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Microgels for Cell Delivery in Tissue Engineering and Regenerative Medicine

Nano-Micro Letters 2024年第10期16卷 576-617页

作者： Leyan Xuan Yingying Hou Lu Liang Jialin Wu kai fan Liming Lian Jianhua Qiu Yingling Miao Hossein Ravanbakhsh Mingen Xu Guosheng Tang Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology the NMPA and State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436People's Republic of China School of Automation Hangzhou Dianzi UniversityHangzhou 310018People's Republic of China Department of Biomedical Engineering The University of AkronAkronOH 44325USA Wallace H.Coulter Department of Biomedical Engineering Georgia Institute of TechnologyAtlantaGA 30332USA

Microgels prepared from natural or synthetic hydrogel materials have aroused extensive attention as multifunctional cells or drug carriers,that are promising for tissue engineering and regenerative *** can also be aggregated into microporous scaffolds,promoting cell infiltration and proliferation for tissue *** review gives an overview of recent developments in the fabrication techniques and applications of microgels.A series of conventional and novel strategies including emulsification,microfluidic,lithography,electrospray,centrifugation,gas-shearing,three-dimensional bioprinting,*** discussed in *** characteristics and applications of microgels and microgel-based scaffolds for cell culture and delivery are elaborated with an emphasis on the advantages of these carriers in cell ***,we expound on the ongoing and foreseeable applications and current limitations of microgels and their aggregate in the field of biomedical *** stimulating innovative ideas,the present review paves new avenues for expanding the application of microgels in cell delivery techniques.

关键词： Microgels Cell delivery Scaffolds 3D bioprinting Single-cell microgels

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Epidemiological feature analysis of SVEIR model with control strategy and variant evolution

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Infectious Disease Modelling 2024年第3期9卷 689-700页

作者： kaijing Chen Fengying Wei Xinyan Zhang Hao Jin Zuwen Wang Yue Zuo kai fan School of Mathematics and Statistics Fuzhou UniversityFuzhou350116FujianChina Key Laboratory of Operations Research and Control of Universities in Fujian Fuzhou UniversityFuzhou350116FujianChina Center for Applied Mathematics of Fujian Province Fuzhou UniversityFuzhou350116FujianChina Jinzhou Center for Disease Control and Prevention Jinzhou121000LiaoningChina

The complex interactions were performed among non-pharmaceutical interventions,vaccinations,and hosts for all epidemics in China's Mainland during the spread of ***,the small-scale epidemic in the city described by SVEIR model was less found in the current *** SVEIR model with control was established to analyze the dynamical and epidemiological features of two epidemics in Jinzhou City led by Omicron variants before and after Twenty *** this study,the total population(N)of Jinzhou City was divided into five compartments:the susceptible(S),the vaccinated(V),the exposed(E),the infected(I),and the recovered(R).By surveillance data and the SVEIR model,three methods(maximum likelihood method,exponential growth rate method,next generation matrix method)were governed to estimate basic reproduction number,and the results showed that an increasing tendency of basic reproduction number from Omicron BA.5.2 to Omicron ***,the effective reproduction number for two epidemics were investigated by surveillance data,and the results showed that Jinzhou wave 1 reached the peak on November 1 and was controlled 7 days later,and that Jinzhou wave 2 reached the peak on November 28 and was controlled 5 days ***,the impacts of non-pharmaceutical interventions(awareness delay,peak delay,control intensity)were discussed extensively,the variations of infection scales for Omicron variant and EG.5 variant were also ***,the investigations on peaks and infection scales for two epidemics in dynamic zero-COVID policy were operated by the SVEIR model with *** investigations on public medical requirements of Jinzhou City and Liaoning Province were analyzed by using SVEIR model without control,which provided a possible perspective on variant evolution in the future.

关键词： COVID-19 SVEIR model Twenty measures Control strategy Variant evolution

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Counting of alpha particle tracks on imaging plate based on a convolutional neural network

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Nuclear Science and Techniques 2023年第3期34卷 52-63页

作者： Feng-Di Qin Han-Yu Luo Zheng-Zhong He Ke-Jun Lu Chuan-Gao Wang Meng-Meng Wu Zhong-kai fan Jian Shan School of Nuclear Science and Technology Hunan Provincial Key Laboratory of RadonUniversity of South ChinaHengyang 421001China School of Computer Science University of South ChinaHengyang 421001China China Institute of Atomic Energy Beijing 102413China

Imaging plates are widely used to detect alpha particles to track information,and the number of alpha particle tracks is affected by the overlapping and fading effects of the track *** this study,an experiment and a simulation were used to calibrate the efficiency parameter of an imaging plate,which was used to calculate the *** were created by using grayscale,which trained the convolutional neural network to count the alpha *** results demonstrated that the trained convolutional neural network can evaluate the alpha track counts based on the source and background images with a wider linear range,which was unaffected by the overlapping *** alpha track counts were unaffected by the fading effect within 60 min,where the calibrated formula for the fading effect was analyzed for 132.7 *** detection efficiency of the trained convolutional neural network for inhomogeneous ^(241)Am sources(2π emission)was 0.6050±0.0399,whereas the efficiency curve of the photo-stimulated luminescence method was lower than that of the trained convolutional neural network.

