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The LEAFY floral regulator displays pioneer transcription factor properties

Molecular Plant 2021年第5期14卷 829-837页

作者： Xuelei Lai Romain Blanc-Mathieu Loïc GrandVuillemin Ying Huang Arnaud Stigliani Jeremy Lucas Emmanuel Thevenon Jeanne Loue-Manifel Laura Turchi Hussein Daher eugenia brun-hernandez Gilles Vachon David Latrasse Moussa Benhamed Renaud Dumas Chloe Zubieta François Parcy Laboratoire Physiologie Cellulaire et Vegetale Universite Grenoble AlpesCNRSCEAINRAEIRIG-DBSCI-LPCV17 avenue des martyrs38054GrenobleFrance Universite Paris-Saclay CNRSINRAEUniversite d'EvryInstitute of Plant Sciences Paris-Saclay(IPS2)91405OrsayFrance The Bioinformatics Centre Department of Biology and Biotech and Research Innovation CentreUniversity of CopenhagenOle Maalces Vej 5DK2200 Copenhagen NDenmark Laboratoire Reproduction et Developpement des Plantes Universite de LyonENS de LyonUCB Lyon 1CNRSINRA69342 LyonFrance Institut de Biologie Structurale Universite Grenoble AlpesCEACNRSGrenobleFrance Universite de Paris Institute of Plant Sciences Paris-Saclay(IPS2)75006 ParisFrance

Pioneer transcription factors(TFs)are a special category of TFs with the capacity to bind to closed chromatin regions in which DNA is wrapped around histones and may be highly ***,pioneer TFs are able to modify the chromatin state to initiate gene *** plants,LEAFY(LFY)is a master floral regulator and has been suggested to act as a pioneer TF in ***,we demonstrate that LFY is able to bind both methylated and non-methylated DNA using a combination of in vitro genomewide binding experiments and structural *** between regions bound by LFY in vivo and chromatin accessibility data suggest that a subset of LFY bound regions is occupied by *** confirm that LFY is able to bind nucleosomal DNA in vitro using reconstituted ***,we show that constitutive LFY expression in seedling tissues is sufficient to induce chromatin accessibility in the LFY direct target genes APETALA1 and *** together,our study suggests that LFY possesses key pioneer TF features that contribute to launching the floral gene expression program.

关键词： flower development pioneer transcription factor gene regulation chromatin remodeling

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Numerical Investigation of the Influence of a Magnetic Field on the Laminar Flow of a Yield-Stress Nanofluid over a Backward Facing Step

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Frontiers in Heat and Mass Transfer 2025年第1期23卷 185-206页

作者： Karim Amrani eugenia Rossi di Schio Mohamed Bouzit Abderrahim Mokhefi Abdelkader Aris Cherif Belhout Paolo Valdiserri Mechanical Manufacturing Technology Research Laboratory (LaRTFM) National Polytechnic School of Oran Maurice Audin El mnaouar Bp 1523 Es-senia Oran 31000 Algeria Department of Industrial Engineering DIN Alma Mater Studiorum-University of Bologna Viale Risorgimento 2 Bologna 40136 Italy Laboratory of Maritime Sciences and Engineering LSIM Faculty of Mechanical Engineering University of Science and Technology of Oran Mohamed Boudiaf El Mnaouer Oran 31000 Algeria Mechanics Modeling and Experimentation Laboratory L2ME Faculty of Sciences and Technology Bechar University Bechar 08000 Algeria

