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the kinesin-like proteins, KAC1/2, regulate actin dynamics underlying chloroplast light-avoidance in Physcomitrella patens

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Journal of Integrative Plant Biology 2015年第1期57卷 106-119页

作者： Zhiyuan Shen Yen-Chen Liu Jeffrey P.Bibeau Kyle P.Lemoi erkan tüzel Luis Vidali Department of Biology and Biotechnology Worcester Polytechnic Institute Department of Physics Worcester Polytechnic Institute

In plants, light determines chloroplast position; these organelles show avoidance and accumulation re- sponses in high and low fluence-rate light, respectively. Chloroplast motility in response to light is driven by cytoskeletal elements. the actin cytoskeleton mediates chloroplast photorelocation responses in Arabidopsis thali- ana. In contrast, in the moss Physcomitrella patens, both, actin filaments and microtubules can transport chloroplasts. Because of the surprising evidence that two kinesin-like proteins （called KACs） are important for actin-dependent chloroplast photorelocation in vascular plants, we wanted to determine the cytoskeletal system responsible for the function of these proteins in moss. We performed gene- specific silencing using RNA interference in P. patens. We confirmed existing reports using gene knockouts, that PpKAC1 and PpKAC2 are required for chloroplast dispersion under uniform white light conditions, and that the two proteins are functionally equivalent. to address the specificcytoskeletal elements responsible for motility, this loss-of- function approach was combined with cytoskeleton-targeted drug studies. We found that, in P. patens, these KACs mediate the chloroplast light-avoidance response in an actin filament- dependent, rather than a microtubule-dependent manner. Using correlation-decay analysis of cytoskeletal dynamics, we found that PpKAC stabilizes cortical actin filaments, but has no effect on microtubule dynamics.

关键词： Actin filaments cytoskeleton intracellular transport kinesin-14 light-avoidance response microtubule moss RNAi

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the Motor Kinesin 4II Is Important for Growth and Chloroplast Light Avoidance in the Moss <i>Physcomitrella patens</i>

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American Journal of Plant Sciences 2017年第4期8卷 791-809页

作者： Daniel MacVeigh-Fierro erkan tüzel Luis Vidali Department of Biology and Biotechnology Worcester Polytechnic Institute Worcester MA USA Department of Physics Worcester Polytechnic Institute Worcester MA USA

Understanding how plant cells adapt dynamically to changes in the environment is a fundamental problem of plant biology. Under many conditions, plant cells respond to environmental changes by modifying their intracellular organization. A critical example of intracellular reorganization is chloroplast photo-relocation, which is required for optimal energy harvesting and avoiding photodamage. A key system responsible for the spatial organization of intracellular components is the microtubule cytoskeleton and its associated motor proteins, kinesins. Here we tested the hypothesis that members of the kinesin 4II subfamily are important for chloroplast photo-relocation in the moss Physcomitrella patens. Most land plants, including P. patens, use an actin cytoskeleton-dependent mechanism to transport chloroplasts in response to light. In addition to the actin-based system, P. patens can also transport chloroplasts via a microtubule-dependent mechanism, which is absent in flowering plants. Here, we used a P. patens line that contains an inducible RNAi system to silence all three kinesin 4-II genes present in this moss and evaluated their participation in the microtubule-dependent chloroplast light avoidance response. Because we found a significant effect on cell growth when kinesin 4IIs are silenced, we took advantage of the inducible system to establish a reproducible and quantitative assay to evaluate chloroplast photo-relocation in full-grown cells. Using a laser scanning confocal-based chloroplast light avoidance response assay, we found a reduction in chloroplast motility when kinesin 4IIs were silenced. Hence, in addition to identifying a role for kinesin 4II proteins in protonemal cell growth, our results strongly support the hypothesis that these kinesins play an important role in the chloroplast light avoidance response.

