered signaling *** the signals or language of this interkingdom communication and uncovering the underlying mechanisms are major current challenges in this *** is evident that diverse signaling molecules can be produced or derived from bacteria and plants,and researchers have sought to identify these signals and explore the mechanisms of the signaling *** results of such studies will lead to the development of strategies to improve plant disease resistance through controlling interkingdom signals,rather than directly killing the pathogenic ***,the identification of signals produced by beneficial bacteria will be useful for agricultural *** this review,we summarize the recent progress of cross-kingdom interactions between plant and bacteria,and how LuxR-family transcription factors in plant associated bacterial quorum sensing system are involved in the interkingdom signaling.

关键词： interkingdom signaling bacteria host plants signals

Manipulation of biotic signaling:a new theory for smarter pest control

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Science China(Life Sciences) 2017年第8期60卷 781-784页

作者： Wei Qian Xiaoya Chen rongxiang fang Le Kang State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences State Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyChinese Academy of Sciences State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of Sciences

Modem agricultural bio-technologies have significantly increased the efficiency and quality of crop production and contributed to the improvement of people＇s life in the world. Widespread utilization of fertilizers, herbicides, fun- gicides, pesticides, antibiotics, and plant growth regulators has been instrumental in securing crop yields to feed over seven billions of human beings in the earth. However, dur- ing the recent half century, extensive uses of pesticides and other chemicals, which traditionally target the essential physiological pathways of pathogenic microbes and pests, have proverbial, negative impacts on the environmental and human health. Therefore, the urgent demands for sustaina- ble agriculture rely on a better understanding of interactions between pants and other organisms and the underpinning molecular metabolisms.

关键词：控制理论生物信号害虫植物生长调节剂农业可持续发展操纵农业生物技术人类健康

Small G Protein as a Novel Component of the Rice Bmssinosteroid Signal Transduction

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Molecular Plant 2016年第9期9卷 1260-1271页

作者： Ge Zhang Xiaoguang Song Hongyan Guo Yao Wu Xiaoying Chen rongxiang fang State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Center for Plant Gene Research (Beijing) Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China These authors contributed equally to this article. Present address: State Key Laboratory of Plant Genomics and NationaT Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of Sciences Beijing 100101 China

Brassinosteroids （BRs） are a class of steroid hormones that are essential for plant growth and development. The BR signal transduction pathway in the dicot model plantArabidopsis is well established, but the components connecting the BR signaling steps in rice have not been fully explored. For example, how the BR signaling is fine-tuned in rice, especially at the BR receptor level, is largely unknown. Here we show that OsPRA2, a rice small G protein, plays a repressive role in the BR signaling pathway. Lamina inclination, coleoptile elongation, and root inhibition assays indicated that rice plants with suppressed expression of OsPRA2 were more sensitive to exogenously applied brassinolide than the wild-type plants. Conversety, rice overexpressing OsPRA2 was less sensitive to exogenous brassinolide. Further study uncovered that OsPRA2 inhibited the dephosphorylation of, and thus inactivated the transcription factor BRASSINAZOLE- RESISTANT 1 （OsBZR1）. More importantly, OsPRA2 was found to co-localize with and directly bind to rice BR receptor BRASSlNOSTEROID-INSENSITIVE 1 （OsBRI1） at the plasma membrane. Additionally, the in vitro assays showed that OsPRA2 inhibits its autophosphorylation. This OsPRA2-OsBRI1 interaction led to the dissociation of OsBRI1 from its co-receptor OsBAK1, and abolished OsBRIl-mediated phosphorylation of OsBAK1. Together, these results reveal a possible working mechanism of OsPRA2 as a novel negative regu- lator on OsBRI1 and OsBZR1 and extend the knowledge about the regulatory mechanism of rice BR signaling.

关键词： brassinosteroid small G protein plant hormone signal transduction

RoTFL1c of Rosa multiflora has a dual-function in suppressing reproductive growth and promoting vegetative growth of Arabidopsis

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Science China(Life Sciences) 2018年第12期61卷 1599-1601页

作者： fang Wang Lijuan Lian Yunfeng Liu Yuman Zhang rongxiang fang Qinglin Liu Department of Ornamental Horticulture and Landscape Architecture China Agriculture UniversityBeijing 100193China State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

Dear Editor, TFL1homologues play a key role in the flowering habit of *** analyze the function of the newly isolated TFL1 gene of Rosa multiflora(Chen et al.,2013),named RoTFLlc,we performed overexpression analyses in Arabidopsis *** compared with empty vector control and wild type Arabidopsis plants,RoTFLlc overexpressing transgenic plants exhibited strong phenotypes such as a clustered habit,an increased number of rosette leaves, late flowering or failure to flower.

