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Chinese Journal of Chemical Engineering 2017年第5期25卷 652-657页

作者： Carlos Eduardo de Araújo Padilha Sérgio Dantas de Oliveira Júnior Domingos Fabiano de Santana Souza Jackson Araújo de Oliveira Gorete Ribeiro de Macedo everaldo silvino dos santos Laboratory of Biochemical Engineering Chemical Engineering DepartmentFederal University of Rio Grande do Norte (UFRN)

A hybrid GMDH neural network model has been developed in order to predict the partition coefficients of invertase from Baker's yeast. ATPS experiments were carried out changing the molar average mass of PEG(1500–6000 Da), p H(4.0–7.0), percentage of PEG(10.0–20.0 w/w), percentage of MgSO_4(8.0–16.0 w/w), percentage of the cell homogenate(10.0–20.0 w/w) and the percentage of MnSO_4(0–5.0 w/w) added as cosolute. The network evaluation was carried out comparing the partition coefficients obtained from the hybrid GMDH neural network with the experimental data using different statistical metrics. The hybrid GMDH neural network model showed better fitting(AARD = 32.752%) as well as good generalization capacity of the partition coefficients of the ATPS than the original GMDH network approach and a BPANN model. Therefore hybrid GMDH neural network model appears as a powerful tool for predicting partition coefficients during downstream processing of biomolecules.

关键词： Partitioning Invertase Aqueous Two Phase System GMDH Neural network

Recovery of polyphenols from camu-camu(Myrciaria dubia ***)depulping residue by cloud point extraction

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Chinese Journal of Chemical Engineering 2018年第12期26卷 2471-2476页

作者： Carlos Eduardo de Araujo Padilha Juliana Chris Silva de Azevedo Francisco Caninde de Sousa Junior Sergio Dantas de Oliveira Junior Domingos Fabiano de Santana Souza Jackson Araujo de Oliveira Gorete Ribeiro de Macedo everaldo silvino dos santos Laboratory of Biochemical Engineering Chemical Engineering Department Federal University of Rio Grande do Notre(UFRN)NatalRNBrazil Laboratory of Food Bioactive Compounds and Dairy Technology Chemical Engineering DepartmentFederal University of Rio Grande do Notre(UFRN)NatalRNBrazil

In this study the potential of cloud point extraction formed by a non-ionic surfactant was used in order to separate polyphenols from industrial residues of camu-camu. The effects of operational conditions of the cloud point extraction(CPE) on the polyphenol recovery and volumetric ratio were investigated. The results showed a maximum recovery of 95.71% that was obtained using 7.0 wt% Triton X-114, native pH(3.25), and 80 wt%polyphenol extract at 30 °C. The use of cloud point extraction was successful to recover the polyphenols from agroindustrial residue since it is a simple as well as of low-cost technique.

关键词： Cloud point extraction Recovery Polyphenols Camu-camu Depulping residue

Production and Recovery of Rhamnolipids Using Sugar Cane Molasses as Carbon Source

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Journal of Chemistry and Chemical Engineering 2010年第11期4卷 27-33页

作者： Ana Carmen santos Marcio Silva Bezerra Heloize dos santos Pereira everaldo silvino dos santos GoreteRibeiro de Macedo Federal University of Rio Grande do Norte Center of Technology Chemical Engineering Department Biochemical Engineering Laboratory Campus Universitario Lagoa Nova 3000 CEP.＇59072-970 Natal Rio Grande do Norte Brazil

Biosurfactants were synthesized by Pseudomonas aeruginosa （P.A.）, using sugar cane molasses as carbon source. Assays were conducted in a shaker with agitation speed of 200 rpm, temperature of 38 ℃ and aeration ratio （Vm/Vf） of 0.4 and 0.6. A concentration of 3.0% was used for the carbon and energy source （molasses） and of 0.3% for the nitrogen source （NaNO3）. Samples were removed at regular times until 96 hours of cultivation. The reduction in surface tension was measured using the ring method; cell concentration was obtained by the dry mass and substrate consumption by the DNS method. The metabolite produced was extracted and quantified by the thioglycolic method. The results showed a maximum surface tension reduction of 46.57% after 60 h, 3.63 g/L of biomass after 8 h （μXmax =0.15 h^-1）, 79.60% of substrate consumption （μs= 0.67 h-1） and 4.47 g/L of rhamnolipid （μp=0.029 h^-1）.

关键词： Biosurfactants molasses rhamnolipids Pseudomonas aeruginosa.

Laminated Solid Timber Slab with Transverse Prestressing Using the Strategy of Interleaved Vertical Displacement of Lamellae

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Journal of Civil Engineering and Architecture 2024年第4期18卷 186-198页

作者： Bianca Bispo dos Reis Jorge Daniel de Melo Moura Marcos Vinício de Camargo everaldo Pletz Graduate Program in Architecture and Urbanism Londrina State UniversityJardim Portal de Versalhes 1Londrina 86057-970PRBrazil Department of Civil Engineering Federal Technological University of Paraná(UTFPR)Apucarana 86812-460PRBrazil Retired professor Department of Structural Engineering Londrina State UniversityLondrina 86057-970PRBrazil

This article presents a study on the structural behavior of transversely prestressed laminated timber slabs,focusing on an innovative approach:vertically misaligned *** misalignment,achieved by sliding vertically the wooden lamellae rather than aligning them,enhances the slab’s cross-section moment of inertia,thereby improving load-bearing capacity and *** involved two groups of structural size specimens:one with vertically aligned lamellae(control group)and the other with misaligned lamellae(study group).Results showed the study group exhibited 42%superior stiffness and 10%less load capacity compared to the *** typically occurred individually in the lamellae,particularly in those with defects or lower modulus of elasticity,concentrated in the middle third of the slabs’free span where tensile stresses *** a higher number of failed lamellae,the study group demonstrated promising *** of prestressing bar indicated no damage at all in the thread,suggesting potential for reducing bar *** findings offer crucial insights into applying these slabs in timber construction as well as to any kind of construction.

