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Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates

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World Journal of Stem Cells 2021年第5期13卷 452-469页

作者： vladimir P Baklaushev Oleg v Durov vladimir A Kalsin eugene v gulaev Sergey v Kim Ilya L Gubskiy veronika A Revkova Ekaterina M Samoilova Pavel A Melnikov Dzhina D Karal-Ogly Sergey v Orlov Alexander v Troitskiy vladimir P Chekhonin Alexander v Averyanov Jan-Eric Ahlfors Biomedical Research Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBA of RussiaMoscow 115682MoskvaRussia Department of Neurosurgery Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBAMoscow 115682MoskvaRussia Department of Anesthesiology N.N.Blokhin Russian Cancer Research CentreMoscow 115478MoskvaRussia Radiology and Clinical Physiology Scientific Research Center Federal center of brain research and neurotechnologies of the Federal Medical Biological AgencyMoscow 117997Russia Department of Neurobiology Serbsky National Medical Research Center for Psychiatry and NarcologyMoscow 119992MoskvaRussia Department of Primatology Russian Acad Med SciResearch Institute of Medical PrimatologySochi 119992SochiRussia Department of vascular Surgery Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBA of RussiaMoscow 115682MoskvaRussia Department of Basic and Applied Neurobiology V.P.Serbsky Federal Medical Research Center for Psychiatry and NarcologyRussia Department of Medical Nanobiotechnology Pirogov Russian National Research Medical University(RNRMU)Moscow 115682MoskvaRussia New World Laboratories Laval H7V 4A7QuebecCanada

BACKGROUND The development of regenerative therapy for human spinal cord injury(SCI)is dramatically restricted by two main challenges:the need for a safe source of functionally active and reproducible neural stem cells and the need of adequate animal models for preclinical *** reprogramming of somatic cells into neuronal and glial precursors might be a promising solution to the first *** use of non-human primates for preclinical studies exploring new treatment paradigms in SCI results in data with more translational relevance to human *** To investigate the safety and efficacy of intraspinal transplantation of directly reprogrammed neural precursor cells(drNPCs).METHODS Seven non-human primates with verified complete thoracic SCI were divided into two groups:drNPC group(n=4)was subjected to intraspinal transplantation of 5 million drNPCs rostral and caudal to the lesion site 2 wk post injury,and lesion control(n=3)was injected identically with the equivalent volume of *** Follow-up for 12 wk revealed that animals in the drNPC group demonstrated a significant recovery of the paralyzed hindlimb as well as recovery of somatosensory evoked potential and motor evoked potential of injured *** resonance diffusion tensor imaging data confirmed the intraspinal transplantation of drNPCs did not adversely affect the morphology of the central nervous system or cerebrospinal fluid *** immunohistochemical analysis showed that drNPCs maintained SOX2 expression characteristic of multipotency in the transplanted spinal cord for at least 12 wk,migrating to areas of axon growth *** Our data demonstrated that drNPC transplantation was safe and contributed to improvement of spinal cord function after acute SCI,based on neurological status assessment and neurophysiological recovery within 12 wk after *** functional improvement described was not associated with neuronal differentiation of th

关键词： Direct cell reprogramming Neural precursor cells Directly reprogrammed neural precursor cells Spinal cord injury Nonhuman primates Regenerative therapy,Evoked potentials

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Study of Completed Archaeal Genomes and Proteomes:Hypothesis of Strong Mutational AT Pressure Existed in Their Common Predecessor

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Genomics, Proteomics & Bioinformatics 2010年第1期8卷 22-32页

作者： vladislav v.Khrustalev eugene v.Barkovsky Department of General Chemistry Belarussian State Medical University

The number of completely sequenced archaeal genomes has been sufficient for a large-scale bioinformatic study. We have conducted analyses for each coding region from 36 archaeal genomes using the original CGS algorithm by calculating the total GC content （G＋C）, GC content in first, second and third codon positions as well as in fourfold and twofold degenerated sites from third codon positions, levels of arginine codon usage （Arg2： AGA/G; Arg4： CGX）, levels of amino acid usage and the entropy of amino acid content distribution. In archaeal genomes with strong GC pressure, arginine is coded preferably by GC-rich Arg4 codons, whereas in most of archaeal genomes with G＋C〈0.6, arginine is coded preferably by AT-rich Arg2 codons. In the genome of Haloquadratum walsbyi, which is closely related to GC-rich archaea, GC content has decreased mostly in third codon positions, while Arg4〉〉Arg2 bias still persists. Proteomes of archaeal species carry characteristic amino acid biases： levels of isoleucine and lysine are elevated, while levels of alanine, histidine, glutamine and cytosine are relatively decreased. Numerous genomic and proteomic biases observed can be explained by the hypothesis of previously existed strong mutational AT pressure in the common predecessor of all archaea.

