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Study of Construction Techniques and Hygro-Thermal Behavior of a Vernacular Earth Building in a Humid Tropical Climate

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Journal of Building Construction and Planning Research 2023年第3期11卷 69-85页

作者： Mouhamadou Nabi Kane Mapathe Ndiaye emilie Pinard University College of Architecture Dakar (CUAD) University of Thies Thies Senegal Laboratory of Mechanics and Modeling University of Thies Thies Senegal McEwen School of Architecture Laurentian University Sudbury Canada

This study analyzes the know-how of local communities, to draw on techniques that make contemporary buildings more energy efficient. The impluvium hut in the locality of Enampore, Casamance, Southern Senegal, served as the object of study. The hut, including several rooms, is entirely built with earthen walls, earthen floor, earthen ceiling, covered by a double straw roof and its central courtyard. A room noted (L) and a semi-opened living space were chosen as spaces for hygro-thermal experimentation. The hottest average temperature obtained respectively in the room (L) and in the living space is 25.5˚C and 27˚C when outside is about 34˚C. The thermal amplitude inside room (L) is 0.88˚C, in semi-opened living space, is 2.6˚C and outside is 9.5˚C. With these results we can say that room (L) undergoes very low temperature variations and that there is no need to air-condition in the enclosure. The thermal amplitude makes it possible to see the influence of the earthen walls on the interior temperature and its regularity compared to the fluctuation of the external temperature. The thermal inertia of the building walls was characterized using also the time lag and the decrement factor. They was respectively 7.0 H and 0.093 for the room (L). With this result we can say that this material has a high thermal inertia. For humidity, it is high around 78.5% in the room (L), 66.0% at the semi-open living room, when it is 59.0% outside. Through this study, it is possible that the revalorization of vernacular architecture can be an alternative to reduce the energy consumption of buildings.

关键词： Time Lag Decrement Factor Thermal Inertia Double Skin Impluvium

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丙型肝炎病毒E2包膜糖蛋白背层结构域抑制病毒进入的表征及优化

丙型肝炎病毒E2包膜糖蛋白背层结构域抑制病毒进入的表征及优化

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作者： emilie CARLOT 中国科学院大学(中国科学院上海巴斯德研究所)

学位级别：硕士

丙型肝炎病毒(HCV)是一种小型的单股正链带包膜的RNA病毒,系黄病毒科,丙型肝炎病毒属。HCV可通过血液传播,并在肝细胞中复制造成肝脏疾病,被称为丙肝。全世界范围内,大约有1.85亿人感染了丙肝病毒。虽然机体可以清除入侵的病毒颗粒,但... 详细信息

