检索结果-南通市图书馆

Apolipoprotein B100 is required for hepatitis C infectivity and Mipomersen inhibits hepatitis C

World Journal of Gastroenterology 2016年第45期22卷 9954-9965页

作者： esperance ak schaefer James Meixiong Christina Mark Amy Deik Daniel L Motola Dahlene Fusco Andrew Yang Cynthia Brisac Shadi Salloum Wenyu Lin Clary B Clish Lee F Peng Raymond T Chung Gastrointestinal Unit Massachusetts General Hospital Bossston MA 02114 United States Department of Medicine Harvard Medical School Bossston MA 02114 United States both of the Broad Institute of Harvard and M.I.T. Cambridge MA 02115 United States Temple University Health System Cambridge MA 02142 United States

AIM To characterize the role of apolipoprotein B100(apoB 100) in hepatitis C viral(HCV) infection. METHODS In this study, we utilize a gene editing tool, transcription activator-like effector nucleases(TALENs), to generate human hepatoma cells with a stable genetic deletion of APOB to assess of apoB in HCV. Using infectious cell culture-competent HCV, viral pseudoparticles, replicon models, and lipidomic analysis we determined the contribution of apoB to each step of the viral lifecycle. We further studied the effect of mipomersen, an FDAapproved antisense inhibitor of apoB 100, on HCV using in vitro cell-culture competent HCV and determined itsimpact on viral infectivity with the TCID50 method. RESULTS We found that apo B100 is indispensable for HCV infection. Using the JFH-1 fully infectious cell-culture competent virus in Huh 7 hepatoma cells with TALENmediated gene deletion of apoB(APOB KO), we found a significant reduction in HCV RNA and protein levels following infection. Pseudoparticle and replicon models demonstrated that apo B did not play a role in HCV entry or replication. However, the virus produced by APOB KO cells had significantly diminished infectivity as measured by the TCID-50 method compared to wildtype virus. Lipidomic analysis demonstrated that these virions have a fundamentally altered lipidome, with complete depletion of cholesterol esters. We further demonstrate that inhibition of apoB using mipomersen, an FDA-approved anti-sense oligonucleotide, results in a potent anti-HCV effect and significantly reduces the infectivity of the virus. CONCLUSION Apo B is required for the generation of fully infectious HCV virions, and inhibition of apo B with mipomersen blocks HCV. Targeting lipid metabolic pathways to impair viral infectivity represents a novel host targeted strategy to inhibit HCV.

关键词： Apolipoprotein Lipid Hepatitis C virus Gene silencing Viral replication

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatitis B virus taxonomy and hepatitis B virus genotypes

引用

World Journal of Gastroenterology 2007年第1期13卷 14-21页

作者： Stephan schaefer Abteilung für Virologie Institut für Medizini-sche MikrobiologieVirologie und HygieneUniversitt RostockSchillingallee 70D-18057 RostockGermany

Hepatitis B virus(HBV) is a member of the hepadnavirus *** can be found in both mammals(orthohepadnaviruses) and birds(avihepadnaviruses).The genetic variability of HBV is very *** are eight genotypes of HBV and three clades of HBV isolates from apes that appear to be additional genotypes of *** genotypes are now divided into subgenotypes with distinct virological and epidemiological *** addition,recombination among HBV genotypes increases the variability of *** review summarises current knowledge of the epidemiology of genetic variability in hepadnaviruses and,due to rapid progress in the field,updates several recent reviews on HBV genotypes and subgenotypes.

关键词： Orthohepadnavirus Avihepadnavirus Hepatitis B virus Genotype, Subgenotype Recombination

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Development of a web-platform for mining applications

引用

International Journal of Mining Science and Technology 2018年第1期28卷 95-99页

作者： Christopher Newman Zach Agioutantis Nathaniel schaefer Department of Mining Engineering University of Kentucky

‘‘Web ground control"(web GC) provides users with instantaneous access to mine design applications anywhere, at any time, through a web *** a web-based multiple-tier architecture, users are able to easily access ground control designs, perform on-demand calculations in the field, as well as facilitate project collaborations across multiple users, devices, and operating ***, the web GC platform contains five ground control related design applications previously developed and distributed by the US National Institute of Occupational Safety and Health(NIOSH), that is, analysis of roof bolt stability(ARBS), analysis of longwall pillar stability(ALPS), analysis of retreat mining stability(ARMPS), analysis of retreat mining stability–highwall mining(ARMPS-HWM), and analysis of horizontal stress in mining(AHSM).With respect to design decisions made by the web GC development team, the web GC platform will be able to further integrate future mine design applications providing the mining industry with one of a kind umbrella suite of ground control related software available at ones *** following paper provides a detailed overview on the current state of the web GC platform with discussions ranging from back-end database development and design to the front-end user-platform *** on current progress in platform development as well as beta testing results, the web GC platform is scheduled for release in the fall of 2018.

