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The olfactory system of Pieris brassicae caterpllars:from receptors to glomeruli

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Insect Science 2024年第2期31卷 469-488页

作者： Qi Wang Hans M.Smid Marcel Dicke Alexander haverkamp Laboratory of Entomology Wageningen University and ResearchWageningenthe Netherlands

Tolfactory system of adult lepidopterans is among the best described neuronal ***,comparatively little is known about the organization of the olfactory system in the larval stage of these ***,we explore the expression of olfactory receptors and the organization of olfactory sensory neurons in caterpillars of Pieris brassicae,a significant pest species in Europe and a well-studied species for its chemical *** describe the larval olfactory system in this species,we first analyzed the head transcriptome of third-instar larvae(L3)and identified 16 odorant receptors(ORs)including the OR coreceptor(Orco),13 ionotropic receptors(IRs),and 8 gustatory receptors(GRs).We then quantified the expression of these 16 ORs in different life stages,using qPCR,and found that the majority of ORs had significantly higher expression in the L4 stage than in the L3 and L5 stages,indicating that the larval olfactory system is not static throughout caterpillar *** an Orco-specific antibody,we identified all olfactory receptor neurons(ORNs)expressing the Orco protein in L3,L4,and L5 caterpillars and found a total of 34 Orco-positive ORNs,distributed among three sensilla on the *** number of Orco-positive ORNs did not differ among the three larval ***,we used retrograde axon tracing of the antennal nerve and identified a mean of 15 glomeruli in the larval antennal center(LAC),suggesting that the caterpillar olfactory system follows a similar design as the adult olfactory system,although with a lower numerical *** together,our results provide a detailed analysis of the larval olfactory neurons in P brassicae,highlighting both the differences as well as the commonalities with the adult olfactory *** findings contribute to a better understanding of the development of the olfactory system in insects and its life-stage-specific adaptations.

关键词： glomeruli larvae Lepidoptera odorant receptor olfactory receptor neurons sensilla

Mid-term survival of the Optimys short stem: A prospective case series of 500 patients

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World Journal of Orthopedics 2024年第3期15卷 257-265页

作者： Bryan Hamans Sheryl de Waard Sjoerd Kaarsemaker Esther R C Janssen Inger N Sierevelt Gino M M J Kerkhoffs Daniël haverkamp Department of Orthopaedic Surgery Xpert ClinicAmsterdam 1101EANetherlands Department of Orthopedic Surgery Amsterdam University Medical CenterAmsterdam 1105 AZNetherlands Esther R C Janssen Department of Orthopaedic SurgeryViecuri Medisch CentrumVenlo 5912 BLNetherlands Centre for Orthopaedic Research Spaarne ZiekenhuisHoofddorp 2134 TMNetherlands Department of Orthopaedic Surgery and Sports Medicine Amsterdam UMC-Location AMCAmsterdam 1105 AZNetherlands Department of Orthopedic Surgery Xpert Orthopedic Surgery ClinicAmsterdam 1101 EANetherlands

BACKGROUND In recent years,there has been an increase in the number of total hip arthroplasty procedures in the younger patient *** active group has higher expectations of their prosthesis in comparison to the older population,and there is a greater physical demand for the *** femoral stems were in-troduced to retain proximal bone stock and joint biomechanics and became more common to implant in this specific ***,the long-term survival and functional outcomes of various short stems are still being investigated in different *** To determine the 5-year survival of the Optimys hip *** This was a prospective multicenter cohort study of 500 patients conducted in two hospitals in the *** patients received the Optimys short stem(Mathys Ltd,Bettlach,Switzerland).The primary outcome measure was survival of the hip stem,with revision as the *** secondary outcome measurements included patient-reported outcome measures(PROMs).Kaplan-Meier analysis was used to calculate the 5-year survival ***-minus-log transformation was performed to calculate the 95%confidence interval(95%CI).Mixed model analyses were performed to assess the course of the PROMs during the 1st 2 years after *** were modeled separately for the 1st and 2nd years to calculate the yearly change in PROMs during both follow-up periods with accompanying 95%*** The mean age of the total 500 patients was 62.3 years(standard deviation:10.6)and 202 were male(40%).At a median follow-up of 5.5 years(interquartile range:4.5-6.7),7 patients were deceased and 6 revisions were registered,for infection(n=3),subsidence(n=2)and malposition(n=1).This resulted in an overall 5-year survival of 98.8%(95%CI:97.3-99.5).If infection was left out as reason for revision,a stem survival of 99.4%(95%CI:98.1-99.8)was *** questionnaires were completed by 471 patients(94%),317 patients(63%)completed the 1-year follow-up questionna

