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Multi‑omics integration identifies regulatory factors underlying bovine subclinical mastitis

Journal of Animal Science and Biotechnology 2024年第3期15卷 987-1007页

作者： Mengqi Wang Naisu Yang Mario Laterriere David Gagne Faith Omonijo eveline M.Ibeagha‑Awemu Agriculture and Agri‑Food Canada Sherbrooke Research and Development CentreSherbrookeQCCanada Department of Animal Science Universite LavalQuebec CityQCCanada Quebec Research and Development Centre Agriculture and Agri-Food CanadaQuebec CityQCCanada

Background Mastitis caused by multiple factors remains one of the most common and costly disease of the dairy ***-omics approaches enable the comprehensive investigation of the complex interactions between mul-tiple layers of information to provide a more holistic view of disease ***,this study investigated the genomic and epigenomic signatures and the possible regulatory mechanisms underlying subclinical mastitis by integrating RNA sequencing data(mRNA and lncRNA),small RNA sequencing data(miRNA)and DNA methylation sequencing data of milk somatic cells from 10 healthy cows and 20 cows with naturally occurring subclinical mastitis caused by Staphylococcus aureus or Staphylococcus *** Functional investigation of the data sets through gene set analysis uncovered 3458 biological process GO terms and 170 KEGG pathways with altered activities during subclinical mastitis,provided further insights into subclin-ical mastitis and revealed the involvement of multi-omics signatures in the altered immune responses and impaired mammary gland productivity during subclinical *** abundant genomic and epigenomic signatures with sig-nificant alterations related to subclinical mastitis were observed,including 30,846,2552,1276 and 57 differential methylation haplotype blocks(dMHBs),differentially expressed genes(DEGs),lncRNAs(DELs)and miRNAs(DEMs),***,5 factors presenting the principal variation of differential multi-omics signatures were *** important roles of Factor 1(DEG,DEM and DEL)and Factor 2(dMHB and DEM),in the regulation of immune defense and impaired mammary gland functions during subclinical mastitis were *** of the omics within Factors 1 and 2 explained about 20%of the source of variation in subclinical ***,networks of impor-tant functional gene sets with the involvement of multi-omics signatures were demonstrated,which contributed to a comprehensive view of the possible regu

关键词： Candidate discriminant multi-omics signature Gene Long non-coding RNA Methylation haplotype block MicroRNA Multi-omics integration Natural killer cell mediated cytotoxicity pathway Staphylococcus aureus infection pathway

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Therapeutic Route of Patients with Cirrhosis in Bangui

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Open Journal of Gastroenterology 2020年第4期10卷 88-96页

作者： Serges Magloire Camengo Police Peggy Gué rendo Georges Service Benoî t Elowa Gaé tan Adouaka eveline mofini Armelo Thibaut Yangba Kalebanga Nathalie Philomè ne Boua-Akelelo Bernard Bessanguem Joseph Rogevr Molowa Kobendo Department of Hepato-Gastroenterology and Internal Medicine of “Amitié Sino-Centrafraine” University Hospital Center Bangui Central African Republic Department Venerology Dermatology National University Hospital Center Bangui Central African Republic Department of Internal Medicine “Maman Elisabeth Domitien” University Hospital Center Bimbo Central African Republic

Introduction: Cirrhosis is the final stage in the development of any chronic liver disease. The objective of our study was to determine the therapeutic route of patients with cirrhosis. Patients and Methods: This was a one-year cross-sectional study of consenting patients of both sexes with cirrhosis who were hospitalized for the first time. The study took place from October 1, 2013 to September 30, 2014. The diagnosis of cirrhosis was based on clinical, biological and morphological arguments. Results: In total, 1028 patients were hospitalized, including 202 for cirrhosis (19.6%). One hundred and four patients (10.1%) met our inclusion criteria. These were 70 men (67.3%) and 34 women (32.7%). The sex ratio was 2.05. The average age was 43.74 ± 13.74 years. In 68 cases (65.4%), cirrhosis was considered a supernatural disease and in 36 cases (34.6%) was a natural disease. At the first signs, 88 patients (84.6%) had consulted (traditional healer in 34 cases, pastor in 25 cases, marabout in 16 cases and general practitioner in 13 cases). The pastor prayed to 25 patients (24%). The traditional healer made the patients ingest herbal preparations (n = 18), associated or not with scarification (n = 4) and/or enema (n = 28). The marabout recited Quranic verses written on a board, then washed and ingested in 16 cases (15.4%). The general practitioner prescribed treatment to the 13 patients (12.5%). The various treatments received had led to hepatic encephalopathy (21 cases), digestive hemorrhage (15 cases), abdominal pain (3 cases), jaundice (3 cases), increased abdominal bloating (21 cases). The patients had consulted the specialist within an average of 74 ± 15.3 days. The average time to hospital was 21 days ± 13. Conclusion: Cirrhosis is diagnosed at the complication stage due to inappropriate treatment by pastors, marabouts and the general practitioner. The population must be made aware of the early use of health care facilities.