关键词： Transversal prestressed slabs wooden construction plantation wood flexural testing design methodology

Role of pancreatography in the endoscopic management of encapsulated pancreatic collections-review and new proposed classification

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World Journal of Gastroenterology 2020年第45期26卷 7104-7117页

作者： Igor Mendonca Proença Marcos Eduardo Lera dos santos Diogo Turiani Hourneaux de Moura Igor Braga Ribeiro Sergio Eiji Matuguma Spencer Cheng Thomas R McCarty Epifanio silvino do Monte Junior Paulo Sakai Eduardo Guimaraes Hourneaux de Moura Gastrointestinal Endoscopy Unit Department of GastroenterologyHospital das ClinicasUniversity of Sao PauloSao Paulo 05403000Brazil Division of Gastroenterology Hepatology and EndoscopyBrigham and Women’s Hospital-Harvard Medical SchoolBostonMA 02115United States

Pancreatic fluids collections are local complications related to acute or chronic pancreatitis and may require intervention when symptomatic and/or *** the last decade,endoscopic management of these collections via endoscopic ultrasound-guided transmural drainage has become the gold standard treatment for encapsulated pancreatic collections with high clinical success and lower morbidity compared to traditional surgery and percutaneous *** understanding of anatomic landmarks,including assessment of the main pancreatic duct and any associated lesions–such as disruptions and strictures-are key to achieving clinical success,reducing the need for reintervention or recurrence,especially in cases with suspected disconnected pancreatic duct ***,proper review of imaging and anatomic landmarks,including collection location,are pivotal to determine type and size of pancreatic stenting as well as approach using long-term transmural indwelling plastic *** to adequately assess the main pancreatic duct may be performed by two methods:Either non-invasively using magnetic resonance cholangiopancreatography or endoscopically via retrograde *** the critical need to understand anatomy via pancrea tography and assess the main pancreatic duct,a standardized approach or uniform assessment strategy has not been described in the ***,the aim of this review was to clarify the role of pancreatography in the endoscopic management of encapsulated pancreatic collections and to propose a new classification system to aid in proper assessment and endoscopic treatment.

关键词： Endoscopic retrograde cholangiopancreatography Endoscopy Endoscopic ultrasound Pseudocyst Endosonography Pancreatic ducts

Pancreatitis after endoscopic retrograde cholangiopancreatography:A narrative review

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World Journal of Gastroenterology 2021年第20期27卷 2495-2506页

作者： Igor Braga Ribeiro Epifanio silvino do Monte Junior Antonio Afonso Miranda Neto Igor Mendonça Proença Diogo Turiani Hourneaux de Moura Mauricio Kazuyoshi Minata Edson Ide Marcos Eduardo Lera dos santos Gustavo de Oliveira Luz Sergio Eiji Matuguma Spencer Cheng Renato Baracat Eduardo Guimarães Hourneaux de Moura Department of Gastrointestinal Endoscopy Unit University of São Paulo School of MedicineSão Paulo 05403-010Brazil

Acute post-endoscopic retrograde cholangiopancreatography pancreatitis(PEP)is a feared and potentially fatal complication that can be as high as up to 30%in high-risk ***-examination measures,during the examination and after the examination are the key to technical and clinical success with a decrease in adverse *** studies have debated on the subject,however,numerous topics remain controversial,such as the effectiveness of prophylactic medications and the amylase dosage *** review was designed to provide an update on the current scientific evidence regarding PEP available in the literature.

关键词： Endoscopic retrograde cholangiopancreatography Pan-creatitis Postendoscopic retrograde cholangiopancreatography pancreatitis Adverse events Pancreatitis Prevention

Climatic Projections of Lightning in Southeastern Brazil Using CMIP5 Models in RCP’s Scenarios 4.5 and 8.5

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American Journal of Climate Change 2017年第3期6卷 539-553页

作者： Ana Paula Paes dos santos Osmar Pinto Júnior Sérgio Rodrigo Quadros dos santos Francisco José Lopes de Lima everaldo Barreiros de Souza André Arruda Rodrigues de Morais Eldo E. ávila Analía Pedernera National Institute for Space Research (INPE) S&atilde o José dos Campos Brazil Vale Institute of Technology (ITV) Belém Brazil National University of Cordoba (UNC) Córdoba Argentina

Given the high and increasing lightning incidence over the Southeast of Brazil and the various impacts that this phenomenon generates to society, there is a growing need in predicting its occurrence, in order to minimize its consequences. In this context, this work presents the development of a methodology for the projection of lightning in the State of S?o Paulo (Southeastern Brazil), using the HadGEM2-ES and CSIRO-Mk3.6 models in two IPCC climate change scenarios: RCP4.5 and RCP8.5. Since lightning is not an output variable of climate models, tests were carried out to evaluate the relationship between the observed data of oceanic and atmospheric fields, which are known as outputs of the models, and the lightning from the RINDAT and BrasilDAT detection networks. As result, a correlation of 0.84 was obtained. In the projections, it was verified that, while during a large portion of the current climate we observed events of lightning below the average, the future climate reveals the preponderance of anomalously above average events, both in the scenario of intermediate-low emissions (RCP4.5) and in the scenario of high emissions (RCP8.5), suggesting a change in the pattern of the lightning incidence in the State of S?o Paulo.

关键词： Lightning Climatic Projections Southeastern Brazil