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种子 1995年第2期14卷 62-63页

作者： A.juliano D.A.Vaughan ChuanYinWu f.j.zapata 吴关庭国际水稻研究所

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组织培养在水稻育种上的应用

福建稻麦科技 1988年第3期 44-49页

作者： f.j.zapata L.B.Torrizo 吴世雄

本文所讨论的组织培养技术包括花药培养、体细胞培养、离体胚培养、试管受精及原生质体的培养和融合。花药培养单倍体育种的组织培养方法包播花药/花粉培养、子房培养和染色体消减。本文主要讨论花药培养,它是上述三种培养方法中要简便... 详细信息

本文所讨论的组织培养技术包括花药培养、体细胞培养、离体胚培养、试管受精及原生质体的培养和融合。花药培养单倍体育种的组织培养方法包播花药/花粉培养、子房培养和染色体消减。本文主要讨论花药培养,它是上述三种培养方法中要简便、最有效和最可行的方法。虽然愈伤组织和绿苗再生率低的问题尚未完全解决,尤其是在籼稻上。但花培技术已日趋成熟,成为育种中必不可少的手段。

关键词：体细胞无性系植株亲本原生质体再生水稻育种花培野生稻诱发变异人工变异花药培养愈伤组织创伤组织

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籼稻体细胞胚胎发生的组织学及细胞学研究

Acta Botanica Sinica 1988年第5期30卷 485-489页

作者：凌定厚 D.S.Brar f.j.zapata 中国科学院华南植物研究所印度旁遮普邦农业大学国际水稻研究所

以成熟种子为外植体,在离体培养中研究了体细胞胚胎发生。组织学研究表明,愈伤组织是由成熟种子盾片的表皮吸收细胞首先产生的,随后从愈伤组织分化出胚状体。象水稻合子胚的结构一样,体细胞胚状体主要由盾片、胚芽稍及胚根组成。在胚状... 详细信息

以成熟种子为外植体,在离体培养中研究了体细胞胚胎发生。组织学研究表明,愈伤组织是由成熟种子盾片的表皮吸收细胞首先产生的,随后从愈伤组织分化出胚状体。象水稻合子胚的结构一样,体细胞胚状体主要由盾片、胚芽稍及胚根组成。在胚状体内可以观察到不同发育时期的原胚,包括大量的单一的胚性细胞、二细胞、四细胞及分化程度不同的原胚以及一些畸形胚状体。从本实验可以初步认为由籼稻体细胞培养而获得的胚状体在结构上也有象合子胚一样的基本结构,并且是单细胞起源的。

关键词：细胞学体细胞胚胎发生胚状体组织学单细胞籼稻离体培养

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运用植物毒素离体筛选水稻抗胡麻叶斑病种质的研究

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journal of Genetics and Genomics 1986年第3期 194-200页

作者：凌定厚,P.Vidhyaseharan,E.S.Borromeo,f.j.zapata,苗东华中国科学院华南植物研究所国际水稻研究所国际水稻研究所国际水稻研究所国际水稻研究所广州菲律宾马尼拉菲律宾马尼拉菲律宾马尼拉菲律宾马尼拉

以胡麻叶斑病病原菌的毒素配制的培养基,培养水稻的体细胞愈伤组织,在其再分化第一代（R1）植株群体中,发现3株(其中IR52株经25％毒素处理,IR541株未经毒素处理)抗胡麻叶斑病的突变。在这3个抗性系统的R2（R2植株的种子后代）群体中,均... 详细信息

以胡麻叶斑病病原菌的毒素配制的培养基,培养水稻的体细胞愈伤组织,在其再分化第一代（R1）植株群体中,发现3株(其中IR52株经25％毒素处理,IR541株未经毒素处理)抗胡麻叶斑病的突变。在这3个抗性系统的R2（R2植株的种子后代）群体中,均观察到对胡麻叶斑病病原菌的敏感与抗性的分离。本文是以植物毒素作为筛选因子,运用组织培养技术,在水稻上成功地筛选出抗病植株的首次报道。

关键词：胡麻叶斑病病原菌愈伤组织创伤组织离体筛选植物毒素水稻种质

The association between sexual function and prostate cancer risk in US veterans

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Asian journal of Andrology 2017年第2期19卷 191-195页

作者： Daniel f zapata Lauren E Howard jennifer frank Ross M Simon Cathrine Hoyo Delores j Grant Stephen j freedland Adriana C Vidal Duke University Medical Center Division of Urology Department of Surgery Durham NC 27710 USA Durham Veterans Affairs Medical Center Urology Division Durham NC 27710 USA North Carolina State University Department of Biology Raleigh NC 27695 USA North Carolina Central University Department of Biology Durham NC 27707 USA Department of Surgery Division of Urology Samuel Oschin Comprehensive Cancer institute Cedars-Sinai Medical Center Los Angeles California 90048 USA