关键词： Therapeutic Route Cirrhosis Bangui

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The Epidemiological, Clinical, Biological and Morphological Characteristics of Primitive Liver Cancers in Bangui

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Open Journal of Gastroenterology 2020年第4期10卷 97-105页

作者： Serges Magloire Camengo Police Georges Service Nathalie Philomè ne Boua-Akelelo Diane N’guilé Benoî t Elowa Timothé e Mobima Francky Kouandogui Bangué eveline mofini Yangba Kalebanga Armelo Thibaut Bessanguem Bernard Boniface Koffi Department of Hepatogastroenterology and Internal Medicine of “Amitié Sino-Centrafraine” University Hospital Center Bangui Central African Republic Department of Internal Medicine “Maman Elisabeth Domitien” University Hospital Center Bimbo Central African Republic Department of Radioloy and Medical Imaging of “Amitié Sino-Centrafraine” University Hospital Center Bangui Central African Republic Department of Radioloy and Medical Imaging of “Maman Elisabeth Domitien” University Hospital Center Bimbo Central African Republic Department Pathological Anatomy National Laboratory of Clinical Body and Public Health Bangui Central African Republic

Objective: To describe the epidemiological, clinical, biological and morphological aspects of primary liver cancer (PLC). Patients and Methods: We conducted a 38-month cross-sectional study in the Department of hepatogas-troenterology and Internal Medicine “Amitié Sino-Centrafraine” University Hospital Center in Bangui. Included in the study were all patients with a diagnosis of PLC. The PLC’s diagnostic arguments were the large tumor liver associated or not with the elevation of alpha-fetoprotein, the heteronodular liver hypervascularized on abdominal ultrasound. Data analysis was done using Epi Info 3.5.1 software. Results: We collected 115 cases of CPF among 2410 hospitalized patients (4.7%). There were 86 men and 29 women (sex ratio: 2.9). The average age was 50 years old. The main risk factors were alcohol consumption (72.2%) and chronic hepatitis B infection (67.4%). Frequent clinical signs were pain in the right hypochondrium and/or epigastric (93.86%), large tumor liver under examination (91.3%), weight loss (74.78%). The serum alpha-fetoprotein concentration was ≥ 400 ng/ml in 73% of the cases. The abdominal ultrasound found a heteronodular liver in all patients. The nodules were multiple hyperechoic in 66.1% of the cases. According to the Child-Pugh classification, the patients were classified as B (49.5%) and C (33.9%). The Okuda Classification ranked patients at stage 1 in 16.5% cases, stage 2 in 52.1% cases and stage 3 in 31.3% cases. According to the BCLC classification, 5.2% of patients were in stage A, 12.2% in stage B, 52.2 in stage C and 30.4% in stage D. Death was recorded during hospitalization in 89 cases (77.4%). Conclusion: CPF is a frequent and serious pathology in Bangui. Its diagnosis is often late, preventing curative treatment. The main causes are alcohol consumption and the hepatitis B virus. The population should be educated to reduce the incidence of this disease.

关键词： Primary Liver Cancer Epidemiology Clinical Morphology Bangui

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High Prevalence of Hepatitis B Virus Infection Compared to Human Immunodeficiency Virus among Blood Donors in Bangui

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Open Journal of Gastroenterology 2020年第6期10卷 137-143页

作者： Serges Magloire Camengo Police Bernard Bessanguem eveline mofini Benoît Elowa Georges Service Peggy Guéréndo Armelo Thibaut Yangba Kalebanga Nathalie Philomène Boua-Akélélo Tolmbaye Sem Fiacre Odilon Joseph Roger Molowa Kobendo Department of Hepatogastroenterology and Internal Medicine of “Amitié Sino-Centrafraine” University Hospital Center Bangui Central African Republic Department of Internal Medicine “Maman Elisabeth Domitien” University Hospital Center Bimbo Central African Republic Department of Venerology Dermatology National University Hospital Center Bangui Central African Republic

