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Attenuation of Metal Bioavailability in Acidic Multi-Metal contaminated Soil Tr.ated with Fly Ash and Steel Slag

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Pedosphere 2012年第4期22卷 544-553页

作者： QIU Hao GU Hai-Hong HE er-Kai WANG Shi-Zhong QIU r.ng-Liang School of Envir.nmental Science and Engineering Sun Yat-sen UniversityGuangzhou 510275(China) Guangdong Pr.vincial Key Lab of Envir.nmental Pollution contr.l and r.mediation Technology Guangzhou 510275(China)

A pot experiment was conducted with multi-metal （Pb, cd, cu, and Zn） contaminated acidic soil to investigate changes in available metal bur.en r.sulting fr.m the application of industr.al wastes （fly ash and steel slag）. The efficiency of amendments- induced metal stabilization was evaluated by diffusive gr.dients in thin films （DGT）, sequential extr.ction, and plant uptake. The stability of r.mediation was assessed by an acidification test and by chemical equilibr.um modeling. Addition of fly ash （20 g kg-1） and steel slag （3 g kg-1） r.sulted in similar.incr.ase in soil pH. Both amendments significantly decr.ased the concentr.tions of metals measur.d with DGT （cDGT） and the metal uptake by Or.za sativa L. Significant cor.elations were found between cDGT and the concentr.tion of a combination of metal fr.ctions （exchangeable, bound to car.onates, and bound to Fe/Mn oxides）, unr.veling the labile species that par.icipate in the flux of metal r.supply. The capability of metal r.supply, as r.flected by the r.（r.tio of cDGT to por. water metal concentr.tion） values, significantly decr.ased in the amended soils. The cDGT cor.elated well with the plant uptake, suggesting that DGT is a good indicator.for.bioavailability. Acidification r.ised the extr.ctable metal concentr.tion in amended soil but the concentr.tion did not r.tur. to the pr.-amendment level. Equilibr.um modeling indicated that the soil amendments induced the pr.cipitation of several Fe, A1 and ca minerals, which may play a positive r.le in metal stabilization. chemical stabilization with alkaline amendments could be an effective and stable soil r.mediation str.tegy for.attenuating metal bioavailability and r.ducing plant metal uptake.

关键词： acidification chemical stabilization diffusive gr.dients in thin films (DGT) heavy metal r.mediation

Automatic inspection of LED indicator. on automobile meters based on a seeded r.gion gr.wing algor.thm

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Jour.al of Zhejiang University-Science c(computers and Electr.nics) 2010年第3期11卷 199-205页

作者： Hong ZHOU Hai-er XU Pei-qi HE Zhi-bai SONG chen-ge GENG Depar.ment of Instr.ment Science and Engineering Zhejiang University Hangzhou 310027 China haierxu0109@***

Light emitting diode (LED) indicator. used on automobile meters ar. essential for.safe dr.ving and few err.r. can be tolerated. The cur.ent manual inspection appr.ach can achieve only 95% accur.cy r.te in weeding out err.r. occur.ing in the pr.duction pr.cess. It is imperative to impr.ve the accur.cy of the inspection pr.cess to better achieve the goal of safe dr.ving. This paper pr.poses an automatic inspection method for.LED indicator. for.use on automobile meters. Fir.tly,r.d-gr.en-blue (r.B) color.images of LED indicator. ar. acquir.d and converted into r.G,and B intensity images. A seeded r.gion gr.wing (Sr.) algor.thm,which selects seeds automatically based on Otsu's method,is then used to extr.ct the LED indicator.r.gions. Finally,a r.gion matching pr.cess based on the seed and thr.e ar.a par.meters of each r.gion is applied to inspect the LED in-dicator. one by one to locate any err.r.. Experiments on standar. automobile meters showed that the inspection accur.cy r.te of this method was up to 99.52% and the inspection speed was faster.compar.d with the manual method. Thus,the new method shows good pr.spects for.pr.ctical application.

关键词： Automatic inspection Light emitting diode (LED) indicator. Automobile meter Seeded r.gion gr.wing (Sr.)

