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A statistical approach to enhance the productivity of Streptomyces baarensis mH-133 for bioactive compounds

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Synthetic and Systems Biotechnology 2024年第2期9卷 196-208页

作者： mohamed H.Kalaba Gamal m.El-Sherbiny Osama m.Darwesh Saad A.moghannem Botany and microbiology Department Faculty of Science(Boys)Al-Azhar UniversityCairo 11884Egypt Agricultural microbiology Department National Research CentreDokkiCairoEgypt

The goal of this study was to use statistical optimization to change the nutritional and environmental conditions so that Streptomyces baarensis mH-133 could make more active *** trials were used to screen for critical variables influencing productivity using the Placket-Burman Design *** mH-133 is significantly influenced by elicitation,yeast extract,inoculum size,and incubation period in terms of antibacterial activity.A total of 27 experimental trials with various combinations of these factors were used to carry out the response surface technique using the Box-Behnken *** analyses revealed that the model was highly significant(pf-fit of 0.212 and a coefficient determination(R2)of ***,the model predicted that the response as inhibition zone diameter would reach a value of 27 *** optimal conditions,*** mH-133 produced 18.0 g of crude extract to each 35L and was purified with column *** active fraction exhibiting antibacterial activity was characterized using spectroscopic *** mIC and mBC values varied between 37.5 and 300μg/ml and 75 and 300μg/ml,*** conclusion,the biostatistical optimization of the active fraction critical variables,including environmental and nutritional conditions,enhances the production of bioactive molecules by Streptomyces species.

关键词： S.baarensis mH-133 Placket-Burman designBox-Behnken design ESKAPE Antibacterial activity

Impact of metal polluted sewage water on soil nematode assemblages in agricultural settings of Aligarh,India

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Soil Ecology Letters 2024年第1期6卷 61-75页

作者： mohammed f.S.A.Ghanem Shahid Afzal Humira Nesar Zarrin Imran Wasim Ahmad Nematode Biodiversity Research Lab Department of ZoologyAligarh Muslim UniversityAligarh 202002India

Sewage water in agriculture threatens human health and soil ecosystems through metal *** show promise as bioindicators of soil health due to their abundance and position in soil food ***-polluted water decreased abundance of certain nematode groups and Sigma maturity *** pollution positively affected nematode groups with r-selected life *** water has been inappropriately used in agriculture,posing possible threats to human health and the soil ecosystems by its constituent pollutants,especially heavy *** evaluation of its influences on soil biomes needs to consider the response of soil *** soil organisms,nematodes are seen as the best promising candidates for bioindicators of soil *** in,we collected soil samples from fresh water irrigated field from three sites(S1,S2 and S3)and sewage water irrigated distance gradient(5 m-40 m),to assess the influence of metal(Cu,Zn,Cd,mn,Pb)polluted water on various characteristics of nematode *** results indicated that the heavy metals decreased the abundance of C-p3 nematodes,herbivores,and predatory nematodes as well as sigma maturity index,whereas,C-p1,C-p2,bacterivore and fungivore nematodes abundance and diversity positively responded to the metal ***,nematode genera with r-selected life cycle were positively affected and those with K-selected life cycle were negatively affected by metal *** nematode community has potential to be used as indicator of pollution stress in agricultural soils to check soil health and sustainability.

关键词： heavy metals sewage irigation agriculture nematodes bioindicators

Green hydrogen energy production:current status and potential

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Clean Energy 2024年第2期8卷 1-7页

作者： Ali O.m.maka mubbashar mehmood The Libyan Centre for Research and Development of Saharian Communities MurzuqLibya School of Engineering and Physical Sciences Heriot-Watt UniversityEdinburghEH144ASUK

The technique of producing hydrogen by utilizing green and renewable energy sources is called green hydrogen ***,by implementing this technique,hydrogen will become a sustainable and clean energy source by lowering greenhouse gas emissions and reducing our reliance on fossil *** key benefit of producing green hydrogen by utilizing green energy is that no harmful pollutants or greenhouse gases are directly released throughout the ***,to guarantee all of the environmental advantages,it is crucial to consider the entire hydrogen supply chain,involving storage,transportation and end *** is a promising clean energy source and targets plan pathways towards decarbonization and net-zero emissions by *** paper has highlighted the techniques for generating green hydrogen that are needed for a clean environment and sustainable energy ***,it summarizes an overview,outlook and energy transient of green hydrogen ***,its perspective provides new insights and research directions in order to accelerate the development and identify the potential of green hydrogen production.

