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Addition of Urban Waste to Semiarid Degraded Soil:Long-term Effect

Pedosphere 2007年第5期17卷 557-567页

作者： f.bastida J.L.MORENO C.GARC■A T.HERNNDEZ Department of Soil and Water Conservation Centro de Edafologia y Biologia Aplicada del Segura (CEBAS-CSIC) Campus Universitario de Espinardo30100 EspinardoMurcia (Spain).

The addition of municipal solid wastes （MSW） is considered as a possible strategy for soil rehabilitation in southeast Spain. The objective of this study was to evaluate the long-term （17 years） effect of five doses of MSW addition on the microbiological, biochemical, and physical properties of semiarid soil. Increased values of several parameters that serve as indicators of general microbiological activity, such as, basal respiration, adenosine triphosphate （ATP） or dehydrogenase activity; microbial population size （microbial biomass C）, and extracellular hydrolase activity related to macronutrient cycles, such as, urease, 3-glucosidase, and N-a-benzoyl-L-argininamide protease, were observed in the amended soils. The highest MSW doses showed the highest values in these hydrolase activities. The incorporation of municipal waste resulted in a more dense development of the plant cover, 50% greater in higher doses than in the control treatment, which generated a substantial increase in several C fractions. Total organic carbon reached 12 g kg^-1 soil with the highest MSW doses, compared to 4.30 g kg^-1 soil in the control treatment. The physical properties of the soil were also improved, showing greater percentage of stable aggregates and water holding capacity. Positive correlation coefficients between C fractions and parameters related to microbial activity and aggregate stability were observed. Although these improvements were greater in the soils receiving the highest doses of organic amendment, the increases were not proportional to the amount added, demonstrating the existence of a threshold, above which an increase in the amount of organic matter added is not reflected in an increase in the soil＇s physical, biochemical, and microbiological properties. However, the addition of municipal solid wastes proved its suitability for improving soil quality, thereby indicating the potential of such an amendment, to prevent desertification in Mediterranean areas such as th

关键词： enzyme activities long-term effect microbial biomass C municipal solid waste respiration

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Tracing Changes in the Microbial Community of a Hydrocarbon-Polluted Soil by Culture-Dependent Proteomics

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Pedosphere 2010年第4期20卷 479-485页

作者： f.bastida C.NICOLS J.L.MORENO T.HERNNDEZ C.GARCIA Department of Soil and Water Conservation Centro de Edafologia y Biologia Aplicada del Segura(CEBAS-CSIC)Campus Universitario de Espinardo

Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contaminated with diesel oil, to follow changes in the dominant species of the microbial community in the hydrocarbon-polluted soil via proteomics. Analysis of the proteins extracted from enriched cultures growing in Luria-Bertani （LB） media showed a change in the microbial community. The majority of the proteins were related to gIycolysis pathways, structural or protein synthesis. The results showed a relative increase in the complexity of the soil microbial community with hydrocarbon contamination, especially after 15 days of incubation. Species such as Ralstonia solanacearum, Synechococcus elongatus and different Clostridium sp. were adapted to contamination, not appearing in the control soil, although Bacillus sp. dominated the growing in LB in any of the treatments. We conclude that the identification of microbial species in soil extracts by culture-dependent proteomics is able to partially explain the changes in the diversity of the soil microbial community in hydrocarbon polluted semi-arid soils, but this information is much more limited than that provided by molecular methods.

