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Effect of preoperative transcatheter arterial chemoembolization on angiogenesis of hepatocellular carcinoma cells

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World Journal of Gastroenterology 2009年第36期15卷 4582-4586页

作者： en-hua xiao Dong Guo Du-lun Bian Department of Radiology the Second Xiangya Hospital Central SouthUniversity Changsha 410011 Htman Province China

AIM： To evaluate the effects of four types of preoperative transcatheter arterial chemoembolization （TACE） on angiogenesis of hepatocellular carcinoma （HCC） ceils. METHODS： A total of 136 patients with HCC underwent liver resection. One to five courses of TACE prior to liver resection were performed in 79 patients （TACE group）, in which one to four courses of chemotherapy alone were performed in 11 patients （group A）; one to five courses of chemotherapy combined with iodized oil were performed in 33 patients （group B）; one to three courses of chemotherapy combined with iodized oil and gelatin sponge were performed in 23 patients （group C）, one to three courses of chemotherapy combined with iodized oil, ethanol and gelatin sponge were performed in 12 patients （group D）. The other 57 patients only received liver resection （non-TACE group）. The microvessels were marked by CD31. The expression of CD31 and vascular endothelial growth factor （VEGF） protein were detected by immunohistochemical methods. RESULTS： The mean microvessel density （MVD） in HCC cells was significantly higher in groups A, B, C and D than in the nonACE group （P 〈 0.05）. The expression of VEGF protein in HCC cells were significantly higher in groups A, B, C and D than in the non-TACE group （P 〈 0.05）. MVD and the expression of VEGF protein were positively correlated. Mean MVD and the expression of VEGF protein were closely related to the number of courses of TACE and the interval of TACE. CONCLUSION： Four different types of preoperative TACE regimens enhanced angiogenesis in HCC cells by up-regulating the expression of VEGF protein. It is necessary to repress angiogenesis of liver cancer after TACE.

关键词： Angiogenesis Hepatocellular carcinoma Immunohistochemistry Transcatheter arterial chemoembolization Vascular endothelial growth factor

Effect of preoperative transcatheter arterial chemoembolization on proliferation of hepatocellular carcinoma cells

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World Journal of Gastroenterology 2007年第33期13卷 4509-4513页

作者： en-hua xiao Jing-Qing Li Jie-Fu huang Department of Radiology the Second Xiangya Hospital Central South University Changsha 410011 Hunan Province China Liver Cancer Research Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 Guangdong

AIM： To evaluate the effect of preoperative transcatheter arterial chemoembolization （TACE） on proliferation of hepatocellular carcinoma （HCC） cells. METHODS： A total of 136 patients with HCC underwent liver resection. Of 136 patients, 79 patients received 1 to 5 courses of TACE prior to liver resection （TACE group）, who were further subdivided into four groups： Group A （n = 11） who received i to 4 courses of chemotherapy alone; Group B （n = 33） who received i to 5 courses of chemotherapy combined with iodized oil; Group C （n = 23） who received i to 3 courses of chemotherapy combined with iodized oil and gelatin sponge; and Group D （n = 12） who received 1 to 3 courses of chemotherapy combined with iodized oil, ethanol and gelatin sponge. The other 57 patients only received liver resection （non- TACE group）. The expressions of Ki-67 and proliferating cell nuclear antigen （PCNA） protein were detected in the liver cancer tissues by immunohistochemical method. RESULTS： The Ki-67 protein expression was significantly lower in Groups C and D as compared with non-TACE group （31.35% ± 10.85% vs 44.43% ± 20.70%, 30.93% ± 18.10% vs 44.43% ± 20.70%, respectively, P 〈 0.05）. The PCNA protein expression was significantly lower in Groups C and D as compared with non-TACE group （49.61% ± 15.11% vs 62.92% ± 17.21%, 41.16% ± 11.83% vs 62.92% ± 17.21%, respectively, P 〈 0.05）. The Ki-67 protein expression was significantly higher in Group A as compared with non-TACE group （55.44% ± 13.72% vs 44.43% ± 20.70%, P 〈 0.05）. The PCNA protein expression was significantly higher in Groups A and B as compared with non-TACE group （72.22% ± 8.71% vs 62.92% ± 17.21%, 69.91% ± 13.38% vs 62.92% ± 17.21%, respectively, P 〈0.05）. CONCLUSION： Preoperative multi-material TACE suppresses the proliferation of HCC cells, while a single material embolization and chemotherapy alone enhance the proliferation of HCC cells.

