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小胶质细胞填充OHSC:研究体内成熟小胶质细胞的培养体系

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神经损伤与功能重建 2016年第5期11卷 F0003-F0003页

作者： Annette Masuch Rianne van der Pijl Lisa Füner Yochai Wolf Bart Eggen erik Boddeke Knut Biber 唐颖馨

最近研究显示,分枝样小胶质细胞在大脑中发挥各种作用。但是,原代培养小胶质细胞这一研究模型并不足以研究这种独特的细胞。本研究提供了一种新的研究方法:培养海马脑片(organotypic hippocampal slice cultures,OHSC),从成年鼠脑中分... 详细信息

最近研究显示,分枝样小胶质细胞在大脑中发挥各种作用。但是,原代培养小胶质细胞这一研究模型并不足以研究这种独特的细胞。本研究提供了一种新的研究方法:培养海马脑片(organotypic hippocampal slice cultures,OHSC),从成年鼠脑中分离分枝样小胶质细胞,并将该细胞移植到海马脑片中(去掉相应位置的细胞),得到小胶质细胞移植海马脑片(microglia-replenished OHSC,Mrep-OHSC)。移植后的小胶质细胞和海马组织融合,分枝延展,与鼠脑中的原有的分枝样小胶质细胞无法区分。从TNFα敲除小鼠中提取小胶质细胞,并移植到野生型小鼠脑片,与来自于特异性小胶质细胞TNFα敲除小鼠(CX3CR1cre/TNFαfl/fl)的脑片具有相似的特性。本研究还显示,移植到海马脑片中的小胶质细胞保持了其原有的(在体)功能和特性。从早老模型小鼠(ERCC1△/ko)中提取小胶质细胞并移植到野生型海马脑片中,这些小胶质细胞维持其原有表型并继续高表达Mac2。综上所述,本研究证明Mrep-OHSC是构建嵌合子脑片的独特的组织培养模型,可用于研究在体不同表型的小胶质细胞的功能。

关键词： ERCC1 NMDA TNFα 海马神经保护填充物

肿瘤恶性转化与C/EBPβ和STAT3基因相关(英文)

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中华神经外科疾病研究杂志 2010年第1期9卷 22-22页

作者： Maria Stella Carro Wei Keat Lim Mariano javier Alvarez Robert j. Bollo Evan Y. Snyder erik P. Sulman Sandrine L. Anne Fiona Doetsch Howard Colman Anna Lasorella Ken Aldape Andrea Califano Antonio Iavarone

The inference of transcriptional networks that regulate transitions into physiological or pathological cellular states remains a central challenge in systems biology. A mesenchymal phenotype is the hallmark of tumour aggressiveness in human malignant glioma,but the regulatory programs responsible for implementing the associated molecular signature are largely unknown. Here we show that reverse-engineering and an unbiased interrogation of a glioma-specific regulatory network reveal the transcriptional module that activates expression of mesenchymal genes in malignant glioma. Two transcription factors (C/EBPβ and STAT3) emerge as synergistic initiators and master regulators of mesenchymal transformation. Ectopic co-expression of C/EBPβ and STAT3 reprograms neural stem cells along the aberrant mesenchymal lineage,whereas elimination of the two factors in glioma cells leads to collapse of the mesenchymal signature and reduces tumour aggressiveness. In human glioma,expression of C/EBPβ and STAT3 correlates with mesenchymal differentiation and predicts poor clinical outcome. These results show that the activation of a small regulatory module is necessary and sufficient to initiate and maintain an aberrant phenotypic state in cancer cells.

关键词：脑瘤细胞分子生理学临床分析

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悼念erik Moisar教授

影像科学与光化学 1991年第2期 160-160页

作者： j.Lohmann F.Granzer Agfa公司研究部 Frankfurt大学应用物理系主任教授

erik Moisar教授不幸于1990年12月20日猝然去世。他将自己的毕生精力献给了感光科学事业。 1945年Moisar结束了他在柏林的学习,在Stoess明胶广开始了他的职业生涯。1959年他到Agfa,1972年以后他作为名誉教授一直在Frankfurt大学任教。1... 详细信息

erik Moisar教授不幸于1990年12月20日猝然去世。他将自己的毕生精力献给了感光科学事业。 1945年Moisar结束了他在柏林的学习,在Stoess明胶广开始了他的职业生涯。1959年他到Agfa,1972年以后他作为名誉教授一直在Frankfurt大学任教。1987年从Agfa退休后全身心地投入了在Frankfurt的教学和研究工作。从一开始,Moisar就致力于照相过程原理的研究。作为一个有30年经验的研究人

