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Low serum neutralizing anti-SARS-CoV-2 S antibody levels in mildly affected COVID-19 convalescent patients revealed by two different detection methods

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Cellular & Molecular Immunology 2021年第4期18卷 936-944页

作者： Berislav Bosnjak Saskia Catherina Stein Stefanie Willenzon Anne Katrin Cordes Wolfram Puppe Gunter Bernhardt Inga Ravens Christiane Ritter Christian R.Schultze-florey Nina Godecke Jorg Martens Hannah Kleine-Weber Markus hoffmann Anne Cossmann Mustafa Yilmaz Isabelle Pink Marius M.Hoeper Georg M.N.Behrens Stefan Pohlm ann Rainer Blasczyk Thomas f.Schulz Reinhold forster Institute of Immunology Hannover Medical SchoolHannoverGermany lnstitute of Virology Hannover Medical SchoolHannoverGermany Department of Hematology HemostasisOncology and Stem Cell TransplantationHannover Medical SchoolHannoverGermany Institute of Transfusion Medicine and Transplant Engineering Hannover Medical SchoolHannoverGermany German Primate Center-Leibniz Institute for Primate Research GottingenGermany faculty of Biology and Psychology University GottingenGottingenGermany Department of Rheum atology and Immunology Hannover Medical SchoolHannoverGermany Public Health Department HannoverGermany Department of Pneumology and German Centre of Lung Research(DZL) Hannover Medical SchoolHannoverGermany German Center for Infection Research(DZIf) partner site Hannover-BraunschweigHannoverGermany Cluster of Excellence RESIST(EXC 2155) Hannover Medical SchoolHannoverGermany

Neutralizing antibodies targeting the receptor-binding domain(RBD)of the SARS-CoV-2 spike(S)block severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)entry into cells via surface-expressed angiotensin-converting enzyme 2(ACE2).We used a surrogate virus neutralization test(sVNT)and SARS-CoV-2 S protein-pseudotyped vesicular stomatitis virus(VSV)vector-based neutralization assay(pVNT)to assess the degree to which serum antibodies from coronavirus disease 2019(COVID-19)convalescent patients interfere with the binding of SARS-CoV-2 S to *** tests revealed neutralizing anti-SARS-CoV-2 S antibodies in the sera of ~90% of mildly and 100% of severely affected COVID-19 convalescent ***,sVNT and pVNT results correlated strongly with each other and to the levels of anti-SARS-CoV-2 S1 IgG and IgA ***,levels of neutralizing antibodies correlated with the duration and severity of clinical symptoms but not with patient *** to pVNT,sVNT is less sophisticated and does not require any biosafety *** this assay is also much faster and cheaper,sVNT will not only be important for evaluating the prevalence of neutralizing antibodies in a population but also for identifying promising plasma donors for successful passive antibody therapy.

关键词： COVID-19 SARS-CoV-2 Neutralizing antibody Serum ELISA

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Self-assembled Structures in Excess and Salt-free CatanionicSurfactant Solutions

Self-assembled Structures in Excess and Salt-free Catanionic...

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中国化学会第十届胶体与界面化学会议

作者：郝京诚 H.hoffmann 山东大学胶体与界面化学国家教育部重点实验室 Physikalische Chemie I Universitt BayreuthBT-95440Germany

＜正＞ The stable self-assembled structures and the properties in cationic and anionic （catanionic） surfactant solutions were reviewed and summarized. Two aspects of the recent results are focused on, one is the catanionic surfactant systems containing the excess salts in aqueous solutions formed from the combination of the two compounds, another is the ’true’, salt-free catanionic surfactant systems having the acidic （H+） and basic （OH-） as the counterions

关键词： Cationic and anionic surfactants Self-assembled aggregates Discs Icosahedra Vesicles

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Technology transfer in biological control of weeds:results dictate the needs

Technology transfer in biological control of weeds:results d...

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International Conference:Technology Transfer in Biological Control:from Research to Practice

作者： J.H.hoffmann Zoology Department University of Cape TownRondebosch 7700South Africa

Some biological control of weeds programmes are remarkably successful and,once initiated,require no intervention to keep the weed under control. However,in most cases,only partial control of the target weed is achieved because the performance of the natural enemies is moderated by the attributes of the environments into which they *** performance of

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家禽遗传资源可持续利用的挑战与选择

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中国家禽 2005年第14期27卷 39-44页

作者：刘向萍 I.hoffmann

前言全球的家禽生产大体有两种类型:一种是由国际化高度整合的公司掌控的大规模商品生产,还有一种是一些在最不发达国家中提供了90%家禽产品的小规模家禽生产.规模化商品生产的驱动因素有如下几点:繁殖周期短、单位成本低、研究和投入... 详细信息

前言全球的家禽生产大体有两种类型:一种是由国际化高度整合的公司掌控的大规模商品生产,还有一种是一些在最不发达国家中提供了90%家禽产品的小规模家禽生产.规模化商品生产的驱动因素有如下几点:繁殖周期短、单位成本低、研究和投入的规模经济及生产的可控制性.私立研究的精力主要集中在能应用于市场并能得到投资回报的技术研发上.

