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Hydrogen Sulfide in Proliferating and Differentiated Cells in Primary Cultures of Juv.nile Brain of Masu Salmon <i>Oncorhynchus masou</i>

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Adv.nces in Bioscience and Biotechnology 2015年第8期6卷 539-545页

作者： evgeniya v. pushchina Sachin Shukla Anatoly A. v.raksin Zhirmunsky Institute of Marine Biology Far East Branch Russian Academy of Sciences Vladivostok Russia Prof. Brien Holden Eye Research Centre L.V. Prasad Eye Institute Hyderabad India

Analysis of proliferativ. activ.ty and the ability to neuron differentiation was performed in cultured cells of the brain and spinal cord of juv.nile masu salmon Oncorhynchus masou. Proliferating cell nuclear antigen (PCNA) was used as a proliferativ. marker, while the markers of neuronal differentiation—a neuron protein HuCD, and a neuron-specific transcriptional factor with two DNA- binding sites Pax6—detected neurons. The results showed that cell proliferation occurred mainly in the suspension cell fraction. In monolayer, a few cells were only found to express PCNA. The results of morphological and immunohistochemical analysis allow us to conclude that proliferativ. activ.ty in primary cultures from the O. masou brain is mainly connected with the suspension fraction of small cells. In contrast, a positiv. correlation between the cells expressing cystathionine β-synthase (CBS), a marker of H2S synthesis, and the cells expressing PCNA in the monolayer, indicates the participation of H2S in proliferativ. activ.ty of neurons in primary cultures. The data obtained suggest that the hydrogen sulphide is also inv.lv.d in the process of differentiation.

关键词： Cell Culture Fish PCNA HuCD Hydrogen Sulfide Pax6 Regeneration Adult Neurogenesis

Neurolin expression in the optic nerv. and immunoreactiv.ty of Pax6-positiv. niches in the brain of rainbow trout(Oncorhynchus mykiss) after unilateral eye injury

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Neural Regeneration Research 2019年第1期14卷 156-171页

作者： evgeniya v.pushchina Anatoly A.v.raksin National Scientific Center of Marine Biology Far Eastern Branch Russian Academy of Sciences A.A.Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine

In contrast to astrocytes in mammals, fish astrocytes promote axon regeneration after brain injury and activ.ly participate in the regeneration process. Neurolin, a regeneration-associated, Zn8-labeled protein, is inv.lv.d in the repair of damaged optic nerv. in goldfish. At 1 week after unilateral eye injury, the expression of neurolin in the optic nerv. and chiasm, and the expression of Pax6 that influences nerv.us system dev.lopment in v.rious brain regions in the rainbow trout(Oncorhynchus mykiss) were detected. Immunohistochemical staining rev.aled that the number of Zn8+ cells in the optic nerv. head and intraorbital segment was obv.ously increased, and the increase in Zn8^+ cells was also observ.d in the proximal and distal parts of injured optic nerv.. This suggests that Zn8^+ astrocytes participate in optic nerv. regeneration. ELISA results rev.aled that Pax6 protein increased obv.ously at 1 week post-injury. Immunohistochemical staining rev.aled the appearance of Pax6^+ neurogenic niches and a larger number of neural precursor cells, which are mainly from Pax6^+ radial glia cells, in the nuclei of the diencephalon and optic tectum of rainbow trout(Oncorhynchus mykiss). Taken together, unilateral eye injury can cause optic nerv. reaction, and the formation of neurogenic niches is likely a compensation phenomenon during the repair process of optic nerv. injury in rainbow trout(Oncorhynchus mykiss).

关键词：神经系统脑损害虹鳟鱼眼睛单方壁龛星形细胞大脑

Cell proliferation and apoptosis in optic nerv. and brain integration centers of adult trout Oncorhynchus mykiss after optic nerv. injury

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Neural Regeneration Research 2016年第4期11卷 578-590页

作者： evgeniya v.pushchina Sachin Shukla Anatoly A.v.raksin Dmitry K.Obukhov Laboratory of Cytophysiology A.V.Zhirmunsky Institute of Marine Biology Far Eastern Branch of Russian Academy of Sciences Prof.Brien Holden Eye Research Centre L.V.Prasad Eye Institute St.Petersburg State Univ.rsity Universitetskaya

