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Highly sensitive detection of melamine based on reversed phase liquid chromatography mass spectrometry

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Chinese Science Bulletin 2009年第5期54卷 732-737页

作者： WU QingQing fan kexin SHA Wei RUAN HongQiang ZENG Rong SHIEH ChiaHui Key Laboratory of Systems Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Shanghai KeyTag Bioscience Inc. Shanghai 200233 China

In this work, we developed a highly sensitive method to detect melamine based on reversed phase liquid chromatography mass spectrometry. A mass spectrometry compatible ion pair, heptafluorobu-tyric acid(HFBA), was used to separate melamine by reversed phase liquid chromatography prior to electrospray mass spectrometry. The incorporation of isotope internal standard and multiple reaction monitoring improved the accuracy and linearity of quantification. Based on this strategy, the method limit of quantification was 0.1 ng/g. The limits of quantification were 8 ng/g for liquid milk and 15 ng/g for dry milk powder. This method provided a reproducible and stable approach to sensitive detection and quantification of melamine.

关键词：反相流相色谱质谱分析法离子对多反应监测模式三聚氰胺

Understanding the mechanistic regulation of ferroptosis in cancer:the gene matters

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Journal of Genetics and Genomics 2022年第10期49卷 913-926页

作者： Min Gao kexin fan Yuhan Chen Guangjian Zhang Jing Chen Yilei Zhang The Institute of Molecular and Translational Medicine Department of Biochemistry and Molecular BiologySchool of Basic Medical SciencesXi'an Jiaotong University Health Science CenterXi'anShaanxi 710061China Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'anShaanxi 710061China Department of Obstetrics Xi'an New Chang'an Maternity HospitalXi'anShaanxi 710001China Shaanxi Stem Cell Engineering Application Research Center Shaanxi Jiuzhou Biomedical Science and Technology GroupXi'anShaanxi 710065China

Ferroptosis has emerged as a crucial regulated cell death involved in a variety of physiological processes or pathological diseases,such as tumor suppression.Though initially being found from anticancer drug screening and considered not essential as apoptosis for growth and development,numerous studies have demonstrated that ferroptosis is tightly regulated by key genetic pathways and/or genes,including several tumor suppressors and oncogenes.In this review,we introduce the basic concepts of ferroptosis,characterized by the features of non-apoptotic,iron-dependent,and overwhelmed accumulation of lipid peroxides,and the underlying regulated circuits are considered to be pro-ferroptotic pathways.Then,we discuss several established lipid peroxidation defending systems within cells,including SLC7A11/GPX4,FSP1/CoQ,GCH1/BH4,and mitochondria DHODH/CoQ,all of which serve as anti-ferroptotic pathways to prevent ferroptosis.Moreover,we provide a comprehensive summary of the genetic regulation of ferroptosis via targeting the above-mentioned pro-ferroptotic or anti-ferroptotic pathways.The regulation of proand anti-ferroptotic pathways gives rise to more specific responses to the tumor cells in a contextdependent manner,highlighting the unceasing study and deeper understanding of mechanistic regulation of ferroptosis for the purpose of applying ferroptosis induction in cancer therapy.

关键词： Ferroptosis Ferroptotic pathways Genetic regulation Tumor suppression Cancer therapy

红柳黄酮提取工艺优化及其抗氧化、抑菌活性初探

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化学与生物工程 2024年第2期41卷 54-60页

作者：李金芳陈婷玉付金环范可欣高祎婷新疆第二医学院新疆克拉玛依834000

以红柳黄酮得率为评价指标,通过单因素实验和响应面法对红柳黄酮的提取工艺进行了优化,通过测定红柳不同部位黄酮提取物对DPPH自由基的清除率评价了其抗氧化活性,并考察了其对金黄色葡萄球菌、大肠杆菌、白色念珠菌的抑菌活性。结果表明... 详细信息