The present study focuses on the flow of a yield-stress (Bingham) nanofluid, consisting of suspended Fe 3 O 4 nanoparticles, subjected to a magnetic field in a backward-facing step duct (BFS) configuration. The duct is equipped with a cylindrical obstacle, where the lower wall is kept at a constant temperature. The yield-stress nanofluid enters this duct at a cold temperature with fully developed velocity. The aim of the present investigation is to explore the influence of flow velocity (Re = 10 to 200), nanoparticle concentration ( φ = 0 to 0.1), magnetic field intensity (Ha = 0 to 100), and its inclination angle (γ = 0 to 90) and nanofluid yield stress (Bn = 0 to 20) on the thermal and hydrodynamic efficiency inside the backward-facing step. The numerical results have been obtained by resolving the momentum and energy balance equations using the Galerkin finite element method. The obtained results have indicated that an increase in Reynolds number and nanoparticle volume fraction enhances heat transfer. In contrast, a significant reduction is observed with an increase in Hartmann and Bingham numbers, resulting in quasi-immobilization of the fluid under the magnetic influence and radical solidification of this type of fluid, accompanied by the suppression of the vortex zone downstream of the cylindrical obstacle. This study sheds light on the complexity of this magnetically influenced fluid, with potential implications in various engineering and materials science fields.

关键词： Yield-stress nanofluid Bingham nanofluid backward-facing step BFS magnetic field heat transfer hydrodynamics

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美国推出鲜花采后贮存新型解决方案

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中国花卉园艺 2025年第1期 74-76页

作者： eugenia den Ouden 妍然(译) 不详

在全球范围内,包括在美国,鲜切花的市场需求有明显的季节性波动,情人节是玫瑰销售的高峰期,复活节、母亲节和圣诞节.也是鲜花需求旺季。如何满足节日销售旺季的需求,及时提供新鲜的高品质鲜花?这对花卉行业来说是一项挑战。由此,许多延... 详细信息

在全球范围内,包括在美国,鲜切花的市场需求有明显的季节性波动,情人节是玫瑰销售的高峰期,复活节、母亲节和圣诞节.也是鲜花需求旺季。如何满足节日销售旺季的需求,及时提供新鲜的高品质鲜花?这对花卉行业来说是一项挑战。由此,许多延长切花保鲜期的技术应运而生。

关键词：花卉行业切花保鲜鲜切花全球范围鲜花母亲节情人节季节性波动

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A shape shifting organelle: unusual mitochondrial phenotype determined with three-dimensional electron microscopy reconstruction

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Neural Regeneration Research 2016年第6期11卷 900-901页

作者： eugenia Trushina Department of Neurology Mayo Clinic Department of Molecular Pharmacology and Experimental Therapeutics Mayo Clinic

Mitochondria,the powerhouses of the cell,are dynamic organelles that constantly move and change their size and morphology to meet energetic demands of the cell（Wai and Langer,2016）.Recent studies demonstrated that energy production is dependent on the ability of mitochondria to undergo cycles of fission and fusion collectively termed“mitochondrial dynamics”（Youle and van der Bliek,2012）.

关键词： mitochondrial phenotype Langer constantly fission hippocampal neuronal unusual protective deprivation

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Contemporary climate infuence on variability patterns of Anadenanthera colubrina ***,a key species in seasonally dry tropical forests

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Journal of Forestry Research 2022年第1期33卷 89-101页

作者： María Victoria García María eugenia Barrandeguy Kathleen Prinz Genética de Poblaciones y Cuantitativa-Departamento de Genética-Facultad de Ciencias Exactas Químicas y Naturales-Universidad Nacional deMisionesInstituto de Biología Subtropical(UNaM-CONICET)Nodo PosadasFélix de Azara 15523300 PosadasArgentina Consejo Nacional de Investigaciones Científicas Y Técnicas(CONICET) PosadasMisionesArgentina Institute of Systematic Botany With Herbarium Haussknecht and Botanical Gardens Friedrich-Schiller-University Jena07743 JenaGermany

The distribution of many plant species has been shaped by climate changes,and their current phenotypic and genetic variability refect microclimatically suitable *** study relates contemporary climate to variability patterns of phenotypic traits and molecular markers in the Argentinean distribution of Anadenanthera colubrina ***,as well as to identify the most relevant phenotypic trait or molecular marker associated with those *** from four populations in both biogeographic provinces,Paranaense and Yungas,were *** analyses and multiple linear regressions were carried out to determine relationships among phenotypic traits and nuclear microsatellites,respectively,to climatic variables,and to identify the phenotypic traits as well as nuclear microsatellite loci most sensitive to *** and three clusters of individuals were detected based on genetic and phenotypic data,*** clusters based on genetic data refected the biogeographic origin of *** traits were the most relevant indicators of climatic *** microsatellite locus Ac41.1 appeared to be non-neutral presenting a strong correlation with climate variable temperature *** findings show complex patterns of genetic and phenotypic variability in the Argentinean distribution of *** *** related to the present or contemporary climate,and provides an example for an integrative approach to better understand climate impact on contemporary genetic and phenotypic variability in light of global climate change.

