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Micropropagation of Eucalyptus grandis×*** AEC 224 clone

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Journal of Forestry Research 2017年第1期28卷 29-39页

作者： Cassiana de Oliveira Juliana Degenhardt-Goldbach Gisela Manuela de Franca Bettencourt erika Amano Luziane Franciscon Marguerite Quoirin Department of Plant Science Federal University of Parana1540Rua dos FuneionariosCuritibaParanaBrazil Embrapa-Forest 111Estrada da RibeiraColomboParanaBrazil Department of Technology Federal University of Parana210avenida CelFrancisco Heraiclito dos SantosCuritibaParanaBrazil Department of Botany Federal University of Parana210avenida CelFrancisco Heraiclito dos SantosCuritibaParanaBrazil

Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis x E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 μM BA and 0.5 μM NAA for another 30 days. In these media, the explant oxidation rate was high （95 %）. Thus, in order to reduce oxidation, different ;culture media were compared： WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 μM TDZ and 0.1μM NAA during 30 days for callus induction and then with 5.0 μM BA and 0.5 μM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA （2.46, 4.90 and 7.35μM）. The highest rooting percentage （35 %） was obtained on medium containing 2.46μM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %.

关键词： Callogenesis Eucalyptus urograndis Organogenesis WPM culture medium

紫坪铺水库造成孔隙弹性耦合变化及其对2008年汶川地震触发作用

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地球物理学报 2014年第10期57卷 3318-3331页

作者：陶玮 Timothy Masterlark 沈正康 erika Ronchin 张永中国地震局地质研究所地震动力学国家重点实验室北京100029 Department of Geology and Geological Engineering South Dakota School of Mines & Technology 北京大学地球与空间科学学院地球物理系北京100871 Department of Earth and Space Sciences University of CaliforniaLos Angeles Institute of Earth Sciences Jaume Almera Spanish National Research Council

位于川西地区龙门山断裂带附近的紫坪铺水库于2005年9月开始蓄水.约2.7年后,2008年5月12日,Mw7.9级汶川地震在龙门山断裂带上发生,两事件在时间和空间上的接近,揭示其可能相互关联,但前人的诸多研究给出了不同甚至是相反的结果.本研究... 详细信息

位于川西地区龙门山断裂带附近的紫坪铺水库于2005年9月开始蓄水.约2.7年后,2008年5月12日,Mw7.9级汶川地震在龙门山断裂带上发生,两事件在时间和空间上的接近,揭示其可能相互关联,但前人的诸多研究给出了不同甚至是相反的结果.本研究基于完全耦合孔隙弹性理论,利用二维有限元模型（FEM）,模拟水库蓄水造成的区域孔隙压力场和应力场的演化过程,基于库仑应力演化探讨其对龙门山断裂带活动的影响.模拟结果显示紫坪铺水库蓄水打破了原来的区域孔隙压力平衡,形成孔隙压力梯度源,向周围地壳传播;进而造成龙门山断裂带上库仑应力正值范围不断扩大,由浅入深影响到整条断层,尤其对浅层范围的加载作用明显,达上百千帕,为整个断层面的失稳提供了基础.震源区域库仑应力呈持续增长趋势,汶川地震发震前,增长了约数千帕-数十千帕,即使初期库仑应力为负,在未来某时刻库仑应力仍可能由负转正,并不断增大.通过计算汶川地震震源及其附近区域内相对于多种可能断层倾角的库仑应力,发现库仑应力随断层倾角增大而增加.因此整体来看,紫坪铺水库蓄水对龙门山断裂带起加载作用,有可能触发地震.对紫坪铺库区周围的小震分析也显示,蓄水以来小震明显增多、且不同时段小震发生密集区与水库距离逐渐增大,与孔隙压力扩散趋势一致.以上结果表明紫坪铺水库的蓄水增加了汶川地震的危险性.

关键词：紫坪铺水库完全孔隙弹性耦合应力触发汶川地震有限元

Geopoetical Analysis of Livonia During The Great Northern War Based on The Last Novik A Historical Novel by Ivan Lazhechnikov

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Journal of Literature and Art Studies 2018年第3期8卷 378-383页

作者： erika Kuzmina Warsaw University Warsaw Poland

Neonatal Fc receptor-targeted lignin-encapsulated porous silicon nanoparticles for enhanced cellular interactions and insulin permeation across the intestinal epithelium

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Bioactive Materials 2022年第3期7卷 299-315页

