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Asian Journal of Andrology 2021年第1期23卷 118-119页

作者： Peng Li er-lei zhi Chen-Cheng Yao Shu-Jie Xia Zheng Li Department of Andrology Center for Men's HealthUrologic Medical CenterShanghai General HospitalShanghai Jiad Tong UniversityShanghai 200080China Department of Urology Shanghai General Haspital of Nanjing Medical UniversityShanghai 200080China

Dear Editor,Microsurgical vasovasostomy,most commonly performed for vasectomy reversal,remains the most successful procedure for restoring patency to the vas deferens with the return of sperm to the *** the excellent results depend on the surgeons skill and technique,optimal vision and ergonomics are also crucial factors contributing to the achievement of good results during male infertility microsurgery.

关键词： return image system

Seminal plasma miR-192a： a biomarker predicting successful resolution of nonobstructive azoospermia following varicocele repair

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Asian Journal of Andrology 2018年第4期20卷 396-399页

作者： er-lei zhi Guo-Qing Liang Peng Li Hui-Xing Chen Ru-Hui Tian Peng Xu Zheng Li Department of Andrology Center for Men's Health Department of ART Institute of Urologic Medical Center Shanghai General Hospital Shanghai Key Lab of Reproductive Medicine Shanghai Jiao Tong University 100 Haining Road Shanghai 200080 China Department of Andrology Seventh People's Hospital of Shangha University of TCM Shanghai 200000 China Oriental Jinghua Hospital of Shenyang Department of ART 156 Nanjing Road Shenyang 110005 China

This study was performed to investigate a potential marker for the presence of spermatozoa in the ejaculate following varicocelectomy in Chinese men with nonobstructive azoospermia and varicoceles. The micro-RNA （miR）-192a levels in seminal plasma and testicular tissue were evaluated by quantitative real-time polymerase chain reaction from 60 men with nonobstructive azoospermia and varicoceles （Group A： 27 men with spermatozoa found in the ejaculate after surgery; Group B： 33 men without spermatozoa found in the ejaculate after surgery） and 30 controls. The seminal plasma and testicular tissue miR-192a levels were higher in Group B than in Group A and the controls （P〈 0.001）, and there was no significant difference between Group A and the controls （P〉 0.05）. Apoptosis and proliferation assays with miR mimics and inhibitors showed that miR-192a induced GC-2 cell apoptosis through the activation of Caspase-3 protein. Thus, seminal plasma miR-192a appears to be a potential marker for successfully indicating spermatozoa in the ejaculate following microsurgical varicocelectomy in men with nonobstructive azoospermia and varicoceles. Seminal plasma miR-192a may be a useful clinical marker for prescreening to determine which patients with nonobstructive azoospermia and varicoceles would benefit from varicocelectomy.

关键词： miR-192a nonobstructive azoospermia seminal plasma varicocelectomy

Vasectomy reversal in China during the recent decade:insights from a multicenter retrospective investigation

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Asian Journal of Andrology 2023年第3期25卷 416-420页

作者： Li-Juan Ren Rui-zhi Xue Zi-Qiang Wu er-lei zhi Wei Li Liang Huang Xu-Yu Xiang Dao-Yuan Li Xue-Ming Lin Department of Urology First Hospital of Shanxi Medical UniversityTaiyuan 030000China Department of Urology Xiangya Hospital of Central South UniversityChangsha 410000China Department of Urology Shanghai General HospitalShanghai 200000China Department of Urology Second Hospital of Tianjin Medical UniversityTianjin 300000China Department of Urology Zhujiang Hospital of Southern Medical UniversityGuangzhou 510000China Department of Urology First Affiliated Hospital of Nanchang UniversityNanchang 330000China Department of Urology Hainan General HospitalHaikou 570100China

To date,there is little information about the demography of vasectomy reversal(VR)patients or the factors currently influencing VR effectiveness in China,especially after the universal two-child policy was released in *** this research,demographic data and perioperative medical records of VR patients were extracted from seven major hospitals in different provinces or municipalities of ***,a telephone survey of the patients was conducted to collect follow-up ***,448 VR cases from the past 13 years were *** results were analyzed by stratified comparison to investigate factors that can influence postoperative vas deferens patency and pregnancy *** statistical methods were used,and all of the protocols were approved by the Ethics Committees of the institutes in this *** results showed that the annual operation volume of VR quadrupled after the two-child policy was *** and a long duration of vasectomy were significantly associated with a lower patency rate.A follow-up survey showed that the general postoperative pregnancy rate was 27.2%.For female partners over the age of 35 years,the postoperative pregnancy rate showed a more severe decline,but only 35.5%of them had been given a fertility examination before their husbands’VR *** work revealed that more patients in China have been demanding VR in recent ***-quality microsurgery and a short duration of vasectomy are crucial for restoring patency by *** andrologists should perform a preoperative fertility evaluation of the patients’female partners.