关键词： Contemporary climate Curupay Genetic variability Phenotypic variability Seasonally dry tropical forests

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Bred Vectors of the Lorenz63 System

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Advances in Atmospheric Sciences 2015年第11期32卷 1533-1538页

作者： Ying ZHANG Kayo IDE eugenia KALNAY Department of Atmospheric and Oceanic Science University of MarylandCollege Park

The breeding method has been widely used in studies of data assimilation, predictability and instabilities. The bred vectors （BVs）, which are the nonlinear difference between the control and perturbed runs, represent the time-evolving rapidly growing errors in dynamic systems. The Lorenz （1963） model （hereafter Lorenz63 model） has chaotic dynamics similar to weather and climate. This study investigates the features of BVs of the Lorenz63 model and its impact on regime prediction of the Lorenz63 model. The results show that the Lorenz63 model has two different BVs for each breeding cycle, and the two BVs approach being identical when growth rate is high. The duration of the current and next regime is associated with the relative directions between the BV with high growth rate and the model trajectory.

关键词： bred vectors chaos Lorenz63 model regime prediction

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Mitochondrial complex Ⅰ as a therapeutic target for Alzheimer’s disease

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Acta Pharmaceutica Sinica B 2022年第2期12卷 483-495页

作者： eugenia Trushina Sergey Trushin Md Fayad Hasan Department of Neurology Mayo ClinicRochesterMN 55905USA Department of Molecular Pharmacology and Experimental Therapeutics Mayo ClinicRochesterMN 55905USA

Alzheimer’s disease(AD),the most prominent form of dementia in the elderly,has no *** focused on the reduction of amyloid beta or hyperphosphorylated Tau protein have largely failed in clinical *** therapeutic targets and strategies are urgently *** data suggest that in response to environmental stress,mitochondria initiate an integrated stress response(ISR)shown to be beneficial for healthy aging and ***,we review data that implicate mitochondrial electron transport complexes involved in oxidative phosphorylation as a hub for small molecule-targeted therapeutics that could induce beneficial mitochondrial ***,partial inhibition of mitochondrial complex I has been exploited as a novel strategy for multiple human conditions,including AD,with several small molecules being tested in clinical *** discuss current understanding of the molecular mechanisms involved in this counterintuitive *** this strategy has also been shown to enhance health and life span,the development of safe and efficacious complex Ⅰ inhibitors could promote healthy aging,delaying the onset of age-related neurodegenerative diseases.

关键词： Mitochondria Mitochondria signaling ComplexⅠinhibitors Alzheimer’s disease Integrated stress response Neuroprotection Mitochondria targeted therapeutics Healthy aging

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Transcriptional Analysis of Normal Human Fibroblast Responses to Microgravity Stress

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Genomics, Proteomics & Bioinformatics 2008年第1期6卷 29-41页

作者： Yongqing Liu eugenia Wang Department of Biochemistry and Molecular Biology University of Louisville School of Medicine Louisville KY 40202 USA. Current address. James Graham Brown Cancer Center Department of Ophthalmology and Visual SciencesUniversity of Louisville School of Medicine Louisville KY 40202 USA.

