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Puma （Puma concolor） epididymal sperm morphometry

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Asian Journal of Andrology 2016年第6期18卷 879-881页

作者： Hernan Cucho Virgilio Alarcon Cesar Ordonez enrique Ampuero Aydee Meza Caries Solere Zootechny School National University of San Atonio Abad del Cusco Postal code 921 Cusco Peru Functional Biology and Physical Anthropology department University of Valencia C/. Dr Moliner 50 46100 Burjassot Valencia Spain Proiser R+D S.L. Scientific Park of the University of Valencia 46980 Paterna Spain

The Andean puma （Puma concolor） has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component （PC） analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species.

关键词： principal component analysis puma sperm morphometry subpopulation wild animal

Nature and evolution of the lower crust under central Spain:Inferences from granulite xenoliths(Calatrava Volcanic Field-Spanish central system)

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Geoscience Frontiers 2023年第3期14卷 10-32页

作者： David Orejana Carlos Villaseca Marlina A.Elburg enrique Merino-Martínez Javier García Serrano departamento de Mineralogía y Petrología Universidad Complutense de MadridSpain departamento de Dinámica Terrestre y Observación de la Tierra Instituto de Geociencias IGEO(UCMCSIC)MadridSpain department of Geology University of JohannesburgSouth Africa Instituto Geológico y Minero de España–Consejo Superior de Investigaciones Científicas CN IGME–CSIC

So far,the nature and evolution of the lower crust under central Spain have been constrained mainly on the basis of a heterogeneous suite of granulite xenoliths from the Spanish Central System(SCS).In recent years,ultramafic volcanics from the Calatrava Volcanic Field(CVF)have also provided deep-seated crustal xenoliths which have not been studied in *** data,combining mineral,whole-rock and isotopic geochemistry with U–Pb–Hf isotope ratios in zircons from the CVF and SCS xenoliths,highlight the felsic composition of the lower crust under central Iberia.A number of the Calatrava xenoliths represents Variscan igneous protoliths,which are a minor population in the SCS,and were likely formed by crystallisation of intermediate and felsic melts in the lower crust during the Variscan orogeny(leucodiorite protolith age of 314±3 Ma and leucogranite protolith age of 308±2.5 Ma).U–Pb data of metamorphic zircons show that granulite-facies metamorphism mainly occurred from 299 to 285 Ma in both *** ages are slightly younger than those of granitic intrusions that could be genetically related to the granulitic residue,which points to a main role of U–Pb isotope resetting in lower crustal zircons during HT or UHT *** zircon U–Pb–Hf isotopic ratios support the idea that the lower crust in central Iberia consists mainly of Ordovician–Neoproterozoic metaigneous and metasedimentary rocks associated with the Cadomian continental arc of northern *** rocks provide evidence of mixing between juvenile magmas and an enriched crustal component,ultimately extracted from an Eburnean *** more evolved components present in detrital zircons are likely related to recycling of Archean crust derived from North Africa cratonic terranes.

关键词： Granulite xenoliths U-Pb geochronology Lu-Hf isotopes Calatrava Volcanic Field Spanish central system

Correlations between corneal and optic nerve head variables in healthy subjects and patients with primary open angle glaucoma

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International Journal of Ophthalmology(English edition) 2015年第6期8卷 1156-1161页

作者： Federico Saenz-Frances Luis Janez Lara Borrego-Sanz Clara Berrozpe-Villabona Jose Maria Martinez-de-la-Casa Laura Morales-Fernandez Julian Garcia-Sanchez enrique Santos-Bueso Julian Garcia-Feijoo department of Ophthalmology Clinico San Carlos HospitalComplutense University of Madrid Technology of Knowledge Institute Complutense University of Madrid

AIMTo correlate corneal variables (determined using the Pentacam) with optic nerve head (ONH) variables determined using the Heidelberg retina tomograph (HRT) in healthy subjects and patients diagnosed with primary open angle glaucoma (POAG).METHODSMeasurements were made in 75 healthy eyes and 73 eyes with POAG and correlations examined through Pearson correlation coefficients between the two sets of variables in the two subject groups. The corneal variables determined were corneal volume (CVol), central corneal thickness (CCT), overall corneal thickness (OvCT), the mean thickness of a circular zone centered at the corneal apex of 1 mm radius (zone I) and the mean thickness of several concentric rings, also centered at the apex until the limbus, each of 1 mm width (zones II to VI respectively). The ONH variables were determined using the *** following pairs of variables were correlated in the control group: CCT-disc area (DAr) (-0.48; Pdetected between central and paracentral corneal thickness and optic disc area. In contrast, the POAG patients showed significant negative correlation between central and paracentral corneal thickness and the cup-disc ratio and CSM, and positive correlation between peripheral corneal thickness and HVC.