关键词： Hepatocellular carcinoma Transcatheter arterial chemoembolization Proliferating cell nuclear antigen Ki-67

Gene expression and MR diffusion-weighted imaging after chemoembolization in rabbit liver VX-2 tumor model

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World Journal of Gastroenterology 2008年第36期14卷 5557-5563页

作者： You-Hong Yuan en-hua xiao Jian-Bin Liu Zhong He Ke Jin Cong Ma Jun Xiang Jian-hua xiao Wei-Jian Chen Department of Radiology Hunan Province People's Hospital Changsha 410005 HtmanProvince China Departmentof Radiology the Second Xiang-Ya Hospital Central SouthUniversity Changsha 410005 Hunan Province China Department of Pathology HunanProvince Children's Hospital Changsha 410005 HunanProvince China Department of Epidemiology Center forDisease Control of Hunan Province Changsha 410005 HunanProvince China

AIM: To investigate the dynamic characteristics and the correlation between PCNA, Bax, nm23, E-cadherin expression and apparent diffusion coefficient (ADC) on MR diffusion-weighted imaging (DWI) after chemoembolization in rabbit liver VX-2 tumor model. METHODS: Forty New Zealand rabbit liver VX-2 tumor models were included in the study. DWI was carried out periodically after chemoembolization. All VX-2 tumor samples in each group were examined by histopathology and Strept Avidin-Biotin Complex (SABC) immunohistochemical staining. RESULTS: The PCNA expression index in VX-2 tumors was higher than in the normal parenchyma around the tumor (P enchyma around the tumor (all P en decreased (P en b-value was 100 s/mm2, there was a linear correlation between PCNA expression and ADC in the area of VX-2 tumor periphery (P enced the ADC. CONCLUSION: The potential of VX-2 tumor infiltrating and metastasizing decreases, while its ability to proliferate increases for a short time after chemoembolization. To some degree, the ADC value indirectly reflects the proliferation of VX-2 tumor cells.

关键词： Rabbit liver VX-2 tumor Chemoembolization Diffusion-weighted imaging Gene expression

Characteristics and pathological mechanism on magnetic resonance diffusion-weighted imaging after chemoembolization in rabbit liver VX-2 tumor model

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World Journal of Gastroenterology 2007年第43期13卷 5699-5706页

作者： You-Hong Yuan en-hua xiao Jian-Bin Liu Zhong He Ke Jin Cong Ma Jun Xiang Jian-hua xiao Wei-Jian Chen Department of Radiology HunanProvince People's Hospital Changsha 410005 Hunan ProvinceChina Departmentof Radiology the Second Xiang-Ya Hospital Central SouthUniversity Changsha 410005 Hunan Province China Department of Pathology HunanProvince Children's Hospital Changsha 410005 Hunan ProvinceChina Department of Epidemiology Center for DiseaseControl of Hunan Province Changsha 410005 Hunan ProvinceChina

AIM: To investigate dynamic characteristics and pathological mechanism of signal in rabbit VX-2 tumor model on diffusion-weighted imaging (DWI) after chemoembolization. METHODS: Forty New Zealand rabbits were included in the study and forty-seven rabbit VX-2 tumor models were raised by implanting directly and intrahepatically after abdominal cavity opened. Forty VX-2 tumor models from them were divided into four groups. DWI was performed periodically and respectively for each group after chemoembolization. All VX-2 tumor samples of each group were studied by pathology. The distinction of VX-2 tumors on DWI was assessed by their apparent diffusion coefficient (ADC) values. The statistical significance between different time groups, different area groups or different b-value groups was calculated by using SPSS12.0 software. RESULTS: Under b-value of 100 s/mm2, ADC values were lowest at 16 h after chemoembolization in area of VX-2 tumor periphery, central, and normal liver parenchyma around tumor, but turned to increase with further elongation of chemoembolization treatment. The distinction of ADC between different time groups was significant respectively (F = 7.325, P enchyma around tumor, increased quickly in sixteen h after chemoembolization but, from the 16th h to the 48th h, cellular edema in the area of normal liver parenchyma around tumor decreased gradually and that in the area of VX-2 tumor periphery decreased lightly at, and then increased continually. After chemoembolization, Cellular necrosis in the area of VX-2 tumor periphery was more significantly high than that before chemoembolization. The areas of dead cells in VX-2 tumors manifested low signal and high ADC value, while the areas of viable cells manifested high signal and low ADC value. CONCLUSION: DWI is able to detect and differentiate tumor necrotic areas from viable cellular

