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Neural Regeneration Research 2024年第10期19卷 2240-2248页

作者： Meng Yuan Yao Tang Tianwen huang Lining Ke en huang Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Human Anatomy School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Scientific Research Center School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology Fujian Medical University Union HospitalFuzhouFujian ProvinceChina Fujian Key Laboratory of Vascular Aging Fujian Medical UniversityFuzhouFujian ProvinceChina

In situ direct reprogramming technology can directly convert endogenous glial cells into functional neurons in vivo for central nervous system repair. Polypyrimidine tract-binding protein 1(PTB) knockdown has been shown to reprogram astrocytes to functional neurons in situ. In this study, we used AAV-PHP.e B-GFAP-sh PTB to knockdown PTB in a mouse model of ischemic stroke induced by endothelin-1, and investigated the effects of GFAP-sh PTB-mediated direct reprogramming to neurons. Our results showed that in the mouse model of ischemic stroke, PTB knockdown effectively reprogrammed GFAP-positive cells to neurons in ischemic foci, restored neural tissue structure, reduced inflammatory response, and improved behavioral function. These findings validate the effectiveness of in situ transdifferentiation of astrocytes, and suggest that the approach may be a promising strategy for stroke treatment.

关键词： astrocyte in situ direct reprogramming ischemic stroke miR-30 based shRNA neuron polypyrimidine tract-binding protein 1 transdifferentiation

Copper-catalyzed enantioselective desymmetrizing C（sp）–H functionalization of azide-ynamides via α-imino copper carbenes

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Science China Chemistry 2024年

作者： en-He huang Li-Gao Liu You-Wei Yin Hao-Xuan Dong Ji-Jia Zhou Xin Lu Bo Zhou Long-Wu Ye Key Laboratory for Chemical Biology of Fujian Province and State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical Engineering Xiamen University State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences

α-Imino metal carbenes are versatile intermediates in organic synthesis, and have broad applications in the assembly of divergent N-heterocycles. However, the catalytic enantioselective desymmetrization based on α-imino metal carbenes has not been developed to date. Herein, we disclose an enantioselective desymmetrizing C（sp2）–H functionalization of azide-ynamides via α-imino copper carbenes, leading to the efficient assembly of divergent chiral indoloazepines in generally moderate to excellent yields with high enantioselectivities. Notably, this reaction represents the first enantioselective desymmetrization based on α-imino metal carbenes. Further synthetic transformations and biological tests show the potential utility of this method. Moreover,computational studies are employed to elucidate the reaction mechanism and the origin of enantioselectivity.

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长链非编码RNA参与尿道下裂雄鼠雄激素的调控

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吉林医学 2024年第6期45卷 1275-1279页

作者：冯笑黄恩馥周云苏州大学实验动物中心江苏苏州215000 苏州大学附属儿童医院泌尿外科

目的:探讨雄性大鼠生殖结节中长链非编码RNA(LncRNA)在邻苯二甲酸二丁酯(DBP)诱导雄性大鼠尿道下裂形成中对雄激素调控的影响。方法:在孕鼠孕13~18 d时按体重800 mg/kg DBP溶于1 ml玉米油灌胃,对照组给予玉米油1 ml/d。于孕19 d时取出胎... 详细信息

目的:探讨雄性大鼠生殖结节中长链非编码RNA(LncRNA)在邻苯二甲酸二丁酯(DBP)诱导雄性大鼠尿道下裂形成中对雄激素调控的影响。方法:在孕鼠孕13~18 d时按体重800 mg/kg DBP溶于1 ml玉米油灌胃,对照组给予玉米油1 ml/d。于孕19 d时取出胎鼠,分别取下两组雄性胎鼠的生殖结节,进行全转录组基因测序。对测序结果中LncRNA进行筛选、差异分析和功能预测,再筛选8条LncRNA用qRT-PCR验证其表达量。结果:与对照组比较,尿道下裂组生殖结节中有598个差异mRNA,427个差异LncRNA,8条LncRNA差异趋势与测序结果一致,不存在差异趋势与测序结果的差异趋势相反的LncRNA。RT-PCR结果与测序结果一致。共筛选出与雄激素相关的差异表达LncRNAs 130个,与雄激素生物合成途径相关11个,与雄激素受体信号通路的负调控相关25个,与雄激素结合相关10个,与雄激素受体信号通路激活前列腺诱导相关29个,与雄激素代谢过程相关23个,与雄激素受体结合相关16个,与雄激素受体信号通路的正调控相关3个。结论:DBP诱导的尿道下裂雄性大鼠与正常雄性大鼠比较,其生殖结节中LncRNA可通过调控雄激素的合成、代谢、结合,雄激素受体的结合,雄激素受体信号通路这几个方面参与尿道下裂的形成。

