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Fluid Flow Estimation with multiscale Ensemble Filters Based on motion measurements Under Location Uncertainty

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Numerical mathematics(Theory,methods and Applications) 2013年第1期6卷 21-46页

作者： Sébastien Beyou Thomas Corpetti Sai Gorthi etienne mémin INRIA/FLUmINANCE 35042 Rennes CedexFrance CNRS/LETG-Rennes-Costel Campus Villejean Place du recteur Henri Le Moal CS *** Rennes cedexFrance Indian Institute of Space Science and Technology Valiamala P.O.Thiruvananthapuram–695547Kerala India

This paper proposes a novel multi-scale fluid flow data assimilation approach,which integrates and complements the advantages of a Bayesian sequential assimilationtechnique, the Weighted Ensemble Kalman filter (WEnKF) [27]. The data assimilation proposed in this work incorporates measurement brought by an efficient multiscalestochastic formulation of the well-known Lucas-Kanade (LK) estimator. This estimatorhas the great advantage to provide uncertainties associated to the motion measurements at different scales. The proposed assimilation scheme benefits from this multiscale uncertainty information and enables to enforce a physically plausible dynamicalconsistency of the estimated motion fields along the image sequence. Experimentalevaluations are presented on synthetic and real fluid flow sequences.

关键词： Data assimilation stochastic filter particle filters fluid motion estimation

Cytokinome of adult-derived human liver stem/progenitor cells:immunological and inflammatory features

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Hepatobiliary Surgery and Nutrition 2018年第5期7卷 331-344页

作者： mehdi Najar Emerence Crompot Gordana Raicevic etienne m.Sokal mustapha Najimi Laurence Lagneaux Laboratory of Clinical Cell Therapy Jules Bordet InstituteUniversitéLibre de Bruxelles(ULB)Campus ErasmeBatiment de Transfusion(Level+1)Brussels 1070Belgium Laboratory of Pediatric Hepatology and Cell Therapy Institute of Experimental&Clinical ResearchUniversitéCatholique de LouvainBrusselsBelgium

Background: Being non-immunogenic and capable of achieving major metabolic liver functions, adult-derived human liver stem/progenitor cells (ADHLSCs) are of special interest in the field of liver cell therapy. The cytokine repertoire of engrafted cells may have critical impacts on the immune response balance, particularly during cell transplantation. methods: In this work, we analyzed the cytokinome of ADHLSCs during hepatogenic differentiation (HD) following stimulation with a mixture of inflammatory cytokines (I) in vitro and compared it to that of mature hepatocytes. Results: Independent of their hepatic state, ADHLSCs showed no constitutive expression of pro-inflammatory cytokines, which were significantly induced by inflammation (IL-1β, IL-6, IL-8, TNFα, CCL5, IL-12a, IL-12b, IL-23p19, IL-27p28 and EBI-3). IL1-RA and IDO-1, as immunoregulatory cytokines, were highly induced in undifferentiated ADHLSCs, whereas TGF-β was downregulated by both hepatic and inflammatory events. Interestingly, TDO-1 was exclusively expressed in ADHLSCs after hepatic differentiation and enhanced by inflammatory cytokines. Compared to mature hepatocytes, hepatic-differentiated ADHLSCs showed significantly different cytokine expression patterns. Conclusions: By establishing the cytokinome of ADHLSCs and highlighting their immunological and inflammatory features, we can enhance our knowledge about the safety and efficiency of the transplantation strategy.

关键词： Immunology adult-derived human liver stem/progenitor cells (ADHLSCs) mesenchymal cells cytokinome gene expression analysis

Epithelial cells with hepatobiliary phenotype:Is it another stem cell candidate for healthy adult human liver?

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World Journal of Gastroenterology 2007年第10期13卷 1554-1560页

作者： Dung Ngoc Khuu mustapha Najimi etienne m Sokal Université Catholique de Louvain Laboratory of Pediatric Hepatology & Cell therapyBrusselsBelgium

AIm： To investigate the presence and role of liver epithelial cells in the healthy human adult liver. mETHODS： Fifteen days after human hepatocyte primary culture, epithelial like cells emerged and started proliferating. Cell colonies were isolated and sub- cultured for more than 160 d under specific culture conditions. Cells were analyzed for each passage using immunofluorescence, flow cytometry and reverse transcription-polymerase chain reaction （RT-PCR）. RESULTS： Flow cytometry analysis demonstrated that liver epithelial cells expressed common markers for hepatic and stem cells such as CD90, CD44 and CD29 but were negative for CD34 and CDl17. Using immunofluorescence we demonstrated that liver epithelial cells expressed not only immature （α-fetoprotein） but also differentiated hepatocyte （albumin and CK-18） and biliary markers （CK-7 and 19）, whereas they were negative for OV-6. RT-PCR analysis confirmed immunofluorescence data and revealed that liver epithelial cells did not express mature hepatocyte markers such as CYP2B6, CYP3A4 and tyrosine amino-transferase. Purified liver epithelial cells were transplanted into SCID mice. One month after transplantation, albumin positive cell foci were detected in the recipient mouse parenchyma. CONCLUSION： According to their immature and bipotential phenotype, liver epithelial cells might represent a pool of precursors in the healthy human adult liver other than oval cells.