关键词： green hydrogen energy hydrogen production energy transition clean energy perspective of green hydrogen

On self-affine tiles that are homeomorphic to a ball

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Science China mathematics 2024年第1期67卷 45-76页

作者： Jorg m.Thuswaldner Shu-Qin Zhang mathematics and Statistics University of LeobenLeoben 8700Austria School of mathematics and Statistics Zhengzhou UniversityZhengzhou 450001China

Let m be a 3×3 integer matrix which is expanding in the sense that each of its eigenvalues is greater than 1 in modulus and let D?Z^(3)be a digit set containing|det m|*** the unique nonempty compact set T=T(m,D)defined by the set equation mT=T+D is called an integral self-affine tile if its interior is *** D is of the form D={0,v,...,(|det m|-1)v},we say that T has a collinear digit *** present paper is devoted to the topology of integral self-affine tiles with collinear digit *** particular,we prove that a large class of these tiles is homeomorphic to a closed 3-dimensional ***,we show that in this case,T carries a natural CW complex structure that is defined in terms of the intersections of T with its neighbors in the lattice tiling{T+z:z∈Z^(3)}induced by *** CW complex structure is isomorphic to the CW complex defined by the truncated octahedron.

关键词： self-afine sets tiles and tilings low-dimensional topology truncated octahedron

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Leveraging User-Generated Comments and fused BiLSTm models to Detect and Predict Issues with mobile Apps

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Computers, materials & Continua 2024年第4期79卷 735-759页

作者： Wael m.S.Yafooz Abdullah Alsaeedi Department of Computer Science College of Computer Science and EngineeringTaibah UniversityMedina42353Saudi Arabia

In the last decade, technical advancements and faster Internet speeds have also led to an increasing number ofmobile devices and users. Thus, all contributors to society, whether young or old members, can use these mobileapps. The use of these apps eases our daily lives, and all customers who need any type of service can accessit easily, comfortably, and efficiently through mobile apps. Particularly, Saudi Arabia greatly depends on digitalservices to assist people and visitors. Such mobile devices are used in organizing daily work schedules and services,particularly during two large occasions, Umrah and Hajj. However, pilgrims encounter mobile app issues such asslowness, conflict, unreliability, or user-unfriendliness. Pilgrims comment on these issues on mobile app platformsthrough reviews of their experiences with these digital services. Scholars have made several attempts to solve suchmobile issues by reporting bugs or non-functional requirements by utilizing user ***, solving suchissues is a great challenge, and the issues still exist. Therefore, this study aims to propose a hybrid deep learningmodel to classify and predict mobile app software issues encountered by millions of pilgrims during the Hajj andUmrah periods from the user perspective. firstly, a dataset was constructed using user-generated comments fromrelevant mobile apps using natural language processing methods, including information extraction, the annotationprocess, and pre-processing steps, considering a multi-class classification problem. Then, several experimentswere conducted using common machine learning classifiers, Artificial Neural Networks (ANN), Long Short-Termmemory (LSTm), and Convolutional Neural Network Long Short-Term memory (CNN-LSTm) architectures, toexamine the performance of the proposed model. Results show 96% in f1-score and accuracy, and the proposedmodel outperformed the mentioned models.

