检索结果-南通市图书馆

In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes:two different manufacturing processes for the treatment of deep and difficult to heal injuries

引用

Burns & Trauma 2023年第1期11卷 800-813页

作者： Álvaro Sierra-Sánchez Brice Magne etienne savard Christian Martel Karel Ferland Martin A.Barbier Anabelle Demers Danielle Larouche Salvador Arias-Santiago Lucie Germain LOEX Tissue Engineering Laboratory and Department of Surgery Faculty of MedicineUniversitéLaval140118e rueQuébec(Québec)G1J 1Z4Canada CHU de Québec-UniversitéLaval Research Center Division of Regenerative Medicine140118e rueQuébec(Québec)G1J 1Z4Canada Unidad de Producción Celular e Ingeniería Tisular(UPCIT) Virgen de las Nieves University Hospitalibs.GRANADAAndalusian Network for the design and translation of Advanced TherapiesAv.de las Fuerzas ArmadasNº24ªPlanta Ed.de Gobierno18014GranadaSpain Department of Dermatology Virgen de las Nieves University HospitalAv.MadridNº11-1518012GranadaSpain Department of Dermatology Faculty of MedicineUniversity of GranadaAv.de la InvestigaciónNº1118016GranadaSpain

Background:The aim of this in vitro study was to compare side-by-side two models of human bilayered tissue-engineered skin substitutes(hbTESSs)designed for the treatment of severely burned *** are the scaffold-free self-assembled skin substitute(SASS)and the human plasma-based skin substitute(HPSS).Methods:Fibroblasts and keratinocytes from three humans were extracted from skin biopsies(N=3)and cells from the same donor were used to produce both hbTESS *** SASS manu-facture,keratinocytes were seeded over three self-assembled dermal sheets comprising fibroblasts and the extracellular matrix they produced(n=12),while for HPSS production,keratinocytes were cultured over hydrogels composed of fibroblasts embedded in either plasma as unique biomaterial(Fibrin),plasma combined with hyaluronic acid(Fibrin-HA)or plasma combined with collagen(Fibrin-Col)(n/biomaterial=9).The production time was 46-55 days for SASSs and 32-39 days for *** were characterized by histology,mechanical testing,PrestoBlue™-assay,immunofluorescence(Ki67,Keratin(K)10,K15,K19,Loricrin,type IV collagen)and Western blot(type I and IV collagens).Results:The SASSs were more resistant to tensile forces(p-value<0.01)but less elastic(p-value<0.001)compared to HPSSs.A higher number of proliferative Ki67+cells were found in SASSs although their metabolic activity was *** epidermal differentiation,no significant difference was observed in the expression of K10,K15,K19 and ***,the production of type I and type IV collagens and the adhesive strength of the dermal-epidermal junction was higher in ***:This study demonstrates,for the first time,that both hbTESS models present similar in vitro biological ***,mechanical properties differ and future in vivo experiments will aim to compare their wound healing potential.

关键词： Biomaterial Burn Fibrin Human plasma Self-assembly Skin substitute Tissue engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：