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Development and Implementation of a Rotating Nanoimprint Lithography Tool for Orthogonal Imprinting on Edges of Curved Surfaces

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Nanomanufacturing and Metrology 2021年第3期4卷 175-180页

作者： Shraddha Supreeti Ralf Schienbein Patrick feBer florian fern Martin hoffmann Stefan Sinzinger Electronics Technology Group Technical University of IlmenauIlmenauGermany Institute for Design and Precision Engineering Technical University of IlmenauIlmenauGermany Technical Optics Group Technical University of IlmenauIlmenauGermany JUMO GmbH&Co.KG FuldaGermany Microsystems Technology Group Ruhr-University BochumBochum.Germany

Uniform molding and demolding of structures on highly curved surfaces through conformal contact is a crucial yet often-overlooked aspect of nanoimprint lithography(NIL).This study describes the development of a NIL tool and its integration into a nanopositioning and nanomeasuring machine to achieve high-precision orthogonal molding and demolding for soft ultraviolet-assisted NIL(soft UV-NIL).The process was implemented primarily on the edges of highly curved plano-convex substrates to demonstrate structure uniformity on the ***-resolution nanostructures of sub-200-nm lateral dimension and microstructures in the range of tens of microns were ***,the nanostructures on the edges of the large,curved substrates were difficult to characterize ***,microstructures were used to measure the structure fidelity and were characterized using profilometry,white light interferometry,and confocal laser scanning *** of the restricted imaging capabilities at high inclinations for high-resolution nanostructures,the scanning electron microscope(SEM)imaging of the structures on top of the lens substrate and at an inclination of 45°was *** micro and nanostructures were successfully imprinted on the edges of the plano-convex lens at angles of 45°,60°,and 90°from the center of rotation of the rotating NIL *** method enables precise imprinting at high inclinations,thereby presenting a different approach to soft UV-NIL on curved surfaces.

关键词： Nanoimprint lithography Rotating NIL tool Curved surface Imprinting Nanopositioning

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Influence of the Heating Parameters on the Distortion of Quench Hardened AISI 52100 Steel Bearing Rings

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金属热处理 2004年第10期29卷 56-61页

作者： Kessler O Surm H hoffmann f Mayr P Stiftung Institut für Werkstofftechnik Bremen GermanyStiftung Institut für WerkstofftechnikBremen GermanyStiftung Institut für WerkstofftechnikBremen GermanyStiftung Institut für WerkstofftechnikBremen Germany

在协作研究中心 5 70“畸变工程”的“热处理过程中畸变协调加热”这一课题中 ,对加热过程中畸变发展机理进行了分析 ,并对影响热处理过程中畸变的奥氏体化参数进行了试验研究 ,并进行了计算机模拟。本文提出了不同加热参数对畸变的影... 详细信息

在协作研究中心 5 70“畸变工程”的“热处理过程中畸变协调加热”这一课题中 ,对加热过程中畸变发展机理进行了分析 ,并对影响热处理过程中畸变的奥氏体化参数进行了试验研究 ,并进行了计算机模拟。本文提出了不同加热参数对畸变的影响 ,并利用筛选法确定了其中的显著影响因素和它们对环状物 (AISI 5 2 10 0 ,EN 10 0Cr6轴承钢 )畸变的影响效果。装炉方式和加热速度对环的圆度及平面度的变化影响最大 ,加热过程中的奥氏体化温度。

关键词：淬火畸变热参数热处理过程奥氏体化温度加热轴承钢圆度影响效果 EN 环状

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Developing Exon-Primed Intron-Crossing （EPIC） markers for population genetic studies in three Aedes disease vectors

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Insect Science 2015年第3期22卷 409-423页

作者： Vanessa Linley White Nancy Margaret Endersby Janice Chan Ary Anthony hoffmann Andrew Raymond Weeks Department of Genetics Bio21 Institute the University of Melbourne Victoria 3010 Australia

Aedes aegypti, Aedes notoscriptus, and Aedes albopictus are important vectors of many arboviruses implicated in human disease such as dengue fever. Genetic markers applied across vector species can provide important information on population structure, gene flow, insecticide resistance, and taxonomy, however, robust microsatellite markers have proven difficult to develop in these species and mosquitoes generally. Here we consider the utility and transferability of 15 Ribosome protein （Rp） Exon-Primed Intron- Crossing （EPIC） markers for population genetic studies in these 3 Aedes species. Rp EPIC markers designed for Ae. aegypti also successfully amplified populations of the sister species, Ae. albopictus, as well as the distantly related species, Ae. notoscriptus. High SNP and good indel diversity in sequenced alleles plus support for amplification of the same regions across populations and species were additional benefits of these markers. These findings point to the general value of EPIC markers in mosquito population studies.