Introduction: Hepatitis B Virus (HBV) and Human Immunodeficiency Virus (HIV) infection is a public health problem worldwide, particularly in sub-Saharan Africa. Objective: to compare the epidemiological, clinical and biological characteristics of chronic HBV and HIV infection in blood donors at the National Center for Blood Transfusion (NCBT) in Bangui. Patients and Methods: This was an 8-month analytical cross-sectional study from August 10, 2011 to April 9, 2012. During this study, we consecutively enrolled consenting blood donors of both sexes in which the search for HBsAg and HIV infection was carried out. Results: During the study period, 850 blood donors were collected. HBsAg was found in 142 donors (16.7%), of whom 55 blood donors (6.5%) were coinfected with HIV. On the other hand, HIV serology was positive in 77 blood donors (9.1%) including 55 co-infected (6.5%) with HBV. In order to better compare the risk factors, we have not included HIV-HBV coinfected patients. Only 795 blood donors were selected for the risk factor study. There were 87 cases of HBsAg positive (10.9%) and 22 cases of HIV positive (2.8%). The average age of HIV and HBV infected patients was 25.7 and 26.2 years

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Prevalence of HBsAg and Antibodies to Hepatitis C Virus among Female Sex Workers in Bangui

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Open Journal of Gastroenterology 2020年第6期10卷 144-150页

作者： Serges Magloire Camengo Police Nathalie Philomène Boua-Akélélo Georges Service eveline mofini Benoît Elowa Armelo Thibaut Yangba Kalebanga Bernard Bessanguem Marcel Mbeko Simaléko Peggy Guérendo Henri Diemer Jospeh Roger Molowa Kobendo Department of Hepato-Gastroenterology and Internal Medicine of “Amitié Sino-Centrafraine” University Hospital Center Bangui Central African Republic Department of Internal Medicine “Maman Elisabeth Domitien” University Hospital Center Bimbo Central African Republic National Reference Center for Sexually Transmitted Infections and Antiretroviral Therapy Bangui Central African Republic Department of Public Health Faculty of Health Sciences University of Bangui Bangui Central African Republic Department Venerology Dermatology National University Hospital Center Bangui Central African Republic

Introduction: Hepatitis B virus (HBV) and Hepatitis C Virus (HCV) infection is a public health problem. Sex workers remain a vulnerable group. Objective: To determine the prevalence of HBsAg and hepatitis C virus (HCV) antibody transport among female sex workers (FSW) in Bangui. Patients and Methods: All FSW carriers of HBsAg and/or anti-HCV Antibodies were collected in a 6-month cross-sectional analytical study. The HBsAg research used the Monolisa HBsAg® (BioMérieux®) technique, sandwich ELISA. HCV serology consisted of the search for anti HCV antibodies by monolisa HCV (BioMérieux®), Elisa of the sandwich type. HIV serology used two Elisa tests (Genscreen® and Vironostika®). Data analysis was done using Epi Info 3.5.1 software. The Chi2 test was used for comparison with a signif

关键词： HBsAg Anti HCV Antibodies Female Sex Workers Bangui

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Normal Range of Neutrophile to Lymphocyte Ratio in Healthy, Chronic Kidney Disease Stage 4 - 5 and Hemodialysis Participant in a Sub-Saharan African Context

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Open Journal of Nephrology 2024年第1期14卷 104-114页

作者： Fouda Menye Epse Ebana Hermine Danielle Elimby Lionel Thierry Sevele Deussi De Ngaha Bogne Takam Yvan Halle Marie-Patrice eveline Ngouadjeu Dongho Department of Medicine and Specialties General Hospital of Douala Douala Cameroon Department of Internal Medicine and specialties Faculty of Medicine and Biomedical Sciences of Yaounde Yaounde Cameroon Department of Internal Medicine and specialties Faculty of Medicine and Pharmaceutic Sciences of Douala Douala Cameroon