关键词： Total hip arthroplasty femoral stem Short stem Optimys Survival 5-year survival Revision PROMs

Mid-term clinical outcomes of the uncemented Robert Mathys pressfit cup

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World Journal of Clinical Cases 2024年第19期12卷 3684-3691页

作者： Kishan Ritek Ramsodit Inger N Sierevelt Esther R C Janssen Sjoerd Kaarsemaker Daniël haverkamp Department of Orthopedic Surgery Xpert Clinics AmsterdamAmsterdam 1101EANorth-HollandNetherlands Department of Orthopedic Spaarne Gasthuis AcademyHoofddorp 2134 TMNetherlands Department of Orthopaedic Surgery Viecuri Medical CentreVenlo 5912 BLNetherlands Department of Orthopaedic Surgery Viecuri Medisch CentrumVenlo 5912 BLNetherlands

BACKGROUND The use of uncemented cups during total hip arthroplasty(THA)has gained popularity in recent *** Robert Mathys(RM)pressfit cup,an uncemented monoblock implant is expected to preserve bone density due to its composition and external surface,while reducing backside wear with its monoblock *** factors should lead to a high survival rate of the *** To evaluate the mid-term survival and functional outcome of the RM Pressfit cup in a large study *** Between 2011 and 2020,we included 1324 patients receiving a primary THA using the RM pressfit *** clinical follow-up was performed at 2 years postoperatively with the Dutch arthroplasty register used to assess implant status *** for acetabular failure and reason for revision were reported to evaluate implant survival,while the hip disability and osteoarthritis outcome score(HOOS)scores were used to assess functional *** The mean age at surgery was 64.9 *** mean follow-up was 4.6 *** the 1324 THAs performed,13 needed cup revisions within 5 years after index THA:5 due to aseptic loosening,6 due to infection,2 due to dislocation and 2 due to other *** resulted in a 5-year cup survival of 98.8%(95%CI:98.1-99.5).Nine of the cup revisions occurred within the first year after index *** scores increased significantly in all domains during the first year and levelled out during the second *** In the present study,the RM pressfit cup demonstrated excellent clinical outcomes at mid-term follow-up;however,future studies are needed to assess the long-term outcomes of this acetabular implant.

关键词： Total hip arthroplasty Uncemented RM pressfit cup Hip osteoarthritis Cup revision Pressfit Aseptic loosening

Decision aids can decrease decisional conflict in patients with hip or knee osteoarthritis: Randomized controlled trial

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World Journal of Orthopedics 2021年第12期12卷 1026-1035页

作者： Lode A van Dijk Antonius MJS Vervest Dominique C Baas Rudolf W Poolman Daniel haverkamp Department of Orthopedic Surgery Tergooi HospitalHilversum 1213 XZNoord-HollandNetherlands Department of Orthopedic Surgery Onze Lieve Vrouwe GasthuisAmsterdam 1091 ACNetherlands Department of Orthopedic Surgery Leiden University Medical CentreLeiden 2333 ZANetherlands Department of Orthopedic Surgery Xpert Orthopedie Amsterdam/SCORE(Specialized Center of Orthopedic Research and Education)Amsterdam 1101 EANetherlands