To understand the molecular mechanism（s） of how spaceflight affects cellular signaling pathways, quiescent normal human WI-38 fibroblasts were flown on the STS-93 space shuttle mission. Subsequently, RNA samples from the spaceflown and ground-control cells were used to construct two cDNA libraries, which were then processed for suppression subtractive hybridization （SSH） to identify spaceflight-specific gene expression. The SSH data show that key genes related to oxidative stress, DNA repair, and fatty acid oxidation are activated by spaceflight, suggesting the induction of cellular oxidative stress. This is further substantiated by the up-regulation of neuregulin 1 and the calcium-binding protein calmodulin 2. Another obvious stress sign is that spaceflight evokes the Ras/mitogen-activated protein kinase and phosphatidylinositol-3 kinase signaling pathways, along with up-regulating several Gl-phase cell cycle traverse genes. Other genes showing upregulation of expression are involved in protein synthesis and pro-apoptosis, as well as pro-survival. Interactome analysis of functionally related genes shows that c-Myc is the ＂hub＂ for those genes showing significant changes. Hence, our results suggest that microgravity travel may impact changes in gene expression mostly associated with cellular stress signaling, directing cells to either apoptotic death or premature senescence.

关键词： microgravity apoptosis premature senescence oxidative stress subtractive hybridization

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Production of electrical energy from living plants in microbial fuel cells

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Clean Energy 2023年第2期7卷 408-416页

作者： Raymond Daniel Rodriguez Martinez Maria eugenia Alvarez Bermudez Department of Industrial Production Faculty of EngineeringUniversidad Metropolitana1073 CaracasVenezuela Department of Energy and Automation Faculty of EngineeringUniversidad Metropolitana1073 CaracasVenezuela

Growing energy demand is a current problem with greater incidence in the *** research objective is to establish seed bases to give a leading role to plants used in anthropogenic production activities and consumption,and even plants belonging to wildlife in terms of energy *** use of living plants to obtain electrical energy through a microbial-vegetable fuel cell is proposed as a mech-anism to supply the energy demand with easy access worldwide,especially in areas far from urban *** this work,the voltage produced by microbial-vegetable fuel cells was measured using graphite electrodes and plants belonging to the species of aloe(Aloe vera),corn(Zea mays),black beans(Phaseolus vulgaris),tomato(Solanum lycopersicum)and moss(Dicranidae).They were selected for their presence in everyday life and their great commercial and agricultural value,reaching a maximum voltage of 884 mV from *** is evidence that all plants can be a source of energy to a greater or lesser extent and that their performance is given by the plant root interactions at the ***,an extensive root system can favour the accumulation of substrate after the hydrolysis of dead roots,provided that their presence does not interfere with microbial activity on the anodic *** this,using plants with low resistance to thermal stress can lead to a substrate richer in substances that can be ***,it is recommended to use plants according to their life cycle and the morphology of their roots,and plants that can grow in waterlogged conditions or without direct contact with the Sun.

关键词： ecosystems microorganisms renewable energy photosynthesis sustainability

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MULTIOBJECTIVE ROUTING IN MULTISERVICE MPLS NETWORKS WITH TRAFFIC SPLITTING-A NETWORK FLOW APPROACH

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Journal of Systems Science and Systems Engineering 2015年第4期24卷 389-432页

作者： Rita Girao-Silva Jose Craveirinha Joao Climaco M.eugenia Captivo Department of Electrical and Computer Engineering University of Coimbra Polo ⅡPinhal de Marrocos P-3030-290 Coimbra Portugal Institute of Computers and Systems Engineering of Coimbra(INESC-Coimbra)R.Antero de Quental 199 P-3000-033 Coimbra Portugal Centro de Investigacao Operacional Faculdade de CienciasUniversidade de Lisboa Campo GrandeBloco C6Piso 4 P-1749-016 Lisboa Portugal

A multiobjective routing model for Multiprotocol Label Switching networks with multiple service types and traffic splitting is presented in this paper. The routing problem is formulated as a multiobjective mixed-integer program, where the considered objectives are the minimization of the bandwidth routing cost and the minimization of the load cost in the network links with a constraint on the maximal splitting of traffic trunks. Two different exact methods are developed for solving the formulated problem, one based on the classical constraint method and another based on a modified constraint method. A very extensive experimental study, with results on network performance measures in various reference test networks and in randomly generated networks, is also presented and its results are discussed.

关键词： Routing models multiobjective optimization telecommunication networks network flowapproach traffic splitting

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