关键词： Epithelial-like cells Liver stem cell Hepatocyte Differentiation Cell therapy

Stem cells for liver tissue repair:Current knowledge and perspectives

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World Journal of Gastroenterology 2008年第6期14卷 864-875页

作者： Philippe A Lysy David Campard Fran oise Smets mustapha Najimi etienne m Sokal Université Catholique de Louvain & Cliniques Universitaires Saint Luc HPED Department Unit PEDI Laboratory of Pediatric Hepatology and Cell Therapy Brussels 1200 Belgium

Stem cells from extra-or intrahepatic sources have been recently characterized and their usefulness for the generation of hepatocyte-like lineages has been demonstrated. Therefore, they are being increasingly considered for future applications in liver cell therapy. In that field, liver cell transplantation is currently regarded as a possible alternative to whole organ transplantation, while stem cells possess theoretical advantages on hepatocytes as they display higher in vitro culture performances and could be used in autologous transplant procedures. However, the current research on the hepatic fate of stem cells is still facing difficulties to demonstrate the acquisition of a full mature hepatocyte phenotype, both in vitro and in vivo. Furthermore, the lack of obvious demonstration of in vivo hepatocyte-like cell functionality remains associated to low repopulation rates obtained after current transplantation procedures. The present review focuses on the current knowledge of the stem cell potential for liver therapy. We discuss the characteristics of the principal cell candidates and the methods to demonstrate their hepatic potential in vitro and in vivo. We finally address the question of the future clinical applications of stem cells for liver tissue repair and the technical aspects that remain to be investigated.

关键词： Stem cells Hepatocyte differentiation Liver regeneration Cell therapy

Liver cell transplantation for Crigler-Najjar syndrome type Ⅰ: Update and perspectives

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World Journal of Gastroenterology 2008年第22期14卷 3464-3470页

作者： Philippe A Lysy mustapha Najimi Xavier Stéphenne Annick Bourgois Franoise Smets etienne m Sokal Université Catholique de Louvain Cliniques Universitaires Saint Luc HPED Department PEDI Unit Laboratory of Pediatric Hepatology and Cell Therapy Brussels B-1200 Belgium

Liver cell transplantation is an attractive technique to treat liver-based inborn errors of metabolism. The feasibility and efficacy of the procedure has been demonstrated, leading to medium term partial metabolic control of various diseases. Crigler-Najjar is the paradigm of such diseases in that the host liver is lacking one function with an otherwise normal parenchyma. The patient is at permanent risk for irreversible brain damage. The goal of liver cell transplantation is to reduce serum bilirubin levels within safe limits and to alleviate phototherapy requirements to improve quality of life. Preliminary data on Gunn rats, the rodent model of the disease, were encouraging and have led to successful clinical trials. Herein we report on two additional patients and describe the current limits of the technique in terms of durability of the response as compared to alternative therapeutic procedures. We discuss the future developments of the technique and new emerging perspectives.

关键词： Hepatocyte transplantation Cell therapy Inborn error of metabolism Crigler-Najjar Liver regeneration Animal models

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母猪对高纤维饲料和禾谷类饲料的利用

中国畜牧兽医 1988年第1期 47-49页

作者： m·etienne 曹光辛

母猪利用高纤维饲料,主要是在能量需要量较低的妊娠期。在妊娠日粮中,采用高水平的纤维饲料如苜蓿干草或苜蓿草粉、粉碎的稿秆或次麦粉,母猪的能量摄入量降低,因而母猪的增重和有时仔猪初生重下降。这些饲料的能量和粗纤维含量,对受胎...

母猪利用高纤维饲料,主要是在能量需要量较低的妊娠期。在妊娠日粮中,采用高水平的纤维饲料如苜蓿干草或苜蓿草粉、粉碎的稿秆或次麦粉,母猪的能量摄入量降低,因而母猪的增重和有时仔猪初生重下降。这些饲料的能量和粗纤维含量,对受胎率和产仔数没有影响。泌乳期日粮要避免高纤维饲料,因为它们对母猪的体重变化及仔猪的生长和成活力均起不良作用。妊娠期饲料供给量往往限制在自由采食量的50％左右,在妊娠日粮中添加大容积饲料,使母猪有饱感,更加安静,而营养物质的摄入量并未超过。为预防便秘和乳房炎子宫炎无乳综合症,在分娩前后有时也考虑利用纤维日粮。一些研究表明,高纤维大容积饲料对母猪的营养价值较生长猪高。本文着重综述母猪对高纤维饲料细胞壁成分和能量的利用,饲料的食入量、消化利用率,能量代谢和净利用方面的差异,以及对繁殖性能影响的研究结果。此外,还考虑母猪对禾谷类饲料能量的消化利用问题。

关键词：纤维饲料泌乳期仔猪饲粮妊娠期母猪妊娠能量消化率繁殖性能生产性能禾谷类

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繁殖母猪的能量和蛋白质营养(续完)