关键词： Imaging plate Convolutional neural network Alpha tracks counting

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Epidural Spinal Cord Stimulation Promotes Motor Functional Recovery by Enhancing Oligodendrocyte Survival and Differentiation and by Protecting Myelin after Spinal Cord Injury in Rats

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Neuroscience Bulletin 2020年第4期36卷 372-384页

作者： Gang Li Zhong-kai fan Guang-Fei Gu Zhi-Qiang Jia Qiang-Qiang Zhang Jun-Yu Dai Shi-Sheng He Department of Orthopedics Shanghai Tenth People's HospitalTongji University School of MedicineShanghai 200072China Spinal Pain Research Institute Tongji University School of MedicineShanghai 200072China Department of Orthopaedics The First Affiliated HospitalJinzhou Medical UniversityJinzhou 121001China Department of Spinal Surgery The Second Affiliated HospitalHenan University of Science and TechnologyLuoyang 471003China

Epidural spinal cord stimulation (ESCS) markedly improves motor and sensory function after spinal cord injury (SCI), but the underlying mechanisms are ***, we investigated whether ESCS affects oligodendrocyte differentiation and its cellular and molecular mechanisms in rats with SCI. ESCS improved hindlimb motor function at 7 days, 14 days, 21 days, and 28 days after *** also significantly increased the myelinated area at 28days, and reduced the number of apoptotic cells in the spinal white matter at 7 days. SCI decreased the expression of 20,30-cyclic-nucleotide 30-phosphodiesterase (CNPase,an oligodendrocyte marker) at 7 days and that of myelin basic protein at 28 days. ESCS significantly upregulated these markers and increased the percentage of Sox2/CNPase/DAPI-positive cells (newly differentiated oligodendrocytes) at 7 days. Recombinant human bone morphogenetic protein 4 (rh BMP4) markedly downregulated these factors after ESCS. Furthermore, ESCS significantly decreased BMP4 and p-Smad1/5/9 expression after SCI,and rh BMP4 reduced this effect of ESCS. These findings indicate that ESCS enhances the survival and differentiation of oligodendrocytes, protects myelin, and promotes motor functional recovery by inhibiting the BMP4-Smad1/5/9 signaling pathway after SCI.

关键词： Spinal cord injury Epidural spinal cord stimulation Oligodendrocyte Differentiation Remyelination

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A New Watermarking Protocol against Conspiracy

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Wuhan University Journal of Natural Sciences 2006年第6期11卷 1671-1674页

作者： ZHANG Jun KOU Weidong fan kai CAO Xuefei State Key Laboratory of Integrated Service Networks Xidian University Xi＇an 710071 Shaanxi China

A trusted third party introduced in watermarking protocols would decrease the security and affect the implementation of the protocols. In this paper, a new watermarking protocol with an un-trusted third party （UTTP） was proposed. Based on the idea of all-or-nothing disclosure of secret （ANDOS）, all of the buyer, the seller and the third party didn＇t know the exact watermark, which was embedded in a digital content for tracing piracy. The proposed protocol provided mechanisms to trace piracy and protect customer＇s right. In addition, the problem that a seller colluded with UTTP to frame the buyer, namely, the conspiracy problem, could be avoided.

关键词： watermarking protocol electronic commerce security digital copyright protection conspiracy problem

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Antioxidant capacity of extract from Jiangtang Xiaozhi recipe in vitro

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Journal of Traditional Chinese Medicine 2020年第3期40卷 393-400页

作者： Song Wang Yuan Yuan Yu Naixin Gu Haike Zhou Yunfeng Chen Xigong Wang Shuaiyu fan kai Ge Zhengyan Jin Long Xu Yang College of Biology and Environmental Sciences Jishou UniversityJishou 416000China Research Center for Pharmacology and Toxicology Institute of Medicinal Plant Development(IMPLAD)Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of EducationBeijing 100193China IMPLAD Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Beijing Radiation Center Beijing 100875China College of veterinary medicine China Agricultural UniversityBeijing 100083China Key Laboratory of Plant Resources Conservation and Utilization College of Hunan ProvinceJishou UniversityJishou 416000China Experiment Research Center of Xiyuan Hospital China Academy of Chinese Medical SciencesBeijing 100091China

OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe(JXR) of in ***: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical(DPPH) radical scavenging ability, 2-2’-Azinobis-(3-ethylbenzthiazoline-6-sul phonate(ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid ***: DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals;reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability;β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin ***: JXR extract has significant antioxidant capacity in vitro.