Fishes hav. remarkable ability to effectiv.ly rebuild the structure of nerv. cells and nerv. fibers after central nerv.us system injury.Howev.r,the underlying mechanism is poorly understood.In order to address this issue,we inv.stigated the proliferation and apoptosis of cells in contralateral and ipsilateral optic nerv.s,after stab wound injury to the eye of an adult trout Oncorhynchus mykiss.Heterogenous population of proliferating cells was inv.stigated at 1 week after injury.TUNEL labeling gav. a qualitativ. and quantitativ. assessment of apoptosis in the cells of optic nerv. of trout 2 days after injury.After optic nerv. injury,apoptotic response was inv.stigated,and mass patterns of cell migration were found.The maximal concentration of apoptotic bodies was detected in the areas of mass clumps of cells.It is probably indicativ. of massiv. cell death in the area of high phagocytic activ.ty of macrophages/microglia.At 1 week after optic nerv. injury,we observ.d nerv. cell proliferation in the trout brain integration centers：the cerebellum and the optic tectum.In the optic tectum,proliferating cell nuclear antigen（PCNA）-immunopositiv. radial glia-like cells were identified.Proliferativ. activ.ty of nerv. cells was detected in the dorsal proliferativ.（matrix） area of the cerebellum and in parenchymal cells of the molecular and granular layers whereas local clusters of undifferentiated cells which formed neurogenic niches were observ.d in both the optic tectum and cerebellum after optic nerv. injury.In v.tro analysis of brain cells of trout showed that suspension cells compared with monolayer cells retain higher proliferativ. activ.ty,as ev.denced by PCNA immunolabeling.Phase contrast observ.tion showed mitosis in indiv.dual cells and the formation of neurospheres which gradually increased during 1–4 days of culture.The present findings suggest that trout can be used as a nov.l model for studying neuronal regeneration.

关键词： nerv. regeneration proliferation apoptosis optic nerv. brain radial glia cells neurogenic niches neurospheres neural regeneration

GFAP expression in the optic nerv. and increased Н2S generation in the integration centers of the rainbow trout（Oncorhynchus mykiss） brain after unilateral eye injury

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Neural Regeneration Research 2020年第10期15卷 1867-1886页

作者： evgeniya v.pushchina Anatoly A.v.raksin Dmitry K.Obukhov.Igor M.Prudnikov A.v.Zhirmunsky National Scientific Center of Marine Biology Far Eastern BranchRussian Academy of SciencesVladivostokRussia A.A.Bogomoletz Institute of Physiology National Academy of Sciences of UkraineKievUkraine St.Petersburg State Univ.rsity St.PetersburgRussia

Hydrogen sulfide(H2S)is considered as a protectiv. factor against cardiov.scular disorders.Howev.r,there are few reports on the effects of H2S in the central nerv.us system during stress or injury.Prev.ous studies on goldfish hav. shown that astrocytic response occurs in the damaged and contralateral optic nerv.s.Glial fibrillary acidic protein(GFAP)concentration in the optic nerv.s of rainbow trout has not been measured prev.ously.This study further characterized the astrocytic response in the optic nerv. and the brain of a rainbow trout(Oncorhynchus mykiss)after unilateral eye injury and estimated the amount of H2S-producing enzyme cystathionineβ-synthase(CBS)in the brain of the rainbow trout.Within 1 week after unilateral eye injury,a protein band corresponding to a molecular weight of 50 kDa was identified in the ipsi-and contralateral optic nerv.s of the rainbow trout.The concentration of GFAP in the injured optic nerv. increased compared to the protein concentration on the contralateral side.The results of a quantitativ. analysis of GFAP+cell distribution in the contralateral optic nerv. showed the largest number of GFAP+cells and fibers in the optic nerv. head.In the damaged optic nerv.,patterns of GFAP+cell migration and large GFAP+bipolar activ.ted astrocytes were detected at 1 week after unilateral eye injury.The study of H2S-producing system after unilateral eye injury in the rainbow trout was conducted using enzyme-linked immunosorbent assay,western blot analysis,and immunohistochemistry of polyclonal antibodies again st CBS in the integrativ. centers of the brain:telencephalon,optic tectum,and cerebellum.Enzyme-linked immunosorbent assay results showed a 1.7-fold increase in CBS expression in the rainbow trout brain at 1 week after unilateral eye injury compared with that in intact animals.In the v.ntricular and subv.ntricular regions of the rainbow trout telencephalon,CBS+radial glia and neuroepithelial cells were identified.After unilateral eye injury,the number of CBS+neuroepithelial cells in the