国外畜牧学（猪与禽） 2001年第6期21卷 18-21页

作者： J.Noblet J.Y.Dourmad m.etienne 法国国家农业科学院

泌乳母猪的营养能量需要量和妊娠母猪一样,用于估计泌乳母猪能量需要量的基本数据见表1.显而易见,最重要的变异因子是母猪的产奶量水平.母猪的产奶量很难测定,通常是通过窝增重来进行估计(Noblet和etienne,1989;表1).引起泌乳母猪奶产... 详细信息

泌乳母猪的营养能量需要量和妊娠母猪一样,用于估计泌乳母猪能量需要量的基本数据见表1.显而易见,最重要的变异因子是母猪的产奶量水平.母猪的产奶量很难测定,通常是通过窝增重来进行估计(Noblet和etienne,1989;表1).引起泌乳母猪奶产量及进而引起泌乳母猪的能量需要量发生变化的因素可见etienne等(1999)及Noblet(1999)的综述.图2所示为能量需要量随窝重增长而发生的变化.窝增重从每天2000克增加到每天3000克,母猪代谢能需要量将从每天80兆焦增至每天105兆焦,或者说相当于常规日粮从每天6.5千克增加到每天8.5千克.

关键词：繁殖母猪能量蛋白质营养

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繁殖母猪的能量和蛋白质营养

国外畜牧学（猪与禽） 2001年第5期21卷 15-18页

作者： J.Noblet J.Y.Dourmad m.etienne 法国国家农业科学院

前言研究报道表明,母猪的利用年限与其生产率在逻辑上呈正相关,其利用年限及其有关的问题(返情率,受胎率)一方面取决于母猪的整个繁殖周期中躯体组成变化的特点,更重要的是取决于泌乳期间母猪瘦肉和(或)脂肪组织的减少量(King,1987;Whit...

前言研究报道表明,母猪的利用年限与其生产率在逻辑上呈正相关,其利用年限及其有关的问题(返情率,受胎率)一方面取决于母猪的整个繁殖周期中躯体组成变化的特点,更重要的是取决于泌乳期间母猪瘦肉和(或)脂肪组织的减少量(King,1987;Whittemore和morgan,1990).为达到最高繁殖性能的最佳饲养方案,是使母猪躯体成分在繁殖周期任何阶段的变化减少到最低限度(Dourmad等,1994).这样的方案要求有充足的饲料供应以满足整个繁殖周期母猪营养需要.本文目的在于对妊娠及泌乳母猪的能量与蛋白质营养方面作一综述,同时提供一些在实际条件下预测营养需要量的原则,本文将讨论使用析因法进行预测.本文还将探讨气候环境(如环境温度)对泌乳母猪的能量需要量,尤其是对采食量的影响.

关键词：生长猪能量需要量日粮纤维成年母猪经产母猪能量消化率妊娠母猪怀孕母猪代谢能生物能非必需氨基酸理想蛋白质兆焦赖氨酸水平可消化赖氨酸消化能增重子宫组织营养

Hydrolytic and Antimicrobial Activities of Bacteria Isolated from Fermented Peppers Sold in markets at Brazzaville, Republic of the Congo

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Journal of Biosciences and medicines 2024年第9期12卷 215-234页

作者： Faly Armel Soloka mabika Elgie Viennechie Gatsé Ngo Itsouhou Tarcisse Baloki Ngoulou mérite Berthelie mouanda Nzoussi Nguimbi etienne Laboratory of Cell and molecular Biology Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of the Congo Unit of molecular microbiology and Bioinformatics Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of the Congo

microorganisms are omnipresent in all environments and play mainly the role of transformers, thanks to the multiple enzymes they are able to produce. In order to valorize fermented foods in the Republic of the Congo, this work aimed to characterize and study some properties of microorganisms isolated from samples of peppers sold in three markets of Brazzaville. A numeration of the total aerobic mesophilic flora (TAmF) was made in a solid medium, allowing the evaluation of each sample’s microbial concentration. The microbial mass varied from 2.8 × 105 CFU/g for the Ouénzé sample to 1.8 × 104 CFU/g for the Total sample and 2 × 104 CFU/g for the moungali market sample. The evaluation of the enzymatic properties of the Bacillus isolates showed that 68.42% were capable of producing cellulases and 78.94% were capable of producing amylases and proteases. Antimicrobial activities revealed that 63.15% of the isolates were able to secrete inhibitory substances against E. coli and Staphylococcus aureus. molecular analysis by PCR amplification, sequencing of the 16S rRNA gene and BLAST bioinformatics analysis provides newly identified bacteria strains with new accession numbers in GenBank: Bacillus thuringiensis mBCBR322 (OP474008), Bacillus megaterium mBCBJ1822 (OP476493), Bacillus thuringiensis mBCBR222 (OP476494), Priestia megaterium mBCBJ2022 (OP476495) and Lactobacillus paraplantarum mBCBR1522 (OP476496). multiple sequences alignment of identified sequences with their homologs of GenBank has shown high similarities. The phylogenetic inference assay has provided the two groups of strains observed in this study, and the two groups are very coherent with the phylogeny of the reference.

关键词： Antimicrobial Activity Hydrolytic Activity Sequencing Pepper Brazzaville