关键词： Microtubules Cytoskeleton Chloroplast Motility Polarization Photo-Relocation

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Message from Editors

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CES transactions on Electrical Machines and Systems 2024年第3期8卷 241-243页

作者： tayfun GUNDOGDU Udochukwu B.AKURU Zhongze WU Guven KOMURGOZ erkan MESE Okan OZGONENEL Ozan KEYSAN Mehmet Onur GULBAHCE Department of Electrical Engineering Tshwane University of Technology South Africa Southeast University Istanbul technical University Ege University Ondokuz Mayis University Middle East technical University

AS the world embarks on a path toward electrification and strives to achieve net-zero greenhouse gas emissions,the role of electrical machines becomes increasingly vital across various *** urgent need for decarbonization and improved energy efficiency has directed attention to rare-earth-free electrical machines as a promising *** technologies offer a sustainable alternative to permanent magnet-based machines,which rely heavily on rare earth materials like neodymium and *** extraction and processing of these materials result in hazardous by-products that cause significant ecological harm,particularly near mining sites and refineries.

关键词： solution rare earth

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差异与耦合:电影表演与戏剧表演的融合发展

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电影评介 2024年第5期 70-74页

作者：谈尔侃上海视觉艺术学院表演艺术学院

电影理论家乔托·卡努杜将电影视为“第七艺术”,即静态艺术和动态艺术、时间艺术和空间艺术、造型艺术和节奏艺术的结合体。而这一宣言也将最初的电影引入了艺术殿堂。电影作为其他艺术的综合形式,首先在展现自身的过程中大量地借鉴了... 详细信息

电影理论家乔托·卡努杜将电影视为“第七艺术”,即静态艺术和动态艺术、时间艺术和空间艺术、造型艺术和节奏艺术的结合体。而这一宣言也将最初的电影引入了艺术殿堂。电影作为其他艺术的综合形式,首先在展现自身的过程中大量地借鉴了戏剧表演程式,以演员的表演作为轴线使故事得以展开;其次,电影自诞生以来虽然仅有百年历史,但其作为一门年轻的艺术形式却经历了三次重大的技术革命,其每一次的技术变革都为电影表演带来新的机遇与挑战。纵观电影的发展历程,电影艺术一直在革新与发展。电影表演与戏剧表演之间也相互影响、逐步深入。本文从表演形式的差异化入手对电影表演进行更为深入的分析,有助于更好地把握电影表演艺术的迭代历程。

关键词：电影表演戏剧表演电影理论家静态艺术乔托艺术殿堂差异化节奏艺术

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Enhancing the efficiency of cabin heaters in emergency shelters after earthquakes through an optimized fuzzy controller

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建筑模拟(英文) 2023年第9期16卷 1759-1776页

作者： erkan Duman Dila Seckin Computer Engineering Department Engineering FacultyFirat UniversityElazig 23119Turkey

In just one and half minutes,more than fifty thousand died due to the 7.7 and 7.6 magnitude earthquakes that struck turkey’s southeast on February 6,2023;thousands of families who barely escaped struggled to survive in the freezing weather.A warm shelter was the most basic requirement of these *** buildings are a rapid and easy solution to this ***,there is a need for a more effective and safe heating option than a wood fire for these *** this study,cabin heaters,which allow truck drivers to warm up when they park their vehicles to sleep,are specially optimized for emergency shelters after an *** optimized fuzzy controller was developed to use in such buildings,which allows an air–fuel ratio in the combustion chamber of the cabin heater to be controlled adaptively based on system dynamics to get lower carbon emissions and fuel *** tRNSYS software was used to establish the transient simulation model of a cabin heater with a capacity of 4 kW for a typical 21 m^(2) shelter building in turkey’s cold *** developed fuzzy controller carried out the heating process of this shelter from the 15th of November to the 15th of *** of using expert knowledge,the Gray Wolf Optimization(GWO)method was applied to optimize the fuzzy controller parameters developed for the cabin *** the optimized fuzzy controller,the fuel consumption at the end of the heating season was reduced by an average of 0.2 L/h,and the cabin heater’s efficiency increased by more than 13%.Our simulation results show that the intelligent controller we developed could improve diesel fuel combustion efficiency by up to 85%.