关键词： glaucoma optic nerve head corneal thickness

In vivo optical quality of posterior-chamber phakic implantable collamer lenses with a central port

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Eye and Vision 2021年第5期8卷 295-302页

作者： Robert Montés-Micó Francisco Pastor-Pascual enrique Artiaga-Elordi Ramón Ruiz-Mesa Pedro Tañá-Rivero Optics and Optometry&Vision Sciences department University of Valenciac/o Dr Moliner 5046100 ValenciaSpain Oftalvist Clinic ValenciaSpain

Background:The aim of this review is to summarize the optical quality results in patients following the implantation of the V4c implantable collamer lens with a central port(ICL,STAAR Surgical Inc.).Main text:A literature search in several databases was carried out to identify those publications,both prospective,retrospective and/or comparative with other refractive surgery procedures,reporting optical outcomes of patients who were implanted with the V4c ICL model.A total of 17 clinical studies published between 2012 and 2021 were included in this review.A detailed analysis of the available data was performed including number of eyes,follow-up and preoperative spherical ***,the review focused on several optical parameters including higher-order aberrations(HOAs),modulation transfer function(MTF)cut-off frequency and Strehl *** review encompassed a total of 817 eyes measured using different optical devices based on Hartmann-Shack,retinal image quality measurement and ray-tracing technologies at different ***:The outcomes found in this review lead us to conclude that the ICL V4c model provides good optical quality,by means of different metrics,when implanted.

关键词： Implantable collamer lens Phakic Optical quality Higher order aberrations Modulation transfer function

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dependence of dynamic contour and Goldmann applanation tonometries on peripheral corneal thickness

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International Journal of Ophthalmology(English edition) 2017年第10期10卷 1521-1527页

作者： Federico Saenz-Frances Claudia Sanz-Pozo Lara Borrego-Sanz Luis Ja?ez Laura MoralesFernandez Jose Maria Martinez-de-la-Casa Julian Garcia-Sanchez Julian Garcia-Feijoo enrique Santos-Bueso Ophthalmology department Clinico Universitario San Carlos HospitalComplutense University Institute of Knowledge Technology Complutense University

AIM：To determine the effects of peripheral corneal thickness（PCT）on dynamic contour tonometry（DCT）and Goldmann applanation tonometry（GAT）.METHODS：A cross-sectional *** created a software which calculates the corneal contour（CC）as a function of the radius from the corneal apex to each pixel of the *** software generates a central circumference with a radius of 1 mm and the remainder of the cornea is segmented in 5 rings concentric with corneal apex being its diameter not constant around the corneal circumference as a consequence of the irregular CC but keeping constant the diameter of each ring in each direction of the *** was determined as the mean thickness of the most eccentric *** weighted scatterplot smoothing（LOWESS）regression was used to determine the pattern of the relationship between PCT and both DCT and GAT ***,two multivariable linear regression models were *** each of them,the dependant variable was intraocular pressure（IOP）as determined using GAT and DCT *** both of the models the predictive variable was PCT though LOWESS regression pattern was used to model the relationship between the dependant variables and the predictor *** and sex were also introduced control variables along with their first-degree interactions with *** outcome measures include amount of IOP variation explained through regression models（R2）and regression coefficients（B）.RESULTS：Subjects included 109 eyes of 109 healthy *** regression suggested that a 2nd-degree polynomial would be suitable to model the relationshipbetween both DCT and GAT with *** PCT was introduced in both models as a linear and quadratic *** age nor sex nor interactions were statistically significant in both *** GAT model,R2was 17.14%（F=9.02;P=0.0002）,PCT linear term B was-1.163（95%CI：-1.163,-0.617）.PCT quadratic term B was 0.00081（95%CI：0.00043,0.00118）.For DCT model R2was 14.28%（F=9.29;P=0.0002

关键词： Goldmann applanation tonometry dynamic contour tonometry peripheral corneal thickness corneal thickness