关键词： culture dependent hydrocarbon contamination microbial diversity proteomics semiarid soil

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Response of Soil Microbial Community to a High Dose of fresh Olive Mill Wastewater

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Pedosphere 2013年第3期23卷 281-289页

作者： J.L.MORENO f.bastida M.A.S NCHEZ-MONEDERO T.HERNNDEZ C.GARCíA Department of Soil and Water Conservation Centro de Edafología y Biología Aplicada del Segura-Consejo Superior de Investigaciones Científicas (CEBAS-CSIC) Campus Universitario de Espinardo

An incubation experiment was designed in order to determine the further microbiological response to an addition （500 m3 ha-1） of fresh olive mill wastewater （fOMWW） in a soil that has been frequently amended with uncontrolled doses of OMWW since the 1990s in an active disposal site （ADS soil）. To achieve this aim, the phospholipid fatty acid （PLfA） profiles, microbial biomass C （Cmic）, and dehydrogenase （DHA） and urease activities （URA） were monitored at the beginning （To）, 3 h （T1） and 97 d （Tf, i.e., the end） of incubation after fOMWW addition. After the fOMWW addition, an increase in the ratio of fungal to bacterial PLfAs was observed in ADS soil. Moreover, a relative increase of monounsaturated fatty acids （MUfAs） with respect to saturated fatty acids （SATfA） was found in the ADS soil. An increase of the Gram-positive to Gram-negative ratio was observed in this soil at the end of the incubation. While DHA and Cmic increased in the ADS soil after fOMWW addition, URA showed a decrease. fungi and Gram-positive bacterial biomass experienced an increase after addition of a high dose of fOMWW in laboratory conditions.

关键词： enzyme activity microbial activity microbial biomass phospholipid fatty acid (PLfA) biomarker

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Descriptive and Comparative Histology of the Urinary System of the Hematophagous Bats Desmodus rotundus and Diphylla ecaudata

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Open Journal of Veterinary Medicine 2024年第7期14卷 180-191页

作者： María Guadalupe Ramírez-Muñoz Samantha Jardon-Xicotencatl José Ignacio Olave-Leyva Armando Zepeda-bastida Juan Ocampo-López Histology and Histopathology Laboratory Academic Area Veterinary Medicine and Zootechnics Academic Area Veterinary Biotechnology Institute of Agricultural Sciences Autonomous University of the Hidalgo State (UAEH) Tulancingo Mexico Multidisciplinary Research Unit L4 (Veterinary Morphology and Cell Biology) UNAM-FESC Campus 4 Cuautitln Izcalli Mexico Academic Area Veterinary Medicine and Zootechnics Institute of Agricultural Sciences Autonomous University of the Hidalgo State (UAEH) Tulancingo Mexico Cellular and Molecular Biology Laboratory Academic Area Veterinary Medicine and Zootechnics Institute of Agricultural Sciences Autonomous University of the Hidalgo State (UAEH) Tulancingo Mexico

Desmodus rotundus and Diphylla ecaudata, both of which are mammals of the order Chiroptera, Desmodontidae family, their diet consisting exclusively of blood. D. rotundus is the main vector and transmitter of the rabies virus, which affects human beings as well as several livestock species so the study of this bat species is of high importance within the fields of animal agriculture and public health. The present study describes and compares the histologic characteristics of the urinary system of two hematophagous bat species. A total of 5 bats from each species were captured in the municipalities of Progreso de Obregón, Hidalgo (D. rotundus), and Huayacocotla, Veracruz (D. ecaudata). Organs belonging to the urinary system were extracted: kidneys, ureters, urinary bladder, and urethra;samples were fixed using 10% formalin and processed by the paraffin embedding technique, obtaining sections of 5 µm thickness, which in turn were stained using hematoxylin-eosin (H-E) and Gomori trichrome (GT) stains. from the obtained histologic preparations, a descriptive and comparative analysis of the structural organography of the urinary system of both species was made, and no noteworthy histological differences between samples were noted. The present research is intended to provide a framework for future studies of these species’ currently understudied microscopic anatomy.

