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Insect Science 2008年第5期15卷 415-422页

作者： Dong Hun Kim Sang-chul lee Do-Yeon Kwak Kyeong-Yeoll lee School of Applied Biosciences College of Agricultura and Life Sciences Kyungpook National University Daegu Yeongnam Agricultural Research Institute Milyang Republic of Korea

Three heat shock protein （HSP） genes （hsp70, hsc70, hsp90） were partially cloned from the brown planthopper Nilaparvata lugens and the small brown planthopper Laodelphax striatellus （Homoptera： Delphacidae）, which are serious pests of the rice plant. Sequence comparisons at the deduced amino acid level showed that the three HSPs of planthoppers were most homologous to corresponding HSPs of dipteran and lepidopteran species. Identities of both heat shock cognate 70 and HSP90 were higher than HSP70 in both species. Identity of the HSP70 between the two planthopper species was only 81%, a value much lower than seen among fly and moth groups. Effects of heat and cold shocks were demonstrated on expression of the three hsp genes in the two planthopper species. Heat shock （40 ℃） upregulated the hsp90 level but did not change the hsc70 level in either the nymph and adult stages of either species. On the other hand, the hsp70 level was only upregulated in L. striatellus. This heat shock response was prompt and lasted only for 1 h after treatment. In contrast, cold shock at 4℃ did not change the expression levels of any hsp in either species.

关键词： cold shock gene expression heat shock heat shock protein genes planthoppers thermal stress

Ex situ echo sounder target strengths of ice krill Euphausia crystallorophias

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Chinese Journal of Oceanology and Limnology 2015年第3期33卷 802-808页

作者： Hyoung Sul LA Hyungbeen lee Donhyug KANG SangHoon lee Hyoung chul SHIN Division of Polar Ocean Environment Research Korea Polar Research Institute Korea Institute of Ocean Science and Technology Maritime Security Research Center Korea Institute of Ocean Science and Technology

Ice krill is the keystone species in the neritic ecosystem in the Southern Ocean, where it replaces the more oceanic Antarctic krill. It is essential to understand the variation of target strength （TS in dB re l m^2） with the different body size to accurately estimate ice krill stocks. However, there is comparatively little knowledge of the acoustic backscatter of ice krill. The TS of individual, formalin-preserved, tethered ice krill was measured in a freshwater test tank at 38, 120, and 200 kHz with a calibrated split-beam echo sounder system. Mean TS was obtained from 21 individual ice krill with a broad range of body lengths （L： 13-36 iron）. The length （L, mm） to wet weight （W; mg） relationship for ice krill was 11/=0.001 21g^103~L35s （R2=0.96）. The mean TS-to-length relationship were TS38kHz=-177.4＋57log10（L）, （R^2=0.86）; TS120kHz= -129.9＋31.561ogf0（L）, （R2=0.87）; and TS200kHz=-117.6＋24.661ogre（L）, （R2=0.84）. Empirical estimates of the relationship between the TS and body length of ice krill were established at 38, 120, and 200 kHz and compared with predictions obtained from both the linear regression model of Greene et al. （1991） and the Stochastic Distorted Wave Born Approximation （SDWBA） model. This result might be applied to improve acoustic detection and density estimation of ice krill in the Southern Ocean. Further comparative studies are needed with in situ target strength including various body lengths of ice krill.

关键词： ice krill Euphausia crystallorophias ex situ target strength split-beam echo sounder

A study on the interference effects for tidal current power rotors

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Science China(Technological Sciences) 2010年第11期53卷 3094-3101页

作者： chul-Hee JO Kang-Hee lee Jin-Young YIM Department of Naval Architecture & Ocean Engineering Inha University

Due to global warming, the need to secure an alternative resource has become more important nationally. With the high tidal range of up to 9.7 m on the west coast of Korea, numerous tidal current projects are being planned and constructed. To extract a significant quantity of power, a tidal current farm with a multi-arrangement is necessary in the ocean. The rotor, which initially converts the energy, is a very important component because it affects the efficiency of the entire system, and its performance is determined by various design variables. The power generation is strongly dependent on the size of the rotor and the incoming flow velocity. However, the interactions between devices also contribute significantly to the total power capacity. Therefore, rotor performance considering the interaction problems needs to be investigated for generating maximum power in a specific field. This paper documents a performance study of devices considering the interference between rotating rotors with axial, transverse and diagonal arrangements.

