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The Fold-Illuminator:A low-cost,portable,and disposable incubator-illuminator device

Synthetic and Systems Biotechnology 2021年第2期6卷 95-101页

作者： Logan R.Burrington Emran Baryal Katherine Hui emmett lambert Sarah T.Harding Javin P.Oza Chemistry and Biochemistry Department College of Science and MathCalifornia Polytechnic State UniversitySan Luis ObispoCA93407USA Mechanical Engineering Department College of EngineeringCalifornia Polytechnic State UniversitySan Luis ObispoCA93407USA

Fluorescent reporters have revolutionized modern applications in the fields of molecular and synthetic biology,enabling applications ranging from education to point-of-care *** advancements in these fields have primarily focused on improving reaction conditions,the development of new applications,and the broad dissemination of these ***,field and classroom-based applications have remained limited in part due to the nature of fluorescent signal detection,which often requires the use of costly lab equipment to observe and quantify fluorescence *** without access to laboratory equipment rely on qualitative assessments of fluorescence,a process that remains highly variable from user-to-user even within the same *** overcome this challenge,we have developed a foldable illuminator and incubator device to support field-applications of synthetic biology-based biosensors for education and *** Fold-Illuminator is an affordable,portable,and recyclable device that allows for the visible detection of fluorescent *** Fold-Illuminator’s design allows for assembly in under 10 min,a user can then utilize the optional heating element to incubate biochemical reactions and visualize fluorescence outputs in a defined and light-controlled *** LED strips and light-filtering screens provide modularity to pair with the fluorescence wavelengths of *** user can then unfold the device for convenient storage,transport,or even *** cost for the Fold-Illuminator is$5.58 USD and is compatible with an optional heating element for an additional$3.98 cost,with potential for further reductions in cost for larger ***-source templates for cutting device parts from paper stock are provided for both printing and cutting by hand;cutting can also be achieved with consumer-grade smart cutting machines such as the Cricut®.Combined with the broad applications of fluorescent reporters

关键词： Illuminator Incubator Fluorescent reporter Education Diagnostics Frugal science

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