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L-Arginine Supplementation Alters Maternal Blood Biochemical Attributes and Milk Composition Relative to Neonatal Traits of <i>Najdi</i>ewes

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Open Journal of Animal Sciences 2019年第3期9卷 341-354页

作者： Moustafa M. Zeitoun Ahmed A. Al-Ghoneim Khalid A. Al-Sobayil Yasser R. Hassanein Mohamed M. Mansour Kheir El-Din M. El-Azrak Marwa F. A. Attia Tariq i. Almundarij Department of Animal Production and Breeding College of Agriculture and Veterinary Medicine Qassim University Buriedah KSA Department of Animal and Fish Production Faculty of Agriculture Alexandria University El-Shatby Egypt Ministry of Agriculture Riyadh KSA Department of Hematology College of Applied Medical Sciences Qassim University Buriedah KSA Department of Animal Production Faculty of Agriculture Damanhur University Damanhur Egypt Department of Veterinary Medicine College of Agriculture and Veterinary Medicine Qassim University Buriedah KSA

in a 2 × 3 factorial arrangement, 3 levels of L-arginine (0, 75 and 150 mg/kg BW per day) were tested at two stages of pregnancy (Early 60 days vs. Late 60 days) on the maternal blood metabolites and postnatal milk composition relative to the newborn traits of Najdi ewes. Thirty adult pregnant Najdi ewes were randomly assigned into 6 treatments (n = 5). Control early (CE) ewes were orally administered 50 mL normal saline (0.9% NaCl) daily during the first 60 days of pregnancy;control late (CL) ewes were similar to CE except saline was given at the last 60 days of pregnancy;low dose of L-arginine at early pregnancy (LE);low dose of L-arginine at late pregnancy (LL);high dose of L-arginine at early (HE) and high dose of L-arginine at late pregnancy (HL). Blood and milk samples were collected on a weekly basis. Data of lambing and newborn traits were considered. There were non-significant differences in litter size due to L-arginine;however lamb birth and weaning weights increased by 35% in LE-ewes. Arginine did not change blood protein and albumin. Low arginine decreased lipid fractions, creatinine, urea, and milk fat;however, it increased AST, ALT, milk lactose and milk ash. Administration of L-arginine at 75 mg/Kg BW per day for 60 days at an early gestation in ewes improved the maternal body health and milk composition for the welfare of the neonates.

关键词： L-Arginine ewes Fetus Metabolites Lactation Offspring

雌二醇对动情间期切除卵巢母羊黄体生成激素脉冲分泌的抑制作用

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中华内分泌代谢杂志 1990年第3期6卷 163-165页

作者：李江源 i.J.Clarke 中国人民解放军总医院内分泌科澳大利亚亨利王子医院医学研究中心

12只动情间期母羊切除卵巢后,根据随机皮下植入或不植入E_2而分为二组。通过颈静脉插管每5～10分钟采血一次,连续8～9小时,分离血浆作LH放免测定。实验组的平均LH基础分泌水平和LH脉冲幅高比对照组显著减低,LH脉冲频率无明显改变。LH的... 详细信息

12只动情间期母羊切除卵巢后,根据随机皮下植入或不植入E_2而分为二组。通过颈静脉插管每5～10分钟采血一次,连续8～9小时,分离血浆作LH放免测定。实验组的平均LH基础分泌水平和LH脉冲幅高比对照组显著减低,LH脉冲频率无明显改变。LH的平均基础分泌水平和脉冲幅高显著相关。本研究表明,植入E_2抑制垂体LH的分泌,其作用可能主要是抑制LH对GnRH的反应,因此,作用部位是在垂体水平。LH基础分泌水平的下降,亦可能是E_2的抑制作用所致。

关键词：雌二酶黄体生成激素脉冲分泌

Effect of Lipopolysaccharide-induced immune Responses on Pregnancy Loss in ewes

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Open Journal of Animal Sciences 2018年第4期8卷 421-431页