关键词： Microscopic Anatomy Vampire Bats Kidneys Comparative Morphology

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Ulcerative colitis in smokers,non-smokers and ex-smokers

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World Journal of Gastroenterology 2011年第22期17卷 2740-2747,2747页

作者： Guillermo bastida Belén Beltrán Department of Gastroenterology Hospital Universitari i Politecnic Centro de Investigaciones Biomedicas en Red en Enfermedades Hepáticas y Digestivas 46009 Valencia Spain

Smoking is a major environmental factor that interferes in the establishment and clinical course of ulcerative colitis (UC). firstly, the risk of smoking status impact in the development of UC is reviewed, showing that current smoking has a protective association with UC. Similarly, being a former smoker is associated with an increased risk of UC. The concept that smoking could have a role in determining the inflammatory bowel disease phenotype is also discussed. Gender may also be considered, as current smoking delays disease onset in men but not in women. No clear conclusions can be driven from the studies trying to clarify whether childhood passive smoking or prenatal smoke exposure have an influence on the development of UC, mainly due to methodology flaws. The influence of smoking on disease course is the second aspect analysed. Some studies show a disease course more benign in smokers that in non-smokers, with lower hospitalizations rates, less flare-ups, lower use of oral steroids and even less risk of proximal extension. This is not verified by some other studies. Similarly, the rate of colectomy does not seem to be determined by the smoking status of the patient. The third issue reviewed is the use of nicotine as a therapeutic agent. The place of nicotine in the treatment of UC is unclear, although it could be useful in selected cases, particularly in recent ex-smokers with moderate but refractory attacks of UC. finally, the effect of smoking cessation in UC patients is summarised. Given that smoking represents a major worldwide cause of death, for inpatients with UC the risks of smoking far outweigh any possible benefit. Thus, physicians should advise, encourage and assist UC patients who smoke to quit.

关键词： Smoking Ulcerative colitis Nicotine Inflammatory bowel disease Colectomy Pouchitis

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Abiotic and biotic drivers of struvite solubilization in contrasting soils

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Pedosphere 2023年第6期33卷 828-837页

作者： Antonio RUIZ-NAVARRO Manuel DELGADO-BAQUERIZO Concha CANO-DíAZ Carlos GARCíA felipe bastida Centro de Edafologia y Biologia Aplicada del Segura Consejo Superior de Investigaciones Cientificas(CEBAS-CSIC)Department of Soil and Water ConservationEspinardoMurcia E-30100Spain ICL Specialty fertilizers Iberia TotanaMurcia E-30850Spain Laboratorio de Biodiversidad y funcionamiento Ecosistemico Instituto de Recursos Naturales y Agrobiologia de Sevilla(IRNAS)CSICAv.Reina Mercedes 10Sevilla E-41012Spain IRNAS-CSIC Unidad Asociada Universidad Pablo de OlavideUniversidad Pablo de Olavide(UPO)Sevilla 41013Spain Centre for Research and Development in Agrifood Systems and Sustainability(CISAS) Instituto Politecnico de Viana do Castelo(IPVC)Rua Escola Industrial e Comercial Nun'Alvares 34Viana do Castelo 4900-347Portugal

Phosphorus(P) limitation in the coming decades calls for the utilization of alternative fertilizers in agriculture. Struvite is a promising P source, but its potential role as a fertilizer is dependent on different physical, chemical, and biological properties, which are very heterogeneous in soil, complicating the prediction of the best soil conditions for its application. Here, we evaluated the solubility of struvite in soil, its redistribution into P fractions, and its potential abiotic and biotic drivers in 62 globally distributed soils with contrasting properties through an incubation assay. We found that after 40 d, about 35% of struvite P was redistributed into soil fractions more accessible to plants and microbes. Phosphorus redistribution from struvite was driven by a complex suite of soil physical, chemical, and microbial properties as well as environmental factors that varied across soils. Soil texture played a critical role in determining the redistribution of P in struvite-amended soils in soluble(H2O extraction), labile(NaHCO3 extraction), and moderately labile(NaOH extraction) *** addition, the soil solution cation concentration was one of the most important drivers of available struvite-derived P fractions. The great importance of texture and cations in determining struvite-derived P fractions in soil was contrasted with the relatively minor role of pH. At the microbial level, the number of bacterial operational taxonomic units(OTUs) from the unfertilized soils that correlated with struvite-derived P fractions was higher than that of fungi. The number of OTUs that correlated with the struvite-derived soluble P fraction was dominated by fungi, whereas the number of OTUs that correlated with the struvite-derived labile P fraction was dominated by bacteria. Overall, this study provided a predictive framework for the potential use of struvite as a P fertilizer in contrasting soils.

