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Severe splenic rupture after colorectal endoscopic submucosal dissection

World Journal of Gastroenterology 2014年第28期20卷 9618-9620页

作者： Alberto Herreros de Tejada Luis Giménez-Alvira enrique van den brule Rosario Sánchez-Yuste Pilar Matallanos Esther Blázquez Jose L Calleja Luis E Abreu Department of Gastroenterology IDIPHIMPuerta de Hierro University Hospital Department of Surgery IDIPHIMPuerta de Hierro University Hospital Department of Radiology IDIPHIMPuerta de Hierro University Hospital Department of Pathology IDIPHIMPuerta de Hierro University Hospital

Splenic rupture(SR)after colonoscopy is a very rare but potentially serious *** diagnosis is common,and may increase morbidity and mortality *** is no clear relation between SR and difficult diagnostic or therapeutic procedures,but it has been suggested that loop formation and excessive torquing might be risk *** is a case of a65-year-old woman who underwent endoscopic submucosal dissection(ESD)for lateral spreading tumor in the descending colon,and 36 h afterwards presented symptoms and signs of severe hypotension due to *** splenectomy was completed and the patient recovered *** ESD is usually a long and position-demanding technique,implying torquing and loop *** our knowledge this is the first case of SR after colorectal ESD reported in the *** performing colorectal ESD in the left colon must be aware of this potential complication.

关键词： Splenic rupture Endoscopic submucosal dissection Colorectal neoplasm Colonoscopy Splenectomy

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Tuf m RNA rather than 16S r RNA is associated with culturable Staphylococcus aureus

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World Journal of Clinical Infectious Diseases 2015年第4期5卷 86-93页

作者： Anne JM Loonen Petra FG Wolffs Maikel de Bresser Maurice Habraken Cathrien A Bruggeman Mirjam HA Hermans Adriaan JC van den brule Laboratory for Molecular Diagnostics Department of Medical Microbiology and Pathology Jeroen Bosch Hospital Department of Medical Molecular Diagnostics Fontys University of Applied Sciences Department of Medical Microbiology CAPHRI Maastricht University Medical Centre

AIM: To study the presence of various nucleic acids targets of Staphylococcus aureus(S. aureus) during bacterial growth and antibiotic induced killing in relation to ***: S. aureus was cultured to log phase and spiked in Todd Hewitt(TH) broth and whole blood of healthy human volunteers. Viability of S. aureus after flucloxacillin treatment(0, 1, 3 and 6 d) was assessed by culture on bloodagar plates. DNA and RNA were isolated from 200 μL. c DNA synthesis was performed by using random primers. The presence of S. aureus DNA, r RNA, and m RNA were determined by real-time polymerase chain reaction of the 16 S r DNA and tuf gene(elongation factor Tu).RESULTS: S. aureus spiked in TH broth without antibiotics grew from day 0-6 and DNA(tuf and 16S), and 16 S r RNA remained detectable during this whole period. During flucloxacillin treatment S. aureus lost viability from day 3 onwards, while the 16 S r RNA-gene and its RNA transcripts remained detectable. DNA andr RNA can be detected in flucloxacillin treated S. aureus cultures that do not further contain culturable ***, tuf m RNA became undetectable from day 3onwards. Tuf m RNA can only be detected from samples with culturable bacteria. When spiking S. aureus in whole blood instead of broth no bacterial growth was seen, neither in the absence nor in the presence of flucloxacillin. Accordingly, no increase in DNA and RNA levels of both 16 S r DNA and the tuf gene were detected. CONCLUSION: Tuf m RNA expression is associated with culturable S. aureus and might be used to monitor antibiotic effects.

关键词： Bloodstream infection Staphylococcus aureus Viability mRNA Polymerase chain reaction Sepsis Molecular diagnostics Blood

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