关键词： Ground control Stability Web application Cloud computing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

迎接新星的闪亮回归

引用

中国国家天文 2024年第9期18卷 68-75页

作者：布拉德利·谢弗宋乔(译) 美国路易斯安那州立大学不详

再发新星北冕座T上一次爆发是在1946年,它将于今年再次闪耀。在未来几个月,你可能将会看到一生中最明亮的一次新星闪耀。这颗恒星就是北冕座T星(T Coronae Borealis,T CrB)。它最近一次爆发是在1946年,如果预测是对的,那么这颗所谓的“... 详细信息

再发新星北冕座T上一次爆发是在1946年,它将于今年再次闪耀。在未来几个月,你可能将会看到一生中最明亮的一次新星闪耀。这颗恒星就是北冕座T星(T Coronae Borealis,T CrB)。它最近一次爆发是在1946年,如果预测是对的,那么这颗所谓的“闪耀星”将很快会为北天的“皇冠”增加一颗壮观的临时“宝石”。

关键词：北冕座再发新星宝石恒星

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Variations in net litter nutrient input associated with tree species influence on soil nutrient contents in a subtropical evergreen broad-leaved forest

引用

Chinese Science Bulletin 2014年第1期59卷 46-53页

作者： Lu Qiao Doug Allen schaefer Xiaoming Zou Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences University of Chinese Academy of Sciences Department of Environmental Studies University of Puerto Rico

The influence of tree species on soils has received much attention,but we know little about either the pattern and underlying mechanisms of net nutrient and carbon inputs under canopy for various tree species or the effects of net nutrient input on soil nutrient *** address these issues,we selected three tree species with distinct leaf sizes and arranged litter traps around the target individuals to test what affected net nutrient and carbon inputs under canopy of target individuals,and how net nutrient inputs influenced soil nutrient *** results showed that net litter manganese(Mn)and lignin inputs were significantly different among these tree species,as well as soil exchangeable potassium(K),available iron(Fe)and available *** results also revealed that the species with the smallest sized leaves may stimulate more net Mn and lignin inputs than the species with the largest sized *** the same time,net nutrient inputs correlated with soil available Fe,while the ratio of lignin to nitrogen of net litter correlated with total phosphorus *** results demonstrated that litter production may be the main factor deciding net nutrient and carbon inputs under different tree species’canopy,which significantly differentiated soil iron’s availability.

关键词：土壤养分含量亚热带常绿阔叶林树种枯落物输入营养养分投入木质素

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of micro-dimple patterns on capillary pull-off force and friction force of silicon surface

引用

Chinese Physics B 2009年第1期18卷 231-237页

作者：张向军董云开刘永合 J.A.schaefer State Key Laboratory of Tribology Tsinghua University Institute of Physics and Center of Micro/nano Technology Technich University of IlmenauPF 100565Germany

A microtribometer is used to measure and compare pull-off forces and friction forces exerted on （a） micro-dimpled silicon surfaces, （b） bare silicon surfaces, and （c） octadecyltrichlorosilane （OTS） treated silicon surfaces at different relative humidity （RH） levels separately. It is found that above a critical RH level, the capillary pull-off force increases abruptly and that the micro-dimple textured surface has a lower critical RH value as well as a higher pull-off force value than the other two surfaces. A micro topography parameter, namely sidewall area ratio, is found to play a major role in controlling the capillary pull-off force. Furthermore, micro-dimpled silicon surface is also proved to be not sensitive to variation in RH level, and can realize a stable and decreased friction coefficient compared with un-textured silicon surfaces. The reservoir-like function of micro dimples is considered to weaken or avoid the breakage effect of liquid bridges at different RH levels, thereby maintaining a stable frictional behaviour.

关键词： micro-dimpled surface capillary pull-off force frictional property relative humidity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analysis of the human Atox 1 homologue in Wilson patients

引用

World Journal of Gastroenterology 2008年第15期14卷 2383-2387页

作者： Isabel Simon Mark schaefer Jürgen Reichert Wolfgang Stremmel Department of Gastroenterology University of HeidelbergGermany

AIM: To analyze the metallochaperone antioxidant-1 (Atox1) gene sequence in Wilson disease patients. METHODS: Mutation analysis of the four exons of the Atox1 gene including the intron- exon boundaries was performed in 63 Wilson disease patients by direct sequencing. RESULTS: From 63 selected patients no mutations were identified after the entire coding region including the intron- exon boundaries of Atox1 were sequenced. One known polymorphism within the Atox1 gene (5’UTR -99 T>C) in 31 (49%) of the Wilson patients as well as one previously undescribed variation (5’UTR -68 C>T) in 2 of the Wilson patients could be detected. Statistical analyses revealed that the existence of a variation within the Atox1- gene showed a tendency towards an earlier onset of the disease. CONCLUSION: Based on the data of this study, no major role can be attributed to Atox1 in the pathophysiology or clinical variation of Wilson disease.

关键词： Antioxidant-1 Wilson disease Wilson's disease protein Mutation analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

如何理解有意义的工作环境

引用

中国质量 2024年第5期 69-70页

作者：克里斯汀·舍弗尔王晓生

贵组织是否正面临员工的高流动率和低签约率的挑战，特别是一线员工？更重要的是你们是否准备好面对劳动者的需求，以在将来可以吸引和保持高业绩员工？无论哪种情况，罗伯特马钦科都能给大家带来新想法、新启发。马钦科是踪迹之径（Tra...