关键词：邻苯二甲酸二丁酯尿道下裂长链非编码RNA 雄激素

Comprehensive analysis reveals hub genes associated with immune cell infiltration in allergic rhinitis

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2023年第4期9卷 340-351页

作者： Hui Li Shi-en huang Cong-Li Geng Yu-Xiao Wu Mu-Han Shi Min Wang Department of Otorhinolaryngology Peking University People's HospitalBeijingChina Department of Rhinology The First Affliated Hospital of Zhengzhou UniversityZhengzhouChina

Objectives:Allergic rhinitis(AR)refers to a form of respiratory inflammation that mainly affects the sinonasal mucosa.The purpose of this study was to explore the level of immune cell infiltration and the pathogenesis of AR.Methods:We performed a comprehensive analysis of two gene expression profiles(GSE50223 and GSE50101,a total of 30 patients with AR and 31 healthy controls).CIBERSORT was used to evaluate the immune cell infiltration levels.Weighted gene coexpression network analysis was applied to explore potential genes or gene modules related to immune status,and enrichment analyses including gene ontology,Kyoto encyclopedia of Genes and Genomes,gene set enrichment analysis,and gene set variation analysis,were performed to analyze the potential mechanisms in AR.A protein–protein interaction network was constructed to investigate the hub genes,and consensus clustering was conducted to identify the molecular subtypes of AR.Results:Compared to the healthy controls,patients with AR had high abundance levels and proportions of CD4+memory‐activated T cells.One hundred and eight immune‐related differentially expressed genes were identified.enrichment analysis suggested that AR was mainly related to leukocyte cell‐cell adhesion,cytokine‐cytokine receptor interaction,T‐cell activation,and T‐cell receptor signaling pathway.Ten hub genes,includingTYROBP,CSF1R,TLR8,FCER1G,SPI1,ITGAM,CYBB,FCGR2A,CCR1,andHCK,which were related to immune response,might be crucial to the pathogenesis of AR.Three molecular subtypes with significantly different immune statuses were identified.Conclusion:This study improves our understanding of the molecular mechanisms in AR via comprehensive strategies and provides potential diagnostic biomarkers and therapeutic targets of AR.

关键词： allergic rhinitis CIBERSORT consensus cluster GSEA GSVA immune cell infiltration WGCNA

Superconducting gap evolution of kagome metal CsV3Sb5 under pressure

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Science China(Physics,Mechanics & Astronomy) 2023年第2期66卷 109-114页

作者： Dongting Zhang Chufan Chen Lichang Yin Yan'en huang Fengrui Shi Yi Liu Xiaofeng Xu Huiqiu Yuan Xin Lu Center for Correlated Matter and Department of Physics Zhejiang UniversityHangzhou 310058China Key Laboratory of Quantum Precision Measurement of Zhejiang Province Department of Applied PhysicsZhejiang University of TechnologyHangzhou310023China Zhejiang Province Key Laboratory of Quantum Technology and Device Department of PhysicsZhejiang UniversityHangzhou 310058China StateKey Laboratory of Silicon Materials Zhejiang UniversityHangzhou 310058China Collaborative Innovation Center of Advanced Microstructures Nanjing 210093China

Kagome superconductors AV3Sb5(A=Cs,V,Sb) have served as an ideal platform to unveil the intriguing competitions between charge-density-wave(CDW),superconductivity and possible nontrivial topological states.We report soft point-contact spectroscopy(SPCS) studies on CsV3Sb5under pressure up to 2.5 GPa.Its superconducting transition temperature Tcand gaps for SPCS at pressure P=0.1 GPa suggest the absence of the adverse local strain effect as observed in point-contacts at ambient pressure,while the SPCS-determined Tcunder hydrostatic pressure deviates from the reported double dome feature below 2.5 GPa.Three distinct gap values in CsV3Sb5are proposed to exist through the whole pressure range with 2Δ1/kBTc≤1,2Δ2/kBTc~2.3-3.0 and 2Δ3/kBTc>3.5.The two smaller gaps for 2Δ1/kBTc≤1 and 2Δ2/kBTc~2.3-3.0 are robust under pressure,while there is no trace of the largest gap 2Δ3/kBTc>3.5 for SPCS in the c direction with P≤P1=0.7 GPa and P≥ P2=2.0 GPa and it becomes observable in the pressure range of(P1,P2),suggesting a complex relation between CDW and superconductivity between(P1,P2).