作者： M. R. Graham E. C. Bowdridge S. A. Bowdridge i. Holásková T. H. Elsasser R. A. Dailey Division of Animal and Nutritional Sciences West Virginia University Morgantown USA Office of Statistics Davis College of Agriculture Natural Resources and Design West Virginia University Morgantown USA Animal Bioscience and Biotechnology Laboratory USDA-ARS Beltsville USA

An acute phase response induced by Gram-negative bacteria can reduce pregnancy rate. Early pregnant ewes were used to monitor effects of lipopolysaccharide (LPS), an endotoxin in the outer cell membrane of Gram-negative bacteria, on acute phase/innate immunity response. in Exp. 1, mixed breed ewes were assigned to receive either LPS or LPS and flunixin meglumine, an inhibitor of prostaglandin synthetase, intravenously on day 5 after mating. in Exp. 2, mixed breed ewes were assigned to receive an intravenous injection on day 5 after mating of either saline, LPS, recombinant human tumor necrosis factor (TNF)-α or LPS after pretreatment with dexamethasone. Pregnancy was diagnosed ultrasonographically on d 25, and live births were recorded at parturition. Challenge with LPS induced acute phase responses (fever, mucosal responses, lethargy and increased serum TNF, haptoglobin and?serum amyloid A) and decreased pregnancy rates. Predictably, flunixin meglumine attenuated fever but did not increase pregnancy rate in LPS-treated ewes. Similarly, exogenous TNF alone induced mucosal and serum amyloid A responses but did not affect pregnancy. Pre-treatment with dexamethasone blocked fever and mucosal and lethargic responses and attenuated increases in TNF and haptoglobin but did not ameliorate LPS-induced pregnancy loss. in summary, acute challenge with LPS mimics bacterial-induced pregnancy losses in early pregnant ewes. Although pretreatment with dexamethasone decreased clinical signs and some innate immune responses, neither it nor flunixin meglumine prevented LPS-induced pregnancy loss. That exogenous TNF alone did not promote pregnancy loss indicates that other cytokines also contribute to LPS-induced embryonic loss.

关键词： Acute Phase Response Pregnancy Loss Sheep

牛卵泡液对切除卵巢母羊GnRH、LH和FSH脉冲分泌的调节作用

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中华内分泌代谢杂志 1991年第3期7卷 150-152页

作者：李江源 H.Francis i.J.Clarke 澳大利亚亨利王子医院医学研究中心

给切除卵巢母羊注射活性炭提取的牛卵泡液(n=5)或切除垂体的母羊血清(n=5)后,测定垂体门脉GnRH和外周血LH和FSH。注射牛卵泡液组血浆FSH水平下降50％,而注射去垂体羊血清组无明显改变。两组的GnRH和LH脉冲的幅高和频率相似,表明牛卵泡... 详细信息

给切除卵巢母羊注射活性炭提取的牛卵泡液(n=5)或切除垂体的母羊血清(n=5)后,测定垂体门脉GnRH和外周血LH和FSH。注射牛卵泡液组血浆FSH水平下降50％,而注射去垂体羊血清组无明显改变。两组的GnRH和LH脉冲的幅高和频率相似,表明牛卵泡液抑素选择性的抑制垂体释放FSH,对GnRH分泌无影响。

关键词：卵泡液垂体 GnRH LH FSH

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尿素氨化醉马草的麦角新碱含量及其营养价值

草业科学 1998年第4期15卷 10-13页

作者：邓凯东彭海宏李文蓉 B.E.Warren i.C.Fletcher 新疆畜牧科学院中澳绵羊研究中心澳大利亚西澳农业部中澳绵羊合作研究项目

通过完全随机试验测定了尿素氨化醉马草中毒性物质麦角新碱的含量及氨化醉马草对绵羊的饲喂效果。１２只新疆细毛羊周岁母羊（平均体重２７．３ｋｇ）随机分成２组，每组６只羊，并分别进行如下处理：１）自由采食未处理的醉马草，并每... 详细信息