关键词： bacteria fungi environmental factor operational taxonomic unit phosphorus fertilizer phosphorus fraction phosphorus solubilization soil fertility soil property

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Plant performance and morphofunctional differentiation in response to edaphic variation in Iberian columbines: cues for range distribution?

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Journal of Plant Ecology 2014年第4期7卷 403-412页

作者： Jesús M.bastida Pedro J.Rey Julio M.Alcántara Departamento de Biología Animal Biología Vegetal y EcologíaUniversidad de JaénParaje Las Lagunillas s/n.E-23071 JaénSpain

Aims Experimental studies of the response to environmental variation of closely related taxa are needed to understand the mechanisms underly-ing phenotypic divergence,habitat segregation and range span within a radiating *** explored the magnitude of phenotypic differenti-ation and adaptive plasticity in relation to edaphic variation and its sig-nificance for habitat segregation and range span in Iberian *** We performed a common garden experiment varying soil type(cal-careous ***)and depth(rocky-shallow ***)with two pairs of widespread and narrowly distributed subspecies of Iberian columbines:Aquilegia vulgaris(*** and nevadensis)and Aquilegia pyrenaica(*** and cazorlensis).We com-pared tolerance to edaphic variation,trait differentiation and adaptive plasticity of 10 morpho-functional traits between species and distri-bution ***,we obtained estimates of the competitive environment faced by each taxon in two to four populations per *** findingsresults partially support hypotheses of higher competitive ability and tolerance to edaphic variation in widespread than in narrow endemic *** the species level,the widely distributed taxon was the most tolerant to edaphic *** species,no consistent pattern emerged since the population from the most widespread subspecies was the most tolerant in *** but not in *** were differentiated in many traits at species and range ***,the pattern of differentiation does not fully support the hypothesis of higher specialization and stress toler-ance in narrow *** plasticity was generally low,the results support the hypothesis of adaptive plasticity in widespread but not in restricted taxa at least at species *** differences(adaptive plasticity and competitive ability among others)may have contributed to phenotypic divergence and edaphic niche segregation,as well as to differen

关键词： Aquilegia habitat differentiation phenotypic divergence phenotypic plasticity endemism

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Complex patterns of environmental niche evolution in Iberian columbines (genus Aquilegia, ranunculaceae)

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Journal of Plant Ecology 2015年第5期8卷 457-467页

作者： Rafael Jaime Julio M.Alcántara Jesús M.bastida Pedro J.Rey Departamento de Biología Animal Biología Vegetal y EcologíaUniversidad de JaénCampus Las Lagunillass/n.Jaén E-23071Spain

Aims This study explores the patterns of niche differentiation in a group of seven closely related columbines(genus Aquilegia,ranunculaceae)from the Iberian *** of these columbines are subject to complex patterns of divergent selection across environ-ments,which partly explain the taxonomic structure of the *** suggests the hypothesis that niche divergence must have occurred along the process of diversification of the *** We used maxEnt to build environmental niche models of seven subspecies belonging to the three species of Aquilegia present in the Iberian *** these models,we compared the envi-ronmental niches through two different approaches:ENmtools and multivariate *** findingsmaxEnt distributions conformed closely to the actual distribution of the study *** methods failed to uncover any clear patterns of niche differentiation or conservatism in Iberian *** analyses indicate the existence of dif-ferentiation along altitudinal gradients and along a gradient of climatic conditions determined by the summer precipitation and ***,climatic conditions related to winter tem-perature and precipitation,as well as soil properties,were equally likely to show conservatism or *** complex patterns of niche evolution we found suggest that Iberian Columbines have not been significantly constrained by forces of niche conservatism,so they could respond adaptively to the fast and profound climate changes in the Iberian Peninsula through the glacial cycles of the Pleistocene.