关键词： evolution hydrostatic superconductivity

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蚊虫复合组DNA芯片鉴定技术的初步研究

中国口岸科学技术 2024年第6期6卷 86-95页

作者：林诗颖彭晓丽曾志昌黄恩炯福州国际旅行卫生保健中心福建医科大学公共卫生学院榕城海关综合技术服务中心

本研究以ITS2基因为分子标记设计杂交探针，制作DNA芯片鉴定杂鳞库蚊复合组、白纹伊蚊亚组和尖音库蚊复合组中的8种/亚种蚊虫。结果表明，以ITS2基因为分子标记设计的DNA芯片可用于3个复合组（亚组）组间蚊虫以及杂鳞库蚊复合组的组内... 详细信息

本研究以ITS2基因为分子标记设计杂交探针，制作DNA芯片鉴定杂鳞库蚊复合组、白纹伊蚊亚组和尖音库蚊复合组中的8种/亚种蚊虫。结果表明，以ITS2基因为分子标记设计的DNA芯片可用于3个复合组（亚组）组间蚊虫以及杂鳞库蚊复合组的组内蚊虫鉴定，且白纹伊蚊亚组探针、尖音库蚊复合组探针、杂鳞库蚊复合组探针、三带喙库蚊探针、伪杂鳞库蚊探针、致倦库蚊探针、尖音库蚊探针具有良好的专一性和特异性。现场测试表明，该DNA芯片可应用于识别不同发育阶段和不同地区的同种蚊虫，但对尖音库蚊和致倦库蚊表现出非专一性杂合。

关键词：蚊虫复合组(亚组) DNA芯片鉴定

Risk of critical limb ischemia in long-term uterine cancer survivors:A population-based study

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World Journal of Clinical Cases 2022年第36期10卷 13293-13303页

作者： Min-Chi Chen Jung-Jung Chang Miao-Fen Chen Ting-Yao Wang Cih-en huang Kuan-Der Lee Chao-Yu Chen Department of Public Health Chang Gung UniversityTaoyuan 333Taiwan Department of Obstetrics and Gynecology Chang Gung Memorial HospitalChiayi 612Taiwan Division of Cardiovascular Disease Department of Internal MedicineChang Gung Memorial HospitalChiayi 612Taiwan Department of Radiation Oncology Change Gung Memorial HospitalLinkouTaoyuan 333Taiwan Division of Hematology and Oncology Department of Internal MedicineChang Gung Memorial HospitalChiayi 612Taiwan Graduate Institute of Clinical Medical Sciences College of MedicineChang Gung UniversityTaoyuan 333Taiwan Department of Medicine Taipei Medical University HospitalTaipei 100Taiwan Department of Medical Research Taichung Veterans General HospitalTaichung 407Taiwan Department of Post-Baccalaureate Medicine College of MedicineNational Chung Hsing UniversityTaichung 402Taiwan Department of Early Childhood Care and Education Shu-Zen Junior College of Medicine and ManagementKaohsiung 821Taiwan

BACKGROUND The risk of critical limb ischemia(CLI)which causes ischemic pain or ischemic loss in the arteries of the lower extremities in long-term uterine cancer(UC)survivors remains unclear,especially in Asian patients,who are younger at the diagnosis of UC than their Western counterparts.AIM To conduct a nationwide population-based study to assess the risk of CLI in UC long-term survivors.METHODS UC survivors,defined as those who survived for longer than 5 years after the diagnosis,were identified and matched at a 1:4 ratio with normal controls.Stratified Cox models were used to assess the risk of CLI.RESULTS From 2000 to 2005,1889 UC survivors who received surgery alone or surgery combined with radiotherapy(RT)were classified into younger(onset ageents with diabetes,hypertension,and receiving hormone replacement therapy(HRT)were more likely to develop CLI.In contrast,the risk of CLI was associated with adjuvant RT,obesity,hypertension,and HRT in the older group.Among the UC survivors,those who were diagnosed at an advanced age(>65 years,aHR=2.48,P=0.011),had hypertension(aHR=2.18,P=0.008)or received HRT(aHR=3.52,P=0.020)were at a higher risk of CLI.CONCLUSION In this nationwide study,we found that the risk factors associated with CLI were similar in both cohorts except for adjuvant RT that was negligible in the younger group,but positive in the older group.Among the survivors,hypertension,advanced age,and HRT were more hazardous than RT.Secondary prevention should include CLI as a late complication in UC survivorship programs.

关键词： Uterine cancer Critical limb ischemia Radiotherapy Survivorship

施氏矿物形成转化的环境意义及其在环境工程领域的应用研究进展

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四川环境 2024年第2期43卷 85-92页

作者：包艳萍黄恩惠刘振聪杨富国徐亦扬佛山科学技术学院环境与化学工程学院广东佛山528000 佛山市土壤污染修复工程技术研究中心广东佛山528000

施氏矿物是一种高铁硫酸盐矿物,对调控酸性硫酸盐环境中重(类)金属的迁移转化有重要意义。同时施氏矿物具有很强的重(类)金属吸附能力及具备催化性能,其在环境工程领域有广阔的应用前景。对施氏矿物的形成转化及影响因素,以及施氏矿物... 详细信息