关键词： parking heater cabin heater emergency shelters fuzzy controller gray wolf optimization tRNSYS simulation

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Palliation of malignant esophageal obstruction and fistulas with self expandable metallic stents

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World Journal of Gastroenterology 2010年第45期16卷 5739-5745页

作者： Ahmet Dobrucali erkan Caglar Division of Gastroenterology Department of Internal MedicineCerrahpasa Medical FacultyIstanbul UniversityIstanbul 34300Turkey

AIM: to evaluate the efficacy of self expandable metallic stents (SEMS) in patients with malignant esophageal obstruction and fistulas. MEtHODS: SEMS were implanted in the presence of fluoroscopic guidance in patients suffering from advanced and non-resectable esophageal, cardiac and invasive lung cancer between 2002 and 2009. All procedures were performed under conscious sedation. All patients had esophagus obstruction and/or fistula. In all patients who required reintervention, recurrence of dysphagia, hemorrhage, and fistula formation were indications for further endoscopy. Patients' files were scanned retrospectively and the obtained data were analyzed using SPSS 13.0 for Windows. the χ 2 test was used for categorical data and was analysis of variance for noncategorical data. Patients' long-term survival was assessed using the Kaplan-Meier method. RESULtS: Stents were successfully implanted in 90 patients using fluoroscopic guidance. Reasons for stent implantation in these patients were esophageal stricture (77/90, 85.5%), external pressure (8/90, 8.8%) and tracheo-esophageal fistula (5/90, 5.5%). Dysphagia scores (mean ± SD) were 3.37 ± 0.52 before and 0.90 ± 0.43 after stent implantation (P = 0.002). Intermittent, nonmassive hemorrhage due to the erosion caused by the distal end of the stent in the stomach occurred in only one patient who received implementation at cardioesophageal junction. Mean survival following stenting was 134.14 d (95% confidence interval: 94.06-174.21).CONCLUSION: SEMS placement is safe and effective in the palliation of dysphagia in selected patients with malignant esophageal strictures.

关键词： Esophagus cancer Stenosis Stents Complication Dysphagia

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Photonic crystal structures:Beam deflector and beam router

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Chinese Physics B 2018年第4期27卷 320-326页

作者： Utku Erdiven erkan tetik Faruk Karadag Department of Physics Arts and SciencesCukurova University Computer Education and Instructional technology Department of EducationUsak University

We investigate the optical characteristic,transverse magnetic（tM） and transverse electric（tE） band of twodimensional（2 D） square lattice photonic crystal structure,which is composed of cylindrical air regions positioned at the corners of the square shaped dielectric *** obtain the wide photonic bandwidths between tM1–tM2 and tM3–tM4 *** to the results,we demonstrate the band gaps close to each other in the tM and tE frequencies for proposed *** resulting photonic gaps are formed to be about 8% at the higher frequencies of tE modes（tE4–tE5）and tM modes（tM7–tM8 and tM9–tM10）.In addition,we examine isotropically generated structures for light guiding properties and observe that the light is directed in a particular route without using any *** also investigate the self-collimation effect with the designed *** obtained results reveal the influences of the radius of cylindrical air holes and the angle between these air holes on absolute and partial photonic band ***,we observe the tM and tE band gaps that *** is thought that the obtained band overlap will provide an easy way to produce the photonic crystals in practical applications like photonic insensitive *** is also believed that these results can provide the photonic crystal structures to work as a beam deflecting and beam router in integrated optical circuit applications.