Sexual dysfunction and prostate cancer are common among older men. few studies explored the association between these two illnesses. We examined whether sexual function is associated with prostate cancer risk among older men. Among 448 men undergoing prostate biopsy at the Durham Veterans Affairs Hospital, sexual function was ascertained from the Expanded Prostate Cancer Index Composite sexual assessment. We tested the link between sexual function and prostate cancer risk adjusting for multiple demographic and clinical characteristics using logistic regression. Multinomial logistic regression was used to test the associations with risk of low-grade （Gleason 〈6） and high-grade （Gleason 〉7 or 〉4 ＋ 3） disease versus no cancer. Of 448 men, 209 （47%） had a positive biopsy; these men were less likely to be white （43% vs 55%, P=0.013）, had higher prostate-specific antigen （PSA）（6.0 vs 5.4 ng ml-1, P 〈 0.001）, but with lower mean sexual function score （47 vs 54, P = 0.007）. There was no difference in age, BMI, pack years smoked, history of heart disease and/or diabetes. After adjusting for baseline differences, sexual function was linked with a decreased risk of overall prostate cancer risk （OR. 0.91 per lO-point change in sexual function, P= 0.004） and high-grade disease whether defined as Gleason ≥7 （OR： 0.86, P= 0.001） or 〉4 ＋ 3 （OR： 0.85, P= 0.009）. Sexual function was unrelated to low-grade prostate cancer （OR： 0.94, P = 0.13）. Thus, among men undergoing prostate biopsy, higher sexual function was associated with a decreased risk of overall and high-grade prostate cancer. Confirmatory studies are needed.

关键词： erectile dysfunction prostate prostate cancer risk sexual function

Variations of OCT measurements corrected for the magnification effect according to axial length and refractive error in children

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journal of Innovative Optical Health Sciences 2018年第1期11卷 123-137页

作者： Inmaculada Bueno-Gimeno Enrique España-Gregori Andres Gene-Sampedro juan Carlos Ondategui-Parra Carlos j.zapata-Rodriguez Department of Optics and Optometry and Vision Science University of Valencia Spain Department of Surgery University of Valencia University Hospital La FeSpain faculty of Optics and Optometry University Vision Centre Polytechnic University of CataloniaSpain

Purpose:The aim of this paper was to examine the distribution of macular,retinal nerve fiber layer(RNfL)thickness and optic disc parameters of myopic and hyperopic eyes in comparison withemmetropic control eyes and to investigate their variation according to axial length(AL)andspherical equivalent(SE)in healthy ***:This study included 293 pairs of eyes of 293children(145 boys and 148 girks),ranging in age from 6 to 17 years,Subjects were dividedaccording to SE in control(emmetropia,99 children),myopia(100 children)and hyperopia(94children)groups and according to axial AL in 68 short(from≥22.00 mmto 25.00 mm,189)and long eyes(>25.00 mm,36).Macular parameters,RNfL thickness and opticdisc morphology were assessed by the Cirrus^(TM) *** was measured using the ***'s formula was used for calculating the corrected AL-related ocular *** Mean age(±SD)was 10.84±3.05 years;mean(±SD)SE was+0.14±0.51 D(range from8.75 to+8.25 D)and mean AL(±SD)was 23.12±*** RNfL thickness,averagemacular thickness and macular volume decreased as AL and myopia *** correlationsbetween AL/SE and optic disc parameters were found after correcting for magnification ***:AL and refractive error affect measurements of macular and RNfL thickness inhealthy *** make a correct interpretation of ocT measurements,ocular magnificationeffect should be taken into account by clinicians or OCT manufacturers.

关键词： Axial length refractive error children OCT measurements magnification fect

NEURAL NETWORK CLASSIfIER Of jALAPENO CHILE USING IMAGING

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NEURAL NETWORK CLASSIFIER OF JALAPENO CHILE USING IMAGING

Liver steatosis in patients with rheumatoid arthritis treated with methotrexate is associated with body mass index

1996 3rd International Conference on Signal Processing(ICSP’96)

作者： f.Hahn j.L.zapata Dept.Electrical Engineering Instituto Tecnologico de la Laguna Blvd.Revolucion y Czda.Cuauhtemoc 27000 TorreonCoahuilaMEXICO

＜正＞Automatic sorting in the canning industry is searching for simple methods for classifying automatically the *** classifiers can use discriminant analysis but the actual technology available with neural networks is growing everyday due to its advantages of experience-based learning, generalization,graceful degradation and fault *** this paper we present a neural network classifier of jalapeno chile based on chile images obtained with a CCD camera,after being *** image is processed and the noise outside from its perimeter eliminated before calculating features as area,length and *** area,length under different angles are introduced as training data to a propagation algorithm which classifies chiles in three different categories.