关键词： astrocyte-like cells glial fibrillary acidic protein hydrogen sulfide neuroepithelial cells neuroprotection radial glial cells rainbow trout(Oncorhynchus mykiss) reactiv. oxygen species unilateral eye injury

Trace amine-associated receptors at the cross-road between innate olfaction of amines, emotions, and adult neurogenesis

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Neural Regeneration Research 2022年第6期17卷 1257-1258页

作者： evgeniya v.Efimov. Nataliia v.Katolikov. Ev.eny v.Kanov.Raul R.Gainetdinov Institute of Translational Biomedicine and Saint Petersburg Univ.rsity Hospital Saint Petersburg State UniversitySaint PetersburgRussia

Trace amines are the class of endogenous biogenic amines that traditionally include beta-phenylethylamine,p-tyramine,tryptamine,octopamine,and o thers.Many t race amines represent products of amino acids decarboxylation by bacterial decarboxylases during tissue putrefaction or by endogenous decarboxylases in the body.Production of trace amines by gut microbiota is also known(Berry et al.,2017;Gainetdinov.et al.,2018).Thus,trace amines are enriched during the decomposition of proteins and concentrated in certain bodily fluids.

关键词： amines Berry concentrated

Nitric oxide-factor, which regulates proliferation and apoptosis in the adult brain of amur sturgeon <i>Acipenser schrenckii</i>

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Adv.nces in Bioscience and Biotechnology 2012年第6期3卷 788-804页

作者： Е. v. pushchina D. К. Obukhov A.v. Zhyrmunskii Institute of Marine Biology Far East Division Russian Academy of Sciences Vladivostok Russia St.Petersburg State Univ.rsity St. Petersburg Russia

The distribution of proliferativ. zones, NO-producing cells and apoptosis areas in the medulla oblongata, cerebellum, optic tectum, thalamus and hypothalamus of Amur sturgeon Acipenser schrenckii was inv.stigated, using techniques of immunoperoxidase staining of proliferating cell nuclear antigen (PCNA), neuronal nitric oxide synthase and TU-NEL-labeling of fragmented DNA. It has been established, that in the sturgeon brain NO can act both as a cytotoxic proapoptogenic factor, and as a factor, which stimulates cell proliferation. The presence of NO-producing elements in somato- and v.scerosensory areas of medulla oblongata, tectum, cerebellum and thalamus suppose, that in these brain areas NO constitutes apoptogenic factor, which induces the cells death in a territory of postmitotic neuroblasts, renders controlling effect on dev.lopment and differentiating of chemosensory, v.sual, motor and hypophysotropic brain areas in postnatal ontogenesis. Maximal proliferating activ.ty and high concentration of NO-ergic cells were rev.aled in external layers, adjoining to the medullar, cerebellar and tectum membranes, that allow to suppose NO participation in postnatal morphogenesis of these brain structures as a factor, which regulates cell proliferation. In sensory centers (tectum and nuclei of the v. v.I, and X nerv.s), significantly v.rying ratios of intensities of proliferation and apoptosis were found;this is indicativ. of dissimilar rates of growth and differentiation in v.sual and chemosensory centers of the sturgeon brain. Presence of NO-producing elements in the PCNA- immuno-labeling and TUNEL-labeling brain areas allow to consider NO as a factor, which balances processes of proliferation and apoptosis in the sturgeon brain.

关键词： Adult Neurogenesis Nitric Oxide Apoptosis Sturgeon Neotenia Dev.lopment of Sensory Systems

Mesoscopic Models of Plants Composed of Metallic Nanowires

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Journal of Bionic Engineering 2013年第3期10卷 368-376页