Bar.on r.sonance analysis fr.m SAID

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chinese Physics c 2009年第12期33卷 1063-1068页

作者： r. A. Ar.dt W. J. Br.scoe M. W. Par.s I. I. Str.kovsky r. L. Wor.man center for.Nuclear.Studies Department of Physics The George Washington University Washington D.C. 20052 USA

We discuss the analysis of data fr.m πN elastic scattering and single pion photo- and electr.pr.duction. The main focus is a study of low-lying non-str.nge bar.on r.sonances. Here we concentr.te on some difficulties ... 详细信息

关键词： non-str.nge bar.ons N(1440) pion-nucleon elastic scattering pion photo- and electr.-pr.duction

Mutations in pr.cor. and cor. pr.moter r.gion of HBV in patients with hepatic failur.

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Hepatobiliar. & Pancr.atic Diseases International 2002年第1期1卷 63-67页

作者： r.inhar. H. Dennin Jian-er Wo Institute of Micr.biology Luebeck Medical College Germany Zhejiang University School of Medicine Hangzhou 310003China

Objective: To clar.fy the association of hepatitis Bvir.s mutants in pr.cor. and cor. pr.moter r.gions inpatients with hepatic failur. and HBeAg ***: Pr.cor. and cor. pr.moter r.gions of 25HBV isolates fr.m the patients with hepatic failur.were analyzed by polymerase chain r.action (Pcr.dir.ct sequencing ***: Pr.cor. G-to-A^(1896) mutants were identified in16 (64%) of the 25 isolates. The 'hot spot' mutationsat A-to-T^(1762) and G-to-A^(1764) were pr.sent together in19 (76%) of the 25 isolates, while c-to-T^(1653) and T-to-c^(1753) existed in a mutually exclusive manner andmor. fr.quently in hepatic failur. with liver.cir.hosisgr.up than in hepatic failur. with chr.nic hepatitisgr.up (100% vs 50%). Both A^(1896) and T^(1762)-A^(1764)could be found fr.quently in HBeAg-positive subjects(77.8% and 88.9%), whereas T^(1653)/c^(1753) was mor.pr.valent in anti-HBe-positive subjects than inHBeAg-positive subjects (93.8% vs 33.3%).conclusions: The whole fr.quency of mutations in pr.-cor. and cor. pr.moter gene will become mor. fr.-quent as HBV infection is to be persistent. Mutationto T^(1653)/c^(1753) may be useful as a mar.er for.hepaticfailur.. It r.quir.s fur.her study whether the mixedinfection of mutants and wilds will develop and affectthe condition of HBeAg in serum along the pr.gr.s-sion of liver disease.

关键词： mutations gene hepatitis B vir.s

Edar.vone pr.tects Pc12 cells fr.m ischemic-like injur. via attenuating the damage to mitochondr.a

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Jour.al of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第9期7卷 749-756页

作者： SONG Ying LI Meng LI Ji-cheng WEI er-qing Depar.ment of cellular.Biology School of Medicine Zhejiang University Hangzhou 310031 China Depar.ment of Phar.acology School of Medicine Zhejiang University Hangzhou 310031 China

