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Mini-cutting technique for Khaya anthotheca: selection of suitable IBA concentration and nutrient solution for its vegetative propagation

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Journal of Forestry Research 2018年第1期29卷 73-84页

作者： Joamir Barbosa Filho Maria Angélica Di Carvalho Leandro Silva de Oliveira enéas ricardo konzen Gilvano Ebling Brondani College of Forestry Federal University of Mato Grosso(UFMT) Institute of Agronomic Sciences Federal University of Minas Gerais (UFMG) Department of Forest Sciences Federal University of Lavras (UFLA)

Khaya anthotheca is a hardwood species from Africa and recently introduced to Brazil. This species yields high-quality wood for diverse applications, but little has been done toward an effective propagation method for large-scale production in nurseries. We evaluated the effects of different concentrations of macro and micronutrients（i.e., 100, 50 and 25% of the concentration of a referenced solution） combined with indole-3-butyric acid（IBA） at 0 and 2 g L-1 on the survival rate of mini-stumps and the survival and adventitious rooting of K. anthotheca mini-cuttings. The mini-stumps were grown from a source of seeds imported from Ghana, West Africa. The ministumps survived at a high rate（97%）. Consistently high shoot yields were obtained from the mini-stumps（average of 1.8 per mini-stump）. High survival percentage of minicuttings and adventitious rooting were observed when solutions with 100 and 50% of the nutrients concentration were combined with 2 g L-1 IBA. Histological sections analyzed through optical microscopy indicated the tissues from mini-cutting stems were juvenile, which might have stimulated adventitious rooting. These results have important implications for further work aimed at establishing propagation strategies for K. anthotheca, which are of prime importance for assisting breeding programs of this species.

关键词： African mahogany Seminal mini-garden Adventitious rooting Juvenile tissue Mini-stump

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Evaluation of total protein,peroxidase,and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

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Journal of Forestry Research 2023年第5期34卷 1563-1576页

作者： Júlio Cézar Tannure Faria Caius Ribeiro-Kumara William Macedo Delarmelina Filipe Aiura Namorato Dione Richer Momolli Anderson Cleiton José enéas ricardo konzen Dulcinéia de Carvalho Gilvano Ebling Brondani Department of Forestry and Wood Sciences Federal University of Espírito SantoJerônimo MonteiroEspírito SantoBrazil Department of Forest Sciences University of HelsinkiHelsinkiFinland Federal Institute of Education Science and Technology of Espírito SantoIbatibaEspírito SantoBrazil Department of Soil Science Federal University of LavrasLavrasMinas GeraisBrazil Department of Forest engineering Federal University of Santa MariaSanta MariaRio Grande Do SulBrazil Department of Forestry Sciences Federal University of LavrasLavrasMinas GeraisBrazil Department Interdisciplinary Federal University of Rio Grande Do SulImbéRio Grande Do SulBrazil

Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species,hampering clonal forestry *** study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile,peroxidase activity,macro-and micronutrient contents,and adventitious rooting of mini *** analyses were performed on *** shoots which were successfully obtained by seminal and grafting propagation,micropropagation with epicormic shoots,and indirect *** four mature trees used in the propagation,tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or *** individuals from each technique were randomly selected and transferred to a semi-hydroponic"channel"*** four weeks in the seedbed,the total protein,peroxidase activity,nutrient content and rooting of the mini cuttings were ***-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques,as revealed by two distinct *** obtained by micropropagation with epicormic shoots showed the highest peroxidase activity,while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity.A portable X-ray fluorescence spectroscope(pXRF)identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation,and by indirect *** analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation(75%)followed by indirect organogenesis(35%).Based on principal component analysis,it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis was associated with phosphorous,sulphur,and potassium

关键词： Adventitious rooting Juvenility Reinvigoration Rejuvenation Ontogenetic age

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