关键词： demography microsurgery multicenter vasectomy reversal

Relationship between self-reported residential indoor remodeling and semen quality： a case-control study

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Asian Journal of Andrology 2015年第6期17卷 981-984,I0009,I0010页

作者： Mao-Hua Miao Zheng Li De-Kun Li Bei Yan Hong Liang er-lei zhi Hong-Wei Du Wei Yuan Department of Reproductive Epidemiology and Social Science National Population and Family Planning Key Laboratory of Contraceptive Drugs and Devices ShanghaiI~stitue of Planned Pa[enthood Rese.arch Shanghai China Department of Urology Shanghai Human Sperm Bank Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China Division of Reseaich Kaiser Foundation Research Institute Kaiser Permanente Northern California CA USA.

The present study examined the association between residential indoor remodeling and poor semen quality. Sperm donors aged 18-45 years old were recruited in Shanghai, China. Semen specimens were collected and analyzed. An in-person interview was conducted to obtain information on the history of indoor remodeling and potential confounders. A total of 70 participants with abnormal semen quality （case group） and 68 controls were examined. A total of 20 subjects reported indoor remodeling in the recent 24 months, and among them 17 subjects reported indoor remodeling in the recent 12 months. Compared with participants with no history of indoor remodeling, participants with a history of indoor remodeling in the recent 24 months were more than three times as likely to have poor sperm quality （adjusted odds ratio = 3.8, 95% confidence interval： 1.3-12.0） after controlling for potential confounders. The association was strengthened when the analysis was restricted to those who had indoor remodeling in the recent 12 months. Our findings provide preliminary evidence that indoor remodeling has an adverse effect on semen quality.

关键词： case-control study chemicals health effect indoor remodeling semen quality

Successful microsurgical vasoepididymostomy for a case of cryptozoospermia

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Asian Journal of Andrology 2022年第4期24卷 436-437页

作者： Ru-Hui Tian Yu-Hua Huang Hui-Xing Chen Peng Li er-lei zhi Chen-Cheng Yao Chao Yang Zheng Li Department of Andrology The Center for Men's HealthUrologic Medical CenterShanghai Key Laboratory of Reproductive MedicineShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China

Dear Editor,Cryptozoospermia is defined as the apparent absence of spermatozoa from fresh semen samples,but they *** found in centrifuged pellets.1 It is usually caused by a spermatogenic disorder,while cryptozoospermia associated with seminal duct obstruction is less common.2 Because of the extremely low sperm counts in semen samples,men with cryptozoospermia are often reliant on intracytoplasmic sperm injection(ICSI)for paternity.

关键词： injection crypt obstruction

3D digital image microscope system-assisted vasovasostomy and vasoepididymostomy in rats

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Asian Journal of Andrology 2021年第4期23卷 396-399页

作者： Peng Li Na-Chuan Liu er-lei zhi Chen-Cheng Yao zhi-Liang Zhao zhi-Yong Yu Qi-Meng Li Yu-Hua Huang Jie-Chang Ju Wen-Bin Huang Husanjan Rozi zhi-Yong Ji San-Wei Guo Ru-Hui Tian Zheng Li Shanghai General Hospital Shanghai Jiao Tong University School of MedicineShanghai 200080China Gansu Provincial Hospital of Traditional Chinese Medicine Lanzhou 730050China Weifang Traditional Chinese Hospital Weifang 250200China The Third Affiliated Hospital of Wenzhou Medical University Wenzhou 325000China Zhengzhou Central Hospital Affiliated with Zhengzhou University Zhengzhou 450007China Wenzhou Integrated Traditional Chinese Wenzhou 325000China Kashgar District Second People's Hospital Kashgar 844000China Shanghai General Hospital of Nanjing Medical University Shanghai 200080China Shanghai Tenth People's Hospital Tongji UniversityShanghai 200072China