关键词： TCP (tidal current power) renewable energy interference effect rotor CWC (circulation water channel) CFD (computational fluid dynamics)

Beach stabilization by the laying of a drainage layer

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Science China(Technological Sciences) 2012年第9期55卷 2625-2639页

作者： HUR Dong-Soo lee Woo-Dong CHO Won-chul Department of Ocean Civil Engineering(Institute of Marine Industry) Gyeongsang National University445 Inpyeong-dongTongyeong 650-160Korea Department of Ocean Civil Engineering Gyeongsang National University445 Inpyeong-dongTongyeong 650-160Korea Department of Civil and Environmental Engineering Chung-Ang University221 Heuksuk-dong Dongjak-guSeoul 156-756Korea

In the present study, the stabilization effects of the laying of a drainage layer in a sandy beach were analyzed. The numerical model developed by Hur et al. （2011） was used to examine the characteristics of wave-sandy beach interaction with/without a drainage layer. This numericai model directly simulates Wave-Structure-Seabed/Sandy （WASS） beach interaction by consid- ering flow through a porous medium with inertia, laminar and turbulent resistances, and determines the eddy viscosity with the Large Eddy Simulation （LES） turbulent model in a 3-D wave field （LES-WASS-3D）. Based on the numerical results, it ap- pears that the installation of a drainage layer in the beach results in a decrease in both the mean groundwater level around the foreshore and the flow moving offshore on the beach surface. Spatial distributions of the mean groundwater level and wave setup around the foreshore with various arrangements of the drainage layer and conditions of the incident wave are also discussed in this paper.

关键词： drainage layer groundwater level foreshore LES-WASS-3D (3-D numerical simulation) beach stabilization swash zone

Asymmetric optical camouflage:tuneable reflective colour accompanied by the optical Janus effect

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Light(Science & Applications) 2020年第1期9卷 325-334页

作者： Taehyun Kim eui-Sang Yu Young-Gyu Bae Jongsu lee In Soo Kim Seok Chung Seung-Yeol lee Yong-Sang Ryu Sensor System Research Center Korea Institute of Science and TechnologySeoul 02792Republic of Korea Department of Micro/Nano Systems Korea UniversitySeoul 02841Republic of Korea School of Electronic and Electrical Engineering Kyungpook National UniversityDaegu 41566Republic of Korea Nanophotonics Research Center Korea Institute of Science and TechnologySeoul 02792Republic of Korea School of Mechanical Engineering Korea UniversitySeoul 02841Republic of Korea

Going beyond an improved colour gamut,an asymmetric colour contrast,which depends on the viewing direction,and its ability to readily deliver information could create opportunities for a wide range of applications,such as nextgeneration optical switches,colour displays,and security features in anti-counterfeiting ***,we propose a simple Fabry–Perot etalon architecture capable of generating viewing-direction-sensitive colour contrasts and encrypting pre-inscribed information upon immersion in particular solvents(optical camouflage).Based on the experimental verification of the theoretical modelling,we have discovered a completely new and exotic optical phenomenon involving a tuneable colour switch for viewing-direction-dependent information delivery,which we define as asymmetric optical camouflage.

