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Journal of Cancer Therapy 2013年第5期4卷 939-943页

作者： Adi Prayitno elyana asnar Suhartono Taat Putra Department of Dental and Mouth Disease Faculty of Medicine University of Sebelas Maret Surakarta Indonesia Department of Pathobiology Faculty of Medicine University of Airlangga Surabaya Indonesia.

Introduction: Now the molecular epidemiology is a new experience. It’s was noted that ninety percent of mouth cancers are squamous cell carcinomas and recorded 0.96% year of all cancers in Indonesia. Human papilloma virus (HPV) was implicated in pathogenesis of cancer. As a remark, that mutations of p53 and c-myc are found 50% in cancer. Objective: Aims of this research were to know the relationship between the mouth squamous cell carcinoma (MSCC) with HPV infection, the presence of p53, and c-myc genes mutation. Methods and Material: Tissue biopsy frozen sections from Benign Mouth Squamous Cell (BMSC) and MSCC patients were collected from Mouth and Dental Department of Muwardi District Hospital in Solo—Indonesia. To amplify L1-HPV gene for fixed the HPV etiology, amplified p53 and c-myc genes continued with SSCP analysis and followed with measurement using densitometer to see mutation existence. The collected data were analyzed with Chi Square Test. Results: None of the sample of patients with BMSC with positive HPV showed p53 gene mutation or c-myc gene. From eleven samples obtained from patients with MSCC who were positive HPV showed 18.2% had mutations in the p53 gene and 9.1% had mutations in c-myc gene. The chisquare test was shown to have significant differences between the MSCC with HPV infection and the presence of p53 and c-myc genes mutation. Conclusion: HPV is a risk ingredient for MSCC.

关键词： HPV p53 and c-myc Mutation MSCC

Heat Shock Protein 40 (Hsp40) and Hsp70 Protein Expression in Oral Squamous Cell Carcinoma (OSCC)

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Journal of Cancer Therapy 2013年第3期4卷 734-741页

作者： Adi Prayitno elyana asnar Okid Parama Astirin Dinar Rosmala Suhartono Taat Putra Departement of Pathobiology Faculty of Medicine University of Airlangga Surabaya Indonesia Department of Biology Faculty of Science and Mathematica University of Sebelas Maret Surakarta Indonesia Department of Dental and Oral Disease Faculty of Medicine University of Sebelas Maret Surakarta Indonesia Department of Ear-Nose-Throat Faculty of Medicine University of Sebelas Maret Surakarta Indonesia

Introduction: As a chaperone, heat shock protein acts as central integrators of protein homeostasis in cell. The form of these functions is to help setting up a complex protein molecular fold (folded protein) in many important settings, such as growth, differentiation, and the ability to live. It has become clear that the control system plays an important role if the folding process fails or an error occurs, causing folding abnormalities and targeted functionality to accumulate. The accumulation of faulty protein folding would harm cells and can result in death. Apparently, there is a correlation between protein folding error with various diseases, such as diabetes mellitus and cancer. Method: We examined protein levels in all samples using Dotblott with monoclonal antibody anti-Hsp40 and anti-Hsp70. Levels of the protein content was read using a densitometer. Modification of Dot Blot was as follows: treatment was conducted with 3 × SSC, added with 20 mL blocking solution, add with total protein samples of 10 mg/ml on nitrocellulose paper, prehybridized, incubated at 70° for 30 seconds, incubated at 70° for 30 seconds with primary antibody anti-Hsp40 or Hsp70 protein and then added with second antibody HRP anti-Hsp40 or Hsp70 protein, treated with 3 × SSC and visualized with TSA HRP, and then administered with streptavidin, biothynil tyramide, and, finally, added with chromogen (DAB) in a confined space. Result: From the analysis of the data using Manova test with Wilk’s Lambda, there were significant differences in the levels of Hsp40 between Benign Oral Lesion (mean 688.31 area) and OSCC (mean 1354.59 area) patients (p 0.070), there was also a highly significant difference in Hsp70 levels between patients who experienced Benign Oral Lesion (mean 529.82 area) and OSCC (mean 1346.32 area) patients (p 0.006). Conclusion: OSCC patients have increased Hsp70 levels, so it is possible that something is going wrong in protein folding. Errors in protein folding result in a