作者： Joao P.Martins Patrícia Figueiredo Shiqi Wang erika Espo Elena Celi Beatriz Martins Marianna Kemell Karina Moslova Ermei Makil Jarno Salonen Mauri A.Kostiainen Christian Celia Vincenzo Cerullo Tapani Viitala Bruno Sarmento Jouni Hirvonen Helder A.Santos Drug Research Program Division of Pharmaceutical Chemistry and TechnologyFaculty of PharmacyUniversity of HelsinkiFI-00014HelsinkiFinland Department of Pharmacy University of Chieti-Pescara“G d’Annunzio”I-66100ChietiItaly Drug Research Program Division of Pharmaceutical BiosciencesFaculty of PharmacyUniversity of HelsinkiFI-00014HelsinkiFinland Department of Chemistry University of HelsinkiFI-00014HelsinkiFinland Department of Physics and Astronomy University of TurkuFI-20014TurkuFinland Biohybrid Materials Department of Bioproducts and BiosystemsAalto UniversityFI-00076AaltoFinland i3S-Instituto de Investigaçao e Inovaçao em Saúde University of Porto4200-135PortoPortugal INEB-Instituto de Engenharia Biomedica University of Porto4200-135PortoPortugal CESPU-Instituto de Investigaçao e Formaçao Avançada em Ciencias e Tecnologias da Saúde 4585-116GandraPortugal Helsinki Institute of Life Science(HiLIFE) University of HelsinkiFI-00014HelsinkiFinland

Oral insulin delivery could change the life of millions of diabetic patients as an effective,safe,easy-to-use,and affordable alternative to insulin injections,known by an inherently thwarted patient ***,we designed a multistage nanoparticle(NP)system capable of circumventing the biological barriers that lead to poor drug absorption and bioavailability after oral *** nanosystem consists of an insulin-loaded porous silicon NP encapsulated into a pH-responsive lignin matrix,and surface-functionalized with the Fc fragment of immunoglobulin G,which acts as a targeting ligand for the neonatal Fc receptor(FcRn).The developed NPs presented small size(211±1 nm)and narrow size *** NPs remained intact in stomach and intestinal pH conditions,releasing the drug exclusively at pH 7.4,which mimics blood *** formulation showed to be highly cytocompatible,and surface plasmon resonance studies demonstrated that FcRn-targeted NPs present higher capacity to interact and being internalized by the Caco-2 cells,which express FcRn,as demonstrated by Western ***,in vitro permeability studies showed that Fc-functionalized NPs induced an increase in the amount of insulin that permeated across a Caco-2/HT29-MTX co-culture model,showing apparent permeability coefficients(Papp)of 2.37×10􀀀6 cm/s,over the 1.66×10􀀀6 cm/s observed for their non-functionalized ***,these results demonstrate the potential of these NPs for oral delivery of anti-diabetic drugs.

关键词： Nanoparticles Porous silicon Lignin FcRn Insulin Oral drug delivery

多重劣化机制作用下混凝土的性能和耐久性（英文）

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硅酸盐学报 2015年第10期43卷 1420-1429页

作者： erika HOLT Miguel FERREIRA Hannele KUOSA Markku LEIVO Infrastructure Health VTT Technical Research Centre of Finland Ltd

基于近年来芬兰VT技术研究中心在混凝土性能和耐久性方面的研究成果,对多重劣化机制共同作用下混凝土耐久性能表现进行了综述。这些研究是建立在30余年来有关混凝土耐久性的研究结果之上,包括15年的现场暴露实验数据,有关混凝土耐久性... 详细信息

基于近年来芬兰VT技术研究中心在混凝土性能和耐久性方面的研究成果,对多重劣化机制共同作用下混凝土耐久性能表现进行了综述。这些研究是建立在30余年来有关混凝土耐久性的研究结果之上,包括15年的现场暴露实验数据,有关混凝土耐久性能是通过实验室加速实验结果和现场暴露实验结果共同评价的。结果显示,实验室的单一因素作用下的混凝土耐久性加速劣化结果与多因素共同作用下的暴露实验和工程现场测试结果有很大的差异。现场测试能够获得较长期的混凝土耐久性结果,这可以用来校准和验证混凝土耐久性和寿命预测模型,并引导继续对多因素共同作用下混凝土的耐久性能进行更全面深入的研究。

关键词：混凝土耐久性多重因素作用现场暴露实验服役年限模拟

Short-term microbial responses to soluble inorganic P input in a tropical lowland rain forest in Amazonia

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Pedosphere 2021年第6期31卷 964-972页

作者： Yuri W.L.DE SOUSA erika BUSCARDO Carlos A.N.QUESADA Henrique E.M.NASCIMENTO Laszlo NAGY National Research Institute for Amazonia Manaus 69067-375(Brazil) Department of Forestry University of BrasiliaBrasilia 70910-900(Brazil) Centre for Functional Ecology University of CoimbraCoimbra 3000-456(Portugal) Department of Animal Biology University of CampinasCampinas 13083-862(Brazil)

