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World Journal of Vaccines 2012年第1期2卷 27-35页

作者： Lani Indrawati Jon H. Heinrichs emily P. Wen Julie m. Skinner Vaccines Research Merck Research Laboratories West Point USA

Neisseria meningitidis is a gram negative diplococcal bacterium. Worldwide, N. meningitidis is the leading cause of bacterial meningitis and sepsis, with five serogroups (A, B, C, Y, and W-135) responsible for the majority of the disease. multivalent (A, C, Y, and W-135) polysaccharide and conjugate vaccines have been licensed in the United States and elsewhere and are widely available. We have developed a multi-plexed electrochemiluminescent assay to quantitate serum antibody responses to meningococcal polysaccharides A, C, Y, and W-135 to allow for rapid evaluation of li- censed and investigational vaccines. A 96-well plate containing a carbon electrode arrayed with polysaccharides A, C, Y, and W-135 on separate spots within each well has been developed for simultaneous detection of polysaccharidespecific antibodies in serum samples from vaccinated individuals. The assay conditions were optimized using the anti-meningococcal serogroup A/C reference serum pool, CDC 1992 (NIBSC 99/706), through evaluation of plate types, coating polysaccharide concentrations, and blocking and serum diluent buffers. Comparison of single and multiplex assays demonstrated the sensitivity, specificity, and speed of the multi-plex format for the quantification of serum antibody responses to N. meningitidis polysaccharides A, C, Y and W-135.

关键词： Neisseria meningitidis Electrochemiluminescent Detection Serum Antibody

Vertebral Osteomyelitis Due to <i>Candida</i>Species: A Cohort Study and Review of the Literature

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Open Journal of Orthopedics 2013年第2期3卷 81-89页

作者： Amanda Ramos Paul m. Huddleston Robin Patel emily Vetter Elie F. Berbari Department of Laboratory medicine and Pathology Mayo Clinic Rochester USA Department of Orthopedic Surgery Mayo Clinic Rochester USA Division of Infectious Diseases Mayo Clinic Rochester USA. mayo medical School Mayo Clinic Rochester USA

Objective: To describe the demographics and outcome of patients with candidal vertebral osteomyelitis (CVO). CVO is a rare and frequently misdiagnosed condition. It may lead to destruction of the vertebral bodies, spinal cord compression and neurological deficits. methods: The medical records of all patients diagnosed with CVO at our institution between 01/01/1990-12/31/2009 were reviewed. The cumulative probability of treatment success was assessed by the Kaplan-meier survival method. Patients were followed until death, failure, or loss of follow-up. Results: Nine patients developed CVO during the 20 year study period. The cervical spine was involved in 5 cases. Seven patients presented with mechanical-type pain, while 2 patients had an elevated temperature at diagnosis. A contiguous infection between the upper airways and the cervical spine was present in 4 patients. One patient presented with concomitant candidemia. Candida albicans, Candida parapsilosis, and Candida glabrata were cultured in 3 of 9 cases respectively. Eight of 9 were treated with azole-based therapy. Patients were followed for an average of 20 months (range 1 - 75 months). The cumulative incidence of success was 66% ± 19% at 1 year and 55% ± 20% at 2 years of follow-up. Conclusions: CVO presents insidiously and is associated with a long duration of symptoms. It most frequently affects the cervical spine and is associated with a poor outcome.

关键词： Vertebral Osteomyelitis Candida Back Pain Antifungal Therapy

糖尿病流行病学研究中应用OGTT资料评估胰岛β细胞功能的可能性——468例非糖尿病Pima印第安人葡萄糖钳研究资料分析

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中华内分泌代谢杂志 2003年第1期19卷 8-12页

作者：李光伟 bennett PH 北京中日友好医院内分泌科 100029 Phoenix Epidemiology and Clinical Research Branch NIDDKNIHPhoenixArizonausa85014