关键词： Liver VX-2 tumor Diffusion-weighted imaging Apparent diffusion coefficient Chemoembolization

Characteristics of liver on magnetic resonance diffusion-weighted imaging:Dynamic and image pathological investigation in rabbit liver VX-2 tumor model

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World Journal of Gastroenterology 2008年第25期14卷 3997-4004页

作者： You-Hong Yuan en-hua xiao Jian-Bin Liu Zhong He Ke Jin Cong Ma Jun Xiang Jian-hua xiao Wei-Jian Chen Department of Radiology Hunan Province People's Hospital Changsha 410005 HunanProvince China Departmentof Radiology the Second Xiangya Hospital Central SouthUniversity Changsha 410005 Hunan Province China Department of Pathology HunanProvince Children's Hospital Changsha 410005 HunanProvince China Department of Epidemiology Center forDisease Control of Hunan Province Changsha 410005 HunanProvince China

AIM： To investigate dynamical and image pathological characteristics of the liver on magnetic resonance （MR） diffusion-weighted imaging （DWI） in the rabbit VX-2 tumor model. METHODS： Forty New Zealand rabbits were included in the study and VX-2 tumor piece was implanted intrahepatically. Fifteen animals received two intrahepatic implantations while 25 had one intrahepatical implantation. DWI, T1- and T2-weighted of magnetic resonance imaging （MRI） were carried out on the 7th and the 14th d after implantation and DWI was conducted, respectively on the 21th d. Ten VX-2 tumor samples were studied pathologically. RESULTS： The rate of lump detected by DWI, TlWI and T2WI was 78.7%, 10.7% and 53.5% （X^2 =32.61, P 〈 0.001） on the 7th d after implantation and 95.8%, 54.3% and 82.9% （X^2 = 21.50, P 〈 0.001） on the 14th d. The signal of most VX-2 tumors on DWI was uniform and it was equal diffusion coefficient （ADC）. did not decrease on the 7th on the map of apparent The signal of VX tumors d after implantation, most of them slowly growing during the week following implantation without significant cell dying within the tumor. VX-2 tumors grew increasingly within 14 d after implantation but the signal of most VX-2 tumors on DWI or on the map of ADC was uniform or uneven and ADC of VX tumors decreased obscurely or slightly because tumor necrosis was still not obvious. On the 21th d after implantation, the signal of most VX-2 tumors on DWI or on the map of ADC was uneven because tumor necrosis was evident and ADC of VX-2 tumor necrotic areas decreased. The areas of viable cells in VX-2 tumors manifested a high signal on DWI and a low signal on the map of ADC. The areas of dead cells or necrosis in VX-2 tumors manifested low signals on DWI and low, equal or high signals on the map of ADC but they manifested high signals on DWI and on the map of ADC at the same time when the areas of necrotic tumor became liquefied or cystic. The border of tumors on DWI appeared gradually disti

关键词： Liver VX-2 tumor Diffusion-weightedimaging Apparent Diffusion Coefficient Rabbit

Pathological and MR-DWI study of the acute hepatic injury model after stem cell transplantation

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World Journal of Gastroenterology 2011年第23期17卷 2821-2828页

作者： Quan-Liang Shang en-hua xiao Qi-Chang Zhou Jian-Guang Luo Hai-Jun Wu Department of Radiology Second Xiangya HospitalCentral South University Changsha 410011 Hunan ProvinceChina Department of Ultrasound Second XiangyaHospital Central South University Changsha 410011 HunanProvince China