关键词： G+C 3GC arginine mutational pressure archaea entropy

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A 96-antenna radioheliograph

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Research in Astronomy and Astrophysics 2014年第7期14卷 864-868页

作者： Sergey v.Lesovoi Alexander T.Altyntsev eugene F.Ivanov Alexey v.Gubin Institute of Solar-Terrestrial Physics

Here we briefly present some design approaches for a multifrequency 96-antenna radioheliograph. The configuration of the array antenna, transmission lines and digital receivers is the main focus of this work. The radioheliograph is a T-shaped centrally condensed radiointerferometer operating in the frequency range 4–8 *** justification for the choice of such a configuration is discussed. The signals from antennas are transmitted to a workroom by analog optical links. The dynamic range and phase errors of the microwave-over-optical signal are considered. The signals after downconverting are processed by digital receivers for delay tracking and fringe stopping. The required step of delay tracking and data rates are considered. Two 3-bit data streams（I and Q） are transmitted to a correlator with the transceivers embedded in Field Programmed Gate Array chips and with PCI Express cables.

关键词： telescopes -- instrumentation: interferometers -- Sun: radio radiation

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Highly selective/enantioselective Pt-ReOx/C catalyst for hydrogenation of L-malic acid at mild conditions

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Journal of Energy Chemistry 2018年第3期27卷 903-912页

作者： Bair S.Bal'zhinimaev eugene A.Paukshtis Alexey P.Suknev Nikita v.Makolkin Boreskov Institute of Catalysis SB RA5 Prospect Akademika Lavrentieva5Novosibirsk 630090Russia

The catalyst preparation strategy was based on a strict introduction sequence of rhenium and platinum precursors and their strong interaction with carbon support resulted in the formation of 0.5 nm Pt-Re Ox species of atomic dispersion, where platinum is metallic, while monolayer rhenium is partially oxidized（Re2＋）. The reaction kinetics was studied taking into account the process of L-malic acid association leading to the formation of inactive cyclic oligomeric species. High TOFs（ca. 50 h-1）, selectivities（ca. 99%）and stability of Pt-Re Ox/C catalysts in aqueous-phase hydrogenation of L-malic acid, which are close to those of the homogeneous pincer type complexes, were revealed at mild conditions（T = 90–130 ℃）. Taking into account that（i） hydrogenation reaction occurred 2–3 orders of magnitude faster than its racemization and（ii） association of L-malic acid dominates at low temperatures and in a concentrated solution,special reaction conditions that allow obtaining chemically and optically（ee 〉 99%） pure（S）-3-hydroxy-γ-butyrolactone and（S）-1,2,4-butanetriol were found. Basing on HAADF-STEM, EDX, XPS, and kinetic studies, the structure of active species and basic reaction pathways are proposed.

关键词： L-malic acid Hydrogenation Enantioselectivity Platinum Rhenium Atomic dispersion

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An Approach for Searching Insertions in Bacterial Genes Leading to the Phase Shift of Triplet Periodicity

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Genomics, Proteomics & Bioinformatics 2011年第4期9卷 158-170页

作者： Maria A. Korotkova Nikolay A. Kudryashov eugene v. Korotkov Centre of Bioengineering Russian Academy of Sciences National University of Nuclear Investigations (MIFI)

The concept of the phase shift of triplet periodicity （TP） was used for searching potential DNA insertions in genes from 17 bacterial genomes. A mathematical algorithm for detection of these insertions has been developed. This approach can detect potential insertions and deletions with lengths that are not multiples of three bases, especially insertions of relatively large DNA fragments （〉 100 bases）. New similarity measure between triplet matrixes was employed to improve the sensitivity for detecting the TP phase shift. Sequences of 17,220 bacterial genes with each consisting of more than 1,200 bases were analyzed, and the presence of a TP phase shift has been shown in -16% of analysed genes （2,809 genes）, which is about 4 times more than that detected in our previous work. We propose that shifts of the TP phase may indicate the shifts of reading frame in genes after insertions of the DNA fragments with lengths that are not multiples of three bases. A relationship between the phase shifts of TP and the frame shifts in genes is discussed.

关键词： triplet periodicity insertion gene sequence reading frame phase shift change-point problem

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Post-Late Paleozoic Collisional Framework of Southern Great Altai

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Acta Geologica Sinica(English Edition) 2012年第5期86卷 1093-1104页

作者： Boris M. CHIKOv Sergei v. ZINOvIEv Еugene v. DEYEv Institute of Geology Siberian Branch of Russian Academy of Sciences Novosibirsk 630090 Russia

We outline the post-Late Paleozoic （latest Permian to Cenozoic） collisional framework of the southern Great Altai （Central Asia） produced by the convergence between the Tuva-Mongolia and Junggar continental terranes （microplates）. The collisional structures in the region classified on the basis of their geometry and deformation style, dynamic metamorphism, and compositions of tectonites are of three main types：（1） mosaic terranes made up of large weakly deformed Paleozoic blocks separated by younger shear zones; （2） contractional deformation systems involving structures formed in post-Late Paleozoic time, parallel faults oriented along collisional deformation systems, and relict lenses of Paleozoic orogenic complexes; and （3） isolated zones of dynamic metamorphism composed mostly of collisional tectonites different in composition and alteration grade.