丙型肝炎病毒(HCV)是一种小型的单股正链带包膜的RNA病毒,系黄病毒科,丙型肝炎病毒属。HCV可通过血液传播,并在肝细胞中复制造成肝脏疾病,被称为丙肝。全世界范围内,大约有1.85亿人感染了丙肝病毒。虽然机体可以清除入侵的病毒颗粒,但是患者在最初感染HCV后通常伴随有慢性肝炎,有时还会恶化为肝硬化甚至肝癌。随着对丙肝病毒的结构及其生活史的深入了解,现已开发出直接抗病毒药物(DAAs)等,其可在体外有效抑制HCV感染。然而这些药物的使用仍然存在局限性,目前仍然迫切需要针对丙肝生活史的不同阶段制定出多重治疗策略。HCV复制周期的第一步就是吸附和侵入肝细胞,这为治疗提供了有效的靶点。HCV进入细胞的过程受细胞和病毒间的多种因素所调控,其中HCV E1和E2包膜糖蛋白会在病毒表面形成具有功能的异质二聚体。目前尚不清楚E1和/或E2中哪个是能够引导膜融合的蛋白。虽然E1在其中发挥的作用仍不明确,但现已知道E2能够结合细胞受体。对丙肝E2包膜糖蛋白的最新的研究表明,E2包膜糖蛋白的胞外域是呈球形的非延伸的折叠,并分为不同的两层:前层和中央基因型间结构域;和一个后层结构域(E2-Bld)。现已发现E1和E2-Bld之间的关键相互作用,为探究该相互作用在病毒进入细胞过程中扮演的重要角色,我们设计了E2-Bld多肽来抑制这种E1-E2-Bld相互作用。体外实验表明,可溶性E2-Bld多肽能抑制体外培养的活病毒(HCVcc)的复制和HCV假病毒(HCVpp)的进入,是研究HCV进入可靠的模型。有趣的是,这种E2-Bld多肽还能够抑制HCV在人源化肝脏小鼠体内的复制,人源化肝脏小鼠是研究HCV体内复制的有效模型,可以更全面地探究HCV的多样性。在此背景下,本项目旨在鉴定这种E2-Bld多肽的抑制作用。在最初的实验中,我们构建了一系列质粒来优化对E2-Bld多肽的纯化效果和功能发挥。通过改变多聚组氨酸标签的长度来增强纯化效果,通过突变半胱氨酸去除二硫键从而避免E2-Bld多聚化造成的功能破坏。这些质粒在哺乳动物细胞中能够表达E2-Bld多肽,但是表达效率不高。为提升产量,我们在第二步实验中,将E2-Bld多肽重新克隆并于昆虫细胞中表达。不幸的是,E2-Bld的产量并没有得到明显的提升。因此,为了不使用多肽免疫就能获得相应抗体,我们决定采取核酸免疫策略来避免蛋白纯化步骤。将已构建的各种质粒分别通过电转注射到小鼠的肌肉组织以表达E2-Bld多肽,然后收集这些小鼠血清并用HCV J6毒株开展抗原抗体中和实验。有趣的是,我们发现小鼠在不同的E2-Bld质粒免疫注射后产生了特异性免疫应答。总之,我们希望这些结果能有助于更好地了解HCV进入细胞的机制,并从长远来看,有望据此开发出抗HCV感染的疫苗。

关键词：丙型肝炎病毒 E2包膜糖蛋白后层结构域抑制感染组蛋白标签

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Psychiatric outcomes in outpatients affected by long COVID:A link between mental health and persistence of olfactory complaint

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World Journal of Psychiatry 2024年第4期14卷 507-512页

作者： Victoria Metelkina-Fernandez Louise-emilie Dumas Clair Vandersteen David Chirio Auriane Gros Arnaud Fernandez Florence Askenazy Valeria Manera Department of Psychiatry Nice University HospitalNice 06000France Department of Child and Adolescent Psychiatry UniversitéCôte d’AzurNice 06200France ENT Head and Neck InstituteNice 06100France Department of Infectiology Nice University HospitalNice 06200France Department of Orthophony UniversitéCôte d’AzurNice 06100France Cobtek UniversitéCôte d’AzurNice 06100France

BACKGROUND Anosmia was one of the main symptoms of coronavirus disease 2019(COVID-19).A psychiatric history(i.e.,depression)may be an independent contributor to the risk of COVID-19 diagnosis,and COVID-19 survivors appear to have an increased risk of neuropsychiatric sequelae(bidirectional association).AIM To compare the rate of psychiatric disorder among post-COVID patients without anosmia vs patients with persistent olfactory *** We conducted a prospective case control study from March 2020 to May *** recruited at the ENT department of Nice University Hospital had a subjective olfactory complaint(visual analogue scale)for over 6 wk and a molecular or CT-proven severe acute respiratory syndrome coronavirus 2 diagnosis confirmed by ***-COVID patients without persistent olfactory disorders were recruited at the university hospital infectiology *** medical histories were collected by a psychiatrist during the *** Thirty-four patients with post-COVID-19 olfactory complaints were included in the first group of the *** percent of the patients were female(n=17).The group’s mean age was 40.5±12.9 *** control group included 32 participants,of which 34.4%were female(n=11),and had a mean age of 61.2±12.2 *** rate of psychiatric disorder among post-COVID patients with olfactory complaints was significatively higher(41.7%)than among patients without(18.8%)(χ2=5.9,P=0.015).CONCLUSION The presence of a psychiatric history may constitute a potential risk factor for the development of long COVID due to persistent *** therefore seems important to establish reinforced health monitoring after a COVID 19 infection in at-risk *** prospective,translational,and collaborative studies are needed to extrapolate these results to the general population.