Background: Neutrophile to lymphocyte ratio (NLR) is a well-established marker of inflammation. It has been reported to be high in chronic kidney disease (CKD) and it is described as a prognosis factor in hemodialysis patients. However, limited data are available about the normal range of NLR in healthy adults as well as CKD patients including hemodialysis black Africans in Sub-Saharan countries. We sought to study NLR in healthy and advanced CKD in a single Cameroonian health facility. Methods: Blood samples were obtained from blood donors, CKD stage 4 and non-dialysis stage 5, and hemodialysis patients for more than 6 months. Patients with confounding factors such as positive CRP, infection, and recent use of steroids were excluded. RNL means standard deviations (SDs), and 95% confidence intervals (95% CI) were determined. RNL range was defined as percentiles P2.5 to P97.5. Results: A total of 102 participants were included. Mean age was 40.45 ± 9.97 years and 58.8% were male. Age and sex distribution were similar in the three groups. Leucopenia and neutropenia were common in all the groups. Means NLR was 1.29 ± 0.57 (95% CI 1.08 - 1.49) range between 0.55 to 2.67;2.31 ± 1.3 (95% CI 1.75 - 2.88) with range between 0.69 to 4.31 and 2.14 ± 0.98 (95% CI 1.85 - 2.44) with range between 0.77 to 4.32 respectively in controls, CKD, and hemodialysis participants. NLR was comparable in CKD and hemodialysis patients (p = 0.99). Compared to controls, NLR was significantly elevated in CKD (p = 0.043) and hemodialysis patients (p Conclusion: Our data suggest that the normal range for NLR in adult nongeriatric healthy and advanced CKD patients including those on chronic hemodialysis may vary between 0.55 to 2.67 and 0.69 to 4.32 respectively.

关键词： NLR CKD Hemodialysis Cameroon

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Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

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Journal of Animal Science and Biotechnology 2023年第2期14卷 593-613页

作者： Mengqi Wang Nathalie Bissonnette Mario Laterriere Pier‑Luc Dudemaine David Gagne Jean‑Philippe Roy Xin Zhao Marc‑Andre Sirard eveline M.Ibeagha‑Awemu Sherbrooke Research and Development Centre Agriculture and Agri-Food CanadaSherbrookeQuebecCanada Department of Animal Science Laval UniversityQuebecQuebecCanada Quebec Research and Development Centre Agriculture and Agri-Food CanadaQuebecQuebecCanada Department of Clinical Sciences Universite de MontrealSt‑HyacintheQuebecCanada Department of Animal Science McGill UniversitySte‑Anne‑De‑BellevueQuebecCanada.

Background:Mastitis caused by different pathogens including Streptococcus uberis(***)is responsible for huge economic losses to the dairy *** order to investigate the potential genetic and epigenetic regulatory mecha‑nisms of subclinical mastitis due to ***,the DNA methylome(whole genome DNA methylation sequencing)and transcriptome(RNA sequencing)of milk somatic cells from cows with naturally occurring *** subclinical mastitis and healthy control cows(n=3/group)were ***:Globally,the DNA methylation levels of CpG sites were low in the promoters and first exons but high in inner exons and *** DNA methylation levels at the promoter,first exon and first intron regions were nega‑tively correlated with the expression level of genes at a whole‑genome‑wide *** general,DNA methylation level was lower in ***‑positive group(SUG)than in the control group(CTG).A total of 174,342 differentially methylated cytosines(DMCs)(FDR<0.05)were identified between SUG and CTG,including 132,237,7412 and 34,693 DMCs in the context of CpG,CHG and CHH(H=A or T or C),***,101,612 methylation haplotype blocks(MHBs)were identified,including 451 MHBs that were significantly different(dMHB)between the two groups.A total of 2130 differentially expressed(DE)genes(1378 with up‑regulated and 752 with down‑regulated expression)were found in *** of methylome and transcriptome data with MethGET program revealed 1623 genes with signifi‑cant changes in their methylation levels and/or gene expression changes(MetGDE genes,MethGET P‑value<0.001).Functional enrichment of genes harboring≥15 DMCs,DE genes and MetGDE genes suggest significant involvement of DNA methylation changes in the regulation of the host immune response to *** infection,especially cytokine ***,discriminant correlation analysis with DIABLO method identified 26 candidate biomarkers,including 6 DE genes,15 CpG‑DMCs and 5 dMHBs that discrimina

关键词： Discriminant biomarkers Gene expression Genome‑wide DNA methylation pattern Immune processes and pathways Methylation haplotype block Milk somatic cell Streptococcus uberis Subclinical mastitis