BACKGROUND The interest in shared decision making has increased considerably over the last couple of *** aids(DAs)can help in shared decision *** when there is more than one reasonable option and outcomes between treatments are *** To investigate if the use of DAs decreases decisional conflict in patients when choosing treatment for knee or hip osteoarthritis(OA).METHODS In this multi-center unblinded randomized controlled trial of patients with knee or hip OA were included from four secondary and tertiary referral ***-thirty-one patients who consulted an orthopedic surgeon for the first time with knee or hip OA were included between December 2014 and January *** the first consultation,patients were randomly assigned by a computer to the control group which was treated according to standard care,or to the intervention group which was treated with standard care and provided with a *** the first consultation,patients were asked to complete questionnaires about decisional conflict(DCS),satisfaction,anxiety(PASS-20),gained knowledge,stage of decision making and preferred ***-up was carried out after 26 wk and evaluated decisional conflict,satisfaction,anxiety,health outcomes(HOOS/KOOS),quality of life(EQ5D)and chosen *** After the first consultation,patients in the intervention group(mean DCS:25 out of 100,SD:13)had significantly(P value:0.00)less decisional conflict compared to patients in the control group(mean DCS:39 out of 100,SD 11).The mean satisfaction score for the given information(7.6 out of 10,SD:1.8 vs 8.6 out of 10,SD:1.1)(P value:0.00),mean satisfaction score with the physician(8.3 out of 10,SD:1.7 vs 8.9 out of 10,SD:0.9)(P value:0.01)and the mean knowledge score(3.3 out of 4,SD:0.9 vs 3.7 out of,SD:0.6)(P value:0.01)were all significantly higher in the intervention *** 26-wk follow-up,only 75 of 131 patients(57%)were available for *** sample is too

关键词： Decision aid Decisional conflict Shared decision making Anxiety Hip osteoarthritis Knee osteoarthritis

Kitesurf injury trauma evaluation study:A prospective cohort study evaluating kitesurf injuries

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World Journal of Orthopedics 2020年第4期11卷 243-251页

作者： Christiaan JA van Bergen Rik IK Weber Tim Kraal Gino MMJ Kerkhoffs Daniel haverkamp Department of Orthopedic Surgery Amphia HospitalBreda 4818 CKthe Netherlands Academic Center for Evidence based Sports Medicine Amsterdam 1105 AZthe Netherlands Department of Sports Medicine University Medical CenterUtrecht 3584 CXthe Netherlands Department of Orthopedic Surgery Spaarne HospitalHoofddorp 2134 TMthe Netherlands Department of Orthopedic Surgery Amsterdam University Medical CenterAmsterdam 1105 AZthe Netherlands Department of Orthopedic Surgery Xpert ClinicAmsterdam 1103 TBthe Netherlands

BACKGROUND Kitesurfing is an increasingly popular and potentially dangerous extreme water *** hypothesized that kitesurfing has a higher injury rate than other(contact)sports and that the minority of injuries are *** To investigate the incidence and epidemiology of kitesurfing injuries in a Dutch cohort during a complete kitesurfing *** Injury data of 194 kitesurfers of various skill levels,riding styles and age were surveyed prospectively during a full kitesurf *** participants were recruited through the Dutch national kitesurf association,social media,local websites and kitesurf *** completed digital questionnaires *** amount of time kitesurfing was registered along with all sustained *** an injury was reported,an additional questionnaire explored the type of injury,injury location,severity and the circumstances under which the injury *** The mean age of participants was 31 years(range,13-59)and the majority of the study population was male(74.2%).A total of 177 injuries were sustained during 16816 kitesurf *** calculated injury rate was 10.5 injuries per 1000 h of *** most common injuries were cuts and abrasions(25.4%),followed by contusions(19.8%),joint sprains(17.5%)and muscle sprains(10.2%).The foot and ankle were the most common site of injury(31.8%),followed by the knee(14.1%)and hand and wrist(10.2%).Most injuries were reported to occur during a trick or *** the majority of injuries were mild,severe injuries like an anterior cruciate ligament tear,a lumbar spine fracture,a bimalleolar ankle fracture and an eardrum rupture were *** The injury rate of kitesurfing is in the range of other popular(contact)*** injuries are relatively mild,although kitesurfing has the potential to cause serious injuries.

