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Microbial starch-binding domains are superior to granule-bound starch synthase I for anchoring luciferase to potato starch granules

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Progress in Natural Science:Materials International 2006年第12期16卷 1295-1299页

作者： Jean-Paul VINcKEN Luc c.J.M. SUURS Richard G.f. visser Laboratory of Plant Breeding Department of Plant Sciences Wageningen University Wageningen The Netherlands

Microbial starch-binding domains (SBD) and granule-bound starch synthase I (GBSSI) are proteins which are accumulated in potato starch granules. The eff.c.ency of SBD and GBSSI for targeting active luciferase reporter proteins to granules during starch biosynthesis was compared. GBSSI or SBD sequences were fused to the N- or c-terminus of the luciferase (LUc) gene, via an artif.c.al Pro-Thr encoding linker sequence. The genes were introduced into an amylose-free (amf) potato mutant. It appeared that SBD was superior to GBSSI as a targeting sequence, mainly because the luciferase retained higher activity in the SBD-containing fusion proteins than in the GBSSI-containing ones.

关键词： luciferase GBSS I SBD amf potato.

Same soup different taste-how to best manage the future liver remnant-a surgical perspective

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Hepatobiliary Surgery and Nutrition 2021年第5期10卷 734-736页

作者： John R.Bergquist Patrick Starlinger Brendan c.visser Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryStanford UniversityStanfordCAUSA Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryMayo ClinicRochesterMNUSA

A small but measurable percentage of major hepatic resection patients will experience major morbidity or mortality due post-hepatectomy liver failure after major liver ***-operative optimization,in particular pre-operative induction of liver growth to increase the future liver remnant(fLR)volume,has become frequently used in patients considered to be high ***,this process of hepatic augmentation remains incompletely understood,particularly in *** advances in techniques for fLR management have resulted in dramatic expansion of the liver surgeon’s armamentarium.

关键词： operative hepatic mortality

High-density SNP-based genetic maps for the parents of an outcrossed and a selfed tetraploid garden rose cross, inferred from admixed progeny using the 68k rose SNP array

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Horticulture Research 2016年第1期3卷 68-75页

作者： Mirjana Vukosavljev Paul Arens Roeland E Voorrips Wendy P c van't Westende G D Esselink Peter M Bourke Peter cox W Eric van de Weg Richard G f visser chris Maliepaard Marinus J M Smulders Wageningen UR Plant Breeding Wageningen University&ResearchWageningenNL-6700 AJThe Netherlands Roath BV EindhovenThe Netherlands Pheno Geno Roses D.O.O.Bulevar oslobođenja 65a 21000 Novi SadSerbia

Dense genetic maps create a base for QTL analysis of important traits and future implementation of marker-assisted *** tetraploid rose,the existing linkage maps include<300 markers to cover 28 linkage groups(4 homolog... 详细信息

Dense genetic maps create a base for QTL analysis of important traits and future implementation of marker-assisted *** tetraploid rose,the existing linkage maps includecover 28 linkage groups(4 homologous sets of 7 chromosomes).Here we used the 68k WagRhSNP Axiom single-nucleotide polymorphism(SNP)array for rose,in combination with SNP dosage calling at the tetraploid level,to genotype offspring from the garden rose cultivar‘Red New Dawn’.The offspring proved to be not from a single bi-parental *** rose breeding,crosses with unintended parents occur *** developed a strategy to separate progeny into putative populations,even while one of the parents was unknown,using principle component analysis on pairwise genetic distances based on sets of selected SNP markers that were homozygous,and therefore uninformative for one *** of the inferred populations was consistent with self-fertilization of‘Red New Dawn’.Subsequently,linkage maps were generated for a bi-parental and a self-pollinated population with‘Red New Dawn’as the common maternal *** densest map,for the selfed parent,had 1929 SNP markers on 25 linkage groups,covering 1765.5 cM at an average marker distance of 0.9 *** with the strawberry(fragaria vesca)genome was *** IcM1 corresponded to *** pseudochromosome 7(fv7),IcM4 to fv4,IcM5 to fv3,IcM6 to fv2 and IcM7 to *** IcM2 corresponded to parts of *** pseudochromosomes 1 and 6,whereas IcM3 is syntenic to the remainder of fv6.

关键词： breeding linkage dosage

Duodenal adenocarcinoma: Advances in diagnosis and surgical management

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World Journal of Gastrointestinal Surgery 2016年第3期8卷 212-221页

作者： Jordan M cloyd Elizabeth George Brendan c visser Department of Surgical Oncology University of Texas MD Anderson Cancer Center Department of Surgery Stanford University

Duodenal adenocarcinoma is a rare but aggressive malignancy. Given its rarity, previous studies have traditionally combined duodenal adenocarcinoma(DA) with either other periampullary cancers or small bowel adenocarcinomas, limiting the available data to guide treatment decisions. Nevertheless, management primarily involves complete surgical resection when technically feasible. Surgery may require pancreaticoduodenectomy or segmental duodenal resection; either are acceptable options as long as negative margins are achievable and an adequate lymphadenectomy can be performed. Adjuvant chemotherapy and radiation are important components of multi-modality treatment for patients at high risk of recurrence. further research would benefit from multiinstitutional trials that do not combine DA with other periampullary or small bowel malignancies. The purpose of this article is to perform a comprehensive review of DA with special f.c.s on the surgical management and principles.