关键词： COVID-19 Anosmia Psychiatry Stress Neuroplasticity Psychiatric history

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Biological mesh reconstruction of the pelvic floor following abdominoperineal excision for cancer:A review

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World Journal of Clinical Oncology 2017年第3期8卷 249-254页

作者： Boris Schiltz Nicolas Christian Buchs Marta Penna Cosimo Riccardo Scarpa emilie Liot Philippe Morel Frederic Ris Division of Visceral Surgery Department of SurgeryUniversity Hospitals of Geneva Department of Colorectal Surgery Churchill HospitalOxford University Hospitals

Extralevator abdominoperineal excision and pelvic exenteration are mutilating operations that leave wide perineal *** large wounds are prone to infection and perineal herniation,and their closure is a major concern to most *** approaches to the perineal repair exist,varying from primary or mesh closure to myocutaneous *** technique has its own associated advantages and potential complications and the ideal approach is still *** the present study,we reviewed the current literature and our own local data regarding the use of biological mesh for perineal wound *** evidence suggests that the use of biological mesh carries an acceptable risk of wound complications compared to primary closure and is similar to flap *** addition,the rate of perineal hernia is lower in early follow-up,while long-term hernia occurrence appears to be similar between the different ***,it is an easy and quick reconstruction *** more expensive than primary closure,the cost associated with the use of a biological mesh is at least equal,if not less,than flap reconstruction.

关键词： Biological mesh Rectal cancer Pelvic exenteration Abdominoperineal resection Primary perineal wound closure Perineal wound infection Perineal hernia

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Predictive factors of histological response of colorectal liver metastases after neoadjuvant chemotherapy

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World Journal of Gastrointestinal Oncology 2019年第4期11卷 295-309页

作者： Chloé Serayssol Charlotte Maulat Florence Breibach Fatima-Zohra Mokrane Janick Selves Rosine Guimbaud Philippe Otal Bertrand Suc emilie Berard Fabrice Muscari Department of Digestive Surgery and Liver Transplantation Toulouse-Rangueil University Hospital Department of Pathology Toulouse University Hospital Department of Radiology Toulouse-Rangueil University Hospital Department of Oncology Toulouse-Rangueil University Hospital The Toulouse Research Methodology Support Unit Toulouse University Hospital

BACKGROUND Colorectal cancer is the third most common cancer in men and the second most common in women worldwide. Almost a third of the patients has or will develop liver metastases. Neoadjuvant chemotherapy(NAC) has recently become nearly systematic prior to surgery of colorectal livers metastases(CRLMs). The response to NAC is evaluated by radiological imaging according to morphological *** recently, the response to NAC has been evaluated based on histological criteria of the resected specimen. The most often used score is the tumor regression grade(TRG), which considers the necrosis, fibrosis, and number of viable tumor *** To analyze the predictive factors of the histological response, according to the TRG, on CRLM surgery performed after *** January 2006 to December 2013, 150 patients who had underwent surgery for CRLMs after NAC were included. The patients were separated into two groups based on their histological response, according to Rubbia-Brandt *** on their TRG, each patient was either assigned to the responder(R) group(TRG 1, 2, and 3) or to the non-responder(NR) group(TRG 4 and 5). All of the histology slides were re-evaluated in a blind manner by the same specialized pathologist. Univariate and multivariate analyses were *** Seventy-four patients were classified as responders and 76 as *** postoperative mortality rate was 0.7%, with a complication rate of 38%.Multivariate analysis identified five predictive factors of histological *** were predictive of non-response: More than seven NAC sessions, the absence of a radiological response after NAC, and a repeat hepatectomy(P <0.005). Two were predictive of a good response: A rectal origin of the primary tumor and a liver-first strategy(P < 0.005). The overall survival was 57% at 3 yr and 36% at 5 yr. The disease-free survival rates were 14% at 3 yr and 11% at 5 *** factors contributing to a poor prognosis for disease-free survi

关键词： Colorectal liver metastasis Tumor regression grade Neoadjuvant chemotherapy Liver surgery Histological response Hepatectomy

