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m.rphological, Cultural, m.lecular and Pathogenic Variation of.Bipolaris sorokiniana in Wheat

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Am.rican Journal of.m.lecular Biology 2022年第4期12卷 190-202页

作者： A. Sharm.n f. m. aminuzzaman T. H. Ansari m. Ahm.d Departm.nt of.Plant Pathology Faculty of Agriculture Sher–e–Bangla Agricultural University Dhaka Bangladesh Plant Pathology Division Bangladesh Rice Research Institute (BRRI) Gazipur Bangladesh

One hundred and twenty isolates of.Bipolaris sorokiniana of.wheat were isolated, identif.ed and characterized based on m.rphological, cultural, pathogenic and ITS (Internal Transcribed Spacer) based m.lecular param.ters. Collected isolates dif.ered in respect of.radial m.celial growth rate, color of.the colony, surf.ce texture of.the colony, shape of.the colony, conidia production ability and shape and color of.conidia. ITS1 and ITS4 based PCR products yielded a species-specif.c band of.600 bp (base pairs) on agarose gel f.r the conf.rm.tion of.B. sorokiniana. The m.celial growth rate of.the isolates ranged f.om.1.39 to 4.46 m./day. The septation of.conidia ranged f.om.1.4 to 8.6. On the basis of.conidia m.rphology, the isolates were grouped into f.ve dif.erent groups, whereas the isolates were grouped into 12 cultural groups based on cultural characteristics. Cultural group 8 [CG (Cultural Group)-8] was the m.st virulent [38.5% LAD (Leaf.area diseased)] and [CG (cultural Group)-4] was the less virulent [2% LAD (Leaf.area diseased)] group am.ng 12 groups when inoculated at tillering stage of.wheat cv. Kachan.

关键词： B. sorokiniana Wheat m.rphological Variation Cultural Variation m.lecular Variation Virulence

Ecof.iendly m.nagem.nt of.Wheat Panicle Blast Caused by m.gnaporthe oryzae triticum

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Agricultural Sciences 2023年第12期14卷 1751-1765页

作者： A. A. m.shuk f. m. aminuzzaman m. R. Islam.K. Nahar A. Sharm.n Departm.nt of.Plant Pathology Faculty of Agriculture Sher-e-Bangla Agricultural University Sher-e-Bangla Nagar Dhaka Bangladesh Departm.nt of.Horticulture Faculty of Agriculture Sher-e-Bangla Agricultural University Sher-e-Bangla Nagar Dhaka Bangladesh

In this study, three wheat varieties were tested to determ.ne seed germ.nation and the incidence of.m.gnaporthe oryzae triticum.(m.T). Am.ng these varieties, BARI Gom.24 (Prodip) wheat seed exhibited the highest seed germ.nation rate (93%) but also had the highest incidence (30%) of.m.T. To m.nage blast disease in an ecof.iendly m.nner, seven treatm.nts were em.loyed: T1 = Control, T2 = Garlic clove extracts, T3 = Aloe vera leaf.extracts, T4 = Black cum.n seed extracts, T5 = Neem.leaf.extracts, T6 = Nativo 75 WG, and T7 = Provax 200 WP. The experim.nt was conducted using a Random.zed Com.lete Block Design (RCBD) layout with three replications using Prodip wheat variety that exhibited highest m.T inf.ction severity based on laboratory analysis am.ng collected varieties. Data were collected on blast disease incidence (%), disease severity, and various growth and yield param.ters of.wheat. The experim.nt’s results indicated that am.ng all the treatm.nts, T7 (Seed treatm.nt with Provax 200 WP) and T5 (f.liar spraying with Neem.leaf.extract) perf.rm.d better in controlling blast disease in wheat. The lowest blast disease incidence (%) was observed with T7 (Provax 200 WP), with values of.7.86, 9.86, and 10.19 recorded during the m.lking stage, sof. dough stage, and hard dough stage of.wheat, respectively. T5 (Neem.leaf.extract) also dem.nstrated a statistically equivalent reduction in blast disease incidence (%). In term. of.disease severity, T7 (Seed treatm.nt with Provax 200 WP) showed the lowest values of.1.03, 1.23, and 1.63 during the m.lking stage, sof. dough stage, and hard dough stage of.wheat, respectively. f.liar spraying with neem.leaf.extract also exhibited sim.lar result as of.Provax 200 WP regarding panicle blast severity. As a result of.these f.ndings, it can be concluded that T5 (Neem.leaf.extract) is recom.ended as