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基于振动测试的张弦结构拉索索力识别

振动与冲击 2016年第23期35卷 148-152,184页

作者：李素贞 LAPUERTA enrique Cavero 同济大学土木工程学院上海200092 Polytechnic University of Catalonia Universitat Pol itecnica de Catalunya Barcelona Spain

张弦结构的拉索索力识别对拉索甚至整个结构的性态鉴定和可靠性评估具有重要意义。针对张弦结构中拉索索力识别应用,选取了5种基于振动测试的解析方法——3种传统的索力实用计算公式和2种新发展的边界不确定的索力识别方法,比较分析了... 详细信息

张弦结构的拉索索力识别对拉索甚至整个结构的性态鉴定和可靠性评估具有重要意义。针对张弦结构中拉索索力识别应用,选取了5种基于振动测试的解析方法——3种传统的索力实用计算公式和2种新发展的边界不确定的索力识别方法,比较分析了不同方法的优点和不足之处,探讨了索的边界条件、抗弯刚度和长细比的影响,确立了各种方法的适用范围和使用条件。分析结果表明:传统索力实用计算公式仅在边界条件比较明确(简支或固定)的情况下适用;当拉索的边界条件不确定时,宜采用Euler梁方法和Timohenko梁方法进行索力识别;当拉索的长细比<20时,宜采用Timohenko梁方法;弦理论、Shimada方法和Zui方法必须采用局部模态;Euler梁方法和Timohenko梁方法既可采用局部模态,也可采用整体模态。

关键词：索力识别张弦结构振动测试

Energetic-protein supplementation in the last 60 days of gestation improves performance of beef cows grazing tropical pastures

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Journal of Animal Science and Biotechnology 2018年第1期9卷 208-216页

作者： Aline Gomes da Silva Mário Fonseca Paulino Edenio detmann Henrique Jorge Fernandes Lincoln da Silva Amorim Román enrique Maza Ortega Victor Valério de Carvalho Josilaine Aparecida da Costa Lima Felipe Henrique de Moura Mariana Benevides Monteiro Jéssika Almeida Bitencourt Universidade Federal de Vicosa VicosaMinas Gerais 36570-000Brazil Universidade Federal de Mato Grosso do Sul Campo GrandeMato Grosso do Sul 79074-460Brazil Universidade Estadual de Mato Grosso do Sul AquidauanaMato Grosso do Sul 79200-000Brazil Biotran Biotecnologia e Treinamento em Reproducao Animal AlfenasMinas Gerais 37130-000Brazil Universidade Federal do Acre Rio BrancoAcre 69920-900Brazil

Background： Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments： control, which received no supplement; supplementation for the last 30 d of gestation（30-d; 3.0 kg/d）; supplementation for the last 60 d of gestation（60-d; 1.5 kg/d）; or supplementation for the last 90 d of gestation（90-d; 1.0 kg/d）. All supplemented treatments received the same total amount of supplement throughout the experiment： 90 kg（20% of crude protein）. A second experiment（Experiment 2） was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d ***： There was a linear effect of the number of days of supplementation on calving body weight（BW; P 〈 0.05）and a quadratic effect on BW change from parturition to d 31 post-calving（P 〈 0.05）, with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW（P 〉 0.10）. A significant linear effect on interval from parturition to conception（P 〈 0.05） was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility（P 〉 0.10）. Nitrogen excreted through urine tended to increase linearly with the level of supplementation（P 〈 0.10）.Conclusion： Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of

关键词： Flushing Nutrition Parturition Reproduction

The Genome of Cucurbita argyrosperma (Silver- Seed Gourd) Reveals Faster Rates of Protein- Coding Gene and Long Noncoding RNA Turnover and Neofunctionalization within Cucurbita

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Molecular Plant 2019年第4期12卷 506-520页