目的　探讨以口服葡萄糖耐量试验 (OGTT)的简单参数在糖尿病流行病研究中评估胰岛 β细胞功能的可能性。方法　美国Pima印第安人糖耐量正常者 332例、糖耐量低减者 1 36例参与本试验。各例均做静脉葡萄糖耐量试验 (IVGTT) ,OGTT及正葡... 详细信息

目的　探讨以口服葡萄糖耐量试验 (OGTT)的简单参数在糖尿病流行病研究中评估胰岛 β细胞功能的可能性。方法　美国Pima印第安人糖耐量正常者 332例、糖耐量低减者 1 36例参与本试验。各例均做静脉葡萄糖耐量试验 (IVGTT) ,OGTT及正葡萄糖钳试验。计算Homa IR ,Homa β ,第一时相胰岛素分泌量 (AIR) ,胰岛素曲线下面积 ,ΔI30 /ΔG30 ,胰岛素介导的葡萄糖代谢率 (m ,mg·kg- 1 ·min- 1 )。从基本病理生理学概念推导新 β细胞功能指数mBCI=(FINS×FPG) / (PG2h +PG1h- 2×FPG) (FINS :空腹胰岛素 ;FPG :空腹血糖 ;PG1h和PG2h分别为OGTT 1h和 2h的血糖 )。以线性回归分析正葡萄糖钳技术测定的胰岛素敏感性与不同的β细胞功能指数的组合对血糖水平的贡献 ,探讨以OGTT的简单参数评估β细胞功能的可能性。结果　 (1 )调整m后 ,IGT组AIR、ΔI30 /ΔG30 、Homa β和mBCI与OGTT 2h血糖水平的偏相关系数分别为 - 0 .30、- 0 .30、- 0 .2 9及 - 0 .37(P均 0 .0 5)、0 .0 1 (P >0 .0 5)、- 0 .30 (P m +AIR ,m +ΔI30 /ΔG30 ,m +Homa β,m +mBCI为自变量做线性回归分析 ,显示m

关键词：糖尿病流行病学胰岛 β细胞功能评估葡糖耐量试验

Impact of deforestation on habitat connectivity thresholds for large carnivores in tropical forests

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Ecological Processes 2017年第1期6卷 214-224页

作者： miriam A.Zemanova Humberto L.Perotto-Baldivieso emily L.Dickins Andrew B.Gill John P.Leonard David B.Wester School of Energy Environment and AgrifoodCranfield UniversityCranfield MK430ALUK Institute of Ecology and Evolution University of BernBaltzerstrasse 63012 BernSwitzerland Caesar Kleberg Wildlife Research Institute Texas A&M University-Kingsville700 University Blvd.MSC 218KingsvilleTX 78363USA museo de Historia Natural Noel Kempff mercado Universidad Autónoma Gabriel Rene MorenoAvenida Irala 565Santa Cruz de la SierraBolivia Hensmans Farm Nearton EndSwanbourne MK170SLUK Cape Eleuthera Institute EleutheraBahamas

Introduction:Deforestation significantly impacts large carnivores that depend on large tracts of interconnected forest habitat and that are sensitive to human *** the relationship between habitat use and spatial distribution of such species across human modified landscapes is critical when planning effective conservation *** study assessed the presence of potential landscape connectivity thresholds resulting from habitat fragmentation associated with different deforestation patterns using a scale-based approach that links species-specific home ranges with the extent of anthropogenic *** objectives were(1)to quantify the spatial and temporal distribution of natural vegetation for five common deforestation patterns and(2)to evaluate the connectivity associated with these patterns and the existence of potential thresholds affecting jaguar *** Bolivian lowlands,located within jaguar conservation units,were analysed with landscape metrics to capture the spatial and temporal changes within deforested areas and to determine potential impact on jaguar connectivity and connectivity thresholds for ***:Over the period of 1976–2005,the amount of natural vegetation has decreased by more than 40%in all locations with the biggest changes occurring between 1991 and *** spatial structure around jaguar locations showed that jaguars used areas with mean proportion of natural areas=83.14%(SE=3.72%),mean patch density=1.16 patches/100 ha(SE=0.28 patches/100 ha),mean patch area=616.95 ha(SE=172.89 ha)and mean edge density=705.27 m/ha(SE=182.19 m/ha).We observed strong fragmentation processes in all study locations,which has resulted in the connectivity of jaguar habitat decreasing tomentation and connectivity for carnivores within the extent of human-modified la