关键词： asymmetric generating readily

Germline-competent stem cell in avian species and its application

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Asian Journal of Andrology 2015年第3期17卷 421-426页

作者： Jae Yong Han Hyung chul lee Tae Sub Park Department of Agricultural Biotechnology College of Agriculture and Life Sciences and Research Institute of Agriculture and Life Sciences Seoul National University Seoul 151-921 Korea Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology Seoul National University Pyeongchang-gun Gangwon-do 232-916 Korea

Germ cells are the only cell type in the body that can transfer genetic information to the next generation. Germline-competent stem cells can self-renew and contribute to the germ cell lineage giving rise to pluripotent stem cells under specific conditions. Hence far, studies on germline-competent stem cells have contributed to,the generation of avian model systems and the conservation of avian genetic resources. In this review, we focus on previous studies on germline-competent stem cells from avian species, mainly chicken germline-competent stem cells, which have been well established and characterized. We discuss different sources of germline-competent stem cells and recent advances for the future applications in birds.

关键词： birds germline-competent stem cells primordial germ cells spermatogonial stem cells

Influence of the wastewater composition on denitrification and biological P-removal in the S-DN-P process: (c) Dissolved and undissolved substrates

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Journal of Environmental Sciences 2009年第8期21卷 1074-1079页

作者： Hee-Jeong Choi chul-Ho Choi Seung-Mok lee Diwakar Tiwari Department of Environmental Engineering Kwandong University Department of Chemistry School of Physical Sciences Mizoram University

The denitdfication and P-removal in the sorption-denitrification-P-removal （S-DN-P） process were carded out under various wastewater compositions. It is noted that P-removal largely depends on the wastewater composition as well to the quantity of the substrates present in wastewater fraction. Three different wastewater fractions are obtained as： raw wastewater, dissolved wastewater （obtained with filtration using 0.45 μm filter）, and undissolved wastewater （i.e., infiltrate obtained by above filtration）. The ratio of P-release/CODtotal-consumption clearly inferred that undissolved wastewater possess very low value i.e., 0.0008 followed by raw wastewater 0.008 and dissolved wastewater 0.03. When this ratio was nearby 0.01, enhanced P-removal was observed. Moreover, the ratio of P-uptake to NO3^--N decomposition for raw wastewater was two times for dissolved wastewater. Interestingly, it was observed that the P-removal and denitrification depend not only on the dissolved substrates but also the undissolved substrates present in the wastewater. The result of the P-removal obtained with this S-DN-P process did not show a big difference of 36%, 34% and 30%, respectively, for raw, dissolved and undissolved wastewater.

关键词： denitrification polyhydroxybutyrate sequencing batch biofilm reactor sorption denitrification P-removals

Genomic organization and transcriptional modulation in response to endocrine disrupting chemicals of three vitellogenin genes in the self-fertilizing fish Kryptolebias marmoratus

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Journal of Environmental Sciences 2016年第4期28卷 187-195页

作者： Bo-Mi Kim Min chul lee Hye-Min Kang Jae-Sung Rhee Jae-Seong lee Department of Biological Science College of ScienceSungkyunkwan University Department of Marine Science College of Natural SciencesIncheon National University

Vitellogenin（Vtg）is the precursor of egg yolk proteins,and its expression has been used as a reliable biomarker for estrogenic contamination in the aquatic *** examine the biomarker potential of the self-fertilizing killifish Kryptolebias marmoratus Vtgs（Km-Vtgs）,full genomic DNAs of Km-Vtgs-Aa,Km-Vtgs-Ab,and Km-Vtgs-C were cloned,sequenced,and *** Vtg genes in *** are tandemly placed in a550 kb section of the same *** silico analysis of promoter regions revealed that both the Km-Vtgs-Aa and Km-Vtgs-Ab genes had an estrogen response element（ERE）,but the Km-Vtgs-C gene did ***,all three Km-Vtgs genes had several ERE-half sites in their promoter *** analysis demonstrated that the three deduced amino acid residues were highly conserved with conventional Vtgs protein,forming distinctive clades within teleost *** tissue showed the highest expression of Km-Vtg transcripts in all tested tissues（brain/pituitary,eye,gonad,intestine,skin,and muscle）in response to endocrine disrupting chemical（EDC）-exposed ***-Vtg transcripts were significantly increased in response to 17β-estradiol（E2）,tamoxifen（TMX）,4-n-nonylphenol（NP）,bisphenol A（BPA）,and octylphenol（OP）over 24 hr *** Km-Vtg-A gene was highly expressed compared to the control in response to NP and ***-induced modulatory patterns of Km-Vtg gene expression were different depending on tissue,gender,and isoforms.