关键词： Free radical scavengers Lipid peroxidation Antioxidant capacity Jiangtang Xiaozhi recipe

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Controllable design of Zn-Ni-P on g-C_3N_4 for efficient photocatalytic hydrogen production

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Chinese Journal of Catalysis 2019年第3期40卷 390-402页

作者： Yanbing Li Zhiliang Jin Lijun Zhang kai fan School of Chemistry and Chemical Engineering North Minzu University Yinchuan 750021 Ningxia China Key Laboratory for Chemical Engineering and Technology State Ethnic Affairs Commission North Minzu University Yinchuan 750021 Ningxia China

Synthesizing a stable and efficient photocatalyst has been the most important research goal up to now. Owing to the dominant performance of g-C3N4 (graphitized carbonitride), an ordered assemble of a composite photocatalyst, Zn-Ni-P@g-C3N4, was successfully designed and controllably prepared for highly efficient photocatalytic H2 evolution. The electron transport routes were successfully adjusted and the H2 evolution was greatly improved. The maximum amount of H2 evolved reached about 531.2 μmol for 5 h over Zn-Ni-P@g-C3N4 photocatalyst with a molar ratio of Zn to Ni of 1:3 under illumination of 5 W LED white light (wavelength 420 nm). The H2 evolution rate was 54.7 times higher than that over pure g-C3N4. Moreover, no obvious reduction in the photocatalytic activity was observed even after 4 cycles of H2 production for 5 h. This synergistically increased effect was confirmed through the results of characterizations such as XRD, TEM, SEM, XPS, N2 adsorption, UV-vis DRS, transient photocurrent, FT-IR, transient fluorescence, and Mott-Schottky studies. These studies showed that the Zn-Ni-P nanoparticles modified on g-C3N4 provide more active sites and improve the efficiency of photogenerated charge separation. In addition, the possible mechanism of photocatalytic H2 production is proposed.

关键词： Zn-Ni-P g-C3N4 H2 production Controllable design Photocatalyst

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隐藏访问策略的高效CP-ABE方案

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计算机研究与发展 2019年第10期56卷 2151-2159页

作者：王悦樊凯西安文理学院信息工程学院西安710065 西安电子科技大学网络与信息安全学院西安710071

人工智能的发展离不开云计算的支撑,同样,人工智能的安全与云上大数据的安全也是密切相关的.目前,基于密文策略的属性基加密(ciphertext policy attribute-based encryption,CP-ABE)被认为是实现云上数据细粒度访问控制最有效的方法之一... 详细信息

人工智能的发展离不开云计算的支撑,同样,人工智能的安全与云上大数据的安全也是密切相关的.目前,基于密文策略的属性基加密(ciphertext policy attribute-based encryption,CP-ABE)被认为是实现云上数据细粒度访问控制最有效的方法之一.在基于密文策略属性基加密方案中,访问策略与密文相关且绑定,但很多时候,访问策略本身就是敏感信息,若以明文形式存放在云端会造成用户数据的泄露.因此,一种隐藏访问策略的高效CP-ABE方案被提出以解决这一问题.它可以使得属性隐藏和秘密共享能够同时应用到“与”门结构中,然后利用合数阶双线性群构造了一种基于包含正负及无关值的“与门”的策略隐藏方案,该方案有效地避免了用户的具体属性值泄露给其他第三方,确保了用户隐私的安全.此外,通过实验验证及分析,保证了该方案在实现复杂访问结构的策略隐藏的同时,还满足解密时间短,解密效率高的优点.

关键词：大数据安全属性加密访问结构策略隐藏访问控制

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物联网中超轻量级RFID电子票据安全认证方案

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计算机研究与发展 2018年第7期55卷 1432-1439页

作者：王悦樊凯西安文理学院信息工程学院西安710065 西安电子科技大学网络与信息安全学院西安710071

随着物联网应用技术的日益普及,作为其中重要技术之一的无线射频识别(radio frequency identification,RFID)技术在各个领域的应用场景越来越丰富.电子票据将RFID技术应用于传统票证,使得传统票证具备可存储、可识别、可验证等特性,在... 详细信息

随着物联网应用技术的日益普及,作为其中重要技术之一的无线射频识别(radio frequency identification,RFID)技术在各个领域的应用场景越来越丰富.电子票据将RFID技术应用于传统票证,使得传统票证具备可存储、可识别、可验证等特性,在很大程度上给人们的日常出行带来了巨大的便捷和高效.尽管如此,RFID系统在电子票证上的应用仍然面临着许多安全风险,比如隐私泄露.针对RFID技术在电子票据应用中存在的安全问题,提出了一种超轻量级的安全高效的认证方案.和一些采用复杂加密运算的方案相比,该方案采用简单的逻辑运算和时间戳同步升级机制,可有效抵抗失同步攻击和重放攻击,并可有效防止信息泄露.同时,该方案在数据库中采用时间戳匹配标签信息的方法,极大地提高了数据搜索效率.经过对安全性和高效性方面的分析以及对性能的仿真比较,可知所提方案相比现有方案在安全性和效率上均有较大提升.

关键词：无线射频识别安全认证超轻量级电子票据物联网

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核岛设计采购进度研究

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核动力工程 2006年第2期27卷 91-96页

作者：范凯徐顺中国广东核电工程有限公司广东深圳518124

用网络图的形式归纳了核岛设计采购基本逻辑框图,阐述了设计采购计划编排的基本依据和思路,按系统设计、施工设计及设备采购分类,给出了典型进度逻辑图,并分别对进度编排方案作了详细分析。

关键词：核岛设计采购进度控制

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