关键词： Chaperone Hsp40 Hsp70 Misfolding OSCC

The Expression of CD8+ and MHC-I in Cervical Cancer with HPV Infection

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Journal of Cancer Therapy 2013年第5期4卷 15-18页

作者： Adi Prayitno elyana asnar Okid Parama Astirin Suhartono Taat Putra Mathematical and Science Faculty of Sebelas Maret University Surakarta Indonesia Medical School of Airlangga University Surabaya Indonesia Medical School of Sebelas Maret University Surakarta Indonesia

Introduction: The immunity against cervical cancers is many unknown. Major histocompatability complex class I (MHC-I) with the alpha domain has binding site for Cytotoxic T cells (CD8+) to target cells. The objective of this experiment is to know the expression of CD8+ and MHC-I in cervical cancer with HPV infection. Method: DNA was isolated from seventeen sample cervical cancer tissues frozen section. Diagnose related with HPV was made by PCR. Paraffin block of the tissues was cut in thoroughly cleaned cryotome and place in glass plate that covered with poly-elysine. The immuno-histochemistry is done with monoclonal antibody again MHC-I and CD8+ with TSA-indirect method. The power of expression was counted the number of positive cells to express the MHC-I and CD8+ every 100 cells per one view look. Result: The Result of this experiment shows that expression of MHC-I and CD8+ in cervical cancer with HPV infection is in mild category (30% - 70%). Conclusion: Immunity against uterine cancer played by MHC-I and CD8+ is in milt category. The experiment that related with uterine cancer immunology is suggested.

关键词： CD8+ MHC-I HPV Cervical Cancer

PKC Is a Target to Modulate the Expression of Receptor Mediated Endocytosis (RME) Mice Macrophages BALB/c for Optimizing the Phagocytosis toward Candida albicans

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Journal of Immune Based Therapies, Vaccines and Antimicrobials 2013年第4期2卷 44-48页

作者： Adi Prayitno elyana asnar Okid Parama Astirin Anief Nur Artanti Meutia Srikandi Fitria Eva Agustina Perwitasari Suhartono Taat Putra Departement of Pathobiology Faculty of Medicine University of Airlangga Surabaya Indonesia Department of Biology Faculty of Mathematics and Natural Science University of Sebelas Maret Surakarta Indonesia Department of Dental and Oral Disease Faculty of Medicine University of Sebelas Maret Surakarta Indonesia Department of Pharmacy Faculty of Mathematics and Natural Science University of Sebelas Maret Surakarta Indonesia

Introduction: The existence of receptor-mediated endocytosis (RME) means that selectivity and selectivity occurs in capturing macromolecules. Protein kinase C (PKC) which can be expressed by almost all cells are proteins important in signal transduction groove that plays a role in a number of cell activity, e.g. phagocytosis. Aims: The purpose of this study is to determine the expression of RME after modulating the PKC which is characterized by the number of Candida albicans cells attached to the surface of macrophages. Methods: Peritoneal macrophages cultured BALB/c mice are treated with PMA and/or bisindolylmaleimides of providing levels of 5 ng/ml to 100 ng/ml for 10 minutes. Then immediately insert Candida albicans and observe every 30 minutes for 120 minutes. The research design used the same subject. Data collected in the form of number of Candida albicans cells attached to the surface of macrophages are analyzed with ANOVA statistical test (one way) to show the differences between treatments. Results: The test shows statistically significant difference in the number of Candida albicans cells attached to the surface of macrophages after administration of various levels of PMA (p 0.001). The higher level of PMA is given, the more active the PKC is, the more RME are formed, the more Candida albicans cells attached to the surface of macrophages. Another result shows statistically significant difference in the number of Candida albicans cells attached to the surface of macrophages after administration of various levels of bisindolylmaleimides (p 0.001). The higher level of bisindolylmaleimide is given, the less active PKC is, and the less RME are formed, the less Candida albicans cells attached to the surface of macrophages. Conclusion: Research shows that activator PKC (PMA) can increase the expression of RME on macrophages. Another research shows that inhibitor PKC (bisindolylmaleimides) can decrease the expression of RME on macrophages.