关键词： Aedes aegypti Aedes albopictus Aedes notoscriptus Exon-Primed Intron- Crossing (EPIC) indels Ribosome protein (Rp) SNPs

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Multisensor Data fusion for High Quality Data Analysis and Processing in Measurement and Instrumentation

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Journal of Bionic Engineering 2007年第1期4卷 53-62页

作者： Yan-bo Huang Yu-bin Lan W. C. hoffmann R. E. Lacey USDA ARS Agricultural Engineer Areawide Pest Management Research Unit Texas 77845 USA Biological and A g ricultural Engineering Department Texas A ＆M University Texas 77843-2117 USA

Multisensor data fusion （MDf） is an emerging technology to fuse data from multiple sensors in order to make a more accurate estimation of the environment through measurement and detection. Applications of MDf cross a wide spectrum in military and civilian areas. With the rapid evolution of computers and the proliferation of micro-mechanical/electrical systems sensors, the utilization of MDf is being popularized in research and applications. This paper focuses on application of MDf for high quality data analysis and processing in measurement and instrumentation. A practical, general data fusion scheme was established on the basis of feature extraction and merge of data from multiple sensors. This scheme integrates artificial neural networks for high performance pattern recognition. A number of successful applications in areas of NDI （Non-Destructive Inspection） corrosion detection, food quality and safety characterization, and precision agriculture are described and discussed in order to motivate new applications in these or other areas. This paper gives an overall picture of using the MDf method to increase the accuracy of data analysis and processing in measurement and instrumentation in different areas of applications.

关键词： multisensor data fusion artificial neural networks NDI food quality and safety characterization precision agriculture

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Using a Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella Typhimurium in Chicken Carcass

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Journal of Bionic Engineering 2008年第3期5卷 239-246页

作者： Yu-bin Lan Shi-zhou Wang Yong-guang Yin W. Clint hoffmann Xian-zhe Zheng USDA-ARS College Station TX77845 USA Department of Environmental and Occupational Health Texas A＆M University College Station TX 77845 USA College of Biological and Agricultural Engineering Jilin University Changchun 130022 P. R. China College of Engineering Northeast Agricultural University Harbin 150030 P. R. China

Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodbome pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance （SPR） biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. A taste sensor like electronic tongue or biosensors was used to basically ＂taste＂ the object and differentiated one object from the other with different taste sensor signatures. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with dif- ferent antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody con- centrations and dilution series of the organism. The SPR biosensor showed promising to detect the existence of Salmonella Typhimurium at 1 x 106 CfU/ml. Initial results show that the SPR biosensor has the potential for its application in pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.

关键词： biosensor Salmonella Typhimurium food safety chicken carcass detection limitation Surface Plasmon Resonance

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Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood,bone marrow and peritoneal lavage of pancreatic carcinoma patients

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World Journal of Gastroenterology 2007年第2期13卷 257-263页

作者： Katrin hoffmann Christiane Kerner Wolfgang Wilfert Marc Mueller Joachim Thiery Johann Hauss Helmut Witzigmann Department of Visceral Transplantation Thoracic and Vascular Surgery University of Leipzig Germany Institute of Laboratory Medicine Clinical Chemistry and Molecular Diagnostics University of Leipzig Germany

AIM： To evaluate the diagnostic potential of cytokeratin-19 （CK-19） mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas. METHODS： Sixty-eight patients with pancreatic cancer （/7 = 37）, chronic pancreatitis （n = 16）, and non-pancreatic benign surgical diseases （/7 = 15, control group） were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS： CK-19 mRNA expression was increased in 24 （64%） blood samples and 11 （30%） of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 （40%） of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors （G2/G3）. Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancer patients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION： Disseminated tumor cells can be detected in patients with pancreatic ductal adenocarcinorna by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers.

