检索结果-南通市图书馆

A simple nanobody-based competitive ELISA to detect antibodies against African swine fever virus

Virologica Sinica 2022年第6期37卷 922-933页

作者： Jiakai Zhao Jiahong Zhu Ying Wang Mengting Yang Qiang Zhang Chong Zhang Yuchen Nan en-min zhou Yani Sun Qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F UniversityYangling Observing and Experimental Station of National Data Center of Animal HealthMinistry of AgricultureYangling712100China Kunming Customs Technology Center Kunming650228China

African swine fever virus(ASFV) infection is a big threat to the global pig industry. Because there is no effective vaccine, rapid, low-cost, and simple diagnosis methods are necessary to detect the ASFV infection in pig herds.Nanobodies, with advantages of small molecular weight and easy genetic engineering, have been universally used as reagents for developing diagnostic kits. In this study, the recombinant ASFV-p30 was expressed and served as an antigen to immunize the Bactrian camel. Then, seven nanobodies against ASFV-p30 were screened using phage display technique. Subsequently, the seven nanobodies fused horseradish peroxidase(nanobody-HRP) were secretory expressed and one fusion protein ASFV-p30-Nb75-HRP was selected with the highest sensitivity in blocking ELISA. Using the ASFV-p30-Nb75-HRP fusion protein as a probe, a competitive ELISA(cELISA) was developed for detecting anti-ASFV antibodies in pig sera. The cut-off value of cELISA was determined to be 22.7%by testing 360 negative pig sera. The detection limit of the cELISA for positive pig sera was 1:320, and there was no cross-reaction with anti-other swine virus antibodies. The comparative assay showed that the agreement of the cELISA with a commercial ELISA kit was 100%. More importantly, the developed cELISA showed low cost and easy production as a commercial kit candidate. Collectively, a simple nanobody-based cELISA for detecting antibodies against ASFV is developed and it provides a new method for monitoring ASFV infection in the pig herds.

关键词： African swine fever virus(ASFV) Nanobody ASFV-p30 Nanobody-HRP fusion Protein Competitive ELISA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparative genomic analysis of Thermus provides insights into the evolutionary history of an incomplete denitrification pathway

引用

mLife 2022年第2期1卷 198-209页

作者： Jian-Yu Jiao Zheng-Han Lian Meng-Meng Li Nimaichand Salam en-min zhou Lan Liu Hong ming Guoxing Nie Wensheng Shu Guoping Zhao Brian P.Hedlund Wen-Jun Li State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)School of Life SciencesSun Yat-Sen UniversityGuangzhouChina International Joint Research Center for Karstology School of Earth SciencesYunnan UniversityKunmingChina Synthetic Biology engineering Laboratory of Henan Province College of Life Sciences and TechnologyXinxiang Medical UniversityXinxiangChina College of Fisheries Henan Normal UniversityXinxiangChina Institute of Ecological Science School of Life ScienceSouth China Normal UniversityGuangzhouChina Institute of Synthetic Biology Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina School of Life Sciences University of NevadaLas VegasNevadaUSA Nevada Institute of Personalized Medicine University of NevadaLas VegasNevadaUSA State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqiChina

Biological denitrification is a crucial process in the nitrogen biogeochemical cycle,and Thermus has been reported to be a significant heterotrophic denitrifier in terrestrial geothermal environments.However,neither the denitrification potential nor the evolutionary history of denitrification genes in the genus Thermus or phylum Deinococcota is well understood.Here,we performed a comparative analysis of 23 Thermus genomes and identified denitrification genes in 15 Thermus strains.We confirmed that Thermus harbors an incomplete denitrification pathway as none of the strains contain the nosZ gene.Ancestral character state reconstructions and phylogenetic analyses showed that narG,nirS,and norB genes were acquired by the last common ancestor of Thermales and were inherited vertically.In contrast,nirK of Thermales was acquired via two distinct horizontal gene transfers from Proteobacteria to the genus Caldithermus and from an unknown donor to the common ancestor of all known Thermus species except Thermus filiformis.This study expands our understanding of the genomic potential for incomplete denitrification in Thermus,revealing a largely vertical evolutionary history of the denitrification pathway in the Thermaceae,and supporting the important role for Thermus as an important heterotrophic denitrifier in geothermal environments.