贵组织是否正面临员工的高流动率和低签约率的挑战，特别是一线员工？更重要的是你们是否准备好面对劳动者的需求，以在将来可以吸引和保持高业绩员工？无论哪种情况，罗伯特马钦科都能给大家带来新想法、新启发。马钦科是踪迹之径（TrailPaths）公司的总裁，该公司关注于建立有意义的员工环境，他将在美国第35届波多里奇追求卓越大会闭幕式上发表第二个主旨发言。

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Encapsulating segment-like antimony nanorod in hollow carbon tube as long-lifespan,high-rate anodes for rechargeable K-ion batteries

引用

Nano Research 2019年第5期12卷 1025-1031页

作者： Wen Luo Feng Li Weiran Zhang Kang Han Jean-Jacques Gaumet Hans-Eckhardt schaefer Liqiang Mai Department of Physics School of ScienceWuhan University of TechnologyWuhan 430070China Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of ChinaHefei 230000China Division of Materials Science and Engineering Boston UniversityMA 02215USA State Key Laboratory of Advanced Technology for Materials Synthesis and Processing International School of Materials Science and Technology Wuhan University of TechnologyWuhan 430070China Laboratoire de Chimie et Physique: Approche Multi-echelles des Milieux Complexes Institut Jean BarriolUniversite de LorraineMetz 57070France Institute for Functional Matter and Quantum Technologies Stuttgart UniversityPfaffenwaldring 57Stuttgart 70569Germany

K-ion battery (KIB) is a new-type energy storage device that possesses potential advantages of low-cost and abundant resource of *** develop advanced electrode materials for accommodating the large size and high activity of potassium ion is of great ***,a segment-like antimony (Sb) nanorod encapsulated in hollow carbon tube electrode material (Sb@HCT) was *** from the virtue of abundant nitrogen doping in carbon tube,one-dimensional and hollow structure advantages,Sb@HCT exhibits excellent potassium storage properties:in the case of potassium bis(fluorosulfonyl)imide (KFSI) electrolyte,Sb@HCT displays a reversible capacity of up to 453.4 mAh·g^-1 at a current density of 0.5 A·g^-1 and good rate performance (a capacity of 211.5 mAh·g^-1 could be achieved at an ultrahigh rate of 5 A·g^-1).Additionally,Sb@HCT demonstrates excellent long-cycle stability at a current density of 2 A·g^-1 over 120 ***,electrolyte optimization is an effective strategy for greatly improving electrochemical *** ex-situ characterizations,we disclosed the potassiation of Sb anode is quite reversible and undergoes multistep processes,combining solid solution reaction and two-phase reaction.

关键词： K-ion battery antimony anode hollow carbon tube encapsulation electrolyte optimizati on potassium storage mechanism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Weak correlation of flower color and nectar-tube depth in temperate grasslands

引用

Journal of Plant Ecology 2017年第2期10卷 397-405页

作者： Julia Binkenstein Martina Stang Julien P.Renoult H.Martin schaefer Chair of Nature Conservation and Landscape Ecology Faculty of Environment and Natural ResourcesUniversity of FreiburgTennenbacher Straße 479106 FreiburgGermany Section Plant Ecology and Phytochemistry Institute of Biology LeidenLeiden UniversitySylviusweg 722300 RA LeidenThe Netherlands Institute of Arts Creation Theory and Aesthetics UMR 8218-CNRS47 r.des bergers75015 ParisFrance Fundacion Jocotoco Lizardo GarcíaE9-104 y Andrés XauraP.O.Box 17-16-337QuitoEcuador

Aims Nectar is one of the most common floral rewards offered to pol-linators by *** on the plant species,nectar is offered openly or in tubes of various lengths restricting accessibility of this resource for flower visitors with short *** attract-ing pollinators that match floral morphology increases pollination efficiency,flowers could profit from signaling nectar-tube depth to *** flower colors are important signals in plant-pol-linator communication,we investigated whether and which differ-ent chromatic or achromatic aspects of flower color might indicate nectar-tube depth or whether flower colors facilitate the differen-tiation between flowers with long nectar tubes by means of high chromatic *** To this end,we collected flower reflectance spectra of 135 grassland plant *** analyzed flower colors as raw reflectance spectra in principal component analysis(PCA)and in the color space of *** Findings The correlation between flower colors and tube depths was *** the bee’s point of view,blue flowers had on average deeper tubes than green,blue-green and UV-green flowers potentially allow-ing insects to predict tube depths based on blue *** purity did not correlate with nectar-tube depth,nor did the chromatic uniqueness of flower colors in the honeybee color *** wavelength showed a significant but very weak correlation with tube *** achromatic green contrast decreased with increasing tube depth as did brightness;thus deep tubes were less conspicuous than shallow *** components resulting from PCA did not or only slightly correlate with tube *** results illustrate that flower colors may have a certain potential to indicate tube depth,*** accessibility,from a bee’s perspective.

关键词： vision pollination efficiency plant-animal interaction nectar tube depth

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：