关键词： PMA Bisindolylmaleimides RME PKC Phagocytosis

Use of siRNA molecular beacons to detect and attenuate mycobacterial infection in macrophages

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World Journal of Experimental Medicine 2015年第3期5卷 164-181页

作者： Remo George Renata Cavalcante Celso Carvalho Jr elyana Marques Jonathan B Waugh M Tino Unlap Center for Teaching and Learning and the Department of Clinical and Diagnostic Sciences University of Alabama at Birmingham

Tuberculosis is one of the leading infectious diseases plaguing mankind and is mediated by the facultative pathogen, Mycobacterium tuberculosis(MTB). Once the pathogen enters the body, it subverts the host immune defenses and thrives for extended periods of time within the host macrophages in the lung granulomas, a condition called latent tuberculosis(LTB). Persons with LTB are prone to reactivation of the disease when the body's immunity is compromised. Currently there are no reliable and effective diagnosis and treatment options for LTB, which necessitates new research in this area. The mycobacterial proteins and genes mediating the adaptive responses inside the macrophage is largely yet to be determined. Recently, it has been shown that the mce operon genes are critical for host cell invasion by the mycobacterium and for establishing a persistent infection in both in vitro and in mouse models of tuberculosis. The Yrb E and Mce proteins which are encoded by the MTB mce operons display high degrees of homology to the permeases and the surface binding protein of the ABC transports, respectively. Similarities in structure and cell surface location impute a role in cell invasion at cholesterol rich regions and immunomodulation. The mce4 operon is also thought to encode a cholesterol transport system that enables the mycobacterium to derive both energy and carbon from the host membrane lipids and possibly generating virulence mediating metabolites, thus enabling the bacteria in its long term survival within the granuloma. Various deletion mutation studies involving individual or whole mce operon genes have shown to be conferring varying degrees of attenuation of infectivity or at times hypervirulence to the host MTB, with the deletion of mce4 A operon gene conferring the greatest degree of attenuation of virulence. Antisense technology using synthetic si RNAs has been used in knocking down genes in bacteria and over the years this has evolvedinto a powerful tool for el

关键词： Mammalian cell entry Molecular beacons siRNA Mycobacterium tuberculosis Macrophages

Biodegradation of carcinogenic textile azo dyes using bacterial isolates of mangrove sediment

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Journal of Coastal Life Medicine 2014年第2期 154-162页

作者： Guru Prasad Srinivasan asnar Sikkanthar Anandajothi Elamaran Caroline R Delma Kumaran Subramaniyan Somasundaram Thirugnanasambandan Somasundaram Faculty of Marine Sciences Center of Advanced Study in Marine BiologyAnnamalai UniversityParangipettaiTNIndia Department of Microbiology Sri Sankara Arts and Science CollegeEnathurKanchipuramIndia

Objective:To evaluate the biodegrading property against carcinogenic azo dyes using bacterial isolates of mangrove ***:The bacterial isolates were subjected to submerged fermentation and their growth kinetics were *** potential strain was characterized using 16S rDNA ***:In the present study,dye degrading bacterial colonies were isolated from the mangrove sediment samples of Parangipettai estuarine area,Tamil *** the 30 morphologically different strains isolated,5 showed antagonistic *** growth kinetics of the two strains,P1 and G1,which showed potent activity were *** particular isolate(P1)showing promising dye degrading potential in the submerged fermentation was further *** strain was identified as Paenibacillus *** 16S rDNA ***:This study reveals the less explored microflora of mangrove *** novel strain may further be analyzed and used in the treatment of effluent from dye industry so as to reduce the impact of carcinogenic contaminants.

关键词： Biodegradation Azo dye Mangrove 16S rDNA Paenibacillus sp