关键词：卤化银晶体 erik Moisar 影像科学

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相互尊重文明对话

国际人才交流 2024年第2期 I0001-I0001页

作者：埃里克·古德布兰德·索尔海姆 “一带一路”绿色发展国际联盟咨询委员会

在公元13世纪,一位欧洲的年轻人马可·波罗(Marco Polo)从家乡意大利威尼斯一路旅行来到了中国,他在中国旅行时间长达20年,所到之处都令其印象深刻。回到欧洲后,他被人戏称为“百万二世”,因为人们认为他把自己的经历夸大了一百万倍。... 详细信息

在公元13世纪,一位欧洲的年轻人马可·波罗(Marco Polo)从家乡意大利威尼斯一路旅行来到了中国,他在中国旅行时间长达20年,所到之处都令其印象深刻。回到欧洲后,他被人戏称为“百万二世”,因为人们认为他把自己的经历夸大了一百万倍。欧洲人很难相信,宋朝的两个都城即开封和临安的人口,都达到了100万到200万之间,比欧洲人口最多的城市巴黎还要多15倍。关于中国辉煌的故事让人震惊,令人难以置信。

关键词：文明对话意大利威尼斯马可·波罗旅行时间相互尊重印象深刻 13世纪

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瑞士巴塞尔老工业混凝土仓房改建

城市环境设计 2019年第2期118卷 184-197页

作者： Agnete jukneviciute Alexandra Gustaffson Andreas johansen Annette jensen Barbara Srpkova Buster Christensen Camila Stadler Dennis Rasmussen Dominic Black Enea Michelesio erik de Haan Gül Ertekin Franck Fdida Helen Chen Ioannis Gio jan Magasanik jesper Andersen Lorenzo Boddi Marcelina Kolasinska Teresa Fernandez Martin Voelkle Miao Zhang Michael Sch?nemann Mikkel Marcker Stubgaard Ole Elkj?r-Larsen Ricardo Palma Ryohei Koike Sergiu Calacean Tobias Hjortdal Laurian Ghinitoiu 朱翰林

巴塞尔的Transitlager坐落于Dreispitz的未来城区中心位置,是众多用于美术馆、餐厅和创意产业的新老建筑中的其中一座。它位于设想的Soho区和皇后区的边缘。与城市一样,周围的工业区域也充斥着基础设施的几何形体。通过对原仓库进行新... 详细信息

巴塞尔的Transitlager坐落于Dreispitz的未来城区中心位置,是众多用于美术馆、餐厅和创意产业的新老建筑中的其中一座。它位于设想的Soho区和皇后区的边缘。与城市一样,周围的工业区域也充斥着基础设施的几何形体。通过对原仓库进行新的功能规划和扩建,使其成为一座多功能的建筑,我们设计将艺术、商业、工

关键词：巴塞尔核心筒 UBS

An international survey of classification and treatment choices for group D retinoblastoma

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International journal of Ophthalmology(English edition) 2017年第6期10卷 961-967页

作者： Christina Scelfo jasmine H Francis Vikas Khetan Thomas jenkins Brian Marr David H Abramson Carol L Shields jacob Pe’er Francis Munier jesse Berry j.William Harbour Andrey Yarovoy Evandro Lucena Timothy G Murray Pooja Bhagia Evelyn Paysse Samuray Tuncer Guillermo L Chantada Annette C Moll Tatiana Ushakova David A Plager Islamov Ziyovuddin Carlos A Leal Miguel A Materin Xun-Da ji jose W Cursino Rodrigo Polania Hayyam Kiratli Charlotta All-Ericsson Rejin Kebudi Santosh G Honavar Vicktoria Vishnevskia-Dai Sidnel Epelman Anthony B Daniels jeanie D Ling Fousseyni Traore Marco A Ramirez-Ortiz Ophthalmic Oncology Service Department of Surgery Memorial Sloan Kettering Cancer Center New York 10065 USA Department of Ophthalmology Weill-Cornell Medical Center New York 10065 USA Department of Vitreoretinal and Ocular Oncology Sankara Nathralaya Chennai 600066 India Ocular Ontology Service Wills Eye Hospital Thomas Jefferson University Pennsylvania 19107 USA Hadassah - Hebrew University Medical Center Jerusalem 91120 Israel jules-Gonin Eye Hospital Lausanne 1004 Switzerland USC Roski Eye Institute Children＇s Hospital of Los Angeles California 90033 USA Ocular Oncology Service Bascom Palmer Eye Institute University of Miami Miller School of Medicine Florida 33136 USA Ocular Oncology Department S.N. Fyodorov Eye Microsurgery Federal State Institution Moscow 127486 Russia Department of Ocular Oncology Instituto Nacional de Cancer Rio de Janeiro 1122 Brazil Murray Ocular Oncology and Retina Miami Children＇s Hospital Florida 33143 USA St. joseph＇s Children＇s Hospital New Jersey 07504 USA Department of Ophthalmology Baylor College of Medicine Texas 77030 USA Department of Ophthalmology Ocular Oncology Istanbul Medical Faculty Istanbul University Istanbul 34452 Turkey Oncology Department Hospital JP Garrahan Buenos Aires 1245 Argentina Department of Ophthalmology VU University Medical Center Amsterdam 1081 the Netherlands Department of Head and Neck Tumors Science Research Institute of Pediatric Oncology and Hematology N.N. Blokhin Russian Cancer Research Center Moscow 115478 Russia Pediatric Ophthalmology and Adult Strabismus Riley Hospital for Children Indiana University Medical Center Indiana 46202 USA National Oncology Center of Uzbekistan Tashkent 110508 Uzbekistan nstituto Nacional de Pediatria Coyoacan 04530 Mexico Department of Ophthalmology and Visual Science Smilow Cancer Hospital at Yale New Haven CT 06520 USA Xin Hua Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200092 China Department of Ophthalmology Santa Casa Mi