以红柳黄酮得率为评价指标,通过单因素实验和响应面法对红柳黄酮的提取工艺进行了优化,通过测定红柳不同部位黄酮提取物对DPPH自由基的清除率评价了其抗氧化活性,并考察了其对金黄色葡萄球菌、大肠杆菌、白色念珠菌的抑菌活性。结果表明,红柳黄酮的最佳提取工艺为:提取温度79.7℃、乙醇体积分数71.0%、提取时间2.19 h,在此条件下,红柳黄酮得率为5.24 mg·g^(-1);红柳不同部位黄酮提取物对DPPH自由基均有较强的清除能力;红柳不同部位黄酮提取物对金黄色葡萄球菌、大肠杆菌和白色念珠菌均有一定的抑菌活性。

关键词：红柳黄酮响应面法抗氧化活性:抑菌活性

面向农作物种质资源智能化管控与应用的本体构建

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农业图书情报学报 2024年第3期36卷 92-107页

作者：范可昕鲜国建赵瑞雪黄永文孙坦中国农业科学院农业信息研究所北京100081 中国农业科学院北京100081 国家新闻出版署农业融合出版知识挖掘与知识服务重点实验室北京100081 农业农村部农业大数据重点实验室北京100081

[目的/意义]以“生物技术+人工智能+大数据信息技术”为特征的育种4.0对种质资源的数字化管控与智能利用提出了新需求。为满足智能背景下对知识服务形态多样化的支持需求,文章旨在提出一种有效知识化组织、深度语义关联的方法。[方法/过... 详细信息

[目的/意义]以“生物技术+人工智能+大数据信息技术”为特征的育种4.0对种质资源的数字化管控与智能利用提出了新需求。为满足智能背景下对知识服务形态多样化的支持需求,文章旨在提出一种有效知识化组织、深度语义关联的方法。[方法/过程]通过分析领域数据描述及组织现状,参考作物本体、达尔文核心,融合《农作物种质资源技术规范》和实例数据,构建了覆盖粮食、经济等五大类农作物的本体模型,定义表型、基因型等11个核心类、10个对象属性和56个数据?属性。[结果/结论]基于该本体模型,文章提出农作物种质资源知识图谱构建思路,以及知识图谱驱动的智能问答、知识计算等新型智能化知识服务场景设计展望,以期为计算育种工作提供更加准确和高效的支持,为新质生产力的创新提供参考。

关键词：种质资源本体模型知识图谱新质生产力计算育种

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Ancient dormant virus remnant ERVW-1 drives ferroptosis via degradation of GPX4 and SLC3A2 in schizophrenia

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Virologica Sinica 2024年第1期39卷 31-43页

作者： Dongyan Zhang Xiulin Wu Xing Xue Wenshi Li Ping Zhou Zhao Lv kexin Zhao fan Zhu State Key Laboratory of Virology and Department of Medical Microbiology School of Basic Medical SciencesWuhan UniversityWuhan430071China Hubei Province Key Laboratory of Allergy&Immunology Wuhan UniversityWuhan430071China

Human endogenous retroviruses(HERVs)are remnants of retroviral infections in human germline cells from millions of years ago.Among these,ERVW-1(also known as HERV-W-ENV,ERVWE1,or ENVW)encodes the envelope protein of the HERV-W family,which contributes to the pathophysiology of schizophrenia.Additionally,neuropathological studies have revealed cell death and disruption of iron homeostasis in the brains of individuals with schizophrenia.Here,our bioinformatics analysis showed that differentially expressed genes in the human prefrontal cortex RNA microarray dataset(GSE53987)were mainly related to ferroptosis and its associated pathways.Clinical data demonstrated significantly lower expression levels of ferroptosis-related genes,particularly Glutathione peroxidase 4(GPX4)and solute carrier family 3 member 2(SLC3A2),in schizophrenia patients compared to normal controls.Further in-depth analyses revealed a significant negative correlation between ERVW-1 expression and the levels of GPX4/SLC3A2 in schizophrenia.Studies indicated that ERVW-1 increased iron levels,malondialdehyde(MDA),and transferrin receptor protein 1(TFR1)expression while decreasing glutathione(GSH)levels and triggering the loss of mitochondrial membrane potential,suggesting that ERVW-1 can induce ferroptosis.Ongoing research has shown that ERVW-1 reduced the expression of GPX4 and SLC3A2 by inhibiting their promoter activities.Moreover,Ferrostatin-1(Fer-1),the ferroptosis inhibitor,reversed the iron accumulation and mitochondrial membrane potential loss,as well as restored the expressions of ferroptosis markers GSH,MDA,and TFR1 induced by ERVW-1.In conclusion,ERVW-1 could promote ferroptosis by downregulating the expression of GPX4 and SLC3A2,revealing a novel mechanism by which ERVW-1 contributes to neuronal cell death in schizophrenia.