关键词： Aquilegia ecological niche modelling niche divergence niche conservatism evolution maxEnt ENmTools

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Enhancing Cloud-Based IoT/M2M System Scalability by Dynamic Network Slicing

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Communications and Network 2020年第3期12卷 122-154页

作者： David de la bastida fuchun Joseph Lin EECS International Graduate Program National Chiao Tung University Taiwan Department of Computer Science College of Computer Science National Chiao Tung University Taiwan

With ever-increasing applications of IoT, and due to the heterogeneous and bursty nature of these applications, scalability has become an important research issue in building cloud-based IoT/M2M systems. This research proposes a dynamic SDN-based network slicing mechanism to tackle the scalability problems caused by such heterogeneity and fluctuation of IoT application requirements. The proposed method can automatically create a network slice on-the-fly for each new type of IoT application and adjust the QoS characteristics of the slice dynamically according to the changing requirements of an IoT application. Validated with extensive experiments, the proposed mefont-family:Verdana;">chanism demonstrates better platform scalability when compared to a static slicing system.

关键词： Internet of Things Platform Virtualization Quality of Service Scalability Software Defined Networking

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Adalimumab in prevention of postoperative recurrence of Crohn's disease in high-risk patients

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World Journal of Gastroenterology 2012年第32期18卷 4391-4398页

作者： Mariam Aguas Guillermo bastida Elena Cerrillo Belén Beltrán Marisa Iborra Cristina Sánchez-Montes fernando Muoz Jesús Barrio Sabino Riestra Pilar Nos Gastroenterology Unit Department of Digestive DiseaseNetworked Biomedical Research Center for Hepatic and Digestive DiseasesLa Fe University and Politechnic Hospital46026 ValenciaSpain Department of Gastroenterology La Fe University and Politechnic Hospital46026 ValenciaSpain Leon Hospital Complex 42132 LeónSpain Río Hortega University Hospital 47001 ValladolidSpain Central University Hospital of Asturias 33006 OviedoSpain

AIM:To evaluate the effectiveness of adalimumab in preventing recurrence after intestinal resection for Crohn's disease in high-risk ***:A multicenter,prospective,observational study was conducted from June 2009 until June *** consecutively included high-risk Crohn's disease patients who had undergone an ileal/ileocolonic ***-risk patients were defined as two or more criteria:smokers,penetrating pattern,one or more previous surgical resections or prior extensive *** adalimumab was administered 2 wk(± 5 d) after surgery at a dose of 40 mg eow,with an initial induction dose of 160/80 mg at weeks 0 and *** data,previous and concomitant treatments(antibiotics,5-aminosalicylates,corticosteroids,immunomodulators or biologic therapies),smoking status at the time of diagnosis and after the index operation and number of previous resections(type and reason for surgery) were all *** status was assessed with C-reactive protein,erythrocyte sedimentation rate and fecal *** year(± 3 mo) after surgery,an ileocolonoscopy and/or magnetic resonance enterography was *** recurrence was defined as Rutgeerts score ≥ *** recurrence was based on magnetic resonance(MR) score ≥ ***:Twenty-nine patients(55.2% males,48.3% smokers at diagnosis and 13.8% after the index operation),mean age 42.3 years and mean duration of the disease 13.8 years were included in the study.A mean of 1.76(range:1-4) resections previous to adalimumab administration and in 37.9% was considered extensive resection.51.7% had previously received *** were given concomitantly to 17.2% of *** of the 29(13.7%) developed clinical recurrence,6/29(20.7%) endoscopic recurrence and 7/19(36.8%) morphological recurrence after *** patients with clinical recurrence showed endoscopic and morphological recurrence.A high degree of concordance was found between clinical-endosc

关键词： Crohn's disease Postoperative recurrence Prevention Tumor necrosis factor alpha agents Adalimumab

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