关键词： comparative genomics denitrification evolutionary history Thermus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Spatiotemporal regulation of ubiquitin-mediated protein degradation via upconversion optogenetic nanosystem

引用

Nano Research 2020年第12期13卷 3253-3260页

作者： Yafeng Hao Taofeng Du Gaoju Pang Jiahua Li Huizhuo Pan Yingying Zhang Lizhen Wang Jin Chang en-min zhou Hanjie Wang School of Life Sciences Tianjin UniversityTianjin300072China Department of preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F UniversityYangling712100China

Protein degradation technology,which is one of the most direct and effective ways to regulate the life activities of cells,is expected to be applied to the treatment of various diseases.However,current protein degradation technologies such as some small-molecule degraders which are unable to achieve spatiotemporal regulation,making them difficult to transform into clinical applications.In this article,an upconversion optogenetic nanosystem was designed to attain accurate regulation of protein degradation.This system worked via two interconnected parts:1)the host cell expressed light-sensitive protein that could trigger the ubiquitinproteasome pathway upon blue-light exposure;2)the light regulated light-sensitive protein by changing light conditions to achieve regulation of protein degradation.Experimental results based on model protein(Green Fluorescent Protein,GFP)validated that this system could fulfill protein degradation both in vitro(both Hela and 293T cells)and in vivo(by upconversion optogenetic nanosystem),and further demonstrated that we could reach spatiotemporal regulation by changing the illumination time(0–25 h)and the illumination frequency(the illuminating frequency of 0–30 s every 1 min).We further took another functional protein(The Nonstructural Protein 9,NSP9)into experiment.Results confirmed that the proliferation of porcine reproductive and respiratory syndrome virus(PRRSV)was inhibited by degrading the NSP9 in this light-induced system,and PRRSV proliferation was affected by different light conditions(illumination time varies from 0–24 h).We expected this system could provide new perspectives into spatiotemporal regulation of protein degradation and help realize the clinical application transformation for treating diseases of protein degradation technology.

关键词： protein degradation ubiquitin-proteasome system optogenetics upconversion materials the nonstructural protein9(NSP9) porcine reproductive and respiratory syndrome virus(PRRSV)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microbial dark matter coming to light: challenges and opportunities

引用

National Science Review 2021年第3期8卷 12-16页

作者： Jian-Yu Jiao Lan Liu Zheng-Shuang Hua Bao-Zhu Fang en-min zhou Nimaichand Salam Brian P.Hedlund Wen-Jun Li State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) School of Life Sciences Sun Yat-Sen University Department of environmental Science and engineering University of Science and Technology of China State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences School of Life Sciences University of Nevada Las Vegas Nevada Institute of Personalized Medicine University of Nevada Las Vegas

Microbes are the most abundant and diverse cellular life forms on Earth and colonize a wide range of environmental niches. However, more than 99%of bacterial and archaeal species have not been obtained in pure culture... 详细信息

关键词： BACTERIA

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Boost VS_(2) electrochemical reactive kinetics by regulating crystallographic planes and coupling carbon matrix for high performance sodium-ion storage

引用

Rare Metals 2024年第1期43卷 98-112页

作者： min Zeng ming-Shan Wang Lin Chen en-Zhi Li Zhen-Liang Yang Dan zhou Hai-Jiao Xie Jun-Chen Chen Zhi-Yuan Ma Bing-Shu Guo Bo Yu Xing Li School of New energy and Materials Southwest Petroleum UniversityChengdu 610500China energy Storage Research Institute Southwest Petroleum UniversityChengdu 610500China Institute of Materials China Academy of Engineering PhysicsMianyang 621908China Beijing Advanced Innovation Center for Materials Genome engineering University of Science and TechnologyBeijingBeijing 100083China Hangzhou Yanqu Information Technology Co. Ltd.Hangzhou 310003China