关键词：家禽遗传资源可持续利用育种知识产权品种遗传多样性

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Multisensor Data fusion of Remotely Sensed Imagery and Ground Sampling for Insect Damage Mapping

Multisensor Data Fusion of Remotely Sensed Imagery and Groun...

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亚洲精细农业与计算机农业应用联合大会

作者： W. Clint hoffmann USDA-ARS 2771 F&B Road College Station TX 77845

Prescription maps for aerial application were developed using remote sensing and GPS/GIS technologies. This makes it possible to perform multisensor, multi-spectral, multitemporal and even multi-resolution data fusion utilizing GIS technique to produce high quality prescription maps. The images were taken through the MS 4100 camera system. Mobile CE Mapper, Sunscan canopy analyzer, crop height sensor, fieldspec hyperspectral system, ADC multispectral camera, and GreenSeeker Handheld Optical Sensor were used for ground truth findings. Cessna 206 was used for this study. The aerial images were analyzed using Erdas software. This research was focus on using multisensor data fusion of remotely sensed imagery and ground sampling for insect damage mapping.

关键词： Prescription maps remote sensing GPS/GIS multisensor data fusion

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粒细胞集落刺激因子抗感染及抑制一氧化氮的性质<英文>

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Acta Pharmacologica Sinica 1999年第8期 3-5页

作者： Georg hoffmann,Wolfgang SCHOBERSBERGER Department of Physiology I University of Bonn Nussalle 11 D-53115 Bonn Germany. Division for General & Surgical Intensive Care Medicine Clinic for Anesthesia and Intensive Care Medicine University of Innsbruck Anichstrasse 35 A-6020 Innsbruck Austria

A proposed scheme between the possibleinteractions of pro- and anti-inflammatory cytokines,NO and G-CSf during severe inflammation/infection ispresented. Taken together, these data indicate that G-CSf exhibits anti-inflammatory properties which mayprove to be beneficial in situations associated with anincreased activity of the cellular immune *** the suppressive effects of G-CSf on theproduction of pro-inflammatory mediators like "fNf-αand nitric oxide are most likely neither cell type nor

关键词：粒细胞集落刺激因子细胞因子类感染炎症脓毒症一氧化氮合酶

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A LIM Domain Protein from Tobacco Involved in Actin-Bundling and Histone Gene Transcription

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Molecular Plant 2013年第2期6卷 483-502页

作者： Danièle Moes Sabrina Gatti CEline hoffmann Monika Dieterle flora Moreau Katrin Neumann Marc Schumacher Marc Diederich Erwin Grill Wen-Hui Shen André Steinmetz Cldment Thomas Centre de Recherche Public-SantE 84 Val Fleuri L-1526 Luxembourg Luxembourg Laboratoire de Biologie Moleculaire et Cellulaire du Cancer HSpital Kirchberg 9 rue Edward Steichen L-2540 Luxembourg Luxembourg Lehrstuhl for Botanik Technische Universitat Munchen EmiI-Ramann-StraBe 4 D-85354 Freising Germany Jnstitut de Biologie Moleculaire des Plantes du CNRS 12 rue du Général Zimmer F-67084 Strasbourg Ceédex France

The two LIM domain-containing proteins from plants （LIMs） typically exhibit a dual cytoplasmic-nuclear dis-tribution, suggesting that, in addition to their previously described roles in actin cytoskeleton organization, they partici-pate in nuclear processes. Using a south-western blot-based screen aimed at identifying factors that bind to plant histone gene promoters, we isolated a positive clone containing the tobacco LIM protein WLIM2 （NtWLIM2） cDNA. Using both green fluorescent protein （GfP） fusion-and immunology-based strategies, we provide clear evidence that NtWLIM2 local-izes to the actin cytoskeleton, the nucleus, and the nucleolus. Interestingly, the disruption of the actin cytoskeleton by latrunculin B significantly increases NtWLIM2 nuclear fraction, pinpointing a possible novel cytoskeletal-nuclear crosstalk. Biochemical and electron microscopy experiments reveal the ability of NtWLIM2 to directly bind to actin filaments and to crosslink the latter into thick actin bundles. Electrophoretic mobility shift assays show that NtWLIM2 specifically binds to the conserved octameric cis-elements （Oct） of the Arabidopsis histone H4A748 gene promoter and that this binding largely relies on both LIM domains. Importantly, reporter-based experiments conducted in Arabidopsis and tobacco proto-plasts confirm the ability of NtWLIM2 to bind to and activate the H4A748 gene promoter in live cells. Expression studies indicate the constitutive presence of NtWLIM2 mRNA and NtWLIM2 protein during tobacco BY-2 cell proliferation and cell cycle progression, suggesting a role of NtWLIM2 in the activation of basal histone gene expression. Interestingly, both live cell and in vitro data support NtWLIM2 di/oligomerization. We propose that NtWLIM2 functions as an actin-stabilizing protein, which, upon cytoskeleton remodeling, shuttles to the nucleus in order to modify gene expression.