关键词： Kite boarding Water sports Sports medicine Injuries Epidemiology Extreme sports

Windsurfing vs kitesurfing:Injuries at the North Sea over a 2-year period

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World Journal of Orthopedics 2016年第12期7卷 814-820页

作者： Christiaan JA van Bergen Joris P Commandeur Rik IK Weber Daniel haverkamp Roelf S Breederveld Department of Orthopaedic Surgery Spaarne Gasthuis Department of Surgery Luzerner Kantonsspital Department of Orthopaedic Surgery Slotervaartziekenhuis Department of Trauma Surgery Red Cross Hospital

AIM To analyze all windsurfing and kitesurfing(kiteboarding) injuries presented at our coastal hospital over a 2-year period. METHODS Twenty-five windsurfers(21 male; aged 31 ± 8 years) and 32 kitesurfers(23 male; aged 29 ± 11 years) presented at our hospital during the 2-year study period. Various injury data were recorded,including transport to hospital and treatment. After a median follow-up of 16 mo(range,7-33 mo),18 windsurfers(72%) and 26 kitesurfers(81%) completed questionnaires on the trauma mechanisms,the use of protective gear,time spent on windsurfing or kitesurfing,time to return to sports,additional injuries,and chronic disability. RESULTS Most patients sustained minor injuries but severe injuries also occurred,including vertebral and tibial plateau fractures. The lower extremities were affected the most,followed by the head and cervical spine,the upperextremities,and the trunk. The injury rates were 5.2 per 1000 h of windsurfing and 7.0 per 1000 h of kitesurfing(P = 0.005). The injury severity was the same between groups(P = 1.0). Less than 30% of the study population used protective gear. Kitesurfers had a higher number of injuries,and required transport by ambulance,inpatient hospital stay and operative treatment more often than windsurfers,but these differences were not statistically significant(P > 0.05). The median time to return to windsurfing and kitesurfing was 5 and 4 wk,respectively(P = 0.79). Approximately one-third of the patients in each group experienced chronic *** Kitesurfing results in a significantly higher injury rate than windsurfing in the same environmental conditions but the severity of the injuries does not differ.

关键词： General sports trauma Extreme sports Surfing Epidemiology Prevention

Dutch version of the Victorian Institute of Sports Assessment-Achilles questionnaire for Achilles tendinopathy: Reliability, validity and applicability to non-athletes

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World Journal of Orthopedics 2018年第1期9卷 1-6页

作者： Inger Sierevelt Maayke van Sterkenburg Hans Tol Bella van Dalen Niek van Dijk Daniel haverkamp Department of Orthopaedic Surgery Slotervaart Hospital Department of Orthopedic Surgery Academic Medical Center Academic Center for Evidence Based Sports Medicine Academic Medical Center Amsterdam Bergmanclinics te Naarden

AIM To translate the Victorian Institute of Sports AssessmentAchilles(VISA-A) questionnaire into the Dutch language(VISA-A-NL), and to assess its reliability, validity, and applicability to *** After translation according to a forward-backward protocol, 101 patients with complaints of Achilles tendinopathy were asked to fill out the VISA-A-NL at two time points together with visual analogue scale, the foot and Ankle Outcome Score, and the Short form-36 questionnaires. Reliability, internal consistency, construct validity, and content validity were *** The VISA-A-NL showed high reliability(0.97, 95%CI: 0.95-0.98). Cronbach's alpha(internal consistency) was 0.80. It increased to 0.88 without activity domain. Correlation with other questionnaires was moderate or *** The VISA-A-NL proved to be an excellent evaluation instrument for the Dutch physician. If applied to nonathletes, using a modified score(questions 1-6) should be considered.