关键词： ERVW-1 Glutathione peroxidase 4(GPX4) Solute carrier family 3 member 2(SLC3A2) Ferroptosis Schizophrenia

基于网络药理学和分子对接技术研究西河柳化学成分治疗ALD的作用机制

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广东化工 2024年第1期51卷 124-126,123页

作者：陈婷玉范可欣付金环马腾徐睿君郭昕玥高祎婷新疆第二医学院新疆克拉玛依834000

目的:通过网络药理学和分子对接技术分析西河柳中有效化学成分治疗酒精性肝病的作用机制。方法:通过TCMSP平台检索西河柳活性化学成分及作用靶点,并以口服利用度≥30%和类药性≥0.18为预设条件对其进行筛选;同时,使用GeneCards、DrugBan... 详细信息

目的:通过网络药理学和分子对接技术分析西河柳中有效化学成分治疗酒精性肝病的作用机制。方法:通过TCMSP平台检索西河柳活性化学成分及作用靶点,并以口服利用度≥30%和类药性≥0.18为预设条件对其进行筛选;同时,使用GeneCards、DrugBank等数据库,筛选ALD相关靶点。通过VennDiagram软件可以获得一个交集靶点(西河柳和ALD);通过疾病、中药活性成分、交集靶点形成数据网络,最终遴选出致病的核心靶点进行分子结构处理和分子对接;进行GO功能富集和KEGG通路注释分析;结果由R语言和Perl语言进行可视化。结果:筛选获得槲皮素、山奈酚、异鼠李素等西河柳的主要活性化学成分,西河柳与酒精性肝病交集靶点共151个,GO功能富集分析得105条,KEGG通路筛选得129条信号通路。结论:本研究初步揭示了西河柳可通过多化学成分、多靶点、多通路治疗ALD,为天然药用植物的开发和利用提供了理论参考。

关键词：西河柳网络药理学分子对接 ALD 作用机制

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基于微分博弈的政企合作数据开放策略研究

重庆邮电大学学报（社会科学版） 2023年第5期35卷 98-114页

作者：樊自甫樊可欣重庆邮电大学经济管理学院重庆400065 重庆邮电大学网络协同经济研究中心重庆400065

数据作为一种关键生产要素,其开放、流通和利用水平直接影响着数字经济的发展。然而,现阶段我国的融合应用水平不高,政府和企业的数据开放力度以及数据开放质量仍有待加强。采用微分博弈方法,考虑数据开放程度和数据开放质量两个要素,... 详细信息

数据作为一种关键生产要素,其开放、流通和利用水平直接影响着数字经济的发展。然而,现阶段我国的融合应用水平不高,政府和企业的数据开放力度以及数据开放质量仍有待加强。采用微分博弈方法,考虑数据开放程度和数据开放质量两个要素,构建以政府、企业为主体的Nash非合作博弈、Stackelberg主从博弈、协同合作博弈模型,根据HJB方程求解三种情形下的政企最优行为策略、最优数据开放程度、最优数据开放质量水平以及最优收益轨迹,并在时间一致性的条件下,探讨政企合作收益分配机制,最后对三种情形下的结论进行仿真分析。政府为企业分担成本可以促进社会数据开放程度及质量水平的提升,实现帕累托改善;且在协同合作情形下,数据开放程度、数据开放质量水平及政企总收益最高,可实现系统效益最大化。文章建立了科学有效的成本分担机制,为政企合作开放数据提供指导,并制定了长期稳定的收益分配机制,为政企协同合作提供基础。

关键词：数据开放程度数据质量水平政企合作微分博弈动态收益分配

Will the Globe Encounter the Warmest Winter after the Hottest Summer in 2023?