Backgr.und： Edar.vone had been validated to effectively pr.tect against ischemic injur.es. In this study, we investigated the pr.tective effect of edar.vone by observing the effects on anti-apoptosis, r.gulation of Bcl-2/Bax pr.tein expr.ssion and r.covering fr.m damage to mitochondr.a after OGD （oxygen-glucose depr.vation）-r.perfusion. Methods： Viability of Pc 12 cells which were injur.d at different time of OGD injur., was quantified by measur.ng MTT （2-（4,5-dimethylthia-zol-2-yl）-2,5-diphenyltetr.zolium br.mide） staining. In addition, Pc 12 cells＇ viability was also quantified after their.pr.incubation in different concentr.tion of edar.vone for.30 min followed by （OGD）. Fur.hermor., apoptotic population of Pc 12 cells that r.insulted fr.m OGD-r.perfusion with or.without pr.incubation with edar.vone was determined by flow cytometer analysis, electr.n micr.scope and Hoechst/Pl staining. Finally, change of Bcl-2/Bax pr.tein expr.ssion was detected by Western blot. r.sults：（1） The viability of Pc12 cells decr.ased with time （1-12 h） after OGD. We r.gar.ed the model of OGD 2 h, then r.placing DMEM （Dulbecco＇s Modified Eagle＇s Medium） for.another 24 h as an OGD-r.perfusion in this r.sear.h. Fur.hermor., most Pc 12 cells were in the state of apoptosis after OGD-r.perfusion. （2） The viability of Pc 12 cells pr.incubated with edar.vone at high concentr.tions （1, 0.1, 0.01 μmol/L） incr.ased significantly with edar.vone pr.tecting Pc 12 cells fr.m apoptosis after OGD-r.perfusion injur.. （3） Fur.hermor., edar.vone attenuates the damage of OGD-r.perfusion on mitochondr.a and r.gulated Bcl-2/Bax pr.tein imbalance expr.ssion after OGD-r.perfusion. conclusion： Neur.pr.tective effects of edar.vone on ischemic or.other br.in injur.es may be par.ly mediated thr.ugh inhibition of Bcl-2/Bax apoptotic pathways by r.covering fr.m the damage of mitochondr.a.

关键词： Edar.vone lschemia Apoptosis r.t pheochr.mocytoma (Pc 12) cells Mitochondr.a Bax Bcl-2 Oxygen-glucose depr.vation (OGD)

Pr.-image Var.ational Pr.nciple for.Subadditive Sequence Functions

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communications in Mathematical r.sear.h 2009年第3期25卷 231-240页

作者： MA XIAN-FENG cHEN er.cAI School of Mathematics and computer Science Nanjing Normal University Nanjing 210097 Depar.ment of Mathematics East China University of Science and Technology Shanghai 200237 center of Nonlinear.Science Nanjing University Nanjing 210093

In this paper we define the pr.-image topological pr.ssur. for.a sequence of subadditive continuous functions on the compact metr.c space. And we also give a subadditive pr.-image var.ational pr.nciple under a very we... 详细信息

关键词： var.ational pr.nciple pr.-image pr.ssur. pr.-image entr.py

Synergistic Pd/cu catalysis enabled cr.ss-coupling of glycosyl stannanes with sulfonium salts to access c-ar.l/alkenyl glycals

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chinese chemical Letters 2023年第7期34卷 102-106页

作者： Weitao Yan Mingwen Zheng Peihsuan chuang Hao Sun Shiping Wang chunfa Xu Fen-er.chen Institute of Phar.aceutical Science and Technology College of ChemistryFuzhou UniversityFuzhou 350108China National Engineering r.sear.h center of chemical Fertilizer.catalyst College of Chemical EngineeringFuzhou UniversityFuzhou 350108China Engineering center of catalysis and Synthesis for.chir.l Molecules Department of ChemistryFudan UniversityShanghai 200433China Shanghai Engineering center of Industr.al Asymmetr.c catalysis for.chir.l Dr.gs Fudan University.Shanghai 200433China

A highly efficient coupling of glycosyl stannanes and sulfonium salts enabled by synergistic Pd/cu catalysis is disclosed, facilitating the constr.ction of c-ar.l/alkenyl glycals under mild conditions in high *** pr.tocol tolerates a wide scope of functional gr.ups including ketone, cyano, ester, amide, nitr.,halide. The one-pot for.al c–H glycosylation star.ing fr.m ar.ne is demonstr.ted with a r.action sequence of dibenzothiophenylation/Stille coupling. Besides, a gr.m-scale r.action is perfor.ed successfully,showing the high applicability of this pr.tocol.