关键词： Bolivian lowlands Habitat fragmentation Landscape metrics Panthera onca Threshold

moving fast, thinking fast:The relations of physical activity levels and bouts to neuroelectric indices of inhibitory control in preadolescents

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Journal of Sport and Health Science 2019年第4期8卷 301-314页

作者： Dominika m.Pindus Eric S.Drollette Lauren B.Raine Shih-Chun Kao Naiman Khan Daniel R.Westfall morgan Hamill Rebecca Shorin emily Calobrisi Dinesh John Arthur F.Kramer Charles H.Hillman Department of Kinesiology and Community Health University of Illinois at Urbana-ChampaignUrbanaIL 61801USA School of Health and Human Sciences University of North Carolina GreensboroGreensboroNC 6170USA Department of Psychology Northeastern UniversityBostonMA 02115USA Department of Health and Kinesiology Purdue UniversityWest LafayetteIN 47907USA Department of Health Sciences Northeastern UniversityBostonMA 02115USA Department of Physical Therapy Movement&Rehabilitation SciencesNortheastern UniversityBostonMA 02115USA

Background:Structured vigorous physical activity(VPA)can improve cognitive control in children,but studies relating daily physical activity(PA)to cognitive control have yielded conflicting *** objectively measured daily PA summarizes all occurrences of PA within a registered period,a minimum duration of continuous PA is required for registration of a PA *** brief bouts of high-intensity PA can account for a large proportion of children’s daily activity-related energy expenditure,this study assessed whether daily and bouted VPA were selectively related to cognitive control in preadolescents relative to other PA ***:A total of 75 children between the ages of 8 and 10 years(49%girls)wore an ActiGraph wGT 3 X+on the hip for 7 *** acceleration signal from the vertical axis was summarized over 1 s,5 s,and 15 s *** and boutecd modercate PA,moderate-to-vigorous PA,and VPA were *** bouts were expressed as the frequency and time spent in 2 different continuous PA bouts,one lasting≥10 s and the other lasting≥30 s at a given *** control was assessed using behavioral responses to a modified flanker task(mean reaction time(RTmean)and accuracy).Attentional resource allocation and co gnitive processing speed were measured using the amplitude and latency of the P3 component of event-related brain potentials,*** between PA,behavioral indices of inhibitory control,P3 amplitude,and latency were assessed using hierarchical regression ***:Daily VPA was not related to RTmean or accuracy on either congruent or incongruent *** contrast,more time spent in VPA bouts lasting≥30 s predicted shorter P3 latency across epochs and flanker congruencies(allβ≤-0.24,all p≤0.04).The associations between shorter P3 latency and the time spent in moderate-to-vigorous PA bouts lasting≥30 s were less consistent and largely limited to congruent trials(congruent:β(-0.3 1,-0.34)).No significant asso

关键词： Accelerometry Bouts Children ERPs Inhibitory control P3 Physical activity

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急性巨核细胞白血病(m7)

国际输血及血液学杂志 1989年第1期12卷 17-18页

作者： matsuoT bennett Jm 张效民

最近被列入急性白血病FAB分型中的急性巨核细胞白血病或m7,过去被称为“急性骨髓纤维化”,“恶性骨髓硬化“和”急性骨髓增生异常伴髓外化生”等。虽然命名不同,但诊断标准基本上是相同的:起病急,病程进展快,无特殊症状或器官肿大;全血... 详细信息