AIM： To investigate apparent diffusion coefficient （ADC） values as an indication of reconditioning of acute hepatic injury （AHI） after allogeneic mononuclear bone marrow cell （MBMC） transplantation. METHODS： Three groups were used in our study： a cell transplantation group （n = 21）, transplantation control group （n = 21） and normal control group （n = 10）. AHI model rabbits in the cell transplantation group were injected with 5 mL of MBMC suspension at multiple sites in the liver and the transplantation controls were injected with 5 mL D-Hanks solution. At the end of the 1st, 2nd and 4th wk, 7 rabbits were randomly selected from the cell transplantation group and trans- plantation control group for magnetic resonance diffu- sion-weighted imaging （MR-DWI） and measurement of the mean ADC values of injured livers. After MR-DWI examination, the rabbits were sacrificed and the livers subjected to pathological examination. Ten healthy rab- bits from the normal control group were used for MR- DWI examination and measurement of the mean ADC value of normal liver. RESULTS： At all time points, the liver pathological scores from the cell transplantation group were significantly lower than those in the transplantation control group （27.14± 1.46 vs 69.29± 6.16, 22.29 ± 2.29 vs 57.00± 1.53, 19.00 ± 2.31 vs 51.86 ± 6.04, P = 0.000）. The mean ADC values of the cell transplantation group were significantly higher than the transplantation con- trol group （（1.07± 0.07） ×10^-3 mm2/s vs （0.69 ± 0.05） ×10^-3 mm2/s, （1.41± 0.04） ×10^-3 mm2/s vs （0.84± 0.03） ×10^-3 mm2/s, （1.68 ± 0.04） ×10^-3 mm2/s vs （0.86± 0.04） ×10^-3 mm2/s, P = 0.000）. The pathological scores of the cell transplantation group and transplantation control group gradually decreased. However, their mean ADC values gradually increased to near that of the normal control. At the end of the 1st wk, the mean ADC values of the cell transplantation group and transplantation control group were significantly lower than those of the

关键词： Stem cells Transplantation Hepatic injury Magnetic resonance imaging Diffusion weighted imaging

Diagnostic value of gadobenate dimeglumine-enhanced hepatocyte-phase magnetic resonance imaging in evaluating hepatic fibrosis and hepatitis

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World Journal of Gastroenterology 2017年第17期23卷 3133-3141页

作者： Xiu-Mei Li Zhu Chen en-hua xiao Quan-Liang Shang Cong Ma Department of Radiology The Second Xiangya Hospital of Central South University Department of Radiology The First Affiliate Hospital of Fujian Medical University

AIM To evaluate the diagnostic value of gadobenate dimeglumine(Gd-BOPTA)-enhanced hepatocyte-phase magnetic resonance imaging(MRI) in evaluating hepatic fibrosis and *** Hepatocyte-phase images of Gd-BOPTA-enhanced MRI were retrospectively evaluated in 76 patients with chronic liver disease. These patients were classified into five groups according to either the histopathological fibrosis stage(S0-S4) or the histopathological hepatitis grade(G0-G4). The relative enhancement ratio(RE) of the liver parenchyma in the T1-vibe sequence was calculated by measuring the signal intensity before(SI pre) and 90 min after(SI post) intravenous injection of Gd-BOPTA using the following formula: RE =(SI post-SI pre)/SI pre. One-way analysis of variance was used to compare the difference between the relative RE in the hepatocyte phase(REh) and the stage of hepatic fibrosis and the grade of hepatitis. Pearson's productmoment correlation analysis was used to evaluate the relationship between the REh and the levels of serologic liver functional *** According to histopathological hepatic fibrosis stage, the 76 patients were classified into five groups: 16 in S0, 15 in S1, 21 in S2, 9 in S3, and 15 in S4 group. According to histopathological hepatitis grade, the 76 patients were also classified into five groups: 0 in G0, 44 in G1, 22 in G2, 8 in G3, and 2 in G3 group. With regard to the stage of hepatic fibrosis, REh showed significant differences between the S2 and S3 groups and between the S2 and S4 groups(P ence was observed between the other groups. With regard to the grade of hepatitis, REh showed significant differences between the G1 and G2 groups and between the G1 and G4 groups(P ence was observed between the other groups. Increased REh showed correlations with decreased serum levels of TB, ALT and AST(P ent, measuring the REh using Gd-BOPTAenhanced MR

关键词： Gd-BOPTA Magnetic resonance imaging Hepatocyte phase Relative enhancement Hepatic fibrosis

MR diffusion-weighed imaging of rabbit liver

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World Journal of Gastroenterology 2005年第35期11卷 5506-5511页

作者： You-Hong Yuan en-hua xiao Zhong He Jun Xiang Ke-Li Tang Rong-hua Yan Ke Jin Zi-Wen Peng Departmentof Radiology the Second Xiangya Hospital Central South University Changsha410011 Hunan Province China