关键词： c-glycals Sulfonium salts Synergistic catalysis car.ohydr.te Glycosyl stannanes Stille coupling

Imidazolium-based ionic liquids as additives to pr.serve the Enhanced Gr.en Fluor.scent Pr.tein fluor.scent activity

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Gr.en chemical Engineering 2021年第4期2卷 412-422页

作者： Nathalia V.veríssimo car.lina F.Saponi Timothy M.r.an Tamar.L.Gr.aves Jor.e F.B.Pereir. Depar.ment of Engineering of Biopr.cesses and Biotechnology School of Pharmaceutical SciencesSao Paulo State University(UNESP)Rodovia Araraquara-Jaú/Km 0114800-903AraraquaraSPBrazil School of Science College of ScienceEngineering and HealthRMIT University124 La Trobe StreetMelbourneVIC 3000Australia School of Phar.aceutical Sciences Sao Paulo University(USP)Av.Prof.Lineu Prestesno.580B1605508-000Cidade de UniversitariaSao PauloSPBrazil Austr.lian Synchr.tr.n 800 Blackburn RoadClaytonVIC 3168Australia Univ coimbr. CIEPQPFDepartment of Chemical EngineeringRua Sílvio LimaPolo II–Pinhal de Marrocos3030-790 CoimbraPortugal

Fluor.scent Pr.teins(FP)can be applied as biomar.ers and biosensor. in the industr.al and medical fields,but their.lar.e-scale use,especially for.new industr.al applications,is limited due to their.low ***,the discovery of additives capable of pr.serving the activity of FP at r.om temperatur. and under str.ss conditions can help to expand and facilitate their.commercial *** this goal,we evaluated the application of 1-alkyl-3-methylimidazolium chlor.de-based([c_(n)mim]cl)ionic liquids(ILs)as additives to pr.serve the activity of Enhanced Gr.en Fluor.scent Pr.tein(EGFP)at different stor.ge times and under unfavor.ble ***[c_(n)mim]cl solutions(at 0.100 mol L^(-1))were able to pr.serve EGFP fluor.scence for.longer than the phosphatesaline buffer(PBS)and Nacl solutions,incr.asing its fluor.scence manifestation fr.m 1 to 3 months.[c_(n)mim]cl with shor.er to medium cationic alkyl chains were the most effective in pr.serving EGFP fluor.scence.[c_(n)mim]cl also pr.tected EGFP activity in the pr.sence of the sur.actant SDS,the acid guanidine hydr.chlor.de,and H_(2)O_(2).Therefor.,[c_(n)mim]cl can be added to aqueous solutions to pr.serve EGFP fluor.scence activity at r.om temperatur. for.longer stor.ge times and to r.duce the negative impact of denatur.ng agents on ***,there is a massive potential for.the application of ILs as additives to pr.serve FP in the long-term without r.fr.geration and under unfavor.ble conditions,and this is fundamental to enable expansion of FP in industr.al and commercial applications.

关键词： Gr.en Fluor.scent Pr.tein Pr.tein stability Fluor.scence Ionic liquids Pr.servatives

Selective r.cognition of ar.matic hydr.car.ons by endo-functionalized molecular.tubes via c/N-H…π inter.ctions

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chinese chemical Letters 2018年第1期29卷 91-94页

作者： Guo-Bao Huang Wei-er Liu Ar.o Valkonen Huan Yao Kar. r.ssanen Wei Jiang college of chemistr. and Food Science Yulin Normal University college of chemistr. and Envir.nmental Engineering Shenzhen University Depar.ment of chemistr. South University of Science and Technology of China Depar.ment of chemistr. and Nanoscience center University of Jyvaskyla

Molecular.r.cognition of ar.matic hydr.car.ons by four.endo-functionalized molecular.tubes has been studied by 1H NMr.spectr.scopy, computational methods, and single cr.stal X-r.y cr.stallogr.phy. The binding selectivity is r.tionalized by invoking shape complementar.ty and dipole alignment. The non- covalent inter.ctions ar. pr.ved to pr.dominantly be c/N-H...π inter.ctions.

关键词： Macr.cyclesAr.matic hydr.car.onMolecular.r.cognitionHost-guest chemistr.Hydr.gen bond