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Down-regulated expression of atypical PKC-binding domain deleted asip isoforms in human hepatocellular carcinomas

cell Research 2001年第3期11卷 223-229页

作者： fang chang minc, yong hua xu laboratory of molecular and cellular oncology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200031, china laboratory of molecular and cellular oncology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Asip is a mammalian homologue of polarity protein Par-3 of Caenorhabditis elegans and Bazooka of Drosophila melanogaster. Asip/Par-3/Bazooka are PDZ-motif containing proteins that localize asymmetrically to the cell periphery and play a pivotal role in cell polarity and asymmetric cell division. In the present study, we have cloned human asip cDNA and its splicing variants by 5’-RACE and RT-PCR using candidate human EST clones which have a high homology to rat asip cDNA. The full-length cDNA of human asip encodes a 1,353 aa protein exhibiting 88% similarity to the rat one. Human asip is a single copy gene consisting of at least 26 exons and localizing in human chromosome 10, band p11.2, with some extraordinarily long introns. All exon/intron boundary nucleotides conform to the "gt-ag" rule. Three main transcripts were detected by Northern blot analysis, and at least five variants, from alternative splicing and polyadenylation, have been identified by RT-PCR and liver cDNA library screening. Exon 17b deleted asip mRNAs expressed ubiquitously in normal human tissues, including liver, on RT-PCR analysis. However, they were absent from most human liver cancer cell lines examined. More interestingly, the expression of exon 1 7b deleted variants was down regulated in 52.6% (10/19) clinic specimens of human hepatocellular carcinomas (HCCs), compared with the surrounding nontumorous liver tissues from the same patients. The presence of various splicing transcripts, the variation of their distribution among different tissues and cells, and their differential expressions in human HCCs suggest that human Asip isoforms may function in different context.

关键词： Alternative splicing and polyadenylation A sip/Par-3/Bazooka PDZ-motif protein Polarity protein hepatocellular carcinomas.

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Deregulated c-myc expression in quiescent CHO cellsinduces target gene transcription and subsequent apoptotic phenotype

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cell Research 1999年第4期9卷 305-314页

作者： fang chang MING CAN SHI yong hua xu(laboratory of molecular and cellular oncology, shanghai)(institute of cell biology, chinese academy of sciences,320 Yue Yang Road, shanghai 200031, china) laboratory of molecular and cellular oncology Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai China

INTRODUCTIONThec-mycproto--oncogene,thecellularhomologueofthegenebornbytheretrovirusMC29,isresponsibleforchickenleukemia[1],anditoriginallygotitsnamefortheabilitytocausemyelocytomatosisinsusceptibleanimalsandculturedc... 详细信息

关键词： c-myc CHO cell line apoptosis c-myctarget genes.

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Stress Protein GRP170 Localizes Down-stream of c-Myc and Has Oncogenic Potential

Stress Protein GRP170 Localizes Down-stream of c-Myc and Has...

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中国细胞生物学学会第八届会员代表大会暨学术大会

作者： chang Ming fang and yong hua xu (laboratory of molecular and cellular oncology, shanghai institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, 320 Yue-yang Road, shanghai, 200031, P.R. china)

cells up-regulate the expression of molecular chaperones, such as glucose/oxygen-regulated proteins (GRPs/ORPs) and heat-shock proteins (HSPs), when subjected to environmental stress. GRP170/ORP150 is a novel endoplasmic reticulum-associated protein in the HSP70 family, which was first purified for its capacity to promote cell survival under the condition of hypoxia. We here reported that hamster grp170 was highly expressed in CHO-c-mycER cells when c-MycER was activated by the synthetic steroid, 4-hydroxy-tamoxifen (OHT). Moreover, the expression of its human homologue orp150 increased in some of human hepaitocellular carcinomas in which elevated c-myc expression was frequently detected. Our data indicated put GRP170/ORP150 down-steam of c-Myc signal pathway. The forced expression of grp170 in CHO cells stimulated the anchorage-dependent and anchorage-independent growth, reduced the serum dependency of cell growth in vitro. Serum starvation experiments showed that the over-expression of grp170 rescued the CHO cells frohi serum free induced DNA degradation and apoptosis of cells. Furthermore, the grp170 highly expressed CHO cells showed an increased potential of tumorigenicity in nude mice. The results suggest that grp170/orp150 is a down-stream target gene of oncogene c-Myc playing an active role in oncogenic transformation.