关键词： actin BY-2 cytoskeleton DNA-binding histone genes LIM Nicotiana tabacum promoter regulation trans-acting factors.

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非均相催化精馏过程的模拟计算

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化工学报 1989年第6期40卷 693-703页

作者：张瑞生韩英 U.hoffmann 华东化工学院克劳斯塔尔工业大学

提出了多组分非均相催化精馏过程的数学模型.该微分方程组的边值是未知的,但受全塔总物料衡算方程的约束,模型的求解是一个困难的边值问题.借助于多用标打靶法和Newton-Raphson迭代,本文提出了模型的有效解法.提出的算法亦适用于在填料... 详细信息

提出了多组分非均相催化精馏过程的数学模型.该微分方程组的边值是未知的,但受全塔总物料衡算方程的约束,模型的求解是一个困难的边值问题.借助于多用标打靶法和Newton-Raphson迭代,本文提出了模型的有效解法.提出的算法亦适用于在填料塔内进行的非催化反应精馏过程.计算结果与实验数据的一致性令人满意.

关键词：非均相催化精馏数学模型填料塔

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离子液体凝胶

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山东大学学报(理学版) 2019年第1期54卷 1-18页

作者：高娟王晓琳 hoffmann Heinz 郝京诚山东药品食品职业学院基础教学部山东农业大学化学与材料科学学院山东大学胶体与界面化学教育部重点实验室

离子液体凝胶是以离子液体为分散介质形成的凝胶,作为一种新型的混合材料,离子液体凝胶不仅保持了离子液体原有的性质,而且解决了离子液体外溢的问题,其在形状上较高的可塑性满足了人们对特殊材料的需求,同时拓展了离子液体的应用范围... 详细信息

离子液体凝胶是以离子液体为分散介质形成的凝胶,作为一种新型的混合材料,离子液体凝胶不仅保持了离子液体原有的性质,而且解决了离子液体外溢的问题,其在形状上较高的可塑性满足了人们对特殊材料的需求,同时拓展了离子液体的应用范围。离子液体凝胶的种类较多,大致可分为物理型和化学型两大类。离子凝胶的结构、特性和应用目前成为胶体与界面科学家研究热点内容之一,也是近年来软物质科学研究中的主要内容。通过综述近年来离子液体凝胶的构筑、凝胶结构和性能与应用研究取得的进展,为未来离子液体凝胶的构筑及应用提供了重要的理论指导。

关键词：表面活性剂聚集体离子液体凝胶响应性

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Conserved profiles of digestion by double restriction endonucleases in insect genomes facilitate the design of dd RAD

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Zoological Systematics 2018年第4期43卷 341-355页

作者： Bingyan Li Qiao Gao Lijun Cao Ary Anthony hoffmann Qiong Yang Jiaying Zhu Shujun Wei Key Laboratory of forest Disaster Warning and Control of Yunnan Province College of Forestry Southwest Forestry University Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences School of BioSciences Bio21 Institute The University of Melbourne

Double-digested Restriction Site Associated DNA Sequencing（ddRAD） through next-generation sequencing（NGS） generates large numbers of loci for characterizing genomewide variation among multiple samples using next-generation sequencing. Different combinations of restriction endonucleases（REs） may produce varying size distributions of digested fragments, which affect the number of genotyped loci. Understanding digestion profiles across different species will help in selecting REs for digestion in a particular organism. In this study, we use of genome sequences to compare the in silico digestion profile of 26 combinations of REs in 131 insect species with two simulation programs. The number of digested fragments in the 300-450 bp range increases linearly with the size of the genome. Different species and insect orders showed similar profiles when digested by different combinations of REs in silico, indicating the conservation of digestion by double enzymes in insect genomes. Combinations with Nla III or TaqαI usually produced higher number of fragments in the range 300-450 bp, while combinations with EcoRI or MluCI produced fewer fragments. The proportion of fragments with the same overhangs at the two ends of digested DNA was higher than those with different overhangs. The two four-base enzyme pairs produced more fragments in the 300-450 bp range than pairs of four-base ＋ six-base enzymes. Experimental digestion of three species from Hymenoptera, Lepidoptera and Thysanoptera showed profiles congruent with in silico expectations. Our results shed light on understanding the digestion profiles of insect genomes and provide guidance on selecting REs for ddRAD projects.

关键词： Double-digested RADseq in silico simulation insect genome optimal doubledigestion combination

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