施氏矿物是一种高铁硫酸盐矿物,对调控酸性硫酸盐环境中重(类)金属的迁移转化有重要意义。同时施氏矿物具有很强的重(类)金属吸附能力及具备催化性能,其在环境工程领域有广阔的应用前景。对施氏矿物的形成转化及影响因素,以及施氏矿物形成转化过程中重(类)金属的固定及再迁移的研究进行了综述;另外,进一步阐述施氏矿物在环境工程领域中的应用,为今后AMD污染环境的治理,以及施氏矿物在环境工程领域的应用研究提供参考。

关键词：施氏矿物重(类)金属再分配催化作用

Optimized air-ground data fusion method for mine slope modeling

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Journal of Mountain Science 2024年第6期21卷 2130-2139页

作者： LIU Dan huang Man TAO Zhigang HONG Chenjie WU Yuewei FAN en YANG Fei State Key Laboratory for Tunnel engineering China University of Mining and Technology(Beijing)Beijing 100083China Department of Civil engineering Shaoxing UniversityShaoxing 312000China Department of Computer Science and engineering Shaoxing UniversityShaoxing 312000China

Refined 3D modeling of mine slopes is pivotal for precise prediction of geological hazards.Aiming at the inadequacy of existing single modeling methods in comprehensively representing the overall and localized characteristics of mining slopes,this study introduces a new method that fuses model data from Unmanned aerial vehicles(UAV)tilt photogrammetry and 3D laser scanning through a data alignment algorithm based on control points.First,the mini batch K-Medoids algorithm is utilized to cluster the point cloud data from ground 3D laser scanning.Then,the elbow rule is applied to determine the optimal cluster number(K0),and the feature points are extracted.Next,the nearest neighbor point algorithm is employed to match the feature points obtained from UAV tilt photogrammetry,and the internal point coordinates are adjusted through the distanceweighted average to construct a 3D model.Finally,by integrating an engineering case study,the K0 value is determined to be 8,with a matching accuracy between the two model datasets ranging from 0.0669 to 1.0373 mm.Therefore,compared with the modeling method utilizing K-medoids clustering algorithm,the new modeling method significantly enhances the computational efficiency,the accuracy of selecting the optimal number of feature points in 3D laser scanning,and the precision of the 3D model derived from UAV tilt photogrammetry.This method provides a research foundation for constructing mine slope model.

关键词： Air-ground data fusion method Mini batch K-Medoids algorithm Ebow rule Optimal cluster number 3D laser scanning UAV tilt photogrammetry

Factors influencing willingness to participate in ophthalmic clinical trials and strategies for effective recruitment

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International Journal of Ophthalmology(english edition) 2024年第1期17卷 157-163页

作者： Jia-en Liang Meng-Yin Liang en-en Zhang Yu-Ying Peng Ling-Ling Chen Jia-Yu Deng Ting Lin Jun Fu Jia-Ni Zhang Shao-Li Li Fei Li Hui-Ming Xaio Wen-Min huang Yu-Hong Liu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangdong Provincial Clinical Research Center for Ocular DiseasesGuangzhou 510060Guangdong ProvinceChina

AIM:To explore the factors influencing individuals’willingness to participate in ophthalmic clinical trials.METHODS:A questionnaire survey was conducted from January to April 2021 among patients and their family members at Zhongshan Ophthalmic Center,Sun Yat-sen University,in Guangzhou,China.The survey gathered data on respondents’willingness,demographic and socioeconomic profiles,as well as their reasons and concerns regarding engagement in clinical trials.RESULTS:Of the 1078 residents surveyed(mean age 31.2±13.1y;65.8%females)in Guangzhou,749(69.5%)expressed a willingness to participate in future ophthalmic clinical trials.Specific characteristics associated with greater willingness included a younger age,lower annual income,higher education,prior participation experience,previous ophthalmic treatment,and a better understanding of clinical trials.With the exception of age,these characteristics were significantly linked to a higher willingness.The primary barrier to participation,expressed by 64.8%of those willing and 54.4%of those unwilling,was“Uncertain efficacy”.In terms of motivations,the willing group ranked“Better therapeutic benefits”(35.0%),“Professional monitoring”(34.3%),and“Trust in healthcare professionals”(33.1%)as their top three reasons,whereas the unwilling participants indicated“Full comprehension of the protocol”(46.2%)as the key facilitator.CONCLUSION:This study reveals a substantial willingness to participate in ophthalmic clinical trials and demonstrates the predictive role of demographic and socioeconomic factors.Variations in motivators and concerns between willing and unwilling participants highlight the significance of tailored recruitment strategies.Importantly,the need for and trust in healthcare professionals stand out as powerful motivations,underscoring the importance of enhancing physician-patient relationships,adopting patient-centered communication approaches,and addressing individualized needs to improve accrual rates.

关键词： participation willingness recruitment strategy ophthalmic clinical trial