关键词： Differential display c-myc glucose/oxygen-regulated protein (GRP/ORP)

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反义封闭NGAL基因表达对SHEEC食管癌细胞微丝骨架的影响

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生物化学与生物物理进展 2004年第5期31卷 409-415页

作者：林珏龙许丽艳李恩民蔡唯佳牛永东方昆阳熊华淇沈忠英曾毅汕头大学医学院中心实验室汕头515031 汕头大学医学院肿瘤病理研究室汕头515031 汕头大学医学院生物化学与分子生物学教研室汕头515031 中国预防医学科学院病毒学研究所北京100052

为了研究反义封闭NGAL基因表达对SHEEC食管癌细胞微丝骨架以及肿瘤细胞生物学行为的影响 ,以不同长度NGAL基因片段反义表达载体和硫代修饰反义寡核苷酸单链片段转染SHEEC食管癌细胞 ,通过G4 18筛选 ,建立一系列旨在封闭SHEEC食管癌细胞N... 详细信息

为了研究反义封闭NGAL基因表达对SHEEC食管癌细胞微丝骨架以及肿瘤细胞生物学行为的影响 ,以不同长度NGAL基因片段反义表达载体和硫代修饰反义寡核苷酸单链片段转染SHEEC食管癌细胞 ,通过G4 18筛选 ,建立一系列旨在封闭SHEEC食管癌细胞NGAL基因表达的亚细胞克隆 .在细胞内F 肌动蛋白 (F actin)及DNA荧光双标记基础上 ,通过流式细胞术、激光共聚焦显微镜扫描术等技术手段检测封闭反义NGAL基因表达后 ,SHEEC食管癌细胞中F actin和DNA含量、F actin形态结构以及肿瘤细胞生物学行为的变化特征 .结果显示 ,反义封闭NGAL基因表达后 ,SHEEC食管癌细胞F actin的含量明显降低 ,与永生化食管上皮细胞SHEE相近 ,但细胞分裂增殖指数未见明显变化 .表明反义封闭NGAL基因表达对SHEEC食管癌细胞的微丝骨架有明显影响 ,而对SHEEC食管癌细胞的分裂增殖影响不明显 .激光共聚焦显微镜扫描观测显示 ,反义封闭NGAL基因表达可使SHEEC食管癌细胞F actin分布均匀 ,F actin小体减少 ,细胞间连接重新建立 ,结构较紧密 ,主要形态结构特征与SHEE细胞趋于一致 .提示反义封闭NGAL基因表达可对SHEEC食管癌细胞的微丝骨架F actin产生明显影响 ,推测癌细胞的微丝骨架F

关键词：微丝骨架 F-肌动蛋白 NGAL基因 SHEEC细胞细胞周期

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Abstracts from the 15th International Myopia Conference

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Eye and Vision 2016年第1期3卷 211-221页