最近被列入急性白血病FAB分型中的急性巨核细胞白血病或m7,过去被称为“急性骨髓纤维化”,“恶性骨髓硬化“和”急性骨髓增生异常伴髓外化生”等。虽然命名不同,但诊断标准基本上是相同的:起病急,病程进展快,无特殊症状或器官肿大;全血细胞减少,外周血出现原始细胞,红细胞形态无显著改变;骨髓中网状纤维增生,有核细胞增生程度增加,原始细胞和不典型的形态奇特的巨核细胞增多。然而,近来的研究揭示骨髓纤维化(骨纤)可能继发于原始巨核细胞的增殖。临床上可见化疗后或骨髓移植后骨纤得到缓解。细胞遗传学研究发现,体外培养时骨髓成纤维细胞和淋巴细胞显示正常核

关键词：急性巨核细胞白血病原始巨核细胞 m7

Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments

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Cellular & molecular Immunology 2012年第2期9卷 163-174页

作者： Ashley m Trama Zoie E Holzknecht Anitra D Thomas Kuei-Ying Su Sean m Lee emily E Foltz Sarah E Perkins Shu S Lin William Parker Department of Surgery Duke University Medical Center Durham NC 27710 USA Cardiff School of Biosciences Cardiff CFIO 3AX UK Department of Immunology Duke University Medical Center Durham NC 27710 USA

The immune systems of wild rats and of laboratory rats can been utilized as models of the human immune system in pre-industrial and post-industrial societies, respectively. In this study, lymphocyte phenotypes in wild rats were broadly characterized, and the results were compared to those obtained by us and by others using cells derived from various strains of laboratory rats. Although not expected, the production of regulatory T cells was not apparently different in wild rats compared to laboratory rats. On the other hand, differences in expression of markers involved in complement regulation, adhesion, signaling and maturation suggest increased complement regulation and decreased sensitivity in wild-caught rats compared to laboratory rats, and point toward complex differences between the maturation of T cells. The results potentially lend insight into the pathogenesis of post-industrial epidemics of allergy and autoimmune disease.

关键词： allergy autoimmunity biome hygiene immune regulation

大学与中小学伙伴协作:美国教师教育中的实践

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当代教师教育 2008年第4期1卷 6-11页

作者： missy m. bennett 陈佑清乔治亚南方大学教育学院华中师范大学教育学院湖北武汉430079

美国职前教师教育专业计划是与教师准备任教的学生年龄段及科目相对应的,其中特别强调师范生获取大量的教育现场经验。美国在职教师教育的特点主要有,安排经验丰富的教师指导新教师;在职教师必须进行持续的学习。为改进教师教育的质量,... 详细信息

美国职前教师教育专业计划是与教师准备任教的学生年龄段及科目相对应的,其中特别强调师范生获取大量的教育现场经验。美国在职教师教育的特点主要有,安排经验丰富的教师指导新教师;在职教师必须进行持续的学习。为改进教师教育的质量,美国普遍推行建立大学与中小学之间的伙伴协作关系,其采取的主要形式是建立专业发展学校。专业发展学校的使命有:为教师候选人提供专业准备、促进指导教师的专业发展、开展旨在改进教学实践的探究、提高学生的学业成绩。在美国,大学与中小学之间的伙伴协作既取得了明显的成效,也面临着多方面的挑战。

关键词：美国教师教育大学与中小学伙伴协作宗旨成效挑战

关于空腹血糖、空腹胰岛素乘积的倒数在流行病学研究中应用的补充说明

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中华糖尿病杂志（1006-6187） 2005年第4期13卷 247-249页

作者：李光伟 bennett PH 卫生部中日友好医院内分泌科 Phoenix Epidemiology and Clinical Research Branch 1550E.Indian School Road PhoenixArizona 85014

高胰岛素正糖钳夹技术可以测定活体的胰岛素敏感性,但它并不适用于大规模流行病学研究。流行病学研究需要简单的胰岛素抵抗测定法。本文补充报告在空腹血糖(FPG)(75～306mg/dl或4.2～17.1mmol/L)及空腹胰岛素(FIns)(9.7～120mU/L)范围... 详细信息