Optimal vision and ergonomics are essential factors contributing to the achievement of good results during *** three-dimensional(3D)digital image microscope system with a better 3D depth of field can release strain on the surgeon's neck and back,which can improve outcomes in *** report a randomized prospective study of vasoepididymostomy and vasovasostomy using a 3D digital image microscope system(3D-DIM)in rats.A total of 16 adult male rats were randomly divided into two groups of 8 each:the standard operating microscope(SOM)group and the 3D-DIM *** outcomes measured included the operative time,real-time postoperative mechanical patency,and anastomosis ***,a user-friendly microscope score was designed to evaluate the ergonomic design and equipment characteristics of the *** were no differences in operative time between the two *** real-time postoperative mechanical patency rates were 100.0%for both *** percentage of vasoepididymostomy anastomosis leakage was 16.7%in the SOM group and 25.0%in the 3D-DIM group;however,no vasovasostomy anastomosis leakage was found in either *** terms of the ergonomic design,the 3D-DIM group obtained better scores based on the surgeon's feelings;in terms of the equipment characteristics,the 3D-DIM group had lower scores for clarity and higher scores for flexibility and *** on our randomized prospective study in a rat model,we believe that the 3D-DIM can improve surgeon comfort without compromising outcomes in male infertility reconstructive microsurgery,so the 3D-DIM might be widely used in the future.

关键词： microsurgery three-dimensional digital image microscope system vasoepididymostomy vasovasostomy

Microsurgical subinguinal varicocelectomy with spermatic cord double traction and vein stripping

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Asian Journal of Andrology 2020年第2期22卷 208-212页

作者： Ru-Hui Tian Liang-Yu Zhao Hui-Xing Chen Chao Yang Peng Li Yu-Hua Huang Zhong Wan er-lei zhi Chen-Cheng Yao Zheng Li Department of Andrology Center for Men’s HealthDepartment of ARTUrologic Medical CenterShanghai General HospitalShanghai Key Lab of Reproductive MedicineShanghai Jiao Tong UniversityShanghai 200080China

We retrospectively reviewed data for 286 patients with varicocele who underwent microsurgical subinguinal varicocelectomy from March 2015 to May 2017 in Shanghai General Hospital(Shanghai,China).In this surgical approach,the testis was delivered,and the gubernacular and external cremasteric veins were *** addition,the spermatic cord was delivered downward with continuous double traction away from the external *** remaining procedure was similar to the conventional *** followed patients for at least 3 months and evaluated postoperative semen parameters,pain symptoms,and *** excluded data for 32 men due to inadequate follow-up(ermia,121 had nonobstructive azoospermia,and 60 had symptomatic *** progressive sperm counts increased in the oligoasthenospermic patients from a median preoperative value of 9.15×10^6 ml^?1 to 25.33×10^6 ml^?1(n=34),and 35.6%(26/73)initially oligoasthenospermic men contributed to unassisted *** returned to the ejaculate in 12.4%(15/121)azoospermia *** patients with scrotal pain(n=60),43(71.7%)reported complete resolution of pain,16(26.7%)reported partial resolution,and 1(1.7%)reported no *** patients experienced varicocele *** doubletraction strategy avoids opening the external oblique aponeurosis,and results in less damage and faster *** addition,the stripping strategy eliminates potential damage to the testis caused by the varicose *** results showed that microsurgical subinguinal varicocelectomy using spermatic cord double traction in conjunction with testicular delivery for vein stripping is a safe and effective approach for varicocele repair.

关键词： azoospermia low sperm count sperm motility spermatic cord varicocele

Efficacy of stepwise mini-incision microdissection testicular sperm extraction for nonobstructive azoospermia with varied etiologies

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Asian Journal of Andrology 2023年第5期25卷 621-626页

作者： Yu-Xiang Zhang Chen-Cheng Yao Yu-Hua Huang Peng Li er-lei zhi Zi-Jue Zhu Jian-Xiong Zhang Fu-Jun Zhao Zheng Li Ru-Hui Tian Department of Andrology The Center for Men's HealthUrologic Medical CenterShanghai Key Laboratory of Reproductive MedicineShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China