关键词： RoTFL1c Rosa multiflora

SRWD1, a novel target gene of DELLA and WRKY proteins, participates in the development and immune response of rice (Oryza sativa L.)

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Science Bulletin 2017年第24期62卷 1639-1648页

作者： Chuanmiao Xie Ge Zhang Yuman Zhang Xiaoguang Song Hongyan Guo Xiaoying Chen rongxiang fang State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Center for Plant Gene Research (Beijing) Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101. China

SRWD1, a member of the WD40 protein subfamily, is induced by salt stress in rice and its homolog in barley can bind to GAMYB, implying that SRWD1 might be involved in plant defense against environmental stress and gibberellic acid(GA) signalings. In this study, we focused on the biological functions and regulation mechanisms of SRWD1 in rice. The results showed that SRWD1 expression was repressed by GA and induced by abscisic acid(ABA). Two WRKY-family transcription factors, Os WRKY45 and Os WRKY72, were found to regulate SRWD1 expression by directly binding to the W-box region in its promoter. Transient co-expression and yeast two-hybrid analyses showed that a DELLA protein strengthened the activation of Os WRKY45 and partly relieved the suppression of Os WRKY72 by binding to ***, both SRWD1-overexpressing transgenic plants and SRWD1-knockout mutants showed dwarf phenotypes and resistance to Xanthomonas oryzae.

关键词： Phytohormone Gibberellin(GA) Abscisic acid(ABA) Signal transduction DELLA protein WRKY family protein

Function of resveratrol derived from transgenic plant expressing resveratrol syn-thase gene

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Chinese Science Bulletin 2001年第13期46卷 1103-1107页

作者： La Ping Cai Wenqi Zhang Jiuchun Zhang Fengli fang rongxiang Chinese Acad Sci Inst Microbiol Lab Plant Biotechnol Beijing 100080 Peoples R China

Two genes from grapevine coding for resvera-trol synthase, named RS1 and RS2, were cloned by RT-PCR. An Escherichia coli expression vector was constructed by insertion of RS1 into pBV221. A specific protein with the same molecular weight (42 ku) as the resveratrol synthase was expressed and used to prepare the rabbit antiserum. A plant expression vector was constructed by inserting the RS1 gene into pBin438 downstream of the doubled CaMV 35S promoter and TMV-Ω fragment. PCR-positive transgenic tobacco plants were obtained after transformation with Agrobacterium tumefaclens LBA4404 harboring the plant expression vector. Southern blot analysis demonstrated that the foreign gene was integrated into the tobacco genome. The results of RT-PCR and Western blot indicated that the RS1 gene was transcribed and expressed. Formation of resveratrol in transgenic tobacco was further determined by thin-layer chromatography of silica gel and HPLC. Increased accumulation of human breast adenocarcinoma cells in G0 and

关键词： resveratrol synthase gene transgenic tobacco resvera- trol human breast adenocarcinoma cell.

Purification and Characterization of a New Ribosome Inactivating Protein from Cinchonaglycoside C-treated Tobacco Leaves

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Journal of Integrative Plant Biology 2007年第9期49卷 1327-1333页

作者： Yanmei Li Yantao Jia Zhongkai Zhang Xiaoying Chen Hongping He rongxiang fang Xiaojiang Hao State Key Laboratory of Photochemistry and Plant Resources in West China Kunming Institute of Botany the Chinese Academy of Sciences Kunming 650204 China State Key Laboratory of Plant Genomics Institute of Microbiology the Chinese Academy of Sciences Beijing 100080 China Laboratory of Plant Virology Yunnan Academy of Agricultural Sciences Kunming 650223 China The Graduate School of the Chinese Academy of Sciences Beijing 100049 China

A new ribosome-inactivating protein （RIP） with a molecular weight of 31 kDa induced by Cinchonaglycoside C （1） designated CIP31, was isolated from tobacco leaves. Analysis of this protein sequence indicated that it belongs to the RIP family and it was distinct from the other plant RIPs reported previously at its N-terminal amino acid sequence. CIP31 can directly impair synthesis of coat protein （CP） of tobacco mosaic virus （TMV）, which resulted in inhibition of TMV long distance movement and multiplication in tobacco plants at concentrations of ng/mL. Furthermore, no toxicity was shown to the growth and fertility of the plants. CIP31 was synthesized only in the presence of Cinchonaglycoside C （1） and was independent of the salicylic acid （SA） signal pathway. We provided evidence for the SA-independent biological induction of resistance.