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Newton-Multigrid for Biological Reaction-Diffusion Problems with Random Coefficients

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Numerical Mathematics(Theory,Methods and Applications) 2012年第1期5卷 62-84页

作者： eveline Rosseel Nico Scheerlinck Stefan Vandewalle Computer Science Department Katholieke Universiteit LeuvenCelestijnenlaan 200AB-3001 LeuvenBelgium

An algebraic Newton-multigrid method is proposed in order to efficiently solve systems of nonlinear reaction-diffusion problems with stochastic *** problems model the conversion of starch into sugars in growing *** stochastic system is first converted into a large coupled system of deterministic equations by applying a stochastic Galerkin finite element *** method leads to high-order accurate stochastic solutions.A stable and high-order time discretization is obtained by applying a fully implicit Runge-Kutta *** Newton linearization,a point-based algebraic multigrid solution method is *** order to decrease the computational cost,alternative multigrid preconditioners are *** results demonstrate the convergence properties,robustness and efficiency of the proposed multigrid methods.

关键词： Multigrid stochastic Galerkin finite element method reaction-diffusion problems implicit Runge-Kutta method and PDEs with random coefficients

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Heme oxygenase-1 as a therapeutic target in inflammatory disorders of the gastrointestinal tract

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World Journal of Gastroenterology 2010年第25期16卷 3112-3119页

作者： Vijith Vijayan Sebastian Mueller eveline Baumgart-Vogt Stephan Immenschuh Institute for Anatomyand Cell Biology Justus-Liebig-University Giessen 35392 Gies-sen Germany Salem Medical Center and Center of Al-cohol Research University of Heidelberg 69121 HeidelbergGermany Institute for Transfusion Medicine Hannover Medical School 30625 Hannover Germany

Heme oxygenase (HO)-1 is the inducible isoform of the first and rate-limiting enzyme of heme degradation. HO-1 not only protects against oxidative stress and apoptosis, but has received a great deal of attention in recent years because ofits potent anti-inflammatory functions. Studies with HO-1 knockout animal models have led to major advances in the understanding of how HO-1 might regulate inflammatory immune responses, although little is known on the underlying mechanisms. Due to its beneficial effects the targeted induction of this enzyme is considered to have major therapeutic po- tential for the treatment ofinflammatory disorders. This review discusses current knowledge on the mechanisms that mediate anti-inflammatory protection by HO-1. More specifically, the article deals with the role of HO-1 in the pathophysiology of viral hepatitis, inflammatorybowel disease, and pancreatitis. The effects of specific HO-1 modulation as a potential therapeutic strategy in experimental cell culture and animal models of these gastrointestinal disorders are summarized. In conclusion, targeted regulation of HO-1 holds major promise for future clinical interventions in inflammatory diseases of the gastrointestinal tract.

关键词： Antioxidant Heme oxygenase Hepatitis Immunity Inflammation Inflammatory bowel disease Oxidative stress Pancreatitis

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Nonlinear Stochastic Galerkin and Collocation Methods:Application to a Ferromagnetic Cylinder Rotating at High Speed

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Communications in Computational Physics 2010年第10期8卷 947-975页

作者： eveline Rosseel Herbert De Gersem Stefan Vandewalle Computer Science Department K.U.LeuvenCelestijnenlaan 200A3001 LeuvenBelgium Faculty of Sciences K.U.LeuvenEtienne Sabbelaan 538500 KortrijkBelgium

The stochastic Galerkin and stochastic collocation method are two state-ofthe-art methods for solving partial differential equations(PDE)containing random *** the latter method,which is based on sampling,can straightforwardly be applied to nonlinear stochastic PDEs,this is nontrivial for the stochastic Galerkin method and approximations are *** this paper,both methods are used for constructing high-order solutions of a nonlinear stochastic PDE representing the magnetic vector potential in a ferromagnetic rotating *** model can be used for designing solid-rotor induction machines in various machining tools.A precise design requires to take ferromagnetic saturation effects into account and uncertainty on the nonlinear magnetic material *** issues of the stochastic Galerkin method are addressed and a numerical comparison of the computational cost and accuracy of both methods is *** stochastic Galerkin method requires in general less stochastic unknowns than the stochastic collocation approach to reach a certain level of accuracy,however at a higher computational cost.

关键词： Nonlinear PDE with random coefficients polynomial chaos stochastic collocation method stochastic Galerkin method electromagnetics

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