关键词： Kryptolebias marmoratus Vitellogenin Endocrine disrupting chemical

Relaxation of rabbit cavernous smooth muscle to 17β-estradiol: a non-genomic, NO-independent mechanism

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Asian Journal of Andrology 2004年第2期6卷 127-131页

作者： Sae-chul Kim Kyung-Kun Seo Soon-chul Myung Moo Yeol lee Department of Urology College of Medicine Chung-Ang University Seoul 140-757 KoreaDepartment of Urology College of Medicine Chung-Ang University Seoul 140-757 KoreaDepartment of Urology College of Medicine Chung-Ang University Seoul 140-757 KoreaDepartment of Physiology College of Medicine Chung-Ang University Seoul 140-757 Korea

Aim: To investigate whether estrogen was involved in relaxation of rabbit cavernous smooth muscle. Methods: Relaxation response of the rabbit cavernous smooth muscles to 17β-estradiol (0.3, 3, 30 and 300 nmol/L) were observed in vitro. The response of the muscle strips to estrogen after incubation with either actinomycin D (10μmol/L) or L-NAME (10μmol/L) were also evaluated. Inside-out mode of patch clamp in a single smooth muscle cell was applied to investigate the Maxi-K channel activities. Results: Estrogen caused a dose-dependent relaxation of the strips precontracted with norepinephrine. The maximal response was noted about 10 minutes after treatment. The estrogen-induced relaxation was prevented by neither actinomycin D nor L-NAME, suggesting that the response was not mediated by gene transcription or nitric oxide (NO). Application of 17β-estradiol increased the Maxi-K channel activities. Conclusion: 17β-estradiol may be involved in relaxation of rabbit cavernous smooth muscles via a non genomic and NO independent mechanism. 17β-estradiol stimulates Maxi-K channel of the rabbit cavernous myocyte.

关键词： 17β-estradiol cavernous smooth muscles Maxi-K channel relaxation

High-throughput computational-experimental screening protocol for the discovery of bimetallic catalysts

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npj Computational Materials 2021年第1期7卷 1232-1241页

作者： Byung chul Yeo Hyunji Nam Hyobin Nam Min-Cheol Kim Hong Woo lee Sung-chul Kim Sung Ok Won Donghun Kim Kwan-Young lee Seung Yong lee Sang Soo Han Computational Science Research Center Korea Institute of Science and TechnologySeoulRepublic of Korea Department of Energy Resources Engineering Pukyong National UniversityBusanRepublic of Korea Materials Architecturing Research Center Korea Institute of Science and TechnologySeoulRepublic of Korea Department of Chemical and Biological Engineering Korea UniversitySeoulRepublic of Korea Department of Nanomaterials Science and Engineering Korea University of Science and TechnologyDaejeonRepublic of Korea Advanced Analysis Center Korea Institute of Science and TechnologySeoulRepublic of Korea

To accelerate the discovery of materials through computations and experiments,a well-established protocol closely bridging these methods is *** introduce a high-throughput screening protocol for the discovery of bimetallic catalysts that replace palladium(Pd),where the similarities in the electronic density of states patterns were employed as a screening *** first-principles calculations,we screened 4350 bimetallic alloy structures and proposed eight candidates expected to have catalytic performance comparable to that of *** experiments demonstrate that four bimetallic catalysts indeed exhibit catalytic properties comparable to those of ***,we discover a bimetallic(Ni-Pt)catalyst that has not yet been reported for H_(2)O_(2) direct *** particular,Ni_(61)Pt_(39) outperforms the prototypical Pd catalyst for the chemical reaction and exhibits a 9.5-fold enhancement in cost-normalized *** protocol provides an opportunity for the catalyst discovery for the replacement or reduction in the use of the platinum-group metals.

关键词： synthesis catalyst bimetallic