关键词： antiviral protein Cinchonaglycoside C (1) coat protein medical plant ribosome inactivating protein salicylic acid tobacco mosaic virus.

RcSPL1-RcTAF15b regulates the flowering time of rose (Rosa chinensis)

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Horticulture Research 2023年第6期10卷 182-194页

作者： Rui Yu Zhiying Xiong Xinhui Zhu Panpan Feng Ziyi Hu rongxiang fang Yuman Zhang Qinglin Liu Department of Ornamental Horticulture College of HorticultureChina Agricultural UniversityBeijing 100193China National Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciencesand National Plant Gene Research CenterBeijing 100101China University of Chinese Academy of Sciences Beijing 101408China

Rose(Rosa chinensis),which is an economically valuable floral species worldwide,has three types,namely once-flowering(OF),occasional or re-blooming(OR),and recurrent or continuous flowering(CF).However,the mechanism underlying the effect of the age pathway on the duration of the CF or OF juvenile phase is largely *** this study,we observed that the RcSPL1 transcript levels were substantially upregulated during the floral development period in CF and OF ***,accumulation of RcSPL1 protein was controlled by *** ectopic expression of RcSPL1 in Arabidopsis thaliana accelerated the vegetative phase transition and ***,the transient overexpression of RcSPL1 in rose plants accelerated flowering,whereas silencing of RcSPL1 had the opposite ***,the transcription levels of floral meristem identity genes(APETALA1,FRUITFULL,and LEAFY)were significantly affected by the changes in RcSPL1 ***15b protein,which is an autonomous pathway protein,was revealed to interact with *** silencing and overexpression of RcTAF15b in rose plants led to delayed and accelerated flowering,***,the study findings imply that RcSPL1–RcTAF15b modulates the flowering time of rose plants.

关键词： TAF flowering floral

Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants

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Science China(Life Sciences) 2011年第1期54卷 82-89页

作者： YANG YanMei LI Xia YANG Hui QIAN Yuan ZHANG You fang rongxiang CHEN XiaoYing State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Plant Gene Research Center Beijing 100101 China Graduate University of Chinese Academy of Sciences Beijing 100039 China Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 China Laboratory of Virology Capital Institute of Pediatrics Beijing 100020 China

The human pathogen, group A rotavirus, is the most prevalent cause of acute infantile and pediatric gastroenteritis worldwide, especially in developing countries. There is an urgent demand for safer, more effective and cheaper vaccines against rotavirus infection. Plant-derived antigens may provide an exclusive way to produce economical subunit vaccines. Virus-like particles, constituting viral capsid proteins without viral nucleic acids, are considered a far safer candidate compared with live attenuated viral vaccines. In this study, the rotavirus capsid proteins VP2, VP6 and VP7 were co-expressed in transgenic tobacco plants, and their expression levels, formation of rotavirus-like particles (RV VLPs) and immunogenicity were extensively studied. Quantitative real-time RT-PCR and Western blot analysis revealed that the expression level of vp6 was the highest while vp7 was expressed at the lowest levels. The RV VLPs were purified from transgenic tobacco plants and analyzed by electron microscopy and Western blot. Results indicated that the plant-derived VP2, VP6 and VP7 proteins self-assembled into 2/6 or 2/6/7 RV VLPs with a diameter of 60-80 nm. When orally delivered into mice with cholera toxin as an adjuvant, the total soluble protein extracted from transgenic tobacco plants induced rotavirus-specific antibodies comparable with those of attenuated rotavirus vaccines, while VP 2/6/7 induced higher serum IgG and fecal IgA titers compared with VP 2/6.

关键词： rotavirus virus-like particles transgenic plant oral vaccine immunogenicity