高胰岛素正糖钳夹技术可以测定活体的胰岛素敏感性,但它并不适用于大规模流行病学研究。流行病学研究需要简单的胰岛素抵抗测定法。本文补充报告在空腹血糖(FPG)(75～306mg/dl或4.2～17.1mmol/L)及空腹胰岛素(FIns)(9.7～120mU/L)范围很宽的Pima印第安人群中,正糖钳夹技术测定的胰岛素介导的葡萄糖代谢率(m)与涉及FPG、FIns的多种复合的胰岛素敏感指数的相关性:胰岛素作用指数(IAI)=1/(FPG×FIns)在非糖尿病人群及2型糖尿病人群都与m显著正相关(r>0.7,P=0.0001),而且这两者的相关性强于m与其他指数如FIns或FPG/FIns比值的相关性,也不弱于m与糖负荷后3～5个时间点的血糖、胰岛素曲线下面积乘积的相关性。IAI的五分变量分布情况表明有90.4%的IAI落在所预测的m值五分变量区域或与之相邻的一个五分变量区域之内。1/FPG×FIns虽相对简单但确实与机体的胰岛素敏感性密切相关,它可以做为胰岛素敏感指数在流行病学研究中应用。

关键词：空腹血糖空腹胰岛素乘积流行病学正糖钳夹技术胰岛素敏感性指数糖尿病

multi-temporal NDVI analysis using UAV images of tree crowns in a northern mexican pine-oak forest

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林业研究:英文版 2023年第6期34卷 1855-1867页

作者： JoséLuis Gallardo-Salazar marcela Rosas-Chavoya marín Pompa-García Pablito marcelo López-Serrano emily García-montiel Arnulfo meléndez-Soto Sergio Iván Jiménez-Jiménez Instituto de Investigaciones Agropecuarias y Forestales Universidad Michoacana de San Nicolás de Hidalgo(UMSNH)58330 MoreliaMichoacánMexico Programa Institucional de Doctorado en Ciencias Agropecuarias y Forestales Universidad Juárez del Estado de DurangoRío Papaloapan y Blvd.DurangoValle del Sur s/n34120 DurangoDurangoMéxico Facultad de Ciencias Forestales Universidad Juárez del Estado de DurangoRío Papaloapan y Blvd.DurangoValle del Sur s/n34120 DurangoDurangoMéxico Instituto de Silvicultura e Industria de la madera Universidad Juárez del Estado de DurangoBoulevard del Guadiana 501Ciudad UniversitariaTorre de Investigación34120 DurangoMéxico INIFAP-CENID RASPA Centro Nacional de Investigación Disciplinaria en Relación Agua-Suelo-Planta-Atmósfera Margen derecha canal Sacramento km 6.5Zona industrial35140 Gómez PalacioDurangoMexico

The use of unmanned aerial vehicles(UAV)for forest monitoring has grown significantly in recent years,providing information with high spatial resolution and temporal *** with multispectral sensors allow the use of indexes such as the normalized difference vegetation index(NDVI),which determines the vigor,physiological stress and photo synthetic activity of *** study aimed to analyze the spectral responses and variations of NDVI in tree crowns,as well as their correlation with climatic factors over the course of one *** study area encompassed a 1.6-ha site in Durango,mexico,where Pinus cembroides,Pinus engelmannii,and Quercus grisea *** images were acquired with UAV and information on meteorological variables was obtained from NASA/POWER *** ANOVA explored possible differences in NDVI among the three *** correlation was performed to identify the linear relationship between NDVI and meteorological *** differences in NDVI values were found at the genus level(Pinus and Quercus),possibly related to the physiological features of the species and their *** grisea had the lowest NDVI values throughout the year which may be attributed to its sensitivity to relative humidity and *** the use of UAV with a multispectral sensor for NDVI monitoring allowed genera differentiation,in more complex forest analyses hyperspectral and LiDAR sensors should be integrated,as well other vegetation indexes be considered.

关键词： multispectral images Normalized diff erence Vegetation index Phenology Unmanned aerial vehicles multitemporal analysis