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Studies on Enterotoxins and Antimicrobial Resistance in Staphylococcus aureus Isolated from Various Sources

Advances in Microbiology 2016年第4期6卷 263-275页

作者： enas yasser mohammed Shaymaa Hassan Abdel-Rhman Rasha Barwa mohammed Adel El-Sokkary Microbiology and Immunology Department Faculty of Pharmacy Mansoura University Mansoura Egypt

Staphylococcus aureus represents a public health challenge all over the world. Therefore, this study aims to analyze the prevalence of five genes (sea, seb, sec, see and seg) encoding the staphylococcal enterotoxins in S. aureus isolated from different sources and to evaluate the association of these toxins in comparison to susceptibility towards 12 antimicrobials;antimicrobial susceptibility was conducted by disc diffusion method. Detection of staphylococcal enterotoxins was performed by PCR and the ability to express these genes was assessed among isolates by RT-PCR. The most common enterotoxin gene was sea gene (66%), followed by seb, sec, see and seg (38%, 23%, 19% and 5%) respectively. Expression of sea, seb and seg genes was variable. However, sec and see genes were not expressed by any of the tested isolates. No statistically significant association exists between (seb, sec and see) and isolation sources, while the sea was significantly associated with clinical isolates. High significant correlation was found between elevated sea expression and multidrug-resistance. Our findings indicate that the pathogenic potential of S. aureus may be greater than previously thought. This emphasizes the utmost need to implement proactive measures and more emphasis will be placed on the application of hygiene practices in hospitals to control S. aureus infection and enterotoxins production.

关键词： S. aureus Antimicrobial Susceptibility Enterotoxins Expression

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Use of the urinary trypsinogen-2 dipstick test in early diagnosis of pancreatitis after endoscopic retrograde cholangiopancreatography (ERCP)

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Open Journal of Gastroenterology 2013年第6期3卷 289-294页

作者： Hasan El-Garem enas Hamdy Sherif Hamdy Mohammad El-Sayed Aisha Elsharkawy Azmi mohammed Saleh Department of Clinical Pathology Faculty of Medicine Cairo University Cairo Egypt Department of Endemic Medicine and Hepatology Faculty of Medicine Cairo University Cairo Egypt

Background: Acute pancreatitis is one of the most serious complications of ERCP. Early diagnosis of post ERCP pancreatitis helps physicians to provide intensive care and possible medical treatment as early as possible. Trypsinogen-2 in urine is a good diagnostic and prognostic marker of acute pancreatitis. Objectives: To evaluate the diagnostic value of urinary trypsinogen-2 dipstick test for early diagnosis of post ERCP pancreatitis. Methods: A total of 37 patients with obstructive jaundice were tested with the urinary trypsinogen-2 dipstick test and serum levels of amylase and lipase before ERCP and 6 hours after ERCP. Results: Post ERCP pancreatitis was diagnosed in 6 (16%) of 37 patients. The sensitivity, specificity, positive predictive value and negative predictive value of urinary trypsinogen-2 dipstick test at 6 hours after ERCP were 100%, 97%, 86%, 100% respectively. At the cutoff level (130 U/L) for lipase, the positive predictive value and negative predictive value all were (100%), however, the positive predictive value and negative predictive value for amylase levels at cutoff (122 U/L) were 60%, 100% respectively. Serum lipase level was the best test for diagnosing post ERCP pancreatitis followed by the urinary trypsinogen-2 dipstick test. Conclusions: The urinary trypsinogen-2 dipstick test can be used as a rapid and easy test for early diagnosis of post ERCP pancreatitis with high sensitivity and specificity.

关键词： ERCP Pancreatitis Urinary Trypsinogen-2 Dipstick Test

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Amblyopia screening for first and second-grade children in Jordan

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International Journal of Ophthalmology(English edition) 2022年第2期15卷 352-356页

作者： Khalil M.Al-Salem Mohammad S.Saleem Ismat Ereifej Hamzeh M.Alrawashdeh Rayed Falah Hussein Obeidat Asma Ali Abdlmohdi Raeda Zaki Al-Momani Layal mohammed Hammad Omar Ahmad al-Habahbeh Yaqeen M.Assassfeh Laith Ramzi Najib Al-Qsous Ashraf Hatim Adeeb Nasraween Tariq A.Al Maaitah Deya'-Aldin Muneer Hasan Aqeel Ibtehal Eyadeh Awad Alabdulrazzg Abdullah Mahmoud Mohammad Murad Amany Jehad Faleh AL-Zurqan enas Jamal Mohammad Jaradat Slsabela Emad Suliman Aldhoon Ala'a Majed Dmour Ahmad Talal Asassfeh Jeries Ayman Halaseh Abdel Aziz Ammar Daoud Waha Ayman Alkasasbeh Kholoud GH.Matar Department of Ophthalmology and Visual Sciences Mutah UniversityAl-Karak 11183Jordan Royal Medical Services Department of Ophthalmology Amman 11855Jordan Department of Ophthalmology and Visual Sciences Al-Sharif Eye CenterAmman 11194Jordan Jordanian Menistrery of Health IrbidAmman 11855Jordan National Woman's Health Care Center(NWHCC) Amman 11855Jordan

AIM:To share the results of a national screening program for amblyopia in school children in the north of ***:This is a prospective national screening study for *** program rolls first and second-grade children(6 to 7 years old) in the north of *** eye examination included:best-corrected visual acuity,cover-uncover test,and cycloplegic *** visual acuity was tested using an ETDRS visual acuity chart without ***,children were tested with full cycloplegic refraction when the test criteria were *** amblyopia was defined as a best-corrected visual acuity difference of 2 or more *** comparison,bilateral amblyopia was defined as a best-corrected visual acuity of 20/40 or worse in the best ***:The prevalence of amblyopia for the total sample tested(n=17 203) was 2.78%(n=479).The most common cause of amblyopia was hypermetropia(64.45%),followed by previous ocular surgeries(15.1%),myopia(10.43%),strabismus(9.39%),and congenital cataract(0.63%).CONCLUSION:This is the first and only study,identifing modifiable risk factors in Jordanian children with *** their first couple of years of elementary education,many Jordanian children are affected by amblyopia and pass unnoticed.A more governmental effort is needed into screening programs to improve vision in the Jordanian population.

关键词： amblyopia screening Jordan community ophthalmology pediatric ophthalmology population-based study screening program

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