作者： Alexandra Benavente-Perez Ann Nour Tobin Ansel Kathleen Abarr Luying Yan Keisha Roden David Troilo Chanyi Lu Miaozhen Pan Min Zheng Jia Qu Xiangtian Zhou Christine F. Wildsoet Fan Lu Jie Chen Jinhua Bao Liang Hu Qinmei Wang Zibing Jin Frances Rucker Stephanie Britton Stephan Hanowsky Molly Spatcher Hui-Ying Kuo Ching-Hsiu Ke I-Hsin Kuo Chien-Chun Peng Han-Yin Sun Ian G. Morgan Jeremy A. Guggenheim Rupal L. Shah Cathy Williams Jinglei Yang Peter S. Reinach Sen Zhang Wenfeng Sun Bo Liu Fen Li Xiaoqing Li Aihua Zhao Tianlu Chen Wei Jia Jun Jiang Haoran Wu Kazuo Tsubota Hiroko Ozawa Hidemasa Torii Shigemasa Takamizawa Toshihide Kurihara Kazuno Negishi Klaus Graef Daniel Rathbun Frank Schaeffel Ladan Ghodsi William K. Stell Machelle T. Pardue Ranjay Chakraborty Han na Park Curran S. Sidhu P. Michael Iuvone Michael J Collins Nethrajeith Srinvasalu Sally A. McFadden Paul N. Baird Pablo Artal Pauline Cho SW Cheung Pei-chang Wu Quan V. Hoang Duk C. Lee Erica G. Landis Michael A. Bergen Curran Sidhu Samer Hattar Richard A. Stone Ravi Metlapally Ruiqin Li Qinglin xu Hong Zhong Chenglin Pan Weizhong Lan Xiaoning Li Ling Chen Zhikuan Yang Scott A. Read Seang-Mei Saw Shi-Jun Weng Xiao-hua Wu Kang-Wei Qian Yun-Yun Li Guo-Zhong xu Furong huang Xiong-Li Yang yong-Mei Zhong Earl L Smith, III Baskar Arumugam Li-fang Hung Lisa A. Ostrin Klaus Trier Monica Jong Brien A. Holden Thomas Chuen Lam Samantha Shan Bing Zuo Dennis Yan-yin Tse Jingfang Bian King-Kit Li Quan Liu Chi-ho To Timothy J. Gawne John T. Siegwart, Jr. Alexander H. Ward Thomas T. Norton Yan Zhang Yue Liu Carol Ho Eileen Phan Abraham Hang Emily Eng Christine Wildsoet Department of biological sciences SUNY College of Optometry New York NY 10036 USA School of Optometry and Ophthalmology and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang 325027 China Center for Eye Disease & Development School of Optometry & Vision Science Program University of California Berkeley Berkeley CA 94720-2020 USA Wenzhou Medical University Wenzhou Zhejiang 325027 China Biomedical Science and Disease New England College of Optom Boston MA USA School of Optometry Chung Shan Medical University Taichung City 402 Taiwan institute of Medicine Chung Shan Medical University Taichung City 402 Taiwan Division of biochemistry and molecular biology Research School of Biology Australian National University Canberra Australia State Key laboratory of Ophthalmology and Division of Preventive Ophthalmology Zhongshan Ophthalmic Center Sun Yat-Sen University Guangzhou China School of Optometry & Vision sciences Cardiff University Cardiff UK School of Social and Community Medicine University of Bristol Bristol UK School of and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang 325027 China State Key laboratory Cultivation Base and Key laboratory of Vision Science Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry Wenzhou Zhejiang 325027 People’s Republic of China shanghai Key laboratory of Diabetes Mellitus and Center for Translational Medicine Shanghai Jiao Tong University Affiliated Sixth People’s Hospital Shanghai 200233 China Eye Hospital of Wenzhou Medical University Wenzhou Zhejiang 325027 China Department of Ophthalmology Keio University School of Medicine Shinjuku-ku Tokyo 160-8582 Japan General Medicine Ichikawa Medical Center Urayasu City Chiba 279-0001 Japan Ophthalmic Research institute University of Tuebingen 72076 Tuebingen Germany Bernstein Center for Computational Neuroscience Tuebingen 72076 Tuebingen Germany Werner Reichardt Centre for Integrative Neuroscience Otfried-Mueller-Straße 25 Tueb

To assess the peripheral refractive changes that occur when eye growth decelerates in *** We measured peripheral refraction and on-axis vitreous chamber depth on a total of 53 marmosets;36 treated monocularly for 12 weeks 9 hrs/day(26 with-5 D contact lenses,10 with+5 D)and 17 untreated *** the 26 marmosets treated with-5 D,10 wore contact lenses 9 hrs/day without interruptions and were mea-sured at the end of 10 weeks of treatment(T10)and after 4 weeks of recovery(R14).Eight had both contact lenses removed for 30 mins twice/day(mid-morning and mid-afternoon)during the first four weeks of treatment(early interruption group)and eight had the lenses removed during the second four weeks of treatment(late interruption group).The two interruption groups were measured after 4,8 and 12 weeks of treatment only(T4,T8,T12).

关键词： removed treatment untreated

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