Abstract AIM To investigate whether PMN from patients with IBD or infectious colitis, respectively, secrete increased amounts of pro inflammatory cytokines and can be regulated by IL 10. METHODS Secretion (E...
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Abstract AIM To investigate whether PMN from patients with IBD or infectious colitis, respectively, secrete increased amounts of pro inflammatory cytokines and can be regulated by IL 10. METHODS Secretion (ELISA) as well as corresponding mRNA levels (semiquantitative RT PCR) of pro inflammatory cytokines (IL 1β, TNf α) and of IL 1 receptor antagonist were assessed in peripheral PMN. RESULTS PMN from patients with IBD are primed to secrete enhanced amounts of pro inflammatory cytokines accompanied by detection of corresponding mRNAs in comparison with normal controls. This finding is not specific for IBD but rather reflects intestinal inflammation in general. IL 10 markedly inhibited proinflammatory cytokine secretion as well as corresponding mRNA concentrations. CONCLUSION PMN are an important source of pro inflammatory cytokines in patients with intestinal inflammation and can be downregulated by IL 10.
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