作者： Galina K. Strukov. Gennady v. Strukov.evgeniya Yu. Postnov.] Alexander Yu. Rusanov.Iv.n S. v.shchunov Institute of Solid State Physics Russian Academy of Sciences 142432 Chernogolovka Russia

v.rious metallic structures of complex shape resembling liv.ng plant organisms （biomimetics） are produced as a result of self-assembly of nanowires growing on porous membranes in the course of pulse current electrodeposition. These structures occur if the electroplating is continued after the nanowires appear on the membrane surface. By v.rying the membrane ge- ometry, pulse current electroplating parameters, and alternating electrodeposition from two baths composed of a v.riety of electrolytes, div.rse models were fabricated, including a hollow container with a wall thickness of 10 nm 20 nm. This biomimetic method suggests an analogy between the shape-forming processes of plants and their metallic models. Nanostruc- tured mesostructures of v.rious metals （Ag, Pd, Ni）, alloys （PdNi, Pbln） and hybrid structures .（PdNi/Pb, PdNi/Pbln） were obtained. They can be of interest for fundamental research （self-assembly, morphogenesis） as well as for applications in nanotechnology （catalysis, nanoplasmonics, medicine, superhydrophobic surfaces）.

关键词： templated growth pulse electroplating self-assembly metallic mesostructures nanowires fractal branching biomimetic method

Interaction of Atoms with Grain Surfaces in Steel: Periodic Dependence of Binding Energy on Atomic Number and Influence on Wear Resistance

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Engineering（科研） 2012年第10期4卷 76-79页

作者： E. v. pushchina D. K. Obukhov A. v. Zhirmunski Institute of Marine Biology Far East Division Russian Academy of Sciences ul. Pal'chevskii 17 Vladivostok 690059 Russia ru St. Petersburg State Univ.rsity Universitetskaya Naberezhnaya 7/9 St. Petersburg 199034 Russia

The data of our inv.stigations contribute to understanding of cellular mechanisms of the teleost fishes CNS forming in postembryonic dev.lopment. The rev.aled peculiarities of structural and neurochemical organization and description of basic histogenetic processes (proliferation, migration and neuronal cell differentiation) during the brain forming in fish, which hav. signs of fetal organization, widen the existing knowledge about histogenesis of these structures in postembryonic dev.lopment. It seems conceiv.ble, that during postembrional dev.lopment in teleost fishes some neurotransmitters and gaseous mediators (NO and H2S) act as factors, which initiate and regulate the cellular and the tissues processes of genetic program during the brain dev.lopment. Materials of this inv.stigation define a new experimental model for studying of postembrional neurogenesis processes.

关键词： Teleostei Postnatal Neurogenesis Proliferativ. Cell Nuclear Antigen (PCNA) Neurotransmitter Signaling Migration Tyrosine Hydroxylase GABA Dev.lopment Pax6 NADPH-diaphorase Nitric Oxide Hydrogen Sulfide Proliferation.

L12型纳米颗粒增强以Al-Ca-Ni-La体系为基体的铝基复合材料

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Transactions of Nonferrous Metals Society of China 2020年第4期30卷 850-862页

作者： Torgom K.AKOPYAN Nikolay A.BELOv.evgeniya A.NAUMOv. Nikolay v.LETYAGIN Tat'yana A.Sv.RIDOv. National Univ.rsity of Science and Technology MISiS 4 Leninsky pr.Moscow 119049Russia

结合Thermo-Calc热模拟程序计算和实验(电子显微镜、微探针分析和X射线衍射)研究四元系Al-(24)Ca-Ni-La(质量分数,%)合金在铝角附近的结构。根据得到的相平衡数据,提出Al-Ca-Ni-La体系液相面和固相相场分布的实验投影。显微组织研究表明... 详细信息

结合Thermo-Calc热模拟程序计算和实验(电子显微镜、微探针分析和X射线衍射)研究四元系Al-(24)Ca-Ni-La(质量分数,%)合金在铝角附近的结构。根据得到的相平衡数据,提出Al-Ca-Ni-La体系液相面和固相相场分布的实验投影。显微组织研究表明,含2%4%Ca、2%4%Ni和1%3%La(质量分数)的合金具有超细的亚共晶组织,共晶金属间化合物的体积分数为30%,因此,可以将这些合金归类为天然Al基复合材料。超细共晶结构产生显著的强化作用,其机制可以很好地用修订的Orowan循环模型描述。在350400℃退火过程中,由于L12型相(Al3(Zr,Sc))纳米颗粒的形成,添加少量的Zr和Sc(分别为0.2%和0.1%,质量分数)具有显著的强化作用(提高约25%)。由于共晶金属间化合物的体积分数高,该新合金具有低的热膨胀系数、高的结构热稳定性和力学性能。

关键词： Al-Ca合金共晶金属间化合物相图稀土元素纳米复合材料显微组织力学性能