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Comparative effects of α2δ-1 ligands in mouse models of colonic hypersensitivity

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World Journal of Gastroenterology 2016年第31期22卷 7111-7123页

作者： Mathieu Meleine Ludivine Boudieu Agathe Gelot emilie Muller Amandine Lashermes Julien Matricon Celine Silberberg Vassilia Theodorou Alain Eschalier Denis Ardid Frederic A Carvalho Clermont Université Université d'Auvergne INSERM 1107 Neuro-Dol Université d’Auvergne INSERM 1107 Neuro-Dol INRA EI-Purpan UMR 1054 Neuro-Gastroenterology and Nutrition

AIM: To investigate anti-hypersensitive effects of α2δ-1 ligands in non-inflammatory and inflammationassociated colonic hypersensitivity(CHS) mouse ***: To induce an inflammation-associated CHS, 1% dextran sulfate sodium(DSS) was administered to C57Bl/6J male mice, in drinking water, for 14 d. Regarding the non-inflammatory neonatal maternal separation(NMS)-induced CHS model, wild-type C57BI/6J pups were isolated from their mother from day 2 to day 14(P2 to P14), three hours per day(from 9:00 a.m. to 12:00 p.m.). Colorectal distension was performed by inflating distension probe from 20 μL to 100 μL by 20 μL increment step every 10 s. After a first colorectal distension(CRD), drugs were administered subcutaneously, in a cumulative manner,(Gabapentin at 30 mg/kg and 100 mg/kg; Pregabalin at 10 mg/kg and 30 mg/kg; Carbamazepine at 10 mg/kg and 30 mg/kg) and a second CRD was performed one hour after each ***: The visceromotor response(VMR) to CRD was increased by our NMS paradigm protocol in comparison to non-handled(NH) mice, considering the highest distension volumes(80 μL: 0.783 ± 0.056 mV /s vs 0.531 ± 0.034 m V/s, P < 0.05 and 100 μL: 1.087 ± 0.056 m V/s vs 0.634 ± 0.038 m V/s, P < 0.05 for NMS and NH mice, respectively). In the inflammationassociated CHS, DSS-treated mice showed a dramatic and significant increase in VMR at 60 and 80 μL distension volumes when compared to control mice(60 μL: 0.920 ± 0.079 m V/s vs 0.426 ± 0.100 m V/s P < 0.05 and 80 μL: 1.193 ± 0.097 mV /s vs 0.681 ± 0.094 mV /s P < 0.05 for DSS- and Water-treated mice, respectively). Carbamazepine failed to significantly reduce CHS in both models. Gabapentin significantly reduced CHS in the DSS-induced model for both subcutaneous injections at 30 or 100 mg/kg. Pregabalin s i g n i f i c a n t l y r e d u c e d V M R t o C R D i n t h e n o n-inflammatory NMS-induced CHS model for the acute subcutaneous administration of the highest cumulative dose(30 mg/kg) and significan

关键词： Neonatal maternal separation Dextran sulfate sodium Colonic hypersensitivity mouse models Colorectal distension α2δ-1 ligands

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高砷含水层沉积物矿物学特征及砷的活化

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矿物岩石 2012年第4期32卷 7-11页

作者：黄爽兵 emilie Even 王焰新中国地质科学院水文地质环境地质研究所河北石家庄050061 中国地质大学(武汉)环境学院湖北武汉430074 瑞士纳沙泰尔大学水文地质研究中心

利用X射线衍射和X射线荧光分析、沉积物序列提取试验及矿物饱和度的计算,对采自江汉平原中部沙湖地区典型高砷含水层钻孔沉积物样品矿物学进行了分析,并讨论了控制含水层中砷释放和迁移的地球化学机制。对矿物在沉积物与土壤中的分布及... 详细信息