Vanadium disulfide(VS_(2)) as a typical two-dimensional transition metal chalcogenide has excellent competitiveness for sodium-ion storage due to its wide layer spacing(0.575 nm),high theoretical capacity of 932mAh·g^(-1) originating from multi-electron electrochemical redox.However,continuous sodiation process accompanied by crystal structural evolution and collapse cause rapid capacity decaying.Herein,novel few-layer VS_(2)nanosheets with open(001) crystal planes are in-situ constructed on reduced graphene oxide to solve these issues mentioned above.It indicates that few-layer VS_(2)provides more Na^(+) storage activity due to the low Na^(+)surface migration energy barrier on exposed crystal(001)planes.The flexible and high electronic conductivity of carbon matrix also effectively builds multi-level buffer structure and electron transport kinetics to boost the Na^(+)insertion/conversion reactive activity on VS_(2) as well as Na^(+) pseudocapacitance storage kinetics on edges and defects of nano sheets.Those coupling effects result in high rate capability and long cycling stability as a battery/capacitor anode.It delivers conspicuous high energy density of 81 and 40 Wh·kg^(-1) at power density of 118 and 10,286W·kg^(-1),as well as 80% energy retention rate after 5000cycles,confirming its great application potential in sodiumbased storage devices.

关键词： Vanadium disulfide Reduced graphene oxide Anode Sodium-ion battery Sodium-ion capacitor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Experimental and numerical simulation study on the erosion behavior of the elbow of gathering pipeline in shale gas field

引用

Petroleum Science 2024年第2期21卷 1257-1274页

作者： en-Bin Liu Shen Huang Ding-Chao Tian Lai-min Shi Shan-Bi Peng He Zheng Petroleum engineering School Southwest Petroleum UniversityChengdu 610500SichuanChina Chongqing Natural Gas Sales Center Natural Gas BranchSinopecChongqing400023China Zhejiang Zheneng Natural Gas Operation Co. Ltd.Hangzhou 310000ZhejiangChina School of Civil engineering and Architecture Southwest Petroleum UniversityChengdu 610500SichuanChina Louisiana Tech Univ Trenchless Technol Ctr599 Dan Reneau DrRustonLA71270USA Southwest Oil and Gas Field Branch Natural Gas Research Institute Chengdu 610213SichuanChina

During the production period of shale gas, proppant particles and rock debris are produced together,which will seriously erode the elbows of gathering pipelines. In response to this problem, this paper takes the elbow of the gathering pipeline in the Changning Shale Gas Field as an example to test the erosion rate and material removal mechanism of the test piece at different angles of the elbow through experiments and compares the four erosion models with the experimental results. Through analysis, it is found that the best prediction model for quartz sand-carbon steel erosion is the Oka model. Based on the Oka model, FLUenT software was used to simulate and analyze the law of erosion of the elbow of the gas gathering pipeline under different gas flow velocities, gas gathering pressure, particle size, length of L1,and bending directions of the elbow. And a spiral pipeline structure is proposed to reduce the erosion rate of the elbow under the same working conditions. The results show that this structure can reduce erosion by 34%.

关键词： Shale gas Gas gathering pipeline Elbow Erosion CFD

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Factors influencing willingness to participate in ophthalmic clinical trials and strategies for effective recruitment

引用

International Journal of Ophthalmology(english edition) 2024年第1期17卷 157-163页

作者： Jia-en Liang Meng-Yin Liang en-en Zhang Yu-Ying Peng Ling-Ling Chen Jia-Yu Deng Ting Lin Jun Fu Jia-Ni Zhang Shao-Li Li Fei Li Hui-ming Xaio Wen-min Huang Yu-Hong Liu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangdong Provincial Clinical Research Center for Ocular DiseasesGuangzhou 510060Guangdong ProvinceChina