通过完全随机试验测定了尿素氨化醉马草中毒性物质麦角新碱的含量及氨化醉马草对绵羊的饲喂效果。１２只新疆细毛羊周岁母羊（平均体重２７．３ｋｇ）随机分成２组，每组６只羊，并分别进行如下处理：１）自由采食未处理的醉马草，并每只羊每天给予２５０ｇ精料；２）自由采食经尿素氨化处理的醉马草，并每只羊每天给予２５０ｇ精料。结果表明，用５％尿素溶液对醉马草进行氨化处理（干草：尿素溶液＝１∶１（ｗ／ｖ））显著降低了醉马草中麦角新碱含量达５９．５％（由５６．０降至２２．７ｍｇ／ｋｇ，Ｐ＜０．０５），显著提高了粗蛋白质含量达６０．５％（由８．１％提高到１３．０％，Ｐ＜０．０５），但未显著提高体外干物质消化率（４０．３对４２．２％，Ｐ＞０．０５）。饲养试验表明，绵羊对氨化醉马草的干物质采食量显著高于未处理醉马草（２６７对１６２ｇ／ｄ，Ｐ＜０．０５），但两处理组绵羊的日增重水平差异不显著（－１１６对－１２８ｇ／ｄ，Ｐ＞０．０５）。

关键词：醉马草麦角新碱营养价值增重细毛羊

绵羊供、受体间同、异步胚移对胚胎发育、大小和细胞数量的影响

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上海农业学报 1989年第3期5卷 1-7页

作者：施学仕 M.R.Thomson i.Wilmut 上海市农业科学院畜牧兽医研究所英国AFRC动物生理和遗传研究所爱丁堡研究站

试验从24头同步发情第6天的威尔士供体母羊中挑选出49个优秀桑椹胚,将其移植于5头同步、5头先期发情3天的同种受体母羊子宫内,3天后回收胚胎。结果,同步移植胚胎成活率为66.7％(16/24),异步移植成活率仅20.0％(5/25)。显微照相后的胚胎... 详细信息

试验从24头同步发情第6天的威尔士供体母羊中挑选出49个优秀桑椹胚,将其移植于5头同步、5头先期发情3天的同种受体母羊子宫内,3天后回收胚胎。结果,同步移植胚胎成活率为66.7％(16/24),异步移植成活率仅20.0％(5/25)。显微照相后的胚胎面积,同步组平均每个胚胎为1.51±0.79μm^2(n=11),非同步组为3.47±2.44μm^2(n=4)。胚细胞计数指出,除在异步组发现一特大胚胎的细胞数量约为正常大胚的二倍外,两组间形态大小相似之胚胎的细胞数量无明显差别。

关键词：绵羊同步发情胚胎移植供体受体

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棉籽粕补饲对新疆细毛羊氮代谢的影响

西北农业学报 1999年第1期8卷 23-26页

作者：邓凯东 i.C.Fletcher J.V.Nolan 新疆畜牧科学院中澳绵羊研究中心乌鲁木齐830000 澳大利亚新英格兰大学动物科学系

将24只约15月龄的新疆细毛母羊（平均体重28．9kg）随机分为6个处理组（每处理4只），以基础日粮作对照，混合精料日粮作标准，研究不同水平棉籽粕补饲的效果。结果表明，棉籽粕补饲显著提高了（P＜0．05）绵羊的总氮采食量、氮表观消... 详细信息

将24只约15月龄的新疆细毛母羊（平均体重28．9kg）随机分为6个处理组（每处理4只），以基础日粮作对照，混合精料日粮作标准，研究不同水平棉籽粕补饲的效果。结果表明，棉籽粕补饲显著提高了（P＜0．05）绵羊的总氮采食量、氮表观消化率、氮沉积、尿囊素排出量和由尿囊素排出量估测的瘤胃微生物氮产量。补饲棉籽粕绵羊的瘤胄微生物氮产量显著低于（P＜0．05）饲喂混合精料的绵羊，这可能与前者的能量采食量较低有关。

关键词：干草棉籽粕氮代谢新疆细毛羊细毛羊饲料