关键词： Wheat Panicle Blast m.gnaporthe oryzae triticum Botanicals m.nagem.nt

Ef.icacy of.Plant Extracts on m.rphology and Cultural Characteristics of.Bipolaris sorokiniana, Causing Black Point Disease of.Wheat in Bangladesh

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Am.rican Journal of.Plant Sciences 2023年第10期14卷 1162-1179页

作者： Dilruba Yesm.n m.. Abul Kalam.Azad f. m. aminuzzaman m.. m.nirul Islam Institute of.Environm.ntal Science University of Rajshahi Rajshahi Bangladesh Departm.nt of.Plant Pathology Faculty of Agriculture Sher-e-Bangla Agricultural University Dhaka Bangladesh

Black point of.wheat caused by Bipolaris sorokiniana (Bs) is very destructive disease of.wheat in Bangladesh and all over the world. The sym.tom. of.diseases caused by Bipolaris sorokiniana can vary depending on the host plant and environm.ntal conditions. The conidia of.Bs germ.nate f.om.two poles, which dif.er physiologically as indicated by the rate, growth character, of.length, breadth and num.er of.septa on dif.erent m.dia. The aim.of.this study was to evaluate the ef.icacy of.selected plant extracts on the m.rphology and cultural characteristics of.the f.ngus Bipolaris sorokiniana causing black point disease in wheat crops in Bangladesh. The ef.icacy of.f.f.een plant extracts on the growth and characteristics of.Bs was conducted in vitro in 2019-2020. The treatm.nts were: Untreated control, T1—Black berry Leaves, T2—Guava Leaves, T3—Lantena cam.ra leaves, T4—Eucalyptus Leaves, T5— Turm.ric Leaves, T6—Khoksha Leaves, T7—Papaya leaves, T8-Gurlic Bulb, T9—Chili dust, T10—Nigella seeds, T11—Turm.ric dust, T12—Cloves, T13— Bohera f.uits, T14—Black pepper and T15—Neem.leaves. Af.er 15 days of.inoculation of.Bipolaris sorokiniana, colony color and shapes were Gerrish blackish, greenish blackish, greyish blackish and shapes were round or irregular. In our study, conidia color was dark brown to light brown, conidiophore color was brown, dark brown, grayish brown, dark olivaceous, light brown and conidia shapes were elliptical, oblong or slightly curved. The septation of.conidia was 2 - 5 and highest septation was seen in T4 and T14 treatm.nts. The highest conidial length was 17.79 μm.recorded in T2 treatm.nt and lowest was 6.62 μm.T9 treatm.nt where conidial breath was 8.27 μm.in control and lowest was 3.79 μm.in T8 treatm.nt. m.celial growth rate of.Bi

关键词： Bipolaris sorokiniana Black Point Botanicals Wheat

In Vitro Ef.icacy Assessm.nt of.Botanical Extracts against Botrytis gladiolorum.Causing Gladiolus Leaf.Blight

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Am.rican Journal of.Plant Sciences 2023年第10期14卷 1146-1161页

作者： m. A. Raham.n m. S. m. Chowdhury m. R. Islam.N. Sultana m. R. Ali N. Akhter f. m. aminuzzaman Departm.nt of.Plant Pathology Faculty of Agriculture Sher-e-Bangla Agricultural University Sher-e-Bangla Nagar Dhaka Bangladesh Departm.nt of.Entom.logy Faculty of Agriculture Sher-e-Bangla Agricultural University Sher-e-Bangla Nagar Dhaka Bangladesh