AIM： To study the techniques of MR diffusion-weighed imaging （DWI） for normal rabbit liver. METHODS： After 15 normal New Zealand white rabbits and one New Zealand white rabbit implanted with VX-2 tumor were anesthetized with 30 soluble pentobarbitone, DWI was performed respectively for different b values, repetition times （TR） or thicknesses, when other parameters were the same and magnetic resonance imaging （MRI） was performed respectively, or with different field of views （FOV） or coil when other parameters were the same. The distinction between groups was analyzed by SPSS10.0 with apparent diffusion coefficient （ADC）, quality index （QI） or signal-noise ratio （SNR）. RESULTS： As b value increased, liver ADC, QI and SNR of DWI became smaller and simultaneously （F= 292.87, 156.1, 88.23, P〈0.01）. QI of DWI was high, when bvalue was 10, 50 or 100 respectively, but the distinction between them was insignificant; when b value was 800, QI and SNR of DWI were low. QI and SNR of DWI had no significant difference between TR = 4 000, 6 000 and 8 000. QI of DWI with 2 mm thickness was bigger than that with 5 mm thickness （t = 3.04, P〈0.01）, but SNR of DWI with 2 mm thickness was significantly smaller （t = -17.86, P〈0.01）. SNR of MRI with knee joint coil was obviously bigger than that with cranium coil It = -5.77 （TlWI） or -4.02 （T2WI）, P〈0.01], but QI of MRI was smaller on the contrary It = 7.10 （TlWI） or 3.97 （T2WI）, P〈0.01]. When FOV was enlarged gradually, SNR of MRI increased IF= 85.81 （T1WI） or 221.96 （T2WI）, P〈0.01], but QI firstly increased, then decreased IF= 68.67 （TlWI） or 69.46 （T2WI）, P〈0.01] and QI of MRI was the biggest when FOV was 20 crux15 cm. CONCLUSION： The scanning technique is very important in DWl of rabbit liver and the overall quality of DWl with b （100 s/mm2）, thickness （2 mm）, cranium coils and FOV（20 cm×15 cm） was best in our study, when other parameters were the same.

关键词： Liver Rabbits Magnetic resonance imaging Diffusion-weighted imaging Technology

Potential applications of single-incision laparoscopic totally preperitoneal hernioplasty

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World Journal of Gastrointestinal Surgery 2024年第7期16卷 2202-2210页

作者： xiao-Jun Wang Ting Fei Xiong-hua Xiang Quan Wang en-Cheng Zhou Department of Gastrointestinal Surgery The First Affiliated Hospital of Ningbo UniversityNingbo 315020Zhejiang ProvinceChina Ambulatory Surgery Center Xijing HospitalAir Force Military Medical UniversityXi’an 710032Shaanxi ProvinceChina

BACKGROUND The totally preperitoneal(TPP)approach is a new concept that was recently *** the TPP approach combined with single-incision laparoscopic hernia repair has its own advantages,there is little evidence reflecting the characteristics and feasibility of either *** To analyze the potential applications of single-incision laparoscopic TPP(SILTPP)inguinal hernia hernioplasty for the treatment of inguinal *** A total of 152 SIL-TPP surgeries were performed at the First Affiliated Hospital of Ningbo University from February 2019 to November 2022.A single-port,named Iconport,and standard laparoscopic instruments were used during the *** data,intraoperative parameters and short-term postoperative outcomes were collected and retrospectively *** The demographic data of 152 patients underwent SIL-TPP were shown in Table *** average age was 49.5 years(range from 21 to 81 years).The average body mass index was 27.7 kg/m^(2)(range from 17.7 kg/m^(2) to 35.6 kg/m^(2)).SIL-TPP were conducted successfully in 147 *** patients were converted to the SILtransabdominal preperitoneal laparoscopic herniorrhaphy at the initial stage of the study due to a lack of *** 2 patients with incisional hernias,an auxiliary operation hole was added during the SIL-TPP procedure,as required for *** mean operative time was 64.5 minutes(range:36.0-110.0 minutes)for unilateral direct and femoral hernias and 81.6 minutes for indirect hernias(range:40.0-150.0 minutes).The mean postoperative hospital stay was 3.4 *** SIL-TPP is feasible and has advantages for inguinal hernia ***-TPP has potential benefits for patients with various abdominal wall ***,doctors should be encouraged to actively apply the TPP approach combined with a single incision in their daily work.

关键词： Totally preperitoneal hernioplasty Iconport Single incision laparoscopic Totally extraperitoneal herniorrhaphy Single incision laparoscopic totally preperitoneal hernioplasty