In non-flooded lowland rain forests with low soil phosphorus(P)in parts of Amazonia,P cycling largely occurs via leaf litter recycling by arbuscular mycorrhizal(AM)fungal *** high input of P into these ecosystems occurs during drought years with increased *** the length and frequency of drought events are projected to increase in the region,a single-dose nutrient addition experiment was carried out to test how this would impact P *** application rate of 4 kg P ha^(-1) was used,which corresponds to twice the amount of litter-derived P in an average *** was hypothesized that i)the added mineral P would be immobilized by soil microorganisms,leading to measurable increase in soil microbial biomass carbon(C)and P and ii)AM colonization rate would be reduced by the pulse in mineral P available for plant *** results did not support either of our *** addition of P did not have an effect on AM root colonization,nor was P immobilized by soil microbiota during the experimental *** lack of a difference between the control and treatment at our study site could be attributed to the relatively low one-off dose of P applied that did not change either the colonization rate of roots by AM fungi or the amount of soil available labile *** obtain a mechanistic understanding of the availability,capture,and use of P by plant-symbiont associations in tropical rain forest ecosystems,further integrated studies of the soil-plant system combining long-term nutrient manipulations,modeling,and experimental approaches are required.

关键词： Amazon basin arbuscular mycorrhizal fungi nutrient addition phosphorus cycling soil microbial biomass

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导师越多越好

科学新闻 2022年第5期24卷 60-61页

作者： erika Moore 倪伟波佛罗里达大学

“不幸的是，我现在没有时间处理师徒关系。”“我不确定是否能帮上忙。”“我们怎么又见面了？”以上只是我在寻求更多导师过程中收到的一些回复。在研究生院学习的早期，这样的反应让我感到沮丧，与冒名顶替综合征斗争，并努力保持信...

“不幸的是，我现在没有时间处理师徒关系。”“我不确定是否能帮上忙。”“我们怎么又见面了？”以上只是我在寻求更多导师过程中收到的一些回复。在研究生院学习的早期，这样的反应让我感到沮丧，与冒名顶替综合征斗争，并努力保持信心再次回复或发送电子邮件。然而，从那以后，我明白了它们是构建导师关系网的内在组成部分——而且为此付出的痛苦是值得的。

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Label-free sensing of ultralow-weight molecules with all-dielectric metasurfaces supporting bound states in the continuum

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Photonics Research 2018年第7期6卷 726-733页

作者： SILVIA ROMANO GIANLUIGI ZITO STEFANIA TORINO GIUSEPPE CALAFIORE erika PENZO GIUSEPPE COPPOLA STEFANO CABRINI IVO RENDINA VITO MOCELLA Institute for Microelectronics and Microsystems Unit of Naples National Council of Research Institute of Protein Biochemistry National Council of Research Lawrence Berkeley National Laboratory Molecular Foundry Division

The realization of an efficient optical sensor based on a photonic crystal metasurface supporting bound states in the continuum is reported. Liquids with different refractive indices, ranging from 1.4000 to 1.4480, are infiltrated in a microfluidic chamber bonded to the sensing dielectric metasurface. A bulk liquid sensitivity of 178 nm/RIU is achieved, while a Q-factor of about 2000 gives a sensor figure of merit up to 445 in air at both visible and infrared excitations. Furthermore, the detection of ultralow-molecular-weight(186 Da) molecules is demonstrated with a record resonance shift of 6 nm per less than a 1 nm thick single molecular layer. The system exploits a normal-to-the-surface optical launching scheme, with excellent interrogation stability and demonstrates alignment-free performances, overcoming the limits of standard photonic crystals and plasmonic resonant configurations.

关键词：高效光学传感器折射率理论研究通讯技术

The CircRNAs for Diagnostic, Prognostic, and Therapy in Alzheimer’s Disease

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Advances in Bioscience and Biotechnology 2022年第12期13卷 507-526页

作者： erika Alejandra Cabrera-Reyes Miguel Ángel Jiménez-Acosta Mayte Lizeth Padilla-Cristerna Marco Antonio Meraz-Ríos Department of Molecular Biomedicine Center of Investigation and Advances Studies IPN México City México

The structure and biological function of circular RNAs (circRNAs) in regulating gene expression in a cell is far from known. CircRNAs are unique molecules that contain potent regulatory elements. CircRNAs actively interact with miRNAs (sponging), affecting their regulation and functions. In addition, circRNAs have roles in transcriptional regulation, splicing, and peptide synthesis. With all these properties, circRNAs could play an essential role in diseases, especially Alzheimer’s. Their role in early diagnosis, previous to present symptoms, prognosis associated with neuropathological AD of specific circRNAs, and one of their primary functions is to act as a sponge for miRNAs, which could be a starting point for future gene therapy. This review aims to summarize the current knowledge of these exciting molecules and their potential use as new markers for AD risk. This article will focus on circRNAs deregulated in Alzheimer’s.

关键词： CircRNA Alzheimer’s Disease miRNAs Diagnosis Therapy Dementia