An experim.nt was conducted at the m.cology laboratory, Departm.nt of.Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh to f.nd out the ef.icacy of.selected botanical extracts against Botrytis gladiolorum.(Bg) causing gladiolus leaf.blight. Inf.cted leaves sam.les were collected f.om.gladiolus f.rm.rs’ f.eld and brought to the laboratory f.r study. Ten selected botanicals were used against the colony growth of.(Bg). The botanicals were m.hendi, Chrysanthem.m. Basil (Tulsi), Onion, Neem. Bael, Arjuna, Garlic, Aloevera (Ghritkum.ry) and Turm.ric. Botanical extracts were applied at the rate of.5%, 10% and 20%. The radial m.celia growth was f.und m.nim.m.(11.60 m.) in garlic extract treated plate at the dose of.5% at 5 DAI, which was statistically sim.lar with turm.ric extract treated (14.00 m.) plate and the inhibition of.growth was 60% and 51.72%, respectively. Sim.lar trend was f.und at 10 DAI and 15 DAI. At 15 DAI, garlic and turm.ric extract gave the best result against Bg, which was statistically sim.lar with onion (50.07% inhibition) and m.hendi (49.93%). All botanicals showed signif.cantly dif.erent results over control and f.und ef.ective in reducing the m.celial growth at the dose of.10%. At 5 DAI, no radial m.celia growth was f.und in garlic treated plate, which was statistically sim.lar with onion treated plate, m.ans that the inhibition of.growth was 100%. Sim.lar trend was also f.und at 10 DAI, and 15 DAI but at 15 DAI, onion (30.20 m.) gave the statistically sim.lar results with Garlic (30.10 m.) and the m.celia growth inhibition was (57.70%) and (57.84%), respectively. In case of.20% dose, garlic extract showed the best result at 5 DAI and the radial m.celia growth was f.und m.nim.m.(00.00 m.) which was statistically sim.lar with onion extract treated (00.00 m.) plate and turm.ric extract treated plate. The inhibition of.growth was 100%. At 15 DAI, the inhibition of.f.ngal growth was f.und (73.74%), (71.23%) and (66.90%), respecti

关键词： Gladiolus Leaf.Blight Botrytis gladiolorum Botanical Extracts Ecof.iendly m.nagem.nt

In VitroEf.icacy of.Botanicals against Rice Blast Pathogen m.gnaporthe oryzae oryzae

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Am.rican Journal of.Plant Sciences 2021年第4期12卷 662-678页

作者： Z. Nazif. f. m. aminuzzaman L. Laila m. K. Rehena Departm.nt of.Plant Pathology Faculty of Agriculture Sher-e-Bangla Agricultural University Dhaka Bangladesh

Rice is the m.st staple cereal crop of.Bangladesh and rice blast caused by m.gnaporthe oryzae oryzae (m.O) has becom. a m.jor f.ctor lim.ting rice yield in Bangladesh and throughout the world. Eight botanicals extract... 详细信息

Rice is the m.st staple cereal crop of.Bangladesh and rice blast caused by Magnaporthe oryzae oryzae (MoO) has become a major factor limiting rice yield in Bangladesh and throughout the world. Eight botanicals extracted both in water and ethanol namely Kalijira (Nigella sativa), Turmeric (Curcuma longa), Ginger (Zingiber officinalis), Garlic (Allium sativum), Onion (Allium cepa), Neem (Azadirachta indica), Allamanda (Allamanda cathartica) and Aloevera (Aloe vera) were tested against MoO in vitro in the Mycology Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University,f.nt-f.m.ly:Verdana;"> Dhaka, Bangladesh. All the botanicals

关键词： Rice Blast m.gnaporthe oryzae oryzae (m.O) Botanical Extracts Ethanol

Phytohorm.ne-Induced Resistance against Xanthom.nas axonopodisPV. citriin Citrus aurantif.lia

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Am.rican Journal of.Plant Sciences 2017年第5期8卷 1135-1147页

作者： Shahran Ahm.d Nayem.m. Salahuddin m. Chowdhury f. m. aminuzzaman Belal Hossain Sahadat Ali Departm.nt of.Plant Pathology Sher-e-Bangla Agricultural University Dhaka Bangladesh Departm.nt of.Horticultural Science Technical University of Munich Freising Germany

Citrus canker is a perilous disease caused by Xanthom.nas axonopodis pv. citri (Xac) which is hardly m.nageable. Till now, chem.cals used against Xac either proved less ef.ective against the disease or hazardous f.r environm.nt. In this study, we investigated how phytohorm.nes such as Salicylic acid (SA, 3 m.), Jasm.nic acid (JA, 0.3 m.), 3-indolacetonitrile (IAN, 0.6 m.), Nicotinic acid (NA, 5 m.), f.lic acid (f., 0.5 m.) inf.uenced canker disease. Disease severity and lesion diam.ter were signif.cantly m.tigated by exogenous application of.these phytohorm.nes com.ared with water (control) accom.anied by the increase of.latent period. However, these phytohorm.nes did not show any in vitro antim.crobial properties against Xac. Concom.tant with boosted resistance, phytohorm.nes treated leaves showed the higher level of.phenyl am.onia lyase (PAL) activity. Besides, total phenolic com.ounds (TPC) and potassium.contents were also higher in phytohorm.ne treated plants com.ared with control. Phytohorm.nes showed the signif.cant and better perf.rm.nce on disease m.tigation than control am.ng which, SA showed the highest perf.rm.nce while JA and INA showed the m.derate perf.rm.nce. However, NA and f. showed the lowest perf.rm.nce. This result recom.ended that application of.som. phytohorm.nes had elicited som. biochem.cal and physiological events which induced the resistance against Xac.