AIM:To explore the factors influencing individuals’willingness to participate in ophthalmic clinical trials.METHODS:A questionnaire survey was conducted from January to April 2021 among patients and their family members at Zhongshan Ophthalmic Center,Sun Yat-sen University,in Guangzhou,China.The survey gathered data on respondents’willingness,demographic and socioeconomic profiles,as well as their reasons and concerns regarding engagement in clinical trials.RESULTS:Of the 1078 residents surveyed(mean age 31.2±13.1y;65.8%females)in Guangzhou,749(69.5%)expressed a willingness to participate in future ophthalmic clinical trials.Specific characteristics associated with greater willingness included a younger age,lower annual income,higher education,prior participation experience,previous ophthalmic treatment,and a better understanding of clinical trials.With the exception of age,these characteristics were significantly linked to a higher willingness.The primary barrier to participation,expressed by 64.8%of those willing and 54.4%of those unwilling,was“Uncertain efficacy”.In terms of motivations,the willing group ranked“Better therapeutic benefits”(35.0%),“Professional monitoring”(34.3%),and“Trust in healthcare professionals”(33.1%)as their top three reasons,whereas the unwilling participants indicated“Full comprehension of the protocol”(46.2%)as the key facilitator.CONCLUSION:This study reveals a substantial willingness to participate in ophthalmic clinical trials and demonstrates the predictive role of demographic and socioeconomic factors.Variations in motivators and concerns between willing and unwilling participants highlight the significance of tailored recruitment strategies.Importantly,the need for and trust in healthcare professionals stand out as powerful motivations,underscoring the importance of enhancing physician-patient relationships,adopting patient-centered communication approaches,and addressing individualized needs to improve accrual rates.

关键词： participation willingness recruitment strategy ophthalmic clinical trial

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation through induced apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway

Porcine reproductive and respiratory syndrome virus inhibits...

引用

第十三届全国免疫学学术大会

作者： Yang Mu Ximeng Han Cunyu Jia Xin Zhang Yunjie Jiao Taofeng Du en-min zhou Northwest A&F University

Background: Porcine reproductive and respiratory syndrome virus is an important immunosuppressive virus that primarily infects cells derived from the monocytic lineage, particularly differentiated macrophages, and strongly suppresses the proliferation of infected cells. Numerous studies have suggested that many viruses and their related proteins can perturb the cell cycle and induce cell apoptosis during infection. Although studied extensively, the effects of pathogenic mechanisms of PRRSV infection on the cell cycle and the corresponding molecular mechanism have not been reported.Results: In this work, we examined the ability of PRRSV to manipulate cell cycle progression in infected cells and explored the potential molecular mechanisms. The results showed that PRRSV infection imposed a growth-inhibitory effect on MARC-145 cells by inducing cell cycle arrest at the G2/M phase. This G2/M arrest was due to the significant decrease of Cdc2-cyclinB1 complex activity in PRRSV-infected MARC-145 cells. Cdc2-cyclinB1 complex activity reduction was due to Tyr15 phosphorylation of Cdc2 and accumulation in the nucleus of Cdc2 and CyclinB1. Not only elevated Wee1 and Myt1 expression and inactivated Cdc25 C, but also increased expression of the cell cycle regulatory molecule p21 and 14-3-3σ in a p53-dependent manner caused the inhibitory Tyr15 phosphorylation of Cdc2. In addition, PRRSV infection also activated the DNA damage checkpoint kinases, Chk1.Conclusions: Our data suggest that PRRSV infection induces G2/M arrest in infected MARC-145 cells via various molecular regulatory mechanisms. Our findings reveal the potential mechanisms involved in immune suppression and modulation mediated by PRRSV infection and provide new insights relevant to the pathogenesis of PRRSV.

关键词： Porcine reproductive and respiratory syndrome virus G2/M arrest Cdc2-cyclinB1 complex Cdc25C p53

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Downregulation of Serum PTen Expression in Mercury-Exposed Population and PI3K/AKT Pathway-Induced Inflammation

引用

Biomedical and environmental Sciences 2024年第4期37卷 354-366页

作者： MEI Peng DING en min YIN Hao Yang DING Xue Xue WANG Huan WANG Jian Feng HAN Lei ZHANG Heng Dong ZHU Bao Li School of Public Health Nanjing Medical UniversityNanjing 210000JiangsuChina Institute of Occupational Disease Prevention Jiangsu Province Center for Disease Prevention and ControlNanjing 21000JiangsuChina Suzhou Center for Disease Prevention and Control Suzhou 215004JiangsuChina Center for Global Health School of Public HealthNanjing Medical UniversityNanjing 210000JiangsuChina