关键词： Tumor cell dissemination Pancreatic cancer Cytokeratin-19

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Genetic variation and potential coinfection of Wolbachia among widespread Asian citrus psyllid (Diaphorina citri Kuwayama) populations

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Insect Science 2019年第4期26卷 671-682页

作者： Chia-Ching Chu Mark hoffmann W. Evan Braswell Kirsten S. Pelz-Stelinski Department of Entomology and Nematology University of Florida Gainesville Florida USA Citrus Research and Education Center University of Florida Lake Alfred Florida USA 3Department of Horticultural Sciences North Carolina State University Raleigh North Carolina USA Center for Plant Health Science and Technology USDA APHIS Edinburg Texas USA

Wolbachia can profoundly influence the survival, reproduction, and defenses of insect hosts. These interactions could potentially be harnessed for managing pests or insecttransmitted diseases. Diaphorina citri Kuwayama is a phloem-feeding pest capable of transmitting the putative causal agent of citrus greening, Candidatus Liberibacter asiaticus (CLas). Like many insects, D. citri is also infected with Wolbachia (wDi). Recent studies indicate that the relative abundance of wDi could be associated with the abundance of CLas, and that wDi may contribute to regulating expression of phage lytic cycle genes in CLas, suggesting the need for better understanding of wDi biology in general. This study investigated the genetic diversity of wDi among D. citri in populations spanning eleven countries and two U.S. territories. Six Wolbachia genes, wsp, ***, gatB, and hep A, were sequenced and compared across samples. Two prevalent wDi strains were identified across the samples, and screening of clone libraries revealed possible coinfection of wDi strains in specific populations. D. citri mitochondrial cytochrome oxidase subunit I gene (mtCOI) were more divergent between D. citri populations that were infected with different wDi strains or had different infection statuses (single infection vs. coinfection). While we could not eliminate the possibility that maternal transmission may contribute to such patterns, it is also possible that wDi may induce cytoplasmic incompatibility in their host. These fin dings should contribute to the understanding of wDi population ecology, which may facilitate manipulation of this endosymbiont for management of citrus greening disease worldwide.

关键词： bacteria genetic diversity Huanglongbing plant disease superinfection vertically transmitted endosymbionts

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Predicting the effectiveness of protected areas of Natura 2000 under climate change

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Ecological Processes 2019年第1期8卷 162-182页

作者： Mst.Umme Salma Nila Carl Beierkuhnlein Anja Jaeschke Samuel hoffmann Md Lokman Hossain Global Change Ecology International Elite Study ProgrammeUniversity of BayreuthUniversitätsstraße 3095447 BayreuthGermany Department of Biogeography University of BayreuthUniversitätsstraße 3095447 BayreuthGermany Department of Geography Hong Kong Baptist University15 Baptist University RoadKowloon TongHong Kong Department of Environmental Protection Technology German University Bangladesh1702 GazipurBangladesh.

Background:Protected areas(PAs)are aimed to hold the environmental conditions that facilitate species and ecosystems to *** can become climatically unsuitable and unable to sustain their current number of species under climate *** Natura 2000(N2K)is the largest coordinated conservation tool assigned to maintain the long-term survival of Europe’s most significant species and *** attempting to understand the effectiveness of PAs in the face of climate change scenarios,we tested two hypotheses:(1)PAs in the Alpine and the Boreal biogeographical regions will experience more newly emerged climate conditions(hotter and drier)compared to the climate representation of other biogeographical regions under future climate in Europe and(2)PAs in the Mediterranean and the Continental biogeographical regions will face more consistency in climate conditions due to less area of disappearing and novel climate in ***:Current climate data(1960–1990)and projections for 2050 and 2070 of PAs of N2K were extracted from WorldClim global climate *** components analysis(PCA)was performed to construct climate space for the PAs across the biogeographical regions based on 19 climatic variables assessed at 5-km *** 10.1 was used to map the location of the novel and disappearing ***:PAs in the Alpine region will experience more novel climate conditions in the future compared to other biogeographical *** future projections showed that 17.70%of the PAs in the Alpine region will experience novel climate by *** climate consistency was observed in the PAs in the Continental region compared to the other biogeographical *** results showed that about 176 km2 of the selected PAs in the Continental region will face new emerging climate,while about 110 km2 will disappear under RCP 8.5 *** prediction also revealed that in the Mediterranean region 08 PAs will experience novel climate and 786 km2 areas