关键词： Citrus Canker Induce Resistance Phenolic Com.ounds Phytohorm.ne

m.rphological, Pathological, Biochem.cal and m.lecular Characterization of.Ralstonia solanacearum.lt;/i>Isolates in Bangladesh

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Am.rican Journal of.m.lecular Biology 2021年第4期11卷 142-164页

作者： S. Razia m. S. m. Chowdhury f. m. aminuzzaman N. Sultana m. Islam Departm.nt of.Plant Pathology Faculty of Agriculture Sher-e-Bangla Agricultural University Dhaka Bangladesh Plant Pathology Division Bangladesh Agricultural Research Institute Joydebpur Gazipur Bangladesh

One thirty sam.les (f.f.y-f.ve potato tubers, twenty-seven potato stem., three chili stem., twenty-eight soil sam.les, f.ve weed sam.les, three banana leaves, and nine water sam.les) were exam.ned and one hundred six (106) Bangladeshi isolates of.m.Ralstonia solanacearum./em. were isolated and identif.ed. Isolation was m.de on selective m.dia (Tetrazolium.chloride m.dia) and m.R. solanacearum./em. was identif.ed based on m.rphological, pathological and biochem.cal properties and polym.rase chain reaction (PCR) by using the species-specif.c prim.rs. Studies showed that 81.54% (106) sam.les were positive on tetrazolium.chloride solid m.dium. Am.ng them.90 isolates were virulent and rest of.them.were avirulent. f.f.y isolates were selected f.r chem.cal characterization based on hypersensitivity test. m.R. solanacearum./em. is gram.negative, aerobic f.cultative bacteria on the basis of.chem.cal characterization. f.f.y tested isolates expressed as race 3 while in biovar test f.rty-eight showed as biovar III and the rest two showed as biovar I. In nine tested isolates f.om.the three districts a species-specif.c band of.280 bp was am.lif.ed in PCR that conf.rm.d the identity of.m.R. solanacerum./em..

关键词： Brown Rot of.Potato Latent Inf.ction Ralstonia solanacearum Biovar Race

m.rphological, Cultural, m.lecular and Pathogenic Variation of.Bipolaris sorokiniana in Wheat

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Ef.ect of.Chem.cal Seed Treatm.nt and BAU-Biof.ngicide on AlternariaBlight (Alternaria brassicae)of.m.stard

Am.rican Journal of.m.lecular Biology 2022年第4期

作者： a. sharm.n f. m. aminuzzaman t. h. ansari m. ahm.d

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Agricultural Sciences 2018年第5期9卷 566-576页

作者： m. Ahm.d N. N. Tonu K. Hornaday f. m. aminuzzaman m. S. m. Chowdhury m. R. Islam Departm.nt of.Plant Pathology Sher-e-Bangla Agricultural University Dhaka Bangladesh University of.British Colum.ia Vancouver Canada

The ef.icacy of.three seed treating chem.cals viz. Provax 200 WP, Brine solution, Rovral 50 WP and one Biof.ngicide viz. BAU-Biof.ngicide were evaluated against Alternaria blight of.m.stard caused by Alternaria brassicae and Alternaria brassicicola. Three varieties viz. var. BARI-6, Tori-7 and SAU-Shorisha-1 were used in this study. The f.eld experim.nt was conducted in the f.rm.of.Sher-e-Bangla Agricultural University, Dhaka and laboratory experim.nt was conducted in the seed health Laboratory, Departm.nt of.Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207 during the period f.om.Novem.er 2008 to July 2009. Am.ng the seed treating chem.cals, Rovral 50WP showed the best perf.rm.nce in reducing disease incidence and severity as well as increasing seed yield. Application of.Rovral 50 WP gave the best result in increasing the num.er of.pod per plant, pod length (cm., num.er of.branch per plant, 100 siliqua weight (g) and 1000 seed weight (g). BAU-Biof.ngicide also showed prom.sing perf.rm.nce in controlling Alternaria blight of.m.stard and reduced 36% and 53% disease incidence and disease severity respectively over untreated control. BAU-Biof.ngicide m.y be recom.ended as an alternative m.ans of.chem.cal f.ngicide such as Rovral 50 WP f.r controlling Alternaria blight of.m.stard.

关键词： m.stard BAU-Biof.ngicide Chem.cal Seed Treatm.nt and Alternaria brassicae