AIM： To determine which IIRC scheme was used by retinoblastoma centers worldwide and the percentage of D eyes treated primarily with enucleation versus globe salvaging therapies as well as to correlate trends in treatment choice to IIRC version used and geographic region. METHODS： An anonymized electronic survey was offered to 115 physicians at 39 retinoblastoma centers worldwide asking about IIRC classification schemes and treatment patterns used between 2008 and 2012. Participants were asked to record which version of the IIRC was used for classification, how many group D eyes were diagnosed, and how many eyes were treated with enucleation versus globe salvaging therapies. Averages of eyes per treatment modality were calculated and stratified by both IIRC version and geographic region. Statistical significance was determined by Chi-square, ANOVA and Kruskal-Wallis tests using Prism. RESULTS： The survey was completed by 29% of physicians invited to participate. Totally 1807 D eyes were diagnosed. Regarding IIRC system, 27% of centers used the Children＇s Hospital of Los Angeles （CHLA） version, 33% used the Children＇s Oncology Group （COG） version, 23% used the Philadelphia version, and 17% were unsure. The rate for primary enucleation varied between 0 and 100% and the mean was 29%. By IIRC version, primary enucleation rates were： Philadelphia, 8%; COG, 34%; and CHLA, 37%. By geographic region, primary enucleation rates were： Latin America, 57%; Asia, 40%; Europe, 36%; Africa, 10%, US, 8%; and Middle East, 8%. However, systemic chemoreduction was used more often than enucleation in all regions except Latin America with a mean of 57% per center （P〈0.0001）. CONCLUSION： Worldwide there is no consensus on which IIRC version is used, systemic chemoreduction was the most frequently used initial treatment during the study period followed by enucleation and primary treatment modality, especially enucleation, varied greatly with regards to IIRC version used and geographic r

关键词： retinoblastoma oncology retina enucleation chemotherapy intra-arterial chemotherapy ophthalmic arterychemosurgery cancer

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观看日食后发生光诱发性黄斑损害

世界核心医学期刊文摘(眼科学分册) 2006年第3期 60-60页

作者： Kllmark F.P Ygge j. 秦雪娇 St. erik's Eye Hospital Polhemsgatan 50 S-112 82 Stockholm SwedenDr.

Purpose: To study the injury to and possible recovery of the visual function and foveal morphology in patients with photoinduced foveal injury due to watching the solar eclipse of August 11th, 1999 in Stockholm, Sweden. Methods: Fifteen patients, all of whom viewed the solar eclipse, were followed for 1 year, during which their visual symptoms were recorded and visual acuity (VA) was tested, and ophthalmoscopy and scanning laser ophthalmoscopy were performed. Results: Photo-induced foveal injury gave rise to subjective visual disturbances, reducedVA andmorphological changes in the fovea. Central scotomas could still be seen in all patients 1 year after the foveal injury. Conclusion: Photo-induced foveal injury gave rise to subjective visual disturbances, reduced VA and morphological changes in the fovea. Scanning laser ophthalmoscopy offers the possibility of detailed examination of small retinal lesions, which can sometimes be difficult to localizewith ophthalmoscopy.

关键词：黄斑视网膜眼底镜日食交食