Stepwise mini-incision microdissection testicular sperm extraction(mTESE)is a procedure that attempts to minimize testicular ***,the mini-incision approach may vary in patients with different ***,we performed a retrospective analysis of 665 men with nonobstructive azoospermia(NOA)who underwent stepwise mini-incision mTESE(Group 1)and 365 men who underwent standard mTESE(Group 2).The results showed that the operation time(mean±standard deviation)for patients with successful sperm retrieval in Group1(64.0±26.6min)was significantly shorter than that in Group2(80.2±31.3min),with Perm retrieval rate(SRR)was 23.1%in our study,and there was no significant difference between Group 1 and Group 2(P>0.05),even when the etiologies of NOA were taken into *** results of consecutive multivariate logistic regression analysis(odds ratio[0R]:0.57;95%confidence interval[Cl]:0.38-0.87;P=0.009)and receiver operating characteristic(ROC)analysis(area under the ROC curve[AUC]=O.628)showed that preoperative anti-Mullerian hormone(AMH)level in idiopathic NOA patients was a potential predictor for surgical outcomes after initial three small incisions made in the equatorial region without sperm examined under an operating microscope(Steps 2-4).In conclusion,stepwise mini-incision mTESE is a useful technique for NOA patients,with comparable SRR,less surgical invasiveness,and shorter operation time compared with the standard *** AMH levels may predict successful sperm retrieval in idiopathic patients even after a failed initial mini-incision procedure.

关键词： etiology male infertility nonobstructive azoospermia standard mTESE stepwise mini-incision mTESE

Clinical features and microsurgical reconstruction of congenital unilateral absence of the vas deferens with obstructive azoospermia:a tertiary care center experience

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Asian Journal of Andrology 2023年第1期25卷 73-77页

作者： Yi-Hong Zhou Jian-Jun Dong er-lei zhi Chen-Cheng Yao Yu-Hua Huang Ru-Hui Tian Hui-Xing Chen Ying-Bo Dai Yu-Xin Tang Na-Chuan Liu Hui-Rong Chen Fu-Jun Zhao Zheng Li Peng Li Department of Andrology The Center for Men's HealthUrologic Medical CenterShanghai General HospitalShanghai Jiaotong University School of MedicineShanghai 200080China Department of Urology The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000China Department of Andrology Jiaozuo Maternity and Infant Health HospitalJiaozuo 454000China

Patients with congenital unilateral absence of the vas deferens(CUAVD)manifest diverse symptoms from normospermia to *** for CUAVD patients with obstructive azoospermia(OA)is complicated,and there is a lack of relevant *** this study,we describe the clinical features and evaluate the treatments and outcomes of CUAVD patients with *** December 2015 to December 2020,33 patients were diagnosed as CUAVD with OA in Shanghai General Hospital(Shanghai,China).Patient information,ultrasound findings,semen analysis,hormone profiles,and treatment information were collected,and the clinical outcomes were *** 33 patients,29 patients were retrospectively ***(VE)or cross VE was performed in 12 patients,the patency rate was 41.7%(5/12),and natural pregnancy was achieved in one of the *** other 17 patients underwent testicular sperm extraction as the distal vas deferens(contralateral side)was *** findings showed that VE or cross VE remains an alternative treatment for CUAVD patients with OA,even with a relatively low rate of patency and natural pregnancy.

关键词： congenital unilateral absence of the vas deferens natural pregnancy obstructive azoospermia patency vasoepididymostomy

Modified stepwise mini-incision microdissection testicular sperm extraction:a useful technique for patients with a history of orchidopexy affected by non-obstructive azoospermia

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第1期21卷 87-92页

作者： Peng LI Chen-cheng YAO er-lei zhi Yuan XU Zhong WAN Ying-chuan JIANG Yu-hua HUANG Yue-hua GONG Hui-xing CHEN Ru-hui TIAN Chao YANG Liang-yu ZHAO Zheng LI Department of Andrology Center for Men’s HealthUrologic Medical CenterShanghai General HospitalShanghai Jiao Tong UniversityShanghai 200080China Department of Urology Shanghai General Hospital of Nanjing Medical UniversityShanghai 200080China Reproductive Medicine Center Shanghai General HospitalShanghai Jiao Tong UniversityShanghai 200080China Department of Urology Zhoushan HospitalZhoushan 316021China

Non-obstructive azoospermia(NOA),which is defined as the absence of spermatozoa in the ejaculate secondary to impaired spermatogenesis within the testis,may be caused by a variety of etiologies,including varicocele-induced testicular damage,cryptorchidism,prior testicular torsion,post-pubertal mumps orchitis,gonadotoxic effects from medications,genetic abnormalities,chemotherapy/radiation,and other unknown causes currently classified as idiopathic(Cocuzza et al.,2013).The microdissection testicular sperm extraction(micro-TESE)technique involves a meticulous microsurgical exploration of the testicular parenchyma to identify and selectively extract larger seminiferous tubules that carry a higher probability of complete spermatogenesis(Schlegel,1999).

关键词：非梗阻性无精子症隐睾睾丸显微取精术睾丸下降固定术精子获取