作者： Josue Barrera-Red on do enrique Ibarra-Laclette Alejandra Vazquez-Lobo Yocelyn T. Gutierrez-Guerrero Guillermo Sanchez de la Vega Daniel Pinero Salvador Montes-Hernandez Rafael Lira-Saade Luis E. Eguiarte departamento de Ecologia Evolutiva Instituto de Ecologia Universidad Nacional Aut6noma de Mexico Circuito Exterior s/n Anexo al Jardin Bot合nico 04510 Ciudad de Mexico Mexico departamento de Estudios Moleculares Avanzados Instituto de Ecologia A.C. Carretera Antigua a Coatepec No. 351 Col. El Haya. C.P. Xalapa Veracruz 91070 Mexico Centro de Investigaciones en Biodiversidad y Conservacion Universidad Autdnoma del Estado de Morelos Av. Universidad 1001 Col. Chamilpa Cuernavaca Morelos 62209 Mexico Campo Experimental Bajio Instituto Nacional de In vestigaciones Forestales Agricolas y Pecuarias (INIFAP) Km 6.5 Carretera Celaya-San Miguel de Allen de Celaya Guanajuato 38110 Mexico UBIPRO Facultad de Estudios Superiores Iztacala Universidad Nacional Autdnoma de Mexico Av. de los Barrios #1 Col. Los Reyes Iztacala Tlanepantla Edo. de Mex 54090 Mexico

Whole-genome duplications are an important source of evolutionary novelties that change the mode and tempo at which genetic elements evolve within a genome. The Cucurbita genus experienced a wholegenome duplication around 30 million years ago, although the evolutionary dynamics of the coding and noncoding genes in this genus have not yet been scrutinized. Here, we analyzed the genomes of four Cucurbita species, in eluding a newly assembled genome of Cucurbita argyrosperma, and compared the gene con tents of these species with those of five other members of the Cucurbitaceae family to assess the evolutionary dynamics of protein-coding and long intergenic noncoding RNA (lincRNA) genes after the genome duplication. We report that Cucurbita genomes have a higher protein-coding gene birth-death rate compared with the genomes of the other members of the Cucurbitaceae family. C. argyrosperma gene families associated with pollination and transmembrane transport had significantly faster evolutionary rates. lincRNA families showed high levels of gene turnover throughout the phylogeny, and 67.7% of the lincRNA families in Cucurbita showed evidence of birth from the neofunctionalization of previously existing protein-coding genes. Collectively, our results suggest that the whole-genome duplication in Cucurbita resulted in faster rates of gene family evolution through the neofunctionalization of duplicated genes.

关键词： Cucurbita argyrosperma, comparative genomics molecular evolution neofunctionalization long noncoding RNA whole-genome duplication

Risk prediction model for cutaneous squamous cell carcinoma in adult cardiac allograft recipients

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World Journal of Transplantation 2021年第3期11卷 54-69页

作者： Nandini Nair Zhiyong Hu Dongping Du enrique Gongora Division of Cardiology Department of Internal MedicineTexas Tech Health Sciences CenterLubbockTX 79430United States department of Industrial Manufacturing and Systems EngineeringTexas Tech UniversityLubbockTX 79409United States department of Cardiothoracic Surgery University of Alabama at BirminghamBirminghamAL 35233United States

BACKGROUND Heart transplant recipients are at higher risk of developing skin cancer than the general population due to the long-term immunosuppression *** has been reported as one of the major causes of morbidity and mortality for patients after heart *** different types of skin cancers,cutaneous squamous cell carcinoma(cSCC)is the most common one,which requires timely screening and better *** To identify risk factors and predict the incidence of cSCC for heart transplant *** We retrospectively analyzed adult heart transplant recipients between 2000 and 2015 extracted from the United Network for Organ Sharing *** whole dataset was randomly divided into a derivation set(80%)and a validation set(20%).Uni-and multivariate Cox regression were done to identify significant risk factors associated with the development of *** operating characteristics curves were generated and area under the curve(AUC)was calculated to assess the accuracy of the prediction *** on the selected risk factors,a risk scoring system was developed to stratify patients into different risk groups.A cumulative cSCC-free survival curve was generated using the Kaplan-Meier method for each group,and the log-rank test was done to compare the intergroup cSCC *** There were 23736 heart-transplant recipients during the study period,and 1827 of them have been reported with *** predictors of post-transplant cSCC were older age,male sex,white race,recipient and donor human leukocyte antigen(HLA)mismatch level,malignancy at listing,diagnosis with restrictive myopathy or hypertrophic myopathy,heart re-transplant,and induction therapy with OKT3 or *** multivariate model was used to predict the 5-,8-and 10-year incidence of cSCC and respectively provided AUC of 0.79,0.78 and 0.77 in the derivation set and 0.80,0.78 and 0.77 in the validation *** risk scoring system assigned each patient with a

关键词： Cutaneous squamous cell carcinoma Heart transplantation Cox proportional hazard model Risk assessment Squamous cell carcinoma Mortality outcomes