关键词： mobile apps issues play store user comments deep learning LSTm bidirectional LSTm

Numerical calculation of Z-scan measurements for nonlinear media with large phase shift

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Optoelectronics Letters 2024年第3期20卷 177-182页

作者： m.D.Zidan A.Allahham Department of Physics Atomic Energy Commission of SyriaP.O.Box 6091DamascusSyria

We have reported the characteristics of a Z-scan for the poly(azaneylylidene-acylene)(DAZA) polymer, as nonlinear medium with a large nonlinear phase shift using continuous-wave(CW) laser beam. It has been verified that the fresnel diffraction model is applicable for analyses of Z-scan measurements with DAZA polymer at high laser power. It was found that Z-scan curves with peak-to-valley features appear as the applied light intensity increases in the case of a large nonlinear phase shift. The Z-scan experiments were carried out using a CW laser to verify the theoretical calculations in the case of a large nonlinear phase shift model. Our results show good agreements between the experimental data and the proposed theoretical models.

关键词： nonlinear phase media

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Haplotype-based phylogenetic analysis and population genomics uncover the origin and domestication of sweetpotato

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molecular Plant 2024年第2期17卷 277-296页

作者： mengxiao Yan ming Li Yunze Wang Xinyi Wang m-Hossein moeinzadeh Dora G.Quispe-Huamanquispe Weijuan fan Yijie fang Yuqin Wang Haozhen Nie Zhangying Wang Aiko Tanaka Bettina Heider Jan f.Kreuze Godelieve Gheysen Hongxia Wang martin Vingron Ralph Bock Jun Yang Shanghai Key Laboratory of Plant functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai 201602China College of Life Sciences Chongqing Normal UniversityChongqing 401331China Biotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural SciencesChengdu 610061China College of Life Sciences Shanghai Normal UniversityShanghai 200234China Department of Computational molecular Biology Max Planck Institute for Molecular GeneticsIhnestraBe 63-7314195 BerlinGermany Department of Biotechnology Ghent University9000 GhentBelgium Guangdong Provincial Key Laboratory of Crops Genetics and Improvement Crop Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China Graduate School of Bioagricultural Sciences Nagoya UniversityChikusaNagoya 464-8601Japan international Potato Center(CIP) LimaPeru CAS Center for Excellence of molecular Plant Sciences Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200233China max-Planck-Institut fur molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany

The hexaploid sweetpotato(lpomoea batatas)is one of the most important root crops ***,its genetic origin remains controversial,and its domestication history remains *** this study,we used a range of genetic evidence and a newly developed haplotype-based phylogenetic analysis to identify two probable progenitors of *** diploid progenitor was likely closely related to lpomoea ae-quatoriensis and contributed the B,subgenome,IbT-DNA2,and the lineage 1 type of chloroplast genome to *** tetraploid progenitor of sweetpotato was most likely *** 4x,which donated the B2 subgenome,IbT-DNA1,and the lineage 2 type of chloroplast *** most likely originated from reciprocal crosses between the diploid and tetraploid progenitors,followed by a subsequent whole-genome *** addition,we detected biased gene exchanges between the subgenomes;the rate of B,to B2 subgenome conversions was nearly three times higher than that of B2 to B subgenome *** analyses revealed that genes involved in storage root formation,maintenance of genome stabil-ity,biotic resistance,sugar transport,and potassium uptake were selected during the speciation and domestication of *** study sheds light on the evolution of sweetpotato and paves the way forimprovementofthiscrop.

关键词： sweetpotato origin gene conversion domestication IbT-DNA HPA

methionine sources and genotype affect embryonic intestinal development, antioxidants, tight junctions, and growth-related gene expression in chickens

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Animal Nutrition 2024年第1期16卷 218-230页