关键词： Achilles tendon Victorian Institute of Sports Assessment-Achilles Validity Patient reported outcome measurement

Highly cross-linked versus conventional polyethylene inserts in total hip arthroplasty,a five-year Roentgen stereophotogrammetric analysis randomised controlled trial

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World Journal of Orthopedics 2020年第10期11卷 442-452页

作者： Justin van Loon Daniël Hoornenborg Inger Sierevelt Kim TM Opdam Gino MMJ Kerkhoffs Daniël haverkamp Department of Orthopaedic Surgery Xpert Orthopedie Amsterdam/Specialized Center of Orthopedic Research and EducationAmsterdam 1101EANetherlands Department of Orthopaedic Surgery Amsterdam University Medical CentresLocation Academic Medical CenterAmsterdam 1105AZNetherlands

BACKGROUND Polyethylene(PE)particles produced by wear of the acetabular insert are thought to cause osteolysis and thereby aseptic loosening of the implant in total hip arthroplasty(THA).As highly cross-linked polyethylene(HXLPE)is presumed to give lower wear rates,in vivo studies are needed to confirm *** To compare the wear of REXPOL,a HXPLE,with conventional PE within the first five years after implantation using Roentgen stereophotogrammetric analysis(RSA).METHODS Patients were randomised to receive either a HXLPE(REXPOL)or a conventional PE insert during primary *** images were obtained directly postoperative and after 6 wk,12 wk,6 mo,12 mo,24 mo and five *** outcomes were assessed using the Hip Injury and Osteoarthritis Outcome Score and Harris Hip Score at baseline and five years after *** The HXLPE(REXPOL)showed less wear in the latero-medial *** wear rates of conventional PE were seen in the latero-medial and center-proximal direction and in volume and corrected volume,whereas the REXPOL did not show these outcomes over *** from baseline in functional outcome did not significantly *** Total 3D wear is less in THAs inserted with a REXPOL inlay than a conventional PE inlay after five *** study confirms,for the first,that the REXPOL HXLPE inlay is preferred to standard PE.

关键词： Highly cross-linked polyethylene Primary total hip arthroplasty Polyethylene wear Roentgen stereophotogrammetric analysis

femoroacetabular offset restoration in total hip arthroplasty;Digital templating a short stem vs a conventional stem

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World Journal of Orthopedics 2022年第2期13卷 139-149页

作者： Sheryl de Waard Tom Verboom Niels Hendrik Bech Inger N Sierevelt Gino M Kerkhoffs Daniël haverkamp Orthopedic Surgery Xpert Orthopedie AmsterdamAmsterdam 1101 EANetherlands Orthopaedic Surgery Academic Medical CentreAmsterdam 1105 AZNetherlands

BACKGROUND failure in restoring individual anatomy could be a reason for persistent functional limitations post total hip *** offset(fAO)plays an important role in anatomic restoration,as loss of offset≥5 mm is associated with altered gait and decreased functional *** assessment by use of digital templating has shown to be a reliable method for sizing the components in total hip arthroplasty,and can show if anatomic restoration is *** recent years,short stems are growing in popularity as it could allow better restoration due to more variety in *** To assess whether restoration of the fAO differs between a short or a conventional stem by use of digital ***,association of the preoperative offset and caput-colllum-diaphyseal angle(CCD-angle)within restoration of both stems was investigated,and the reliability of measurements was *** A total of 100 standardized hip radiographs were used for digital *** of fAO was classified into“restored”or“not restored”,when afference from baseline value presented,*** between the two stems concerning proportions of correct restoration of the fAO were analyzed by use of McNemar *** assess association between CCDangle and preoperative fAO with absolute fAO restoration,multi-level analysis was performed by use of a linear mixed model to account for paired *** determination of the optimal point under the curve in operating curve-analysis,bootstrapping of thousand sets was performed to determine the optimal cutoff point of the preoperative fAO for restoration within the limits of 5 *** observers participated for inter-observer reliability,with two observers measuring the radiographs twice for intra-observer *** The mean preoperative fAO was 79.7 mm(range 62.5-113 mm),with a mean CCD-angle of 128.6°(range 114.5°-145°).The conventional stem could only r

关键词： Offset Anatomic offset restoration Total hip arthroplasty Short hip stem Conventional hip stem