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Variation in floral form of CRISPR knock-outs of the poplar homologs of LEAFY and AGAMOUS after FT heat-induced early flowering

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Horticulture Research 2023年第8期10卷 183-193页

作者： Amy L.Klocko estefania elorriaga Cathleen Ma Steven H.Strauss Department of Biology University of Colorado Colorado SpringsColorado SpringsCO 80918USA Department of Forest Ecosystems and Society Oregon State UniversityCorvallisOR 97331USA

Plant migration and gene flow from genetically modified or exotic trees to nearby lands or by crossing with wild relatives is a major public and regulatory *** genetic strategies exist to mitigate potential gene flow;however,the long delay in onset of flow-ering is a severe constraint to research *** used heat-induced FT overexpression to speed assessment of the expected floral phenotypes after CRISPR knockout of poplar homologs of the key floral genes,LEAFY and *** selected events with previously characterized CRISPR-Cas9 induced biallelic changes then re-transformed them with the Arabidopsis thaliana FLOWERING LOCUS T(AtFT)gene under control of either a strong constitutive promoter or a heat-inducible *** successfully obtained flowering in both a male and female clone of poplar,observing a wide range of inflorescence and floral forms among flowers,ramets,and insertion ***,flowers obtained from the selected LFY and AG targeted events were consistent with what would be predicted for loss-of-function of these ***-targeted events showed small catkins with leaf-like organs,AG-targeted events had nested floral organs consistent with reduction in floral determinacy and absence of well-formed carpels or *** findings demonstrate the great developmental plasticity of Populus flowers during genetically accelerated flowering,which may be of horticultural *** also provide an informative early view of floral phenotypes and apparent sterility from knockouts of both these gene targets.

关键词： AGAMOUS flowers organs

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Plant Promoters and Terminators for High-Precision Bioengineering

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BioDesign Research 2023年第1期5卷 86-105页

作者： Emily G.Brooks estefania elorriaga Yang Liu James R.Duduit Guoliang Yuan Chung-Jui Tsai Gerald A.Tuskan Thomas G.Ranney Xiaohan Yang Wusheng Liu Department of Horticultural Science North Carolina State UniversityRaleighNC 27607USA. Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA. The Center for Bioenergy Innovation Oak Ridge National LaboratoryOak RidgeTN 37831USA. Warnell School of Forestry and Natural Resource University of GeorgiaAthensGA 30602USA. Department of Plant Biology University of GeorgiaAthensGA 30602USA. Department of Genetics University of GeorgiaAthensGA 30602USA. Mountain Crop Improvement Lab Department of Horticultural ScienceMountain Horticultural Crops Research and Extension CenterNorth Carolina State UniversityMills RiverNC 28759USA.

High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional and posttranscriptional *** transcription is tightly regulated by promoters and *** determine the timing,tissues and cells,and levels of the expression of *** mediate transcription termination of genes and affect mRNA levels posttranscriptionally,e.g.,the 3′-end processing,stability,translation efficiency,and nuclear to cytoplasmic export of *** promoter and terminator combination affects gene *** the present article,we review the function and features of plant core promoters,proximal and distal promoters,and terminators,and their effects on and benchmarking strategies for regulating gene expression.

关键词： expression Promoter proximal

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