关键词： Duodenal cancer Duodenal adenocarcinoma Periampullary Whipple Pancreaticoduodenectomy Segmental resection Small bowel

Blood Volume Status in Patients with chronic fatigue Syndrome: Relation to complaints

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International Journal of.c.inical Medicine 2018年第11期9卷 809-819页

作者： c. (Linda) M. c. van campen frans c. visser Stichting cardioZorg Hoofddorp The Netherlands

four studies have compared a possible decrease in circulating blood volume in Myalgic encephalomyelitis/chronic fatigue syndrome (ME/cfS) patients when compared to a healthy population. A more recent study has proven a correlation between RBc volume and OI in chronic OI patients without being diagnosed ME/cfS. The aim of the present study was to relate measured blood, RBc and plasma volumes (absolute and percent normalized) with the orthostatic intolerance complaints in ME/cfS patients. In the included 11 female ME/cfS patients, percentage decrease in normalized blood, RBc and plasma volume was similar for all three components: 83% ±12%, 83% ±12% and 83% ±11%, respectively. In patients with a clinical suspicion of OI (n = 7) all 3 volume components were signif.c.ntly lower compared to patients without clinical suspicion of OI (n = 4). The difference percentage to normalized Blood volume was 77(7) vs 94(10) (p-value fference percentage to normalized RBc volume was 76(7) vs 96(10) (p-value fference percentage to normalized plasma volume was 77(7) vs 93(10) (p-value c volumes: the relation found was RBc volume = 0.99* Plasma volume + 1.55;p components: RBc and plasma volume may be reduced in ME/cfS patients, especially in the presence of a clinical suspicion of OI.

关键词： Blood Volume chronic fatigue Syndrome Orthostatic Intolerance Red Blood cell Volume

The Eff.c. of.c.rcumin on Patients with chronic fatigue Syndrome/Myalgic Encephalomyelitis: An Open Label Study

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International Journal of.c.inical Medicine 2018年第5期9卷 356-366页

作者： c. (Linda) M. c. van campen K. Riepma frans c. visser Stichting cardiozorg Hoofddorp Netherlands curesupport Valkenburg Netherlands

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/cfS), recently renamed as systemic exertion intolerance disease (SEID), is a chronic and often disabling disease. Although the exact pathophysiological mechanism of ME/cfS is unknown, immunological abnormalities may play an important role. curcumin is a herb with powerful anti-oxidative, and anti-inflammatory properties. Therefore, we hypothesized that curcumin has favorable eff.c.s on symptomatology in ME/cfS patients. In total 52 patients participated, nine stopped the use of.c.rcumin because of side eff.c.s. All remaining patients (n = 43) met the criteria for cfS;72% met the criteria for ME. Before and 8 weeks after the use of.c.rcumin complexed with phosphatidyl choline, 500 mg bid, the cDc inventory for assessment of.c.ronic fatigue Syndrome was filled in. The cDc questions (n = 19) were scored and divided into 2 parts: the first being specif.c.for cfS complaints (n = 9), the second being scores of less specif.c.symptoms (n = 10);denoted as cDc rest score. Results showed that 8 weeks curcumin use signif.c.ntly decreased the cfS related symptom scores, but not the cDc rest scores. Analyzing the data separately for ME and cfS patients, the same signif.c.nce for the cfS symptom scores was present. conclusion: in this open-labeled study, 8 weeks curcumin use in a phosphatidyl choline complex reduced ME/cfS symptomatology. Therefore, a randomized placebo controlled study is warranted to assess its eff.c.cy in ME/cfS patients.

关键词： curcumin chronic fatigue Syndrome Myalgic Encephalomyelitis cDc cfS Symptom Inventory

Quantif.c.tion of the Benef.c.al Eff.c.s of.c.mpression Stockings on Symptoms of Exercise and Orthostatic Intolerance in chronic fatigue Syndrome/Myalgic Encephalomyelitis Patients

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International Journal of.c.inical Medicine 2018年第5期9卷 367-376页