利用X射线衍射和X射线荧光分析、沉积物序列提取试验及矿物饱和度的计算,对采自江汉平原中部沙湖地区典型高砷含水层钻孔沉积物样品矿物学进行了分析,并讨论了控制含水层中砷释放和迁移的地球化学机制。对矿物在沉积物与土壤中的分布及组成的对比分析,在一定程度上指示了矿物赋存环境和/或高砷水形成的环境背景:土壤与沉积物中高岭石以低于其他3种粘土矿物的含量普遍存在,指示了含水层沉积物形成过程江汉平原存在一定的湿热古气候环境;沉积物绿泥石含量低于土壤中绿泥石,恰恰反映了土壤比沉积物略强的碱性环境;沉积物中黄铁矿的存在,显示了含水层局部的强还原性环境,指示地下水中广泛存在的Fe2+容易与二价硫发生沉淀并结合砷。砷主要以无定形铁锰氧化物结合态(平均在31%以上)形式存在,其次以碳酸盐和有机质结合态存在。无定形铁锰氧化物的还原溶解可能是控制砷迁移到地下水中主要的地球化学机制。相对高含量的绿泥石容易在含水层中发生风化,其溶解过程可以将铁释放到地下水中,从而成为影响地下水中砷活化的潜在因素。

关键词：矿物学特征砷绿泥石饱和指数江汉平原

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Assessment of the Effects of Supplemental Rumen Protected B Vitamins and Choline for Periparturient Cows: A Meta-Analysis of 28 Feeding Studies

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Open Journal of Animal Sciences 2023年第1期13卷 151-165页

作者： Essi Evans emilie Fontaine Ousama AlZahal Chris Gwyn E + E Technical Advisory Services Bowmanville Canada Jefo Nutrition Inc. Saint-Hyacinthe Canada AlZahal Innovation and Nutrition Kitchener Canada

There are currently no prescribed requirements for B vitamins or choline for dairy cows during the transition period, but many recent studies have shown a variety of benefits from supplying these nutrients to periparturient cows. The purpose of this research effort was to determine the potential benefit of including a rumen protected blend composed of B vitamins (riboflavin, folic acid, vitamin B12) and choline (RPBlend, Jefo Nutrition Inc., St. Hyacinthe, QC, Canada) for dairy cows during the transition period, based on results from 28 on-farm feeding studies (USA-12, Mexico-7, Canada-4, Chile-3, Australia-1, Brazil-1) conducted between 2011 and 2018. All farms participated in monthly herd management record keeping systems and were selected to participate in studies due to their excellent management. Meta-analyses in which risk differences were determined were used to assess the effects of the supplemental RPBlend on health parameters and reproduction. The effect size was used as the determinant of the possible contribution of RPblend on the yields of milk, energy corrected milk (ECM), fat and protein yields during the first four weeks of lactation. Results showed that the inclusion of RPblend reduced (P 0.10) with the inclusion of the BPBlend. The incidence rate of subclinical ketosis, determined as blood beta hydroxy butyric acid greater than 1.2 mM was lower (P (than those not supplemented. The proportion of cows confirmed pregnant by 100 days in milk was greater for cows given the BPBlend (P 0.10) while the yield of milk protein was greater (P

关键词： Lactating Dairy Cows Transition Period Rumen Protection B Vitamins Choline

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杂质含量对7055铝合金微观组织与应力腐蚀性能的影响

杂质含量对7055铝合金微观组织与应力腐蚀性能的影响

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作者： emilie Le Gal 上海交通大学

学位级别：硕士

由于低密度和高强度,7xxx铝合金广泛应用于航空结构材料。然而7xxx铝合金在峰时效(T6)处理状态下的应力腐蚀开裂敏感性高,为了提高合金的抗应力腐蚀开裂性能,人们对热处理工艺进行了大量研究,并采用过时效(T7)和回归再时效(RRA)提高铝... 详细信息