关键词： continental collision post-Late Paleozoic events tectonic framework dynamic metamorphism Altai Central Asia

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Efficacy and safety of stellate ganglion block in chronic ulcerative colitis

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World Journal of Gastroenterology 2017年第17期23卷 3193-3194页

作者： eugene Lipov Ken Candido department of anesthesiology advocate illinois masonic medical center

Sympathetic system modulation by stellate ganglion blockade may modulate immune dysfunction and significantly improve symptoms of chronic ulcerative colitis.

关键词： Stellate ganglion block Ulcerative colitis Immune function Chronic ulcerative colitis

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On Determining the Optimal Lifting Law of the Walking Propulsion Device Foot of an Underwater Robot from the Bottom

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Journal of Artificial Intelligence and Technology 2021年第4期1卷 214-218页

作者： eugene S.Briskin Yaroslav v.Kalinin Liliya D.Smirnaya Department of Theoretical Mechanics Volgograd State Technical UniversityLenin Avenue28Volgograd 400005Russia Center for Technology Components of Robotics and Mechatronics Innopolis UniversityUniversitetskaya Street1Innopolis 420500Russia

The problem of lifting the foot of the walking propulsion device of an underwater mobile robot is considered,taking into account the additional"compression""force acting on it.A mathematical model has been developed for the detachment of a propulsion foot from the ground,based on Henry's laws establishing the concentration of dissolved air in a liquid,the law of gas expansion at a constant temperature,Darcy's law on fluid filtration,and the theorem on the motion of the center of mass of a solid *** linearized model allows to obtain and analytical *** on the solution of the variat ional problem,optimal modes of lifting the foot of the walking propulsion of an underwater mobile robot are established.

关键词： walking propulsion device underwater walking robot pulling force the force of resistance to motion optimal control

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Evidence for polarization-induced phase transformations and degradation in CH_(3)NH_(3)PbI_(3)

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Nano Research 2023年第7期16卷 9435-9442页

作者： Aleksei Yu.Grishko Maria A.Komkova Ekaterina I.Marchenko Alexandra v.Chumakova Alexey B.Tarasov eugene A.Goodilin Andrei A.Eliseev Department of Materials Science MSU-BIT UniversityShenzhen 517182China Department of Chemistry Lomonosov Moscow State UniversityMoscow 119991Russia Department of Materials Science Lomonosov Moscow State UniversityMoscow 119991Russia European Synchrotron Radiation Facility F-38042 GrenobleFrance

In solar cells,hybrid halide perovskites operate under constant bias,thus their stability towards electric field-induced degradation is of key *** we report on evidence of previously unidentified electric field-induced transitions and degradation path of CH_(3)NH_(3)PbI_(3)(MAPbI_(3))using elemental and phase *** films of MAPbI_(3) were deposited onto 1-2μm-pitch interdigitated electrodes and subjected to direct current(DC)-*** MAPbI_(3) layer polarized withv/μm DC electric field undergoes pronounced ion redistribution to methylammonium-rich MAPbI_(3)-y(yvokes degradation of MAPbI_(3).Using nanofocus grazing-incidence wide-angle X-ray scattering(GIWAXS),we unambiguously showed that the bias voltage induces the transformation ofβ-MAPbI_(3) to metastableδ-MAPbI_(3) polymorph via alignment of polar organic cation with the electric *** transformation is partially reversible upon field ***,once formed,δ-MAPbI_(3) disrupts the morphology of pristine film and undergoes decomposition toβ-MAPbI_(3)(β-MAPI)and PbI_(2).With the aforementioned compositional and phase changes,only MA-rich part serves as the charge separation layer,while the I-rich excitation is blocked with the PbI_(2) barrier serving as holes trapping *** observations reveal the intermediate steps in electric-field-driven degradation of halide perovskites and show the role of polar cations in the process,which is instructive for further material design with higher stability metrics.

关键词： halide perovskites stability degradation electric field ionic conductivity stoichiometry

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Recent advances in mass spectrometry-based proteomics of gastric cancer

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World Journal of Gastroenterology 2016年第37期22卷 8283-8293页

作者： Changwon Kang Yejin Lee J eugene Lee Department of Biological Sciences Korea Advanced Institute of Science and Technology Center for Bioanalysis Korea Research Institute of Standards and Science

The last decade has witnessed remarkable technological advances in mass spectrometry-based proteomics. The development of proteomics techniques has enabled the reliable analysis of complex proteomes, leading to the identification and quantification of thousands of proteins in gastric cancer cells, tissues, and sera. This quantitative information has been used to profile the anomalies in gastric cancer and provide insights into the pathogenic mechanism of the disease. In this review, we mainly focus on the advances in mass spectrometry and quantitative proteomics that were achieved in the last five years and how these up-andcoming technologies are employed to track biochemical changes in gastric cancer cells. We conclude by presenting a perspective on quantitative proteomics and its future applications in the clinic and translational gastric cancer research.

关键词： Gastric cancer Mass spectrometry Protein identification Proteomics Protein quantification

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