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Stachytarpheta cayennensis Aqueous Extract,a New Bioreactor towards Silver Nanoparticles for Biomedical Applications

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Journal of Biomaterials and Nanobiotechnology 2019年第2期10卷 102-119页

作者： Francois Eya’ane Meva Joel Olivier Avom Mbeng Cecile Okalla Ebongue Carsten Schlusener Ulku Kokcam-Demir Agnes Antoinette Ntoumba Phillipe Belle Ebanda Kedi Etienne Elanga evrard-rudy njike loudang Moise Henri Julien Nko’o Edmond Tchoumbi Vandi Deli Christian Chick Nanga Emmanuel Albert Mpondo Mpondo Christoph Janiak Department of Pharmaceutical Sciences Faculty of Medicine and Pharmaceutical SciencesUniversity of DoualaDoualaCameroon Institute for Inorganic Chemistry and Structural Chemistry Heinrich-Heine-University DusseldorfDusseldorfGermany Clinical Biology Laboratory General Hospital of DoualaDoualaCameroon Department of Animal Biology and Physiology Faculty of ScienceUniversity of DoualaDoualaCameroon

This study reports the preparation and characterization of silver nanoparticles synthesized by the mediation of the plant weed Stachytarpheta cayennensis through solution method. Ultraviolet visible spectroscopy (UV-Vis) determines the presence of nanoparticles in the solution. Infrared spectroscopy (IR) proves organic molecules at the particles interface. Powder X-ray diffraction (PXRD) provides phase composition and crystallinity. Shape was showed by scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX) demonstrated the elemental mapping of the silver nanoparticles. Hydrogen peroxide scavenging and phosphomolybdenum antioxidant assays, egg albumin denaturation anti-inflammation study, and the formation mechanism complete the study. The particles have been found composed of pure silver Ag and silver chloride AgCl nanocrystallites. The average crystallite sizes were found to be 13 nm and 20 nm for Ag and AgCl respectively. A Rietveld refinement based XRD pattern data followed by Williamson-Hall plot allows a size and strain analysis. Based on SEM, spherical agglomerates materials were formed and EDX proved the presence of Cl- ions. The reaction formation mechanism of Ag and AgCl is proposed to be simultaneous and competitive. The silver nanoparticles moderately inhibit the denaturation of egg albumin and exhibit antioxidant action;hence, the nanoparticles could be considered as a potential source for biomedical applications.

关键词： Silver Nanoparticles Stachytarpheta cayennensis Size and Strain Anti-Oxidant Anti-Inflammation

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