关键词： Biodiversity conservation Biogeographical region Climate change Climate scenarios Natura 2000,Protected areas

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Exploring new avenues in high repetition rate table-top coherent extreme ultraviolet sources

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Light(Science & Applications) 2015年第1期4卷 231-236页

作者： Steffen Hadrich Manuel Krebs Armin hoffmann Arno Klenke Jan Rothhardt Jens Limpert Andreas Tunnermann friedrich-Schiller-Universitat Jena Institute of Applied PhysicsAbbe Center of PhotonicsAlbert-Einstein-Straße 1507745 JenaGermany Helmholtz-Institute Jena Frobelstieg 307743 JenaGermany fraunhofer Institute for Applied Optics and Precision Engineering Albert-Einstein-Straße 707745 JenaGermany

The process of high harmonic generation(HHG)enables the development of table-top sources of coherent extreme ultraviolet(XUV)*** these are now matured sources,they still mostly rely on bulk laser technology that limits the attainable repetition rate to the low kilohertz ***,many of the emerging applications of such light sources(e.g.,photoelectron spectroscopy and microscopy,coherent diffractive imaging,or frequency metrology in the XUV spectral region)require an increase in the repetition ***,these sources are operated with a multi-MHz repetition rate and deliver a high photon flux *** far,this regime has been solely addressed using passive enhancement cavities together with low energy and high repetition rate ***,a novel route with significantly reduced complexity(omitting the requirement of an external actively stabilized resonator)is demonstrated that achieves the previously mentioned demanding parameters.A krypton-filled Kagome photonic crystal fiber is used for efficient nonlinear compression of 9 μJ,250 fs pulses leading to,7 μJ,31 fs pulses at 10.7 MHz repetition *** compressed pulses are used for HHG in a gas *** attention is devoted to achieving phase-matched(transiently)generation yielding.10^(13) photons s^(-1)(.50 μW)at 27.7 *** new spatially coherent XUV source improved the photon flux by four orders of magnitude for direct multi-MHZ experiments,thus demonstrating the considerable potential of this source.

关键词： coherent extreme ultraviolet sources high average power high harmonic generation nonlinear compression ultrafast laser

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Characterization of the first Wolbachia from the genus Scaptodrosophila,a male-killer from the rainforest species ***

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Insect Science 2022年第5期29卷 1401-1413页

作者： Kelly M.Richardson Michele Schiffer Perran A.Ross Joshua A.Thia Ary A.hoffmann School of Biosciences Bio21 InstituteThe University of MelbourneVictoriaAustralia Daintree Rainforest Observatory James Cook UniversityCape TribulationQueenslandAustralia

The Scaptodrosophila genus represents a large group of drosophilids with a worldwide distribution and a predominance of species in Australia,but there is little information on the presence and impacts of Wolbachia endosymbionts in this *** we describe the first Wolbachia infection from this group,wClay isolated from Scaptodrosophila claytoni(van Klinken),a species from the east coast of *** infection is polymorphic in natural populations,occurring at a frequency of around 6%–10%.wClay causes male killing,producing female-biased lines;most lines showed 100%male killing,though in 1 line it wasf males unless the infection is removed by tetracycline *** is transmitted at a high fidelity(98.6%)through the maternal lineage and has been stable in 2 laboratory lines across 24 generations,suggesting it is likely to persist in *** infection has not been previously described but is closely related to the male-killing Wolbachia recently described from Drosophila pandora based on multilocus sequence typing and the wsp ***-killing Wolbachia are likely to be common in drosophilids but remain difficult to detect because the infections can often be at a low frequency.

关键词： male-killer multilocus sequence typing phenotypic effects Scaptodrosophila Wolbachia

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