作者： c. (Linda) M. c. van campen freek W. A. Verheugt frans c. visser Stichting cardiozorg Hoofddorp Netherlands Radboud UMc Department of Cardiology Nijmegen Netherlands

chronic fatigue syndrome and myalgic encephalomyelitis (cfS/ME) are, amongst others, characterized by exercise intolerance, pain, post exertional malaise and orthostatic intolerance. It has been shown in venous disease and sport participation that compression stockings may improve exercise performance and reduce post exercise muscle soreness. Moreover, its use is advocated in orthostatic hypotension. Therefore, it was hypothesized that compression stockings may reduce symptomatology in cfS/ME patients. Methods: 100 patients used compression stockings class II for minimally 3 weeks and thereafter filled in a questionnaire, based on the Rand 36 physical activity questions (n = 9), whether compression stockings changed perceived symptoms or not. Moreover, 7 questions referring to prolonged standing and sitting, to recovery post exercise, muscle pain during or immediately post exercise, and to dizziness/light-headedness during or immediately post exercise, while standing and during prolonged sitting were added. Questions were scored as 1: able to perform activity much less while wearing the stockings, 2: perform activity somewhat less, 3: no perceived change in activity, 4: perform activity slightly better, 5: able to perform activity much better while wearing the stockings. Results: In patients able to answer the question, all mean scores per activity were signif.c.ntly higher than 3, being no perceived change in activity while wearing the stockings. Subgroup analysis showed that patients with orthostatic intolerance reported higher eff.c.s than patients without orthostatic intolerance. conclusion: This pilot study suggests that compression stockings may be useful to reduce symptomatology of physical activities in cfS/ME patients, especially in patients with orthostatic intolerance. Larger prospective studies with hard endpoints are warranted.

关键词： chronic fatigue Syndrome Myalgic Encephalomyelitis compression Stockings Exercise Tolerance Orthostatic Intolerance

利用GBSSI和SBD作为“锚”将荧光素酶定位到淀粉粒中效果的比较

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自然科学进展 2006年第6期16卷 679-683页

作者：季勤 Jean-Paul Vincken Krit Raemakers Luc c.J.M.Suurs Richard G.f.visser 淮阴师范学院生物系淮安223300 Laboratory of Plant Breeding Department of Plant SciencesWageningen UniversityWageningenThe Netherlands

利用马铃薯淀粉粒结合型淀粉合成酶Ⅰ(GBSSI)作为“锚”,在马铃薯淀粉粒形成时将荧光素酶(LUc)定位到淀粉粒中．并对GBSSI／LUc重组蛋白质在转基因淀粉粒中的定位与积累、重组蛋白质中GBSSI与淀粉粒的亲和性以及荧光素酶的活性等进行了... 详细信息

利用马铃薯淀粉粒结合型淀粉合成酶Ⅰ(GBSSI)作为“锚”,在马铃薯淀粉粒形成时将荧光素酶(LUc)定位到淀粉粒中．并对GBSSI／LUc重组蛋白质在转基因淀粉粒中的定位与积累、重组蛋白质中GBSSI与淀粉粒的亲和性以及荧光素酶的活性等进行了分析．结果表明,GBSSI／ LUc重组蛋白质在马铃薯淀粉粒中获得了特异性表达,但与以微生物的淀粉粒结合结构域(SBD) 作为“锚”的SBD／LUc重组蛋白质相比较,它与淀粉粒的亲和性以及荧光素酶的活性均下降．由此认为,在淀粉粒形成过程中,利用SBD作为“锚”将荧光素酶蛋白质定位到淀粉粒中的效果要优于GBSSI．

关键词：荧光素酶 GBSSI SBD amf马铃著

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Breeding melon(cucumis melo)with resistance to powdery mildew and downy mildew

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Horticultural Plant Journal 2022年第5期8卷 545-561页

作者： Lei cui Lampros Siskos chen Wang Henk J.Schouten Richard G.fvisser Yuling Bai college of Agriculture Shanxi Agricultural UniversityTaiyuan030031China Plant Breeding Wageningen University&ResearchP.O.Box 3866700 AJWageningenthe Netherlands

Melon(cucumis melo L.)production is often restricted by a plethora of pests and diseases,including powdery mildew and downy mildew caused respectively by the fungal species Podosphaera xanthii/Golovinomyces orontii and oomycete species Pseudoperonospora *** efforts have been directed on identif.c.tion of resistant sources by screening(wild)melon *** the current review,we summarized such efforts from various publications of the last 50 plus *** to powdery mildew has been identified in 239 melon accessions and downy mildew resistance in 452 accessions of both *** and the wild relative species *** the resistance sources,*** *** accessions PMR 45,PMR 5,PMR 6,and WMR 29 as well as *** *** accessions PI 124111,PI 124112,and PI 414723 have been considered as the most valuable germplasm because multiple resistance genes have been identified from these accessions and are widely used in melon resistance *** genetic mapping in a number of resistant sources has enabled identif.c.tion of 25 dominant genes,two recessive genes and seven QTLs conferring powdery mildew resistance,as well as eight dominant genes and 11 QTLs for downy mildew *** on the reported sequences of associated markers,we anchored physically(many of)these genes and QTLs to chromosomes of the melon ***92 *** addition to presenting a comprehensive overview on powdery mildew and downy mildew resistance in(wild)melon germplasm,we suggest strategies aiming at breeding melon with durable and broad-spectrum resistance to pathogens and pests.

关键词： cucumis melo Melon Powdery mildew Downy mildew Resistance Breeding

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