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Advances in Atmospheric Sciences 2024年第4期41卷 581-586页

作者： Fei ZHENG Shuai HU Jiehua MA Lin WANG kexin LI Bo WU Qing BAO Jingbei PENG Chaofan LI Haifeng ZONG Yao YAO Baoqiang TIAN Hong CHEN Xianmei LANG fangxing fan Xiao DONG Yanling ZHAN Tao ZHU Tianjun ZHOU Jiang ZHU International Center for Climate and Environment Science(ICCES) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics(LASG) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Nansen-Zhu International Research Centre(NZC) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Center for Monsoon System Research(CMSR) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China CAS Key Lab of Regional Climate-Environment for Temperate East Asia(RCE-TEA) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China

In the boreal summer and autumn of 2023,the globe experienced an extremely hot period across both oceans and continents.The consecutive record-breaking mean surface temperature has caused many to speculate upon how the global temperature will evolve in the coming 2023/24 boreal winter.In this report,as shown in the multi-model ensemble mean(MME)prediction released by the Institute of Atmospheric Physics at the Chinese Academy of Sciences,a medium-to-strong eastern Pacific El Niño event will reach its mature phase in the following 2−3 months,which tends to excite an anomalous anticyclone over the western North Pacific and the Pacific-North American teleconnection,thus serving to modulate the winter climate in East Asia and North America.Despite some uncertainty due to unpredictable internal atmospheric variability,the global mean surface temperature(GMST)in the 2023/24 winter will likely be the warmest in recorded history as a consequence of both the El Niño event and the long-term global warming trend.Specifically,the middle and low latitudes of Eurasia are expected to experience an anomalously warm winter,and the surface air temperature anomaly in China will likely exceed 2.4 standard deviations above climatology and subsequently be recorded as the warmest winter since 1991.Moreover,the necessary early warnings are still reliable in the timely updated mediumterm numerical weather forecasts and sub-seasonal-to-seasonal prediction.

关键词： winter climate El Niño seasonal forecast GMST

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剥肋滚压复合工艺及其设备研究

机械工程学报 2024年第8期60卷 132-142页

作者：蒋飞赵升吨范淑琴王可心陈超西安交通大学机械工程学院西安710049 西安交通大学西安市智能装备与控制重点实验室西安710049 中南大学轻合金研究院长沙410083

面对我国对建筑钢筋的庞大需求以及钢筋两端高效精密制造出螺纹的要求,迫切需要新的加工工艺和设备来满足。分析各类螺纹滚压工艺原理、特点和工艺参数之间的关系,提出一种钢筋剥肋滚压复合新工艺。基于该工艺设计并研制了对称式剥肋滚... 详细信息

面对我国对建筑钢筋的庞大需求以及钢筋两端高效精密制造出螺纹的要求,迫切需要新的加工工艺和设备来满足。分析各类螺纹滚压工艺原理、特点和工艺参数之间的关系,提出一种钢筋剥肋滚压复合新工艺。基于该工艺设计并研制了对称式剥肋滚压设备,滚压模具相位保持机构和对称机构显著提高了钢筋端头螺纹成形的可靠性和效率。通过静力学分析了主轴变形以及箱体模态。利用有限元分析了滚压过程的塑性变形情况和材料流动规律,发现滚压过程中螺纹成形的等效应变随着模具的进给而增加,在切入段集中在齿廓周围,校正段和成形段均分布在整个螺纹,且坯料芯部没有应力和应变发生。牙侧材料从底部流向顶部导致齿高逐渐变高,且牙底的轴向位移最大。在所研制的设备上开展了剥肋滚压试验,验证了剥肋滚压工艺及其设备的可行性与可靠性,结果表明适当增加钢筋的剥肋直径可消除齿顶拉尖现象,同时精确的模具中心距是保证成形螺纹尺寸精度的重要因素。

关键词：钢筋螺纹滚压滚压设备有限元塑性变形