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Development of Biotechnology for Microbial Synthesis of Gold and Silver Nanoparticles

Journal of Life Sciences 2013年第2期7卷 110-122页

作者： Tamaz Levan Kalabegishvili Ivane Giorgi Murusidze Elena Ivan Kirkesali Alexander nikoloz Rcheulishvil eteri nikoloz ginturi eteri Simon Gelagutashvili Nana Eremey Kuchava Nanuli Vakhtang Bagdavadze Dodo Trofim Pataraya Manana Amiran Gurielidze Hoi-Ying Holman Marina Vladimirovna Frontasyeva Inga Ivanovna Zinicovscaia Sergey Sergeevich Pavlov Vasiliy Timofeevich Gritsyna Department of Biological System Physics E. Andronikashvili Institute of Physics Javakhishvili State University Tbilisi O177Georgia . Ilia State University Institute of Applied Physics Tbilisi 0162 Georgia S. Durmishidze Institute of Biochemistry and Biotechnology of N.L.E. Georgian Agrarian University Tbilisi 0159 Georgia Department of Ecology Center for Environmental Biotechnology Lawrence Berkeley National Laboratory Berkeley 94720United States Department of Nuclear Physics Joint Institute for Nuclear Research Dubna 141980 Russia Departement of Solid State Physics V.N. Karazin Kharkiv National University Kharkiv 01077 Ukraine

Several bacterial strains of Actinomycetes belonging to Streptomyces and Arthrobacter genera for the first time were used to study the biotechnology of synthesis of gold and silver nanoparticles. The experimental conditions of gold and silver nanoparticles production by the cells of studied strains in aqueous chloroauric acid （HAuCIq） and in silver nitrate （AgNO3） solutions, respectively, were determined. Concentration and time-dependences of nanoparticle formation were investigated. The complex of optical and analytical methods was used for testing the gold and silver nanoparticles in the bacterial biomass. The TEM （Transmission Electron Microscopy） and XRD （X-ray Diffraction） data in all cases demonstrated the presence of crystals with fcc （face centered cubic） structure. The results obtained show that the Actinomycetes are capable of producing gold and silver nanoparticles of spherical shape extracellularly when exposed to suitable compounds. The particle size distribution shows that the sizes of nanoparticles are in the range of 5 nm to 80 nm. The biomass obtained may be used for industrial as well as medical and pharmaceutical purposes.

关键词： Microbial synthesis nanoparticles gold silver biotechnology.

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Synthesis of Gold Nanoparticles by Some Strains of Arthrobacter Genera

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材料科学与工程（中英文A版） 2012年第2期2卷 164-173页

作者： Tamaz Levan Kalabegishvi｜i Elena Ivan Kirkesalt Alexander nikoloz Rcheulishvili Etery nikoloz ginturi Ivane Giorgi Murusidze Dodo Trofim Pataraya Manana Amiran Gurielidze Giorgi Ilia Tsertsvadze Vakhtang nikoloz Gabunia Levan Giorgi Lomidze David Nugzar Gvarialadze Marina VladimirovnaFrontasyeva Sergey Sergeevich Pavlov Inga Ivanovna Zinicovscaia Marianne Janette Raven Ntsoaki Maditselana Francinah Seaga Arnaud Faanhof E. Andronikashvili Institute of Physics I. Javakhishvili State University 6 Tamarashvili str. Tbilisi O177 Georgia llia State University 3/5 K. Cholokashvili Ave. Tbilisi 0162 Georgia Durmishidze Institute of Biochemistry and Biotechnology Georgian Agrarian University D. Agmashenebeli Kheivani 10 km0159 Tbilisi Georgia P. Melikishvili Institute of Physical and Organic Chemistry I. Javakhishvili State University 5 Politkovskaya str. 0186 TbilisiGeorgia Georgian Technical University 77 Kostava Str. Tbilisi O175 Georgia Joint Institute for Nuclear Research 6 Joliot-Curie Str. 1419890 Dubna Russia Nuclear Energy Corporation of South Africa （NECSA） Pelindaba Pretoria South Africa North-West University （Mafikeng Campus） Private Bag X2046 Mmabatho South Africa

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