作者： James K.Lugata Sawadi f.Ndunguru Gebrehaweria K.Reda Xénia E.Ozsváth Eszter Angyal Levente Czeglédi Gabriella Gulyás Renáta Knop János Oláh Zoltán mészár Rita Varga Brigitta Csernus Csaba Szabó Department of Animal Nutrition and Physiology Faculty of Agriculture and Food Sciences and Environmental Management University of Debrecen faculty of Agriculture and food Sciences and Environmental management Doctoral School of Animal Science University of Debrecen Department of Animal Husbandry Faculty of Agriculture and Food Sciences and Environmental Management University of Debrecen Department of Evolutionary Zoology and Human Biology Faculty of Science and Technology University of Debrecen Institutes for Agricultural Research and Educational farm University of Debrecen Department of Anatomy Histology and Embryology Faculty of Medicine University of Debrecen

methionine(met) is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and growth. The present study examined the effect of in ovo injection of DL-met and L-met sources and genotypes on chicken embryonic-intestinal development and health. fertilized eggs of the two genotypes, TETRA-SL layer hybrid(TSL)—commercial layer hybrid and Hungarian Partridge colored hen breed(HPC)—a native genotype, were randomly distributed into four treatments for each genotype. The treatment groups include the following: 1) control non-injected eggs(NoIn); 2) saline-injected(SaIn); 3) DL-met injected(DLm); and 4) L-met injected(Lm). The in ovo injection was carried out on 17.5 d of embryonic development; after hatching, eight chicks per group were sacrificed, and the jejunum was extracted for analysis. The results showed that both DLm and Lm groups had enhanced intestinal development as evidenced by increased villus width, villus height, and villus area(P mpared to the control. The DLm group had significantly reduced crypt depth, glutathione(GSH) content, glutathione S-transferase 3 alpha(GST3), occludin(OCLN) gene expression and increased villus height to crypt depth ratio in the TSL genotype than the Lm group(P factor 1(IGf1) gene, tricellulin(mD2), occludin(OCLN),superoxide dismutase 1(SOD1), and GST3 genes than the TSL genotype(P findings showed that in ovo injection of met enhanced intestinal development, and function, with genotypes responding differently under normal conditions. Genotypes also influenced the expression of intestinal antioxidants, tight junction, and growth-related genes.

关键词： DL-methionine L-methionine In ovo injection Intestinal development Poultry

Interfacial stress engineering toward enhancement of ferroelectricity in Al doped HfO_(2)thin films

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Chinese Physics B 2024年第9期33卷 637-643页

作者： S X Chen m m Chen Y Liu D W Cao G J Chen Department of microelectronics Jiangsu UniversityZhenjiang 212013China Department of Physics Jiangsu UniversityZhenjiang 212013China Guangdong–Hong Kong–macao Joint Laboratory for Intelligent micro-Nano Optoelectronic Technology Foshan UniversityFoshan 528225China

ferroelectric HfO_(2)has attracted much attention owing to its superior ferroelectricity at an ultra-thin thickness and good compatibility with Si-based complementary metal-oxide-semiconductor(CmOS)***,the crystallization of polar orthorhombic phase(o-phase)HfO_(2)is less competitive,which greatly limits the ferroelectricity of the as-obtained ferroelectric HfO_(2)thin ***,the crystallization of o-phase HfO_(2)can be thermodynamically modulated via interfacial stress *** this paper,the growth of improved ferroelectric Al doped HfO_(2)(HfO_(2):Al)thin films on(111)-oriented Si substrate has been *** analysis has suggested that nonpolar monoclinic HfO_(2):Al grown on(111)-oriented Si substrate suffered from a strong compressive strain,which promoted the crystallization of(111)-oriented o-phase HfO_(2)in the as-grown HfO_(2):Al thin *** addition,the in-plane lattice of(111)-oriented Si substrate matches well with that of(111)-oriented o-phase HfO_(2),which further thermally stabilizes the o-phase HfO_(2).Accordingly,an improved ferroelectricity with a remnant polarization(2P_(r))of 26.7C/cm^(2) has been *** results shown in this work provide a simple way toward the preparation of improved ferroelectric HfO_(2)thin films.

关键词： improved ferroelectricity interfacial stress engineering compressive strain HfO_(2)

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