由于低密度和高强度,7xxx铝合金广泛应用于航空结构材料。然而7xxx铝合金在峰时效(T6)处理状态下的应力腐蚀开裂敏感性高,为了提高合金的抗应力腐蚀开裂性能,人们对热处理工艺进行了大量研究,并采用过时效(T7)和回归再时效(RRA)提高铝合金的抗应力腐蚀开裂性能。但是关于杂质元素对铝合金应力腐蚀性能影响的研究极少。近年来发展的7055-T77合金具有高的综合力学性能和较低成本,被用作飞机的主承力构件,如上机翼壁板、桁梁等。本文主要研究杂质(Fe和Si)的含量对7055-RRA铝合金微观组织和应力腐蚀性能的影响。熔炼制备了Fe/Si比恒定、Fe、Si杂质含量不同的7055铝合金,经变形加工与固溶、时效处理后,研究了合金的微观组织与应力腐蚀开裂敏感性。通过扫描电子显微镜结合能谱分析了合金的微观组织,通过慢应变速率拉伸及电化学实验,包括测试开路电压、极化曲线与电化学阻抗谱等,研究了合金的腐蚀性能。主要研究结果如下:1.作为主强化相的Mg(Al,Cu,Zn)2的数量随着杂质元素的增加而减少。随Fe含量增加,富Fe相Al7Cu2Fe与Al3Fe颗粒含量增加,Si含量增加至0.071%,富Si相Mg2Si颗粒含量增加;随着杂质的含量增加,这三种富Fe、富Si相的含量随之增加,并导致主合金相Mg(Al,Cu,Zn)2含量的减少。2.随杂质含量的变化,7055合金微观组织也发生了变化,并影响其力学性能。当Fe/Si=2时,随杂质含量增加,合金的慢应变速率拉伸强度、延伸率及断裂时间减小;合金的抗应力腐蚀开裂性能随杂质含量的增加而有所增加,且对Fe/Si比值不敏感。3.随杂质含量增加,第二相尺寸逐渐粗化且分布更不连续,从而提高了合金的抗应力腐蚀开裂性能。随杂质含量增加,Mg(Al,Cu,Zn)2相的尺寸不变,但面积分数大量减少;而Mg2Si和富Fe相的面积分数增加,但颗粒数量仍然很少,面积分数增加主要是由于颗粒尺寸的粗化,因此颗粒间距无大的变化。4.极化曲线与阻抗谱研究表明,随杂质含量增加,合金腐蚀速率减小,从而限制了开裂速率(阳极溶解机制)和氢气产生。由于晶间相的腐蚀电位不同于基体,所以形成了局部电化学电池。随着总的第二相面积分数的增加,不同种类的第二相所占比例也不同,合金腐蚀速率随之减小,这可能取决于不同颗粒相的特性,如在7055铝合金中,Mg(Al,Cu,Zn)2相的腐蚀速率高于Mg2Si、Al7Cu2Fe和Al3Fe。5.单纯增加Fe含量,使Fe/Si比由0.6(合金1)增加至2(合金2)时,合金延伸率、断裂时间及腐蚀速率的变化趋势,与保持Fe/Si比不变而杂质含量变化带来的力学性能与腐蚀行为的变化趋势完全不同。由此可推想Fe/Si比也是影响7055铝合金性能重要因素。

关键词： 7055铝合金杂质微观组织应力腐蚀开裂腐蚀速率

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Primary CNS Marginal Zone Lymphoma: A Case Report and Review of the Literature

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Open Journal of Pathology 2013年第2期3卷 55-59页

作者： Barina Aqil emilie Rouah Gordana Verstovsek Department of Pathology & Immunology Baylor College of Medicine Houston USA

Primary central nervous system lymphoma (PCNSL) is a rare subtype of extranodal non-Hodgkin lymphoma (NHL) located in the craniospinal axis (affecting CNS, meninges and eye) without evidence of a systemic primary tumor. CNS lymphomas are typically high grade, large cell lymphomas;low grade CNS lymphomas are rare. We encountered a case of 48-year old patient with recurrent episodes of seizures, weakness of bilateral extremities and memory loss. On MRI and CT scan, a 2.0 cm area of ill-defined enhancement with associated edema was present in the left frontal subcortex. The biopsy revealed an infiltrate of small lymphocytes expressing pan B-cell markers and an immunoprofile consistent with an extranodal marginal zone B cell lymphomas of mucosa associated lymphoid tissue (MALT). Clonal rearrangement was demonstrated by PCR analysis. Subsequently, the patient was treated with radiation therapy and responded well, with decrease in the size of the lesion. He is alive and well for 1 year and 3 months up to the date of this report.

关键词： Primary CNS Lymphoma MZL

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