关键词： vision neural networks chile jalapeno

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World journal of Hepatology 2023年第5期15卷 699-706页

作者： Agustin Castiella Luis Lopez-Dominguez Maria j Sanchez-Iturri Iratxe Urreta Andrea De Diego joaquin Belzunegui Eva zapata Department of Gastroenterology Service Donostia University HospitalDonostia 20014Spain Department of Rheumatology Donostia University HospitalDonostia 20014Spain Department of Gastroenterology Donostia University HospitalDonostia 20014Spain Department of Clinical Epidemiology Donostia University HospitalDonostia 20014Spain

BACKGROUND Methotrexate(MTX)is the usual first-line treatment for rheumatoid arthritis(RA).Long-term use of MTX has been associated with liver steatosis(LS)and liver fibrosis(Lf).AIM To determine if LS in patients treated with MTX for RA is associated with MTX cumulative dose(MTX-CD),metabolic syndrome(Mt S),body mass index(BMI),the male sex,or *** A single-center,prospective study of patients receiving MTX for RA was performed from february 2019 to february *** inclusion criteria were patients aged 18 years or older diagnosed with RA by a rheumatologist and being treated with MTX(without limitation on the duration of treatment).The exclusion criteria were previous diagnosis of liver disease(hepatitis B or C virus infection,known nonalcoholic fatty liver disease),alcohol consumption greater than 60 g/d in males or 40 g/d in females,human immunodeficiency virus infection on antiretroviral therapy,diabetes mellitus,chronic renal failure,congestive heart failure,or BMI greater than 30 kg/m^(2).Patients receiving leflunomide in the 3 years prior to the study were also *** elastography(fibro Scan,Echosens?,Paris,france)was used for fibrosis determination(Lf>7 Kp A)and computer attenuation parameter(CAP)for LS(CAP>248 d B/m).Demographic variables,laboratory data,MTX-CD(>4000 mg),Mt S criteria,BMI(>25),transient elastography,and CAP scores were collected from all *** fifty-nine patients were ***-three were female(72.88%),and the mean age was 61.52 years(standard deviation:11.73).When we compared MTX-CD≤4000 mg(26 patients;14 with LS and 12 without)with>4000 mg(33 patients;12 with LS and 21 without),no statistical differences were found(P=0.179).We compared CAP scores stratified by Mt S,BMI,sex,and *** were no significant differences in CAP scores based on the presence of Mt S[CAP/Mt S:50 no Mt S(84.75%);9 Mt S(15.25%);P=0.138],the male sex(CAP/sex:8 male/18 female LS;8 male/25 female no LS;P=0.576),or Lf[CAP/fibrosis:

关键词： patients diagnosis index

Prevalence of Isolated “Pre-Malignant” Lesions on Prostate Biopsy in a Racially Diverse Community Screened Cohort

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Open journal of Urology 2015年第12期5卷 213-219页

作者： Michael A. Liss Donna Ankerst David zapata javier Hernandez Robin j. Leach Ian M. Thompson Department of Urology University of Texas Health Science Center San Antonio San Antonio USA Department of Epidemiology and Biostatistics University of Texas Health Science Center San Antonio San Antonio USA Department of Cellular and Structural Biology University of Texas Health Science Center San Antonio San Antonio USA Cancer Therapy and Research Center University of Texas Health Science Center San Antonio San Antonio USA

Objective: We investigated rates of prostate cancer (PCa), high-grade prostatic intraepithelial neoplaisa (HGPIN) and atypical small acinar proliferation (ASAP) in a multiethnic cohort. Methods: We evaluated prostate biopsy outcomes in men enrolled in the San Antonio Center of Biomarkers of Risk for prostate cancer (SABOR) prospective, observational study. PCa-free men underwent annual PSA testing over nearly 14 years with biopsies based on community standards. We investigated biopsy outcomes with a special interest in rates of cancer, HGPIN, and ASAP. Results: We identified 975 prostate biopsies in 801 subjects from 3/1/2001 to 1/9/2014. PCa, HGPIN, or ASAP was encountered in 28.8% (281/975), 10.1% (98/975), and 5.2% (51/975) of prostate biopsy specimens, respectively. The most significant risk factor for a PCa diagnosis was African American race (OR 5.0, 95% CI: 2.2 - 11.4, p fied 57% (24/42) of men diagnosed with a “pre-malignant” lesion on prostate biopsy and had a subsequent biopsy. Of those only 8% (2/24) were diagnosed with prostate cancer (both Gleason 3 + 3) within 1 year of the initial biopsy. Conclusion: We note a 5-fold increased risk of PCa for African American men. The incidence of HGPIN and ASAP are consistent with previously reported incidence. If diagnosed in isolation, repeat biopsy within one year could be delayed or eliminated as it may not change prostate cancer outcomes.

关键词： Active Surveillance Ethnicity Prostate Biopsy Prostate Cancer Race

EARLY VASCULAR GRAfTING TO PREVENT UPPER EXTREMITY NECROSIS AfTER ELECTRICAL BURNS INDICATIONS fOR SURGERY

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中华医学杂志(英文版) 1986年第8期99卷 679-684页

作者：王学威 BrianB.Roberts RomanL.zapata-Sirvent William A.Robenson j.Paul Waymark Edwardj.Law BruceG.Mac Millan Departments of Medicine and Surgery University of Colorado Health Sciences Center Denver Colorado USA Departments of Burns Beijing Jishuitan Hospital and Beijing Institute of Traumatology and Orthopedics Beijing Shriners Burns Institute Cincinnati Ohio USA

In the treatment of electrically burned wrists exhibiting restricted blood circulation, the proper clinical assessment of certain early signs in the period of treatment is of great importance to the reasonable and effective performance of early vascular *** are analysis and summary of six unsuccessful cases and the possible indications for surgery.

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