Objective This study investigated the impact of occupational mercury(Hg) exposure on human gene transcription and expression, and its potential biological mechanisms.Methods Differentially expressed genes related to Hg exposure were identified and validated using gene expression microarray analysis and extended validation. Hg-exposed cell models and PTen lowexpression models were established in vitro using 293T cells. PTen gene expression was assessed using qRT-PCR, and Western blotting was used to measure PTen, AKT, and PI3K protein levels. IL-6 expression was determined by ELISA.Results Combined findings from gene expression microarray analysis, bioinformatics, and population expansion validation indicated significant downregulation of the PTen gene in the high-concentration Hg exposure group. In the Hg-exposed cell model(25 and 10 μmol/L), a significant decrease in PTen expression was observed, accompanied by a significant increase in PI3K, AKT, and IL-6 expression.Similarly, a low-expression cell model demonstrated that PTen gene knockdown led to a significant decrease in PTen protein expression and a substantial increase in PI3K, AKT, and IL-6 levels.Conclusion This is the first study to report that Hg exposure downregulates the PTen gene, activates the PI3K/AKT regulatory pathway, and increases the expression of inflammatory factors, ultimately resulting in kidney inflammation.

关键词： PTen Occupational mercury exposure Occupational health PI3K/AKT pathway 293T cell IL-6

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic mutation of Tas2r104/Tas2r105/Tas2r114 cluster leads to a loss of taste perception to denatonium benzoate and cucurbitacin B

引用

Animal Models and Experimental Medicine 2024年第3期7卷 324-336页

作者： Bowen Niu Lingling Liu Qian Gao Meng-min Zhu Lixiang Chen Xiu-Hua Peng Boying Qin Xiaohui zhou Feng Li Department of Laboratory Animal Science Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina Department of Biology College of Life SciencesShanghai Normal UniversityShanghaiPeople's Republic of China

Background:Bitter taste receptors(Tas2rs)are generally considered to sense various bitter compounds to escape the intake of toxic substances.Bitter taste receptors have been found to widely express in extraoral tissues and have important physiological functions outside the gustatory system in vivo.Methods:To investigate the physiological functions of the bitter taste receptor cluster Tas2r106/Tas2r104/Tas2r105/Tas2r114 in lingual and extraoral tissues,multiple Tas2rs mutant mice and Gnat3 were produced using CRISPR/Cas9 gene-editing technique.A mixture containing Cas9 and sgRNA mRNAs for Tas2rs and Gnat3 gene was microinjected into the cytoplasm of the zygotes.Then,T7en1 assays and sequencing were used to screen genetic mutation at the target sites in founder mice.Quantitative real-time polymerase chain reaction(qRT-PCR)and immunostaining were used to study the expression level of taste signaling cascade and bitter taste receptor in taste buds.Perception to taste substance was also studied using twobottle preference tests.Results:We successfully produced several Tas2rs and Gnat3 mutant mice using the CRISPR/Cas9 technique.Immunostaining results showed that the expression of GNAT3 and PLCB2 was not altered in Tas2rs mutant mice.But qRT-PCR results revealed the changed expression profile of m Tas2rs gene in taste buds of these mutant mice.With two-bottle preference tests,these mutant mice eliminate responses to cycloheximide due to genetic mutation of Tas2r105.In addition,these mutant mice showed a loss of taste perception to quinine dihydrochloride,denatonium benzoate,and cucurbitacin B(CuB).Gnat3-mediated taste receptor and its signal pathway contribute to CuB perception.Conclusions:These findings implied that these mutant mice would be a valuable means to understand the biological functions of TAS2Rs in extraoral tissues and investigate bitter compound-induced responses mediated by these TAS2Rs in many extraoral tissues.

关键词： bitter taste receptor CRISPR/Cas9 genetic mutation two-bottle preference test type 2 taste receptors(Tas2rs)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：