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Dominance of Brassica and No Effects of Raphanus in Mature Seed Production in Intergeneric Hybrid between Brassica rapa ssp. Pekinensis and Raphanus

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American Journal of Plant Sciences 2022年第3期13卷 416-432页

作者： Soo-Seong Lee Cho Yee Son eunsil kim Hosub Shin Jeong Eun Park Seung Hwa Yu Jin Hoe Huh BioBreeding Institute Anseong Gyeonggi South Korea Department of Agriculture Forestry and Bioresources College of Agriculture & Life Science Seoul National University Seoul South Korea

We succeeded in producing mature seed from a line of Brassica rapa ssp. pekinensis that had been hybridized with Raphanus sativus var. major. Our focus was on dominance of B. rapa ssp. pekinensis;radish (R. sativus var. major) had no influence. Marker tests for similarity showed that the original CR291M-64 x HwiM-2 hybrid was an inbred CR291M-64, rather than a genuine cross;this appears to have resulted from weak self-incompatibility in this strain. The plants from the mature seed bloomed with reddish flowers differently shown up to present. The intergeneric hybrid between Brassica inbred and Raphanus hybrid was very weak in strength compared to the Brassica inbred which was self-pollinated even though the cause of the weak was not identified. The hybrids between Brassica hybrid, dominant and elite recessive, and Raphanus can be developed in large quantities using mature hybrid seed without resorting to ovule culture techniques.

关键词： Intergeneric Hybrid Brassica Dominance No Raphanus Effect Mature Seed

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Diagnosis of gastric epithelial neoplasia:Dilemma for Korean pathologists

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World Journal of Gastroenterology 2011年第21期17卷 2602-2610页

作者： Joon Mee kim Mee-Yon Cho Jin Hee Sohn Dae Young Kang Cheol Keun Park Woo Ho kim So-Young Jin Kyoung Mee kim Hee Kyung Chang eunsil Yu Eun Sun Jung Mee Soo Chang Jong Eun Joo Mee Joo Youn Wha kim Do Youn Park Yun Kyung Kang Sun Hoo Park Hye Seung Han Young Bae kim Ho Sung Park Yang Seok Chae Kye Won Kwon Hee Jin Chang The Gastrointestinal Pathology Study Group of Korean Society of Pathologists Department of Pathology Inha University Hospital Department of Pathology Yonsei University Wonju College of Medicine Wonju Christian Hospital Department of Pathology Sungkyunkwan University School of MedicineKangbuk Samsung Medical Center Department of Pathology Chungnam National University College of Medicine Department of Pathology Sungkyunkwan University School of MedicineSamsung Medical Center Department of Pathology Seoul National University College of Medicine Department of Pathology Soonchunhyang University Hospital Department of Pathology Kosin University College of Medicine Department of Pathology University of Ulsan College of MedicineAsan Medical Center Department of Pathology The Catholic University of KoreaSeoul St.Mary's Hospital Department of Pathology Seoul National University Boramae Hospital Department of Pathology Eulji General Hospital Department of Pathology Inje University Ilsan Paik Hospital Department of Pathology Kyunghee University College of Medicine Department of Pathology Pusan National University College of Medicine Department of Pathology Inje University Seoul Paik Hospital Department of Pathology Korea Cancer Center Hospital Department of Pathology Konkuk University Medical Center Department of Pathology Ajou University School of Medicine Department of Pathology Chonbuk National University Medical School Department of Pathology Korea University College of Medicine Department of Pathology Bundang Jesaeng Hospital Department of Pathology National Cancer Center The Korean Society of Pathologists 4FThe Korean Medical Association BuildingSeoul 140-721South Korea

The histopathological diagnosis of gastric mucosal biopsy and endoscopic mucosal resection/endoscopic submucosal dissection specimens is important,but the diagnostic criteria,terminology,and grading system are not the same in the East and West.A structurally invasive focus is necessary to diagnose carcinoma for most Western pathologists,but Japanese pathologists make a diagnosis of cancer based on severe dysplastic cytologic atypia irrespective of the presence of *** the Vienna classification was introduced to reduce diagnostic discrepancies,it has been difficult to adopt due to different concepts for gastric epithelial neoplastic *** pathologists experience much difficulty making a diagnosis because we are influenced by Japanese pathologists as well as Western *** is geographically close to Korea,and academic exchanges are ***,Korean doctors are familiar with Western style medical *** a result,the terminology,definitions,and diagnostic criteria for gastric intraepithelial neoplasia are very heterogeneous in *** solve this problem,the Gastrointestinal Pathology Study Group of the Korean Society of Pathologists has made an effort and has suggested guidelines for differential diagnosis:(1) a diagnosis of carcinoma is based on invasion;(2) the most important characteristic of low grade dysplasia is the architectural pattern such as regular distribution of crypts without severe branching,budding,or marked glandular crowding;(3) if nuclear pseudostratification occupies more than the basal half of the cryptal cells in three or more adjacent crypts,the lesion is considered high grade dysplasia;(4) if severe cytologic atypia is present,careful inspection for invasive foci is necessary,because the risk for invasion is very high;and(5) other structural or nuclear atypia should be evaluated to make a final decision such as cribriform pattern,papillae,ridges,vesicular nuclei,high nuclear/cytoplasmic ratio,loss

关键词：病理诊断粘膜上皮理学家胃粘膜韩国学术交流活动发育不良细胞学

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Genomic change in hepatitis B virus associated with development of hepatocellular carcinoma

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World Journal of Gastroenterology 2016年第23期22卷 5393-5399页

作者： Danbi Lee Heather Lyu Young-Hwa Chung Jeong A kim Priya Mathews Elizabeth Jaffee Lei Zheng eunsil Yu Young Joo Lee Soo Hyung Ryu Department of Internal Medicine University of Ulsan College of MedicineAsan Medical Center Sidney kimmel Comprehensive Cancer Center Johns Hopkins University School of MedicineBaltimore Department of Pathology University of Ulsan College of MedicineAsan Medical Center Department of Hepatobiliary Surgery University of Ulsan College of MedicineAsan Medical Center Department of Internal Medicine Inje Uni-versity College of MedicineSeoul Paik Hospital

AIM: To determine the genomic changes in hepatitis B virus(HBV) and evaluate their role in the development of hepatocellular carcinoma(HCC) in patients chronically infected with genotype C ***: Two hundred and forty chronic hepatitis B(CHB) patients were subjected and followed for a median of 105 mo. HCC was diagnosed in accordance with AASLD guidelines. The whole X, S, basal core promoter(BCP), and precore regions of HBV were sequenced using the direct sequencing ***: All of the subjects were infected with genotype C HBV. Out of 240 CHB patients, 25(10%) had C1653 T and 33(14%) had T1753 V mutation in X region; 157(65%) had A1762T/G1764 A mutations in BCP region, 50(21%) had G1896 A mutation in precore region and 67(28%) had pre-S deletions. HCC occurred in 6 patients(3%). The prevalence of T1753 V mutation was significantly higher in patients who developed HCC than in those without HCC. The cumulative occurrence rates of HCC were 5% and 19% at 10 and 15 years, respectively, in patients with T1753 V mutant, which were significantly higher than 1% and 1% in those with wild type HBV(P < 0.001).CONCLUSION: The presence of T1753 V mutation in HBV X-gene significantly increases the risk of HCC development in patients chronically infected with genotype C HBV.

关键词： Hepatocellular carcinoma Chronic hepatitis B Genomic change Hepatitis B virus Genotype C

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