关键词： photonic bandgap materials electromagnetic wave propagation beam characteristics

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A New Metaheuristic Approach to Solving Benchmark Problems: Hybrid Salp Swarm Jaya Algorithm

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Computers, Materials & Continua 2022年第5期71卷 2923-2941页

作者： erkan Erdemir Adem Alpaslan Altun Department of Information technologies Tokat Vocational and Technical Anatolian High SchoolMerkez/Tokat60030Turkey Department of Computer Engineering Faculty of TechnologyKonya Selcuk UniversitySelcuklu/Konya42130Turkey

Metaheuristic algorithms are one of the methods used to solve optimization problems and find global or close to optimal solutions at a reasonable computational *** with other types of algorithms,in metaheuristic algorithms,one of the methods used to improve performance and achieve results closer to the target result is the hybridization of *** this study,a hybrid algorithm(HSSJAYA)consisting of salp swarm algorithm(SSA)and jaya algorithm(JAYA)is *** speed of achieving the global optimum of SSA,its simplicity,easy hybridization and JAYA’s success in achieving the best solution have given us the idea of creating a powerful hybrid algorithm from these two *** hybrid algorithm is based on SSA’s leader and follower salp system and JAYA’s best and worst solution *** works according to the best and worst food source *** this way,it is thought that the leader-follower salps will find the best solution to reach the food *** hybrid algorithm has been tested in 14 unimodal and 21 multimodal benchmark *** results were compared with SSA,JAYA,cuckoo search algorithm(CS),firefly algorithm(FFA)and genetic algorithm(GA).As a result,a hybrid algorithm that provided results closer to the desired fitness value in benchmark functions was *** addition,these results were statistically compared using wilcoxon rank sum test with other *** to the statistical results obtained from the results of the benchmark functions,it was determined that HSSJAYA creates a statistically significant difference in most of the problems compared to other algorithms.

关键词： Metaheuristic optimization benchmark algorithm swarm hybrid

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Illumination of parameter contributions on uneven break： phenomenon in underground stoping mines

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International Journal of Mining Science and technology 2016年第6期26卷 1095-1100页

作者： Jang Hyongdoo topal erkan Kawamura Youhei Department of Mining Engineering and Metallurgical Engineering Western Australian School of Mines Curtin University Perth 6102 Australia Division of Intelligent Interaction technologies Faculty of Engineering Information and Systems University of Tsukuba Tsukuba 3058555 Japan

One of the most serious conundrum facing the stope production in underground metalliferous mining is uneven break （UB： unplanned dilution and ore-loss）. Although the UB has a huge economic fallout to the entire mining process, it is practically unavoidable due to the complex causing mechanism. In this study, the contribution of ten major UB causative parameters ha,; been scrutinised based on a published UB predicting artificial neuron network （ANN） model to put UB under the engineering management. two typical ANN sensitivity analysis methods, i.e., connection weight algorithm （CWA） and profile method （PM） have been applied. As a result of CWA and PM applications, adjusted Qrate （AQ） revealed as the most influential parameter to UB with contribution of 22,40% in CWA and 20,48% in PM respectively. the findings of this study can be used as an important reference in stope design, production, and reconciliation stages on underground stoping mine.

关键词： Unplanned dilution Ore-loss Underground metalliferous mining Uneven break Artificial neuron network

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A State-Based Peridynamic Formulation for Functionally Graded Euler-Bernoulli Beams

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Computer Modeling in Engineering & Sciences 2020年第8期124卷 527-544页

作者： Zhenghao Yang erkan Oterkus Selda Oterkus PeriDynamics Research Centre Department of Naval ArchitectureOcean and Marine EngineeringUniversity of StrathclydeGlasgowG40LZUK

In this study,a new state-based peridynamic formulation is developed for functionally graded Euler-Bernoulli *** equation of motion is developed by using Lagrange’s equation and taylor *** axial and transverse displacements are taken into account as degrees of *** different boundary conditions are considered including pinned support-roller support,pinned support-pinned support,clamped-clamped and *** results are compared against finite element analysis results for transverse and axial deformations and a very good agreement is observed for all different types of boundary conditions.

